LOCUS       NZ_CP114990             3338 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET19-891 plasmid pCUVET19-891.5,
            complete sequence.
ACCESSION   NZ_CP114990
VERSION     NZ_CP114990.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262092
            Assembly: GCF_030863665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:40:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,014
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 76 of 138
            Pseudo Genes (incomplete)         :: 65 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            Pseudo Genes (short protein)      :: 1 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114990.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET19-891"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET19-891.5"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2019-05-10"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(join(3314..3338,1..170))
                     /locus_tag="O8I28_RS24990"
                     /old_locus_tag="O8I28_24970"
     CDS             complement(join(3314..3338,1..170))
                     /locus_tag="O8I28_RS24990"
                     /old_locus_tag="O8I28_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_021567768.1"
                     /translation="MSEKQRREGFRKAEASLRLEGMDPSGVPRYECLKTRIISGETSYE
                     QGRKEILDYYLRINHKGEE"
                     /name="antitoxin VbhA family protein"
     gene            complement(167..1183)
                     /gene="mobV"
                     /locus_tag="O8I28_RS24995"
                     /old_locus_tag="O8I28_24975"
     CDS             complement(167..1183)
                     /gene="mobV"
                     /locus_tag="O8I28_RS24995"
                     /old_locus_tag="O8I28_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_077820565.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENHCSVSQSADEAMGKLRELLPEKRRKDAVLCVEYVMTASPEWWDKAT
                     PRQQAEFFARSEQWLENKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKR
                     LQSVTEPFKGLSREQMTHILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            1720..2241
                     /locus_tag="O8I28_RS25000"
                     /old_locus_tag="O8I28_24980"
     CDS             1720..2241
                     /locus_tag="O8I28_RS25000"
                     /old_locus_tag="O8I28_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657475.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_099368844.1"
                     /translation="MDNSQIGTPESHEQDNYNFVQVSRAYLKEWRALTRKSPIASEILM
                     YLIENMGRTTNAVVCSYTTLMEITGVSRASVARAVKILKEDRWMQAVKIGNATAYCVNA
                     KAFWQAGRNQKKYAIFQATVIASSSEQESDFLEKSQQDLRYIPFVDLKDRPIVGTDELP
                     PPDQTDLDLD"
                     /name="replication/maintenance protein RepL"
     gene            2587..3297
                     /locus_tag="O8I28_RS25005"
                     /old_locus_tag="O8I28_24985"
     CDS             2587..3297
                     /locus_tag="O8I28_RS25005"
                     /old_locus_tag="O8I28_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021567767.1"
                     /translation="MLYWKKMPSLWIGNRLTEFNSLETSHAIAAIKIYITFCLFGKEED
                     DGVRTVKLTFTEISKIASISRSLVNEGLKLLDNKKLIKYLSPASKRKKIYTVDVDGYIH
                     DGWCKLPMAGIVSEDRTISAFQSMHNRYHFELVALQLYLYLLYARDNFQEHVLARKQTI
                     CEKLACRNVDLNKAITYLIHIGLLKGIKQKAVESYPIELFHDSFYFYVKTGASNALTFK
                     KQPLVNPPEKEIPY"
                     /name="hypothetical protein"
     Typing          complement(273..1183)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.395"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ctaaggtagt aatccaatat ttctttacgc ccttgttcat aacttgtttc gccagagata
       61 atcctcgttt tcaaacattc ataacgaggt actccactag gatccattcc ctcaagtctt
      121 agtgatgctt ccgcttttcg aaacccttca cggcgctgct tttcactcat cggctaatac
      181 ctctgtcagt tttctgacgt tgctggcgtt cctgctcccg ttcaagctgg cgctgttttt
      241 cacgatcgtg attctgttgc tggaacgtct gcgccatcgt cagaatatgg gtcatctgct
      301 cccgggaaag tcctttaaaa ggctctgtaa cgctctgtag gcgtttttgc tgcgaatcca
      361 tagtcttctg tagatcgcgc gctcttttgg cattctgggc gctctgagag gccatagcga
      421 tggttcctgc aaagccatcg ttcactgcct ggcttaaacg tttggctatg gctgcctgat
      481 cttccacgtc tttggtgaaa atacccttcc gcagtacacg cggctccagc gcctccggtg
      541 agatcgatac ctggctacgg gtgccacggt ttatcgattc gtagtagtgc tgaactgtct
      601 gatgcgtggc acggcttccc tcgatgccgc gctcaagacc cagttttttt accgattcag
      661 cgtaggtgct ctgatcgtca cgcatctttg accgcccgcc aataaactct ttcgcggaca
      721 gccgcccatc ctgcgtcaat ggcaccacga acgcagacag gtgcggggtg gcctcgtccc
      781 tgtgaaccac cgccgccaca acgcgatctt tcccgtattt gttctccagc cattgctcag
      841 aacgggcgaa aaactccgcc tgctgccgtg gtgtagcttt gtcccaccac tcagggcttg
      901 ccgtcatgac gtactcgacg cacaacaccg catctttacg gcgtttttca ggcaacagct
      961 cccgcagctt tcccatcgcc tcatctgccg actgagacac agagcagtgg ttttctggtg
     1021 tccgttcagc gtcagcattc ggcgtctcac gctccctgta gcagtgctgc aaagcgctgg
     1081 ccacactgcc cattcccgtc agctttttgc agcgcattat cgcgtaattc gccatgctta
     1141 ccccctcgtt ctggcgtaca tccagccagt cgggggatcg cattgcgatg cctccggcgg
     1201 gccactatca ggatgatagt gtactcacta gactttgctt cgcaaaatcg ttcgggcagc
     1261 aagctgccag aaggtcaaaa tcaacggcgg tctggcaggt ggaaggcgtc gctccgcgcc
     1321 acctcccacc actgccaagc ctgttttatt tgtgcgtcgt caagggtgaa atgcactcgc
     1381 tacgctcgcc cttgacgaaa ttagttccag tgtcagtcct taaaaaaata ccgctcaccg
     1441 caagacatgc tgaggcgcac cgcagcgaag cgaggagtga acagcatgtc gaggaagcgg
     1501 aatgtccact aggcgtacat tgggctacgt atgcacggac ttaatagata tagaaaagga
     1561 gctttcagta aatcgctgat tatattgggt taaaaaaagg gggagtctca ccagccatga
     1621 gacttatgag tctcactagc tatgagactc aagttgacct gttgcatctc taatgataca
     1681 accggtatca aatgggatat tgaaatggtc tatagagcaa tggataattc acagattggc
     1741 actccagagt ctcatgagca ggataactat aatttcgtcc aggttagccg cgcgtatctc
     1801 aaggaatggc gtgcgctgac aaggaaaagc cctattgcct ctgaaatcct tatgtacttg
     1861 attgagaata tgggcaggac aactaatgct gttgtttgca gctatacgac tttgatggag
     1921 ataaccgggg tctctagagc atcagtagca agggcggtta aaatcctgaa ggaagatcga
     1981 tggatgcagg cagtaaaaat aggtaatgcc acagcatatt gtgttaatgc caaagcattt
     2041 tggcaggcag gtagaaatca aaaaaaatac gcaatcttcc aagctaccgt gattgcctcc
     2101 tcctcagagc aagagtctga tttcttagaa aaatcacagc aagatcttcg atatattcct
     2161 tttgtcgatc tcaaagaccg ccctattgtt ggcaccgatg aactcccgcc tccggatcaa
     2221 acagatctag atctcgactg agcctctgaa aaccttcaaa tatggttcac cgtgaaccat
     2281 attgaagttc atttgataac cattttttga tgttaacttt ttgatttaat tataaaaagt
     2341 tatattaatt ttttcccata cctttctaga tagacttttg ctcctgtaga tacagatacg
     2401 cggattactg ggataatcgg tatatcggta aacaggagca gaggtcaaaa tgggtgactt
     2461 agagcgattt gtagagcttg ctgaaaggct gggaaaagtg gcagggtcac ttactagtct
     2521 tgcattggcc atttgtgtgt taattatgat ttatttcact ttcatttccg cataaaggag
     2581 tcactaatgt tatattggaa aaaaatgcca tcactctgga tcggtaatag actgacagag
     2641 tttaatagtt tagagacttc acatgccatt gccgccataa agatatatat aactttttgt
     2701 ctctttggaa aagaagagga tgatggagtg cgaaccgtaa aacttacgtt tacagaaata
     2761 agcaaaatag cttctatcag ccgatctttg gtaaatgaag gtctcaaatt gctcgataat
     2821 aaaaaactaa ttaaatacct atcgcccgcg tcaaaaagaa agaaaatcta tacagtggat
     2881 gttgatgggt acattcatga cggatggtgt aagctgccta tggcaggcat cgttagcgaa
     2941 gacaggacaa tcagcgcctt ccagtcaatg cataatagat accattttga gcttgtagcg
     3001 cttcagctat atttatacct tctatatgcc agagataact tccaggagca tgtgttagct
     3061 agaaaacaaa ctatttgcga aaagcttgct tgccgcaatg tagatttaaa taaagcaata
     3121 acatatttaa tacacatagg tcttttaaaa ggaattaaac aaaaggcagt ggagtcttat
     3181 cccatagaac tttttcatga ttcattttat ttttatgtaa aaacaggagc aagcaatgcg
     3241 cttacattca aaaagcaacc tcttgttaac cctccagaaa aagaaattcc gtactgacat
     3301 acctgagggc gaattactcc tcgcccttat gatttatt
//
