LOCUS       NZ_CP114984             2495 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET18-121 plasmid pCUVET18-121.5,
            complete sequence.
ACCESSION   NZ_CP114984
VERSION     NZ_CP114984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262091
            Assembly: GCF_030863645.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:40:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,973
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,715
            CDSs (with protein)               :: 4,715
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 39 of 139
            Pseudo Genes (incomplete)         :: 101 of 139
            Pseudo Genes (internal stop)      :: 21 of 139
            Pseudo Genes (multiple problems)  :: 19 of 139
            Pseudo Genes (short protein)      :: 1 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114984.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-121"
                     /isolation_source="Kidney stone"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET18-121.5"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-01-25"
                     /collected_by="Pattrarat Chanchaithong"
     gene            783..1010
                     /locus_tag="O8I29_RS24850"
                     /old_locus_tag="O8I29_24825"
     CDS             783..1010
                     /locus_tag="O8I29_RS24850"
                     /old_locus_tag="O8I29_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1376..1561
                     /locus_tag="O8I29_RS24855"
                     /old_locus_tag="O8I29_24830"
     CDS             1376..1561
                     /locus_tag="O8I29_RS24855"
                     /old_locus_tag="O8I29_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1852..2043)
                     /locus_tag="O8I29_RS24860"
                     /old_locus_tag="O8I29_24835"
     CDS             complement(1852..2043)
                     /locus_tag="O8I29_RS24860"
                     /old_locus_tag="O8I29_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1609..1696)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          42..548
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
     Typing          2323..2495
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.857"
                     /coverage="99.0"
                     /evalue="7.14e-47"
ORIGIN
        1 gcccccctga caagcatcac agaatctgac gctcaaatca gtggtggcga aacccgatgc
       61 ggcttaaaga taccaggcgt ttccccctgg tggctccctc gtgctccccc gcattcctgc
      121 ctttcggttt accggtgtca ctccgctgtt atggccgcgg tttctcattc cacgcctgac
      181 actcagttcc gggaaggcag ttcgctccag gatggactgt atgcacgaac cccccgttca
      241 gtccgaccgc tgcgccttat ccggtaacta tcaacttgag tccaacccgg aaagacacga
      301 ctaatcgcca ttggcaacgg ccattggcaa acaggatgtg cgagagatgt ctcaagagtt
      361 cttgaagtgg cggccttagt gaggctacac tgaaaggaca aatttggtga catgtctcgc
      421 acaagactgt taccacggtt aggggatctg ccaggattac ctaaccttcg aaaaaccacc
      481 tgccagggtg gttttttcgt ttacagggca agagattacg cgcggaaaaa aaggatctca
      541 agaagatcct ttataaatta gacatccaac ctcacaaaga ggaatactta aatcatcttg
      601 gtatatgtat cggccggatt acatctcata taagcctcct ccccgcctga caccacactt
      661 tgaccatagc acctcgcgcc taactcgata tacagcccac catcaagcct cttttcacat
      721 aaaaaatgac ctatagttag atggttagtg accaaaactg atatggataa aaggagctgg
      781 ctttgaaaaa gatttttgtt tcagtgttcg cagcggcagt tgcgctttca gccctcaccg
      841 gctgcacgcg cacaagctat gccatacaca ccaatgacgg tcgcactatt gtcagcgacg
      901 gcaaacctaa agaatcagac tcaggcctgc ttggctatac agacgcgaat ggtgtcaaac
      961 agcagatcaa taaaaccgac gttaaagaag tctccgaaat tccgcactga gcatcttctc
     1021 cgaaaaaggc cgctaatgtg gcctttttct ttacctgtgg ttctccgcca aaatcccagc
     1081 aaattgcatc accaaagcta aaagctttca gggttgtcta aaaagcacaa gacataagag
     1141 gaagtgcggt attttataat caagccccca agaatttacc ataacatccg ttgcccgcac
     1201 cgcctgagac gcgttcagcg cgttcctgac gaaaccatga caaaaaccac aacaaaccac
     1261 cccggaaccc gtcagaaacg cgcctgttaa attttaacgg catgcatgac tatgcaccag
     1321 aatgacgcca tgctcttttc acgcaaaaat catcaccaga cggggaaaat caccagtgac
     1381 cagacaggaa tccgccgccc tcaatatggc caaatttatc cgcgcacaga cacttctcct
     1441 ccttgagcgg ctcgagcaga tggatctgga tgaggctgcc ggctgctgtg agcacctgca
     1501 cgatcaggcc gaagcgcttt acgccatgct gaacgcacag ataggcgagg aaaatgcgtg
     1561 aaaatcggtg aacgggtgcg caattcagtg cgcggccgtg aggcgatggc ggggtgtcgg
     1621 ggcgcagccc tgaccagggt atttgtgatg ccggcgcgtg cgcggtatta caaatgcaca
     1681 tcctgtcccg gaacggacac cgggaaacag caaaaaaaac cgggcggcac gcccggaact
     1741 caatcaagtt agattagatt actctcactc gtccataaca gcatcatgga acgacgacca
     1801 ccgtccgtga cggccgcctc gtttaagtat ggacagaaat acagaaaatg ctcaggacga
     1861 aatgtaatga atgcgaacgg attcaagaaa ttcgagcatg acagtcctta cggccggttc
     1921 ggtttcagac aaaatctgcc ggtatgcatc cagcatcatg gctccggcat cccctccggc
     1981 acgccgtagc cagaccgaaa caacggacac aagcaggtgt cgctcatcat cactaagagt
     2041 catcagggct ccggaagaaa aaccaaacat ggcttaccta ctgttaacgg caacgcagaa
     2101 gaaaggttta cagggtagcg ggcagggatg aaaaaaaaaa aattgcagtc aggccggaac
     2161 ggctgctgaa ccggtacagg cgggttccgg gcgcagcgcc ggaccagtca cggagcacta
     2221 gcggagtgta tactggctta agatgtagca tggcagtaac acatagcatt aatgccacat
     2281 aagacaggcg cagaaagtga aagaaaacgc gtcaggagaa aatgtgatac agaatatatt
     2341 ccgcttcctc gctcactgac tcgctccgct cggtcgttcg gctgcggcga gcgggacggg
     2401 cttacggatg gatcagaaat ttcctggggg atgccaggaa gatacttaac gaagaaagca
     2461 taaggccgcg ccaaagccgt ttttccatag gctcc
//
