LOCUS       NZ_CP114983             5411 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET18-121 plasmid pCUVET18-121.4,
            complete sequence.
ACCESSION   NZ_CP114983
VERSION     NZ_CP114983.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262091
            Assembly: GCF_030863645.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:40:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,973
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,715
            CDSs (with protein)               :: 4,715
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 39 of 139
            Pseudo Genes (incomplete)         :: 101 of 139
            Pseudo Genes (internal stop)      :: 21 of 139
            Pseudo Genes (multiple problems)  :: 19 of 139
            Pseudo Genes (short protein)      :: 1 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114983.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-121"
                     /isolation_source="Kidney stone"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET18-121.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-01-25"
                     /collected_by="Pattrarat Chanchaithong"
     gene            436..675
                     /locus_tag="O8I29_RS24825"
                     /old_locus_tag="O8I29_24800"
     CDS             436..675
                     /locus_tag="O8I29_RS24825"
                     /old_locus_tag="O8I29_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_164525734.1"
                     /translation="MEPFEIPAAELADKLEVSQSALSRLLNGKTDLSYEMALRLSKVIG
                     RSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2208..2423)
                     /locus_tag="O8I29_RS24830"
                     /old_locus_tag="O8I29_24805"
     CDS             complement(2208..2423)
                     /locus_tag="O8I29_RS24830"
                     /old_locus_tag="O8I29_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2430..2921)
                     /locus_tag="O8I29_RS24835"
                     /old_locus_tag="O8I29_24810"
     CDS             complement(2430..2921)
                     /locus_tag="O8I29_RS24835"
                     /old_locus_tag="O8I29_24810"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142991550.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLSGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGIQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2956..3660)
                     /locus_tag="O8I29_RS24840"
                     /pseudo=""
     CDS             complement(<2956..3660)
                     /locus_tag="O8I29_RS24840"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3599..3922)
                     /locus_tag="O8I29_RS24845"
                     /old_locus_tag="O8I29_24820"
     CDS             complement(3599..3922)
                     /locus_tag="O8I29_RS24845"
                     /old_locus_tag="O8I29_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4036..4209)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          4133..4720
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.54"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(2117..3609)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagcaaaac tccgaagccc tttatgcttt atgtttttta tcatcaatca tctccataaa
       61 attttcgtcg atatctatct ctataaacag aaaacaattt gacagcgtcc atttgaaccc
      121 cttgcattat taaaataatt gacgtaactt tgtctctgag gtcgattacc tcgtttgact
      181 tccaagagcg ctaaccgtac aatatggcgc cttgaaaaag tcggtaaaga caccatatca
      241 gcggtagctt tctgctccac cgagcatcct aaagcttact gctttttttg tcaataacag
      301 acacttgcgt attgtgcaat gcgcaacgcg aaagaaacaa aatgttttgt gtaatcaaat
      361 acaaaaccaa tagaggaagg catgataaat ctttacgatc ctgtacaccc cggtgaattc
      421 atcagggaaa tgttcatgga accgttcgaa atacctgccg cagagttagc tgataagctt
      481 gaagtatcac aatctgcatt aagtagattg ctcaatggaa aaactgatct tagttatgag
      541 atggctcttc gactatctaa agttataggc cgctctgcag agtcgtgggt taatatgcag
      601 gctcagtaca gcctctcgaa aagtcgtaag actttatccg agacgctaga acaccttcat
      661 ccgatgtttc cataagtaaa agcccgctcg tgcgggcttt ttgccgtaaa accccccgca
      721 tacctacctt tagtttgatt tttagttcag ttcttagatc ttgaaatgat tgttgtttga
      781 acaccaataa gatgcccata caaggctcta aaaaggagaa caaaatggca atttatgtag
      841 gaccacatgg caaaataggt cgtgatggtg gctcattcca agaatgtgac atcaacacaa
      901 acactcatac tgttacccgc aaatatggac gatactgcac cattcgcgat aatgagttcg
      961 caccaccaac gagcgcacct ggcaggtact ggctacagat ctaccgaacc ccagacaaca
     1021 ctgagtaaca gaagcgccag taacgacaaa gcccgcataa gcgggctttg tctcttacaa
     1081 aattcctcgt cgtcgcaatt cagcttctat cgcctcacat gcggcatcca caatctccgt
     1141 gttgtagtac ctggacatgc cactgctaat gatatcgtcc attagggcac ggttatgagc
     1201 taaggtacgt cgtaagtatt caggggtatg ttgctccatc ggattctcct tttcatgact
     1261 gagcattatt gcttacctgt atttatcccc gtcagtgctg gtcacgttga aaagccctaa
     1321 aaaaccactt ttttagctgc cttaatcatt gaaaaatggc acttaaatcc acatctatcg
     1381 cgctggggaa atcgcccgca cacagccact gataaaataa aagtgagata aaatggatcc
     1441 aatggataag atttgcgatg tttgtgccct ccctgccagc gcctgtacct gtggcttctg
     1501 cgatgactgc aacgagaagc tggcagactg cgtctgtgag cgctgcagcg attgtggaaa
     1561 tttagatgag cactgtagct gctcacgttg cgacgagtgc ggtgaaaaac tggatgagtg
     1621 catatgtgag gagtgcgaag aatgtctccg cagtccacat gattgtatct gcgaaaactg
     1681 acctaaggcc gaattctctt tcaagagcga ccgggacggt cgctcttatt taccataaca
     1741 tccgttgggc gcaccgctga aacggggttg gctgcgtttc cggtgccaga cgcgagaaag
     1801 tggcgacaga cataacgaaa aaacaccaga aacgcagccg ctgaatatta cactggtgca
     1861 tagtcatgca gccagatgca tggatgttaa agtcacacta aaatccgcgc caatgcctta
     1921 aaaaccgaca aatgcttctc cagccagtcc cacagctccg ccgccagata caggcagtta
     1981 agcaatgatt caataagatt taacagcttc atttcccgct cctgaataag tcgtgacctt
     2041 tttgatcacg aacctattta gcgggagaaa taagagcccc tcttcgtgcg ggttgtcagc
     2101 tgcctggatg gtttaaagtg tcggcccgcg gtaggttttc tgcggctggc gctccagctc
     2161 cagtgttttc tgatactcat tctccctctg ccggagctcg atggcattca gttcccggac
     2221 aagcggcgca gctgctcgct taaactcagc acctgcgtgc tcaagtccga cacgcgctcg
     2281 ttcagcaccg cgttctcccg ttgcataagc ccacacatgc tccgccattc cgcgaaggcg
     2341 ctctcccatt cgtccagcct ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc
     2401 aactgctttt ccagctctgt cattttcagc tactcctgtt tcaggcggat ccatgtcgtc
     2461 ccgtcgtaag ggatggcttc ggtttgtatc cccggcggca ggataagaaa cctccggccg
     2521 tcgctgctct cctgatagcg cactccccac gtcctggcgg tcattttcgc cagggtatct
     2581 tcctgctgac gaagatgcgt gtaattgtcg gttatctgct gcccctgcca ccacagaatg
     2641 ctcccgctcg tgccgctcag cagcgctgac accatcagga tggtgagcca tgtccgcccg
     2701 gcagtccgca gtacgctccg ccggtttttc tccatcgcct cgctcatact ctgctcgtgg
     2761 gcgaggatgg cgtcgctgat tctcctcgcg cttgcgctca gttccgctct gacagactgc
     2821 tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac tctgctgctg cgctttcgat
     2881 tgctgctcta agtccttcgc cagcgttaaa agactgctca tagatggctc ccttcagtcg
     2941 gatgtttcgc cccccgtccg gatcggcaat gctgatactg cttttcgtgg tgcgcaccac
     3001 ctcaaaacct gcaccttcaa gagtgtcagt gacatcctga cggcttttca gctccccgga
     3061 cgaagcaagg gtcagcagtc cccgggtaat ggcttctgcg gcctcctgct tcgttttcgg
     3121 aagcccggac ggcgtgacca gcgcccggcg gttttccggc gcgttcgggt cgtgcagccc
     3181 cagtctgccg ttcacgatag tctgccaggc atcgatgcgc ggccggtcgg cgcggtcgta
     3241 gtaaggctgg agccgcctgc cggtcagcag ctcggtattg gggatcagaa agttcagctc
     3301 cagacgcccc ttgtccgcgt gctccaccca cagaatgctg tactgatctt tatcgagtcc
     3361 gggcatcaga acccgctcga agctggccat cagtttttcc cgctggccgg gaggcagatc
     3421 ggcctccgca aacgacagaa cgccggaggt gtatttctta acgtaaggcg acgcgtcaat
     3481 cagctcccgg acttcttccg gcttcccctg caggacggtg gcgccttcac gctgccggtc
     3541 tttacccagc agatagtcca ccggcccggc gccaccgccg cggccgcgcg gatgaaattt
     3601 aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg cagccgctca aggccggcat
     3661 cgatggccat cagcgccgcc acaatctgta ccctgtcatg gccggtgccg ccgccggcgt
     3721 taacacggcg ggctatctgg ttcaggttat tgcccatgcc ggcaagctgg cgcagcagcg
     3781 ccggcgagag tgaggggagc tttcctgaac gtgccggctt ttcgtccagg cagatctgcc
     3841 tcatccacgc cgccagctgc ctgccgtcac agcgctcgag cagccgccgg tgctcgtcct
     3901 ccgtcaccca catggtgagc atcttgctcc gtttctctga caaaccggcc tcctgataac
     3961 gccgggacgg gcgggaaagc ctttcccggt cggcgcgggg ttggacaaac cgcaggcgcg
     4021 tcaggctttt ggcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt
     4081 gtatactggc ttactatgtt ggcacggatg agggtgtcag tgaagtgctt catgtggcag
     4141 gagaaaaaag gcggcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     4201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggaaa tggcttacga
     4261 acggggcgga aatttcctgg aggatgctag gaagaagctt aacagggaag cgagaaggcc
     4321 acggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     4381 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggt
     4441 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcac tccgctgtta
     4501 tggccgcgtt tatctcattc cacgcctgac actcagttcc gggaaggcag ttcgctccaa
     4561 gctggactgt atgcacgaac cccccgttca gtccgaccgc agcgtcttat ccggtaacta
     4621 tcgacttgag tccaacccgg aaagacacaa caaaacgcca ctggctgcgg ccattggtaa
     4681 gagggtgtac aagagattac tgatgcagag ttcttgaagt cagagccata ctgaggctaa
     4741 actggaagga cagaatttgg tttcagcggt cttgtacacc ctgttaccac ggttcagaag
     4801 ttccccaact tactgaaccc tcgaaaaaac cgcctcccca ggcggttttt tcgtttcgaa
     4861 cataagatac gctttgtcga tttaatcagt caaggaagaa ccctgaaagc ctcaatcgct
     4921 tacagaatta ttcttgttag agacatgttt ctgcaccgta tcaacaccag tacacaaaac
     4981 agctgcaaac aggaaccatc cccacccatc taggccacga acagcaagaa ccccagcaat
     5041 gactacacaa agcaaaggga acaacaaaat cataaaggct ctcataaatc ctccagaggt
     5101 acttattttt gaataaagtc cctaatccat ccatattgcc actaggatgg attagtaact
     5161 tacttattga ttactaatgg taatcttcaa gatcaacgtc taagacgttt ggttcttcca
     5221 ccttgaaaac caccctccag tttccgttaa cctctacgga aaactcacct ttacgttcac
     5281 cttttagggg gtgacacccc agcgccctta catccacaat atcttgaaga catgttgcgg
     5341 tgcttagcgt agtcaatatc atgcgaagtt ttttcgctaa gtgaggctga attccggcgg
     5401 tgttaccgtt t
//
