LOCUS       NZ_CP114800             2558 bp    DNA     circular CON 28-APR-2024
DEFINITION  Providencia sp. 21OH12SH02B-Prov plasmid p21OH12SH02B-4, complete
            sequence.
ACCESSION   NZ_CP114800
VERSION     NZ_CP114800.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32252641
            Assembly: GCF_027362595.1
KEYWORDS    RefSeq.
SOURCE      Providencia sp. 21OH12SH02B-Prov
  ORGANISM  Providencia sp. 21OH12SH02B-Prov
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Parker,E.M., Mollenkopf,D.F., Ballash,G.A., Li,C. and Wittum,T.E.
  TITLE     Transcontinental Dissemination of Enterobacterales Harboring
            blaNDM-1 in Retail Frozen Shrimp
  JOURNAL   Foodborne Pathog Dis (2024) In press
   PUBMED   38563789
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2022) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2022
            Assembly Method                   :: HGAP v. 11
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 380x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027362595.1-RS_2024_04_28
            Annotation Date                   :: 04/28/2024 10:31:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,261
            CDSs (total)                      :: 4,154
            Genes (coding)                    :: 4,058
            CDSs (with protein)               :: 4,058
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 35 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 31 of 96
            Pseudo Genes (multiple problems)  :: 28 of 96
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114800.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Providencia sp. 21OH12SH02B-Prov"
                     /mol_type="genomic DNA"
                     /strain="21OH12SH02B-Prov"
                     /isolation_source="shrimp"
                     /db_xref="taxon:3015951"
                     /plasmid="p21OH12SH02B-4"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-12-03"
                     /collected_by="The Ohio State University"
     gene            complement(join(2395..2558,1..127))
                     /locus_tag="O7C57_RS21295"
                     /old_locus_tag="O7C57_21295"
     CDS             complement(join(2395..2558,1..127))
                     /locus_tag="O7C57_RS21295"
                     /old_locus_tag="O7C57_21295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_269724039.1"
                     /translation="MTFRQETTDEIIKRLGLKTLKESLNKTIFLLSFGGFKSMQKVFDE
                     TFDEITEARKYRSWQRITDCLNEHTTHNLSVLTVKTMYKRAKKKRENNKTE"
                     /name="hypothetical protein"
     gene            complement(124..360)
                     /locus_tag="O7C57_RS21830"
                     /old_locus_tag="O7C57_21300"
     CDS             complement(124..360)
                     /locus_tag="O7C57_RS21830"
                     /old_locus_tag="O7C57_21300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152716331.1"
                     /translation="MNFDELDGFCDALTKKYKSRQVCIRMYHNDLTFCINMYGWKNIIK
                     KIKEKTGLEIKQINLQNQYYREIKKLALKADKK"
                     /name="hypothetical protein"
     gene            1132..1776
                     /locus_tag="O7C57_RS21305"
                     /old_locus_tag="O7C57_21305"
     CDS             1132..1776
                     /locus_tag="O7C57_RS21305"
                     /old_locus_tag="O7C57_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     ARG             1133..1773
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          884..1038
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.172"
                     /coverage="99.0"
                     /evalue="1.84e-47"
ORIGIN
        1 aaaccttttg catacttttg aacccaccaa atgacagcag gaaaatggtt ttattgagcg
       61 attctttcag tgtttttaag cctaatcgtt taataatctc atctgttgtt tcttgcctaa
      121 atgtcatttt ttatctgcct tcaaagctag ctttttaatc tctctataat attgattttg
      181 aagatttatt tgttttatct caagaccggt tttttctttg atttttttta ttatgttttt
      241 ccatccgtac atgtttatgc aaaatgttaa gtcgttatgg tacatgcgga tacatacctg
      301 ccttgattta tatttctttg tcagtgcatc gcaaaaacca tctaattcat cgaaattcaa
      361 cttcattata tcctcgctga tctcattaca tgcttcaggc tattttacgt aatgtagtat
      421 tatttttgta ttatatccaa ctatcactgt ttcttttttg agctcatcta gcttcagtat
      481 tatttttgat gttatttagt attatttttg atggctttca gtatggctaa tgattgtttt
      541 cagtattaat cgattgaatt agtattattt ttgatggctt tcagtatgag gaatgatgac
      601 gttcagtatt atttttgatg ttgcgtcaaa aaataaaggc acgacatcga ccgcagacaa
      661 acgggtacat aatggggaca ttattgccgt ttgtctacag tcgcgtactc agggggatcc
      721 ctgatacccc gaagcctcta cgagtctttt ttctgacgcg ctgcgcttgt ccaacgattc
      781 acagacagat aaaaatttta tctgcccgtt caccgtttat gagtcaaacg ggaagataat
      841 tattttgaag gttaaggggt aatttttgtg gctaataaaa taatgttaaa aacgaagcca
      901 aacggcacag aaaccgttct aaactttcaa cgtggttttg ggtgtttgat agggtgatcg
      961 tgtcttaaaa tcgttttgag gggctttaat gcggatgaac gggcggtttt agtaaactaa
     1021 ctcgcccgtt taacataaaa gggattgtat gaatcaattt tagctagagt ttaaggttgt
     1081 tcaaattaat gtacaatgat tacactgtat aaataaccag gtatagcatg tatggaaaag
     1141 cactttatca atgaaaagtt ttcacgagat caatttacgg ggaatagagt taaaaatatt
     1201 gccttttcaa attgtgattt ttcaggggtt gatttaactg atactgaatt tgttgattgt
     1261 agtttttacg acaggaatag cttggaaggg tgtgatttta atagagccaa actaaaaaac
     1321 gctagcttta aaagctgcga tttatcaatg agtaatttta aaaacattag cgccttaggt
     1381 cttgaaatta gtgagtgttt agctcaagga gctgattttc gaggggctaa ttttatgaat
     1441 atgataacta caaggtcatg gttttgtagt gcttatataa ccaagacaaa tcttagttac
     1501 gctaattttt ctagagtcat attagaaaag tgcgaactgt gggaaaatcg ctggaatggc
     1561 actgtgataa ctggcgccgt gtttcgtggc tccgatcttt cttgtgggga gttttcatcg
     1621 tttgattggt ctttggctga ttttactggt tgtgatttaa cgggtggggc gcttggcgag
     1681 cttgatgcaa ggcgaattaa tttagatgga gtgaagttgg atggagagca ggcgcttcag
     1741 cttgttgaga gtttaggtgt tattgttcac cgataaaatc taggtaaaaa acgcctaatg
     1801 ccccaatgtg gtactaatca aaacggagcg aagcgacagc atctttctgc ctttgctttt
     1861 ctccatgatc agtgattggg tcatggcttc ttagcttatt ccattagcta agtctggtac
     1921 ttctggttag gttgggcttc tttcgacggt gcaatccaag aaatccctag tccgttgatt
     1981 ggtgctctta tcaggtacgc actgtaatcg ttcgataccg cagcgtgatt cggaggcaga
     2041 gccggaattt cgttttccgg tacgttatta ccacaatttg cctgtttatc ctttcggaga
     2101 ccgggctaag tccgagtcaa tcaagtggca catagagagt tattgtcttt gaatggtttt
     2161 aaggaagtcg tgacaagcga gcttcccgtt gctaagattg gattgtctag atcgaagtct
     2221 tagcaacgta tgtgcaagat ttagagtaac agagccccat gaccctgtca cggggctttg
     2281 ttatatctgt agcctgataa attccatgaa ttcaagctac aacgcttgtc gcaagatgaa
     2341 aatttatttt tcatcgagga agcggaagtg catttgatgc tgattggtac tcggtcactc
     2401 cgttttatta ttttcccgtt ttttcttggc gcgtttgtac atggttttta cggtaagcac
     2461 gcttaaatta tgggttgtat gctcatttaa acaatcagtt atgcgttgcc atgaccggta
     2521 ttttctggct tcagtgattt catcaaaggt ttcatcaa
//
