LOCUS       NZ_CP114799             4136 bp    DNA     circular CON 28-APR-2024
DEFINITION  Providencia sp. 21OH12SH02B-Prov plasmid p21OH12SH02B-3, complete
            sequence.
ACCESSION   NZ_CP114799
VERSION     NZ_CP114799.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32252641
            Assembly: GCF_027362595.1
KEYWORDS    RefSeq.
SOURCE      Providencia sp. 21OH12SH02B-Prov
  ORGANISM  Providencia sp. 21OH12SH02B-Prov
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Parker,E.M., Mollenkopf,D.F., Ballash,G.A., Li,C. and Wittum,T.E.
  TITLE     Transcontinental Dissemination of Enterobacterales Harboring
            blaNDM-1 in Retail Frozen Shrimp
  JOURNAL   Foodborne Pathog Dis (2024) In press
   PUBMED   38563789
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2022) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2022
            Assembly Method                   :: HGAP v. 11
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 380x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027362595.1-RS_2024_04_28
            Annotation Date                   :: 04/28/2024 10:31:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,261
            CDSs (total)                      :: 4,154
            Genes (coding)                    :: 4,058
            CDSs (with protein)               :: 4,058
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 35 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 31 of 96
            Pseudo Genes (multiple problems)  :: 28 of 96
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114799.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Providencia sp. 21OH12SH02B-Prov"
                     /mol_type="genomic DNA"
                     /strain="21OH12SH02B-Prov"
                     /isolation_source="shrimp"
                     /db_xref="taxon:3015951"
                     /plasmid="p21OH12SH02B-3"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-12-03"
                     /collected_by="The Ohio State University"
     gene            complement(join(3910..4136,1..184))
                     /locus_tag="O7C57_RS21275"
                     /old_locus_tag="O7C57_21275"
     CDS             complement(join(3910..4136,1..184))
                     /locus_tag="O7C57_RS21275"
                     /old_locus_tag="O7C57_21275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_269724036.1"
                     /translation="MSFFNLFKQNGKSWIQHKAVNGIAPEVWEYKHGFSALWFLGDSFT
                     LEDGRLNNLIKHWADIVTVSLYVSIGNESITSNANIDITPLQGAKARVRIPNELTEKII
                     SAFSLNKTVVFMVNGKHCGEYSGKNFPYHFKK"
                     /name="hypothetical protein"
     gene            749..1894
                     /locus_tag="O7C57_RS21280"
                     /old_locus_tag="O7C57_21280"
     CDS             749..1894
                     /locus_tag="O7C57_RS21280"
                     /old_locus_tag="O7C57_21280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_269724037.1"
                     /translation="MMNRIALLVSLFFIVSCDDRRDAIELVKSIVTPIDNSITLGKAYD
                     NRRVCLSTSWNSYTDSRDRLIVDYTCSLDVKKINDVVFSYLNNQMNKSISFYDKELNKL
                     NDINVSNVEKINDLTHLMSVLYKINDSGLLDDLYKNISILNSHGDGDKYFDVNSNYDLR
                     VSSIVSSELLTNEIQRTFSVYRDEFNELGRIDMATLLNNVEHSLLNLQAFLVKENVFNN
                     DYRFQNIYNHSYRAYIDEYLNSNSYDLIDTELKTRVSNLESEIKKTDNNKINIEKQKDK
                     IIILSSEIQREFDIVSVNQNIYFSIVNDEPKLFGCEFKVKTNYKEYILKPKYCMDMSYS
                     TDVDKYFYALSDLIYTNDIKNNFLEYVNELQVEYEKDIKNR"
                     /name="hypothetical protein"
     gene            complement(1905..2486)
                     /locus_tag="O7C57_RS21285"
                     /old_locus_tag="O7C57_21285"
     CDS             complement(1905..2486)
                     /locus_tag="O7C57_RS21285"
                     /old_locus_tag="O7C57_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217637.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_269724038.1"
                     /translation="MSRIFIYCRCSKLEQDTANQVIAIRKYRPEFRNIPDNRVIEEIVS
                     GGTPAMKRPRFASLVNEKLEQGDTLVVLKLDRLGRDNIDVQQTILLLHEKRINVVCLDL
                     PVADLMSAEGRLMLQLFAAFAEFEKSRIIERTQEGLEKAKANGVRLGRPKGSSVHSDAI
                     QAAKEAGLSQSKVAKRLGIGIATVKRHWRR"
                     /name="recombinase family protein"
     gene            complement(2620..3429)
                     /locus_tag="O7C57_RS21290"
                     /old_locus_tag="O7C57_21290"
     CDS             complement(2620..3429)
                     /locus_tag="O7C57_RS21290"
                     /old_locus_tag="O7C57_21290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_269724035.1"
                     /translation="MTKRITFNIKNTKTIDTIDRYCLTKNNISRSEVIASLLNSVAPIL
                     DEISDKYQAAQAIDNKLLSLFKTEVTSQIQKQHGEVPAKLHCLEIWNNHIRCGFTPLDD
                     FVSQTPKEHTRKDNNIGIHEKEDIENTIRQVMKVVTGAKLALFIYTKRKVERQNHLALA
                     GGISQTLFLKNNIYHDYLFDIQNAKVLNADDLIFFGINDSLKKNKIKHNPKYLCWIPVF
                     YTGLNMGYATVAHKAKVIIVPVLHREDVPESVFTERPKDVVIVNPFE"
                     /name="hypothetical protein"
ORIGIN
        1 ctatatctgc ccaatgtttt attaaattat ttaagcgccc gtcctcaagg gtaaagctat
       61 cacccagaaa ccataatgca ctaaaaccgt gtttatactc ccatacttcg ggtgctattc
      121 cattgacagc tttgtgctga atccagcttt ttccattttg tttaaataga ttgaaaaatg
      181 acatattgcc tctttaaagc cataaataac ctaatgcccc aatgtggtac caattataac
      241 ggagcgaagc gacagcgtct tcctgccttt gcttttctcc atgatcggtt attgggtcat
      301 ggcttcttag ctgcttaatc tagctaagac tggttaggtt gggcttcttt cgacggtgca
      361 atccaagaaa tccctagtcc gttgattggt gctcttatca ggtacgcact gtaatctttc
      421 gataccgcag cgtgattcgg aggcagagcc ggaatttcgt tttccggtac gttttactac
      481 cacaatttgc ctgtttatcc tttcggagac cgggctaagt ccgagtcaat caagtggtgc
      541 atttgagtta atgtcgttga atggttttaa ggaagtagtg acaagctagc ttcccattgc
      601 taagattaga ttgctgaagt cgaatcgtta gcaatgaatg caccgattta gattagcaga
      661 gccggaaaga tgtcacaatc ttttcggctc tgtgccattc aggatttatt tttattgttt
      721 ttttacttaa tgtgttttaa atggatttat gatgaatcgt attgctttac ttgtttcact
      781 attttttatt gtgtcatgtg atgatcgtcg tgatgcgatt gaattggtta agtcaatagt
      841 tacccctatt gataatagca ttacgttagg taaagcttat gataatagac gtgtctgtct
      901 ttctacatcg tggaatagct atactgactc tagggataga ttaattgttg attatacgtg
      961 tagtttggat gttaaaaaaa taaatgatgt tgttttttct tatttaaata atcaaatgaa
     1021 caaatcgata tctttttatg ataaagaatt aaataaatta aatgacatca atgtatctaa
     1081 tgtagaaaaa attaacgatt taacacattt aatgtcggtt ttatataaaa taaatgatag
     1141 tgggttactg gacgatttat ataaaaatat ttctatatta aatagtcatg gggacggtga
     1201 taaatatttt gatgttaact caaattatga tttaagagtt tcatctatag tcagtagtga
     1261 attgcttacg aatgagattc aaagaacctt ttcagtttat agagatgaat ttaatgaatt
     1321 aggtcgtatt gatatggcaa cattgttaaa taatgttgaa catagtcttt tgaatttgca
     1381 agcttttctt gtcaaagaga atgtttttaa caatgattac agatttcaaa atatttataa
     1441 tcattcctat cgtgcatata ttgatgaata tttaaatagt aacagttacg atttgataga
     1501 tactgagttg aaaacaagag tttcaaattt agaaagtgaa ataaaaaaaa cagataataa
     1561 taaaataaac attgaaaaac aaaaggataa aataataatt ttatcctcag aaattcaaag
     1621 ggaattcgat atagttagcg ttaatcagaa tatttatttt tcaattgtaa atgatgagcc
     1681 taaattattt ggttgtgaat ttaaagttaa aactaattat aaggagtata tacttaaacc
     1741 aaaatactgt atggatatgt catactctac agatgtagat aaatactttt atgcattatc
     1801 cgatttgata tatacaaatg atataaaaaa taatttctta gagtatgtga atgaattaca
     1861 agttgaatat gaaaaagaca tcaaaaatag ataattgtaa tgtgtcatct tctccaatgt
     1921 ctcttaacgg tagctattcc aatacctaat ctcttcgcga ctttactctg gctcaagcct
     1981 gcttcttttg ctgcctgtat cgcatcagaa tgaacgctag accccttagg cctacctaac
     2041 cttaccccat tggctttagc cttctctaag ccttcttgag tgcgttctat gattcttgat
     2101 ttttcaaact ctgcaaacgc tgcaaataat tgcagcatca atctaccttc agcactcatt
     2161 aagtcagcta caggaagatc taaacaaaca acattgattc ttttttcgtg taacaacagt
     2221 attgtttgct gaacatcaat attatccctg cctaacctat ctagctttaa cactaccaat
     2281 gtatcccctt gctcaagctt ttcattcaca agactagcaa aacgaggtct cttcattgcg
     2341 ggggtaccac cactgacaat ctcttcaatt actcgattat caggaatatt cctaaattcg
     2401 ggtcgatatt ttcgaatagc tatcacttga ttagctgtgt cctgctctaa tttagaacac
     2461 ctgcaataaa taaaaattct actcacaaca cccccttaaa cgggtatcaa aatacggtat
     2521 cgttaaatgt agtggtatcg atattgttaa tcaatagttt tttgatacca ataatcaaac
     2581 ccgatcaccg tatcagtaat tgttcgttta gtgataccac tattcaaatg gatttacgat
     2641 caccacatct ttaggtcttt cagtaaaaac gctttctgga acatcttctc gatgtaagac
     2701 aggtacaata atcactttgg ctttatgagc tactgttgca tatcccatgt ttaaaccggt
     2761 gtaaaaaaca ggtatccagc aaaggtattt tggattgtgc tttatcttgt tttttttcaa
     2821 cgagtcgttt atgccaaaaa aaataaggtc atctgcattc agaacttttg cgttctgaat
     2881 atcaaagaga taatcatggt aaatgttatt ttttaggaat agcgtttggc taattcctcc
     2941 agctaaggct aaatggtttt ggcgttcaac ttttcttttg gtgtaaatga aaagagctaa
     3001 tttagcccct gtaactactt tcattacttg tctaatagta ttctctatat cttctttttc
     3061 atgaatacct atgttgttgt ctttgcgtgt gtgctccttt ggggtttggg atacaaaatc
     3121 atctaatggt gtaaatccac atcgaatatg attattccat atttcaaggc agtgtagctt
     3181 agctggaacc tctccatgtt gtttttgaat ttgacttgtt acctctgttt taaatagtga
     3241 tagaagcttg ttatcgatag cttgagctgc ttgatactta tctgagattt catcgaggat
     3301 gggagctaca gagtttagta gtgatgctat aacttcggat ctggatatat tgtttttggt
     3361 tagacagtat cgatcaatag tgtctatcgt ttttgtattt tttatattga atgtgattct
     3421 ttttgtcata tcattttcag ttattcatat tcctagtata tgcataactt atgctattta
     3481 tcttattata atcaataagt atttttttgt tgattaaaaa atgtttagat atatgttatc
     3541 tataaaaaaa ctaagtttag gctatggact gagagctagc ttagtactca gtattataat
     3601 gccttacccc ctttccccca tacagacaac agaataagga tgtcgtactg tccagtctat
     3661 caagcttact tacactacac ttatctacac ttcgttccgc taagtttcgt tccatccagc
     3721 tttcagggct gtccagtact cctcttaaca ccaactactt ttttctttca taagatatag
     3781 agtaaccttg ttttaaatta aaaaatgaga gtgggttgga cttgtgtttt tatagaatag
     3841 agttgcttta acgggcggtt tttagttact agctcgcccg ttaaacaaaa agggattaca
     3901 gaacctattt tattttttaa aatgatatgg aaaattcttt ccagagtact ctccacagtg
     3961 ttttccattt accataaaaa caactgtttt atttagtgaa aatgcgctta tgattttttc
     4021 tgtcaattca ttcggtatac gtactcgggc ttttgcacct tgaagtgggg ttatatctat
     4081 attggcatta cttgttatcg attcattacc tatagataca tatagagata cggtaa
//
