LOCUS       NZ_CP114622             6188 bp    DNA     circular CON 15-FEB-2024
DEFINITION  Enterococcus faecium strain EF0656 plasmid pEF0656-5, complete
            sequence.
ACCESSION   NZ_CP114622
VERSION     NZ_CP114622.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32302292
            Assembly: GCF_027286245.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Luo,Q., Zhang,G. and Tian,W.
  TITLE     Complete genome sequence of Enterococcus faecium strain EF0656
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Luo,Q., Zhang,G. and Tian,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2022) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP v. 4; FALCON v. 0.3; Canu v. 0.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.82x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/15/2024 07:03:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,955
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 46 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 32 of 142
            Pseudo Genes (multiple problems)  :: 24 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114622.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="EF0656"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEF0656-5"
                     /geo_loc_name="China: Zhuhai"
                     /lat_lon="22.22 N 113.55 E"
                     /collection_date="2022"
                     /collected_by="Wenfang Tian"
     gene            complement(join(6096..6188,1..195))
                     /locus_tag="O4N81_RS15120"
                     /old_locus_tag="O4N81_15120"
     CDS             complement(join(6096..6188,1..195))
                     /locus_tag="O4N81_RS15120"
                     /old_locus_tag="O4N81_15120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            complement(215..439)
                     /locus_tag="O4N81_RS15125"
                     /old_locus_tag="O4N81_15125"
     CDS             complement(215..439)
                     /locus_tag="O4N81_RS15125"
                     /old_locus_tag="O4N81_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            complement(909..1079)
                     /locus_tag="O4N81_RS15130"
                     /old_locus_tag="O4N81_15130"
     CDS             complement(909..1079)
                     /locus_tag="O4N81_RS15130"
                     /old_locus_tag="O4N81_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            complement(1094..1627)
                     /locus_tag="O4N81_RS15135"
                     /old_locus_tag="O4N81_15135"
     CDS             complement(1094..1627)
                     /locus_tag="O4N81_RS15135"
                     /old_locus_tag="O4N81_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1648..2592)
                     /locus_tag="O4N81_RS15140"
                     /old_locus_tag="O4N81_15140"
     CDS             complement(1648..2592)
                     /locus_tag="O4N81_RS15140"
                     /old_locus_tag="O4N81_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            3229..3315
                     /locus_tag="O4N81_RS15145"
                     /old_locus_tag="O4N81_15145"
     CDS             3229..3315
                     /locus_tag="O4N81_RS15145"
                     /old_locus_tag="O4N81_15145"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3436..4125)
                     /locus_tag="O4N81_RS15150"
                     /old_locus_tag="O4N81_15150"
     CDS             complement(3436..4125)
                     /locus_tag="O4N81_RS15150"
                     /old_locus_tag="O4N81_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4370..5284)
                     /locus_tag="O4N81_RS15155"
                     /old_locus_tag="O4N81_15155"
     CDS             complement(4370..5284)
                     /locus_tag="O4N81_RS15155"
                     /old_locus_tag="O4N81_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5266..5646)
                     /locus_tag="O4N81_RS15160"
                     /old_locus_tag="O4N81_15160"
     CDS             complement(5266..5646)
                     /locus_tag="O4N81_RS15160"
                     /old_locus_tag="O4N81_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     BGC             1..5439
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1649..2592)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4374..5284)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatttatgt tttaaaatat atcttccaag aaaactactt aatttcatac taacttcacc
       61 aacacttttt ccctcactga tttctttaga ataaagaaag agcttttctt ttaagtcagg
      121 atagtcatct atagttgctt cattgtatac tttactaatt gcatttaaaa gtttttcttc
      181 tttagtaaaa tccattataa taacctccct accactacct tctaggtgcc catggaccat
      241 gattaaccca accattaaca ataatttttc caatttctcc tttagcttga ttccaatcta
      301 cccaacattt ttctttgtta caataaagac catttccata gtaagtagct gcatcaactt
      361 ttattcctgt accgatgcca gctagacaag ttcctaatat tcctagaata acacaatgtt
      421 ttaatacttt ctttttcatg attgtttttc cttttcgttt ttttaaaatt gcttgttata
      481 ttaaataatc ttagtaattt tattatataa cattttatta tataacaatt tattatataa
      541 caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata aaattataag
      601 atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac gcctgtacca
      661 agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc tctttttaag
      721 gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg gtcatttttt
      781 aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat attcatttat
      841 tattcttttc taaggttaat aataggataa tctctttcag aaagactgct cgtacgagca
      901 gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact gttgtagtaa
      961 ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga taataacatt
     1021 gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt ttttgtcata
     1081 agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt gtttgttcgt
     1141 ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac tcttccgtat
     1201 aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc agggtgagtt
     1261 gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg agggcaagtt
     1321 gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt gcaaccacgt
     1381 ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc aacttatcta
     1441 gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc aatacaacct
     1501 tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata tgtttcgata
     1561 ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag gtttcttgtg
     1621 gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat cagataataa
     1681 actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag cttttttctc
     1741 tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct gtttgatttt
     1801 ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta aagccaatgc
     1861 tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt ttttgatctc
     1921 aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc cgtatttctt
     1981 tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt ttataactcg
     2041 tttatctata tcaggttgct tataactttt aggaatatct aataattcaa gaaagtcttc
     2101 tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa acatagtctt
     2161 tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc actcgtctaa
     2221 attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag gattttctac
     2281 gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa gcccagactc
     2341 acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta aatcttctac
     2401 aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt caaacgtaaa
     2461 acgaactgtc ttatcccctt tttcacgcat tctagacacg atggaaaaaa acaaattcat
     2521 ttcaaccggt gtaaattttc ttaaaggaat tgtatttaat tcatggtggt attttacaat
     2581 ctcattagcc atataatcac ctcaatccaa ttatgaatga cattttttaa atgtcaagac
     2641 attcatattt gacgttcaaa cagacaaaat gtccttcata aacagacaaa atgtccttca
     2701 taaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataacat tctgtatcca
     2761 ttggtagagt aaggacacag cttacggtaa agaggtttaa aaagaggttt aaaaagagat
     2821 ataaaaagag gggggctaat tttcccgcct aaaaaccact tcttttttct ttaaacaggt
     2881 tcgcgcattt ttcaaaactg ccaatccaaa accttgcttc ctacggtcgc caaaaaggtg
     2941 tgatcgctac gctctcacat tgtactgcgc agtcgcctaa aggctccttt gctaaaaaca
     3001 tgttggtaaa gaggcctttg agcggttctg aatgcttatc taagccgttt taagacagtc
     3061 ttggataaaa atccttatct agctttaaag cgccttacag gcggtcaggg caagccctaa
     3121 caaccctaaa ataagcgata cttaaaaaca ccacacaagt ttaatttttt aagatataat
     3181 aacttgtcgc ctatccgaca gtcttgcgac tggaaggagg tgtatctaat gtttgaattg
     3241 ttcatttgcc cccttcttat agaagtttgt gtctctttat tcagttattg gttgaataac
     3301 cgaaacaaga agtaaaatca ataaaggcga catcaacctg aaaaaaagcc ccagagaatc
     3361 cgacctctct ggggctttcg tgtatctaat gtttgatcat ttgcatgttt agtttaacat
     3421 gattgccgat caaattcaat tttgctgttg ctgatctaac cattccttga cactttttag
     3481 ataatttaat gccgtttctt ttttcgttgg attttgtatg ttcgttgctt gctgaagcag
     3541 tgctttataa cgttcgtagg taactgattg ttgccgttct tttcgttcct gttcactagt
     3601 tgaaaaaggg attgtatcac tgaataaatt ataacaagct tcttctgtct gcgtatcttt
     3661 aaaatattgc acgtcttttt tggttcgctc atagttgtct tgaacctctt tttgctgttt
     3721 ttctggcaag ttttgataaa gacgagtgag tttttccatg tttttatccg tcaaacgttt
     3781 ttcttgattc acaaaaatat cgttcatttc ctcataacaa gtggtaaact cttccatttt
     3841 ttgtgttaac acttgctcct ttttcttttt atcacatgct gcttcatact tttgagtgat
     3901 atcccctaat aaaatacttc ttgaaatacg tttatttccc tctaaaacgc cgaataaacc
     3961 acttaaaacg attgataatg taaatataac ccctggcaat aaaaacctct taaatcgaat
     4021 tttagagcct tttaatgaat atcttgtttg ataagcctta tagctaagcc aaataattcc
     4081 taaaatactg gcaaaaatga gtagataggg taaatagaat gccatttcta tttattctcc
     4141 ccataccagc caaaaggcta acagtgtcat gattaagcaa cataaggggg caagaatatc
     4201 cattgatcgc tgcataatta cttttttctg ttctttattt cgctggttta atagcgcatg
     4261 gtggaatttt ttgacttgtg aatcaattgt agtttgtatt ctttctttgg tataaggtgc
     4321 aagaaaagca cgttgtttct ttatttcatt ttctatgtct tgtaacgttt tagaacgata
     4381 attcttgttg gtagtctgct cctctttcaa cgtgttggtc atggtttctt cgagtcggtc
     4441 taatttccgg ttgttcagtc gtaagagttc gttgttctct tcgtacttct tttgcagttt
     4501 cgcgaatgct agctcgagct cttcgtagct cgttggttcg ttgttgattg taggctcgtt
     4561 ctcgtctgtc cagctcatgt aaaatcgtct ccttttcgta atcctttcct agtttgttgg
     4621 atcgtacttt tttctttttg tggatcaatt cgtaagtaaa atttttccca cgattcaagc
     4681 aatgaatgcc gaatgtttta agagaatcac aaaattggtt atacgttcga gtttttttgt
     4741 ttttcattgt ttgatcaatc gcaaaccgaa tttcgtcttt ccaaacaggt tcattttttt
     4801 cttttaattt gatttcagac atggttcgtt tttcgggttt cttccctcgt tcagtgattg
     4861 ttaagtcatg ttcaagacaa atctcgtcat ttaggtcata acatttgttt aaaaatgctt
     4921 gaccatgggc ttgaaatttg ttcccagttt ccagattgac ggagttaatg acaatgtggt
     4981 tatgaatatg gtctttgtct gtatgtgtgt aaatcgcgat ttggtggtca ggggctattt
     5041 ttttcgcata ctctgcccct agttgattgg ctttttcagc ggtaatttct cctggtcgaa
     5101 aagactggat caataagtgt gcttgtacac gatcgtcttt tccatacagc atgcgaattt
     5161 gtttcatttc gcttttggca taatcaatgt cacagtttat ccctgttttg acggttgctc
     5221 gtggttcggc gtaattgatc gctcggctac aagaagtggt tcggcttact ttaactgttg
     5281 ccatatcttt tgtagctcct tttgcatgat ttttaggttt tcggataagc gttgggcgtc
     5341 gccaggatca acgtcgtgtt ggtgtgtgtt gcaccacttg gcaatttgat tgatattccc
     5401 gccagctttg gctaaatcgc gcgcgattgc ctgagcaatg tctggcgcca ctttcggtgt
     5461 caccagacgc gcgttttgtg ccttggcttt cacgaaagcc ggcacactca tttgcaaagc
     5521 ttcagctgac tgcttcagct ttgcaaattc ggattcgctc actcgaaaac tgatttgttt
     5581 tggctcggat cgataggtat attttttaga atgattgcta gccaaatatt gattttgttc
     5641 gctcataagt cccacccttt cagtacgtcc tacggacgta tcgttcgcaa taccattgta
     5701 cctaaaaagg tacaataagt aaagctcact aaagctcact aaagggcttg gcaagctcta
     5761 tatttgctag ccaaatacct acgtatttgg tgcaaataac cgcttgtcaa aggggcgcaa
     5821 gctccccctt gaatccccct cccccccact ctccacaata caaaaaagac tagcaaatgc
     5881 tagccacttt ttgtaaagtt tcgcttgttt caccttcggt gaaattgttg caaatcaata
     5941 attaggtaga gctacatttt tttcgttgtt caaagattct tatgccattt actattaata
     6001 aatctataaa tatgataatt tttcggctct ataatttatg ggactgatga atcagaattg
     6061 attcttcagt cccattttca ttttattcca aagaattaac tccaaatacc aatagaagcc
     6121 catcctctat atacttgaga ttccttacta acaatttctt gtaattctat taaagattta
     6181 ggtaatcc
//
