LOCUS       NZ_CP114621             9372 bp    DNA     circular CON 15-FEB-2024
DEFINITION  Enterococcus faecium strain EF0656 plasmid pEF0656-4, complete
            sequence.
ACCESSION   NZ_CP114621
VERSION     NZ_CP114621.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32302292
            Assembly: GCF_027286245.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Luo,Q., Zhang,G. and Tian,W.
  TITLE     Complete genome sequence of Enterococcus faecium strain EF0656
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Luo,Q., Zhang,G. and Tian,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2022) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP v. 4; FALCON v. 0.3; Canu v. 0.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.82x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/15/2024 07:03:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,955
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 46 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 32 of 142
            Pseudo Genes (multiple problems)  :: 24 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114621.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="EF0656"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEF0656-4"
                     /geo_loc_name="China: Zhuhai"
                     /lat_lon="22.22 N 113.55 E"
                     /collection_date="2022"
                     /collected_by="Wenfang Tian"
     gene            complement(394..597)
                     /locus_tag="O4N81_RS15070"
                     /old_locus_tag="O4N81_15070"
     CDS             complement(394..597)
                     /locus_tag="O4N81_RS15070"
                     /old_locus_tag="O4N81_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(609..1142)
                     /locus_tag="O4N81_RS15075"
                     /old_locus_tag="O4N81_15075"
     CDS             complement(609..1142)
                     /locus_tag="O4N81_RS15075"
                     /old_locus_tag="O4N81_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(1593..1766)
                     /locus_tag="O4N81_RS15080"
                     /old_locus_tag="O4N81_15080"
     CDS             complement(1593..1766)
                     /locus_tag="O4N81_RS15080"
                     /old_locus_tag="O4N81_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2451..2990)
                     /locus_tag="O4N81_RS15085"
                     /old_locus_tag="O4N81_15085"
     CDS             complement(2451..2990)
                     /locus_tag="O4N81_RS15085"
                     /old_locus_tag="O4N81_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(3006..3929)
                     /locus_tag="O4N81_RS15090"
                     /old_locus_tag="O4N81_15090"
     CDS             complement(3006..3929)
                     /locus_tag="O4N81_RS15090"
                     /old_locus_tag="O4N81_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            complement(5080..5283)
                     /locus_tag="O4N81_RS15095"
                     /old_locus_tag="O4N81_15095"
     CDS             complement(5080..5283)
                     /locus_tag="O4N81_RS15095"
                     /old_locus_tag="O4N81_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(5295..5828)
                     /locus_tag="O4N81_RS15100"
                     /old_locus_tag="O4N81_15100"
     CDS             complement(5295..5828)
                     /locus_tag="O4N81_RS15100"
                     /old_locus_tag="O4N81_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(6279..6452)
                     /locus_tag="O4N81_RS15105"
                     /old_locus_tag="O4N81_15105"
     CDS             complement(6279..6452)
                     /locus_tag="O4N81_RS15105"
                     /old_locus_tag="O4N81_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(7137..7676)
                     /locus_tag="O4N81_RS15110"
                     /old_locus_tag="O4N81_15110"
     CDS             complement(7137..7676)
                     /locus_tag="O4N81_RS15110"
                     /old_locus_tag="O4N81_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(7692..8615)
                     /locus_tag="O4N81_RS15115"
                     /old_locus_tag="O4N81_15115"
     CDS             complement(7692..8615)
                     /locus_tag="O4N81_RS15115"
                     /old_locus_tag="O4N81_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     Typing          complement(3007..3929)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7693..8615)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatttcgc tcgattatta gcatatattt tttagctttt ttgctagcca aattttgcat
       61 taatttgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg
      121 aaagtacctt taaaggtact ttctacaaaa ctcactaaaa gggtttggca agctctatat
      181 ttgctagcca aatacctccg tatttggtgc aaataaccgc ttgtcaaagg agcgcaagct
      241 cccccttgaa tccccctcaa aatccacgct ccacaataca aaaaaaggct agcatttgct
      301 agccactttt tgtaaagttt cgcttatttc accgaaggtg aaattgttgc tttgtaatat
      361 tcaaacaacc ctattcttct aaaatgtatc gatttatttt tttttagact ttattatgtc
      421 taagacaact aagattaaca ctaaaaataa accagatttg tataattgcc ctccgcttag
      481 catattaaca attatagcaa cagctatagc aaatataaaa atacgaatag tagctatttg
      541 cggattcttt ttagtaaatg tttttaatga ttttaatgat ttagatatca aaaacatcac
      601 tccttaactt ataacgttga atatctagta gctacaactc ttccagctgc atcatatccc
      661 ctttggtaat aaaatccaat ccagccatta ccatttttgt cccattcttt tgcaactgca
      721 ttccctggag aagcaccttt tttagtccaa cttgcacctg ttaacaaagc accaattgcc
      781 caacctggga taccaaacat agaagtgcta gctttccctc ctgcccaaaa ggcagcatta
      841 atagcattag cagctactcc agtggttaca gcaatatttg tatattcctt tttgccccat
      901 cttgcaactg cagctcttgt catgatatct gaagaatttg caaaatcttc cgcactataa
      961 ccatcctcat atttagttac gaccatacca tcttcagtgt actcaataac tacattttca
     1021 gtagcattgt tttcgtttaa tgataccact tgggaatttt ctgaagcata aactatagta
     1081 gttgacggta aaacaactgc cccaatagtt aacaaagcta cccctaaagt aaattttttc
     1141 atagaatttc ctcctgatac agaaaaatat atagaaaacg ttttctttgt gaaaatcata
     1201 aaacaaaagg tataggcttg tcaatatttt atttagtaaa gataatttat atttttgata
     1261 atgtggtata gttagaatgc attaggaaaa tatgttatat taaattcttg ttgacataat
     1321 gtgtactatg ccaagtagcc tctctaccaa gggatacagg ggctcacttt gtgattttcg
     1381 attggtctaa gtcaaaagct ctttttaagg cttgattttc tgcagttgct tctttccgtt
     1441 tctttgtgaa tttttaccag tcatttttta ctggggtgtt atttttcata aaatatgata
     1501 aatttttggt agcgcttcat aagcgcgttg ctttttcaag aataaacact cattattttt
     1561 taactggctg ctcgaaagag cagtcttttt tattatttat ccgtctcttt tacaaacaat
     1621 tcttcgttag gactgttgta ataatttgaa tttaatttaa gttctccgtt ggaattgttt
     1681 tttccaatta cggataatag catggggcta aaacctaata ttcctagaga aatagaaccc
     1741 agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa cgctaccact
     1801 tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg aacggatata
     1861 aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg aagttactct
     1921 aacttttttt gcttgtggtg cttggtatgt tttcttcgcc gaccgtttag gttcttgacc
     1981 attttctctt ataagtggga tttttaccac gttgagagaa aatggtcaag aacctgggag
     2041 gcggagaatg cccttaatac caagcaccgc aagcctttcc catactttat tcgatgatcc
     2101 cacgatgatc tcataatgat tctacaatga ccctacgatg atcaaaattc tcaaaaaaaa
     2161 taaacgaaat ttcaaacgat ttttttgaaa ttttgttaaa atcacatttg tttttttatg
     2221 actttttcaa caaaatcgat tagaatttat tttgtttttc caccaaaaat tgagtgagat
     2281 ttcaacctat tttaagcaat aagtcaccat atagtttgat agtttacaac tataaacatt
     2341 gattgtttac agttgtaaac tattttttct ttctcataaa aatttgagtg atctccccat
     2401 caggtagtac aactttttcc tctactatcc gaactaactt ctagaaaatt ttagagattg
     2461 gttctattag atttccaaat ttgccaccac tttttttcaa taagtggttg tgtattgtct
     2521 gctttttttt cagaaaaggc agttgattcg gttgttggag tttcttctgt ataagttaaa
     2581 tggagctgtt cttgtaggtt ctcaatttgt tggttcgctt gtagagtgag ttgttgttgc
     2641 tggtcgagta atttttgcat ttgttttatt tgattatctt tttcttgtag ctgtatatct
     2701 tttcgattta actgtttttc taggaaagaa aggagtttta attcggaaac ttggttggtg
     2761 tttttatggt ttttggtttt tgatttttcg ccaactagtt ttcgatttaa gtttgcgtta
     2821 tcgactaaaa acattgattt tatagctttt tgaccggttt cgttaatcac aaacttgttt
     2881 ccgttttttg caaaccaaag ttttcgattt ttgtcagaaa cttttttgtt aattgctgtt
     2941 ttactaactc ctaattcttc cgcaagttct ttgattgttt tatctgccat aaatactcct
     3001 taagcttaat cgaataagtt ttgtaaattt cctagtaaat cttgtttgag tttgtttgaa
     3061 agttctcgtt tttctgcctg tttttcttca gtttgttgtt gttgtttaaa atcagcttca
     3121 tgagtgccta agggaagatt tagtattcga tctttagcgc gccatttttc ttctcgtgtt
     3181 aattctccat tgtgatcaat attaaataat tttatacgta aatcttcttg tttccctttg
     3241 gaaaaatcgt cagcatggtt aatttcagct ttccaagtaa aacgataacc gataacaggt
     3301 ttacctcgac ctttaccata ttttttacga atagttaatc ctttaaataa gggagtgagt
     3361 tcttctttaa ttggccttaa gataaatttg tcaatatttg acggactatt ccaataactt
     3421 ttaggaatat ctagtaaatc aaaaaatttt tctttggaaa attcagtcca gccttgagtt
     3481 cgatattgtt ttaataatcg aaacatagtt tttgaatatg tgctatccaa attagtaaat
     3541 tgctctagat taaatcttgt ccagttcttt aattgattaa aaagtccttt aaattctgga
     3601 ttaactgaaa ttataacaag ttgtttatct cgtataatgc tatattttgt gaaagcaaca
     3661 aatttagtta aagtatttcc atcatcattc caagcattaa tacttaataa tttgtcatac
     3721 gtgctactaa gatcttttat aaattgttcc ccacgttctg tataaccgct tagttttttc
     3781 aaatcgtgaa atgaaaaaat aatctcatct acaccttttt cacgtacacg tgaagcaatt
     3841 gaaaaaaata agttcatttc acgactatta aattttctca gaggaatatg atttaatcta
     3901 ttttcatatt ttacaatttc atttgccata agcttctcct acttttataa agtatgaaat
     3961 aaaagtaaca tactttataa aaatactcaa taatgtcgga aaacctactt gaaaagcgta
     4021 gaaaacctac ttgaaaagcg tagaaaacct acttgaaaag cgtagaaaac ctacttgaaa
     4081 aaactctgaa ttgcttggga gagtaaggac acagccgaca taaaagagat ataaaaagag
     4141 atataaaaag agatataaaa agaggggagg ctaattttcc cgcctaaaaa atcactcctt
     4201 ttttctctaa acaggttcgc gaatttatca aaactgccaa ttccaaacct tgctccctac
     4261 ggttcgctaa aaaggtgtga tcgctctgct ctcacattat actgcgcagt cgcctccggc
     4321 tcctttgcta aaagccgttc ataaggaacc ctttgagaag ctctgagcgt tcgtttaaga
     4381 cattttaaga cagtcctagt ataaatccct tatcttgttc ttaaaacgcc ttacaggcgg
     4441 tcagggcaag ccctaacaac cctaaaattg tctaaatgcc ctctaaaatt cgatttaagc
     4501 actcttaaac ctattcttat atatttatat acttaggcgt ttcaaacgtc ttaaaacgca
     4561 aattagagct tgataaagat atttgctcgt gtgcctagac ttttaaaaaa gtcggcacac
     4621 gccatgacaa agctgaaata aactgcttca gctttgtcat ggcggattcg ctcacttgaa
     4681 aaagtgttta tttcgctcga ttattagcat atatttttta gcttttttgc tagccaaatt
     4741 ttgcattaat ttgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgt
     4801 aatgtgaaag tacctttaaa ggtactttct acaaaactca ctaaaagggt ttggcaagct
     4861 ctatatttgc tagccaaata cctccgtatt tggtgcaaat aaccgcttgt caaaggagcg
     4921 caagctcccc cttgaatccc cctcaaaatc cacgctccac aatacaaaaa aaggctagca
     4981 tttgctagcc actttttgta aagtttcgct tatttcaccg aaggtgaaat tgttgctttg
     5041 taatattcaa acaaccctat tcttctaaaa tgtatcgatt tatttttttt tagactttat
     5101 tatgtctaag acaactaaga ttaacactaa aaataaacca gatttgtata attgccctcc
     5161 gcttagcata ttaacaatta tagcaacagc tatagcaaat ataaaaatac gaatagtagc
     5221 tatttgcgga ttctttttag taaatgtttt taatgatttt aatgatttag atatcaaaaa
     5281 catcactcct taacttataa cgttgaatat ctagtagcta caactcttcc agctgcatca
     5341 tatccccttt ggtaataaaa tccaatccag ccattaccat ttttgtccca ttcttttgca
     5401 actgcattcc ctggagaagc acctttttta gtccaacttg cacctgttaa caaagcacca
     5461 attgcccaac ctgggatacc aaacatagaa gtgctagctt tccctcctgc ccaaaaggca
     5521 gcattaatag cattagcagc tactccagtg gttacagcaa tatttgtata ttcctttttg
     5581 ccccatcttg caactgcagc tcttgtcatg atatctgaag aatttgcaaa atcttccgca
     5641 ctataaccat cctcatattt agttacgacc ataccatctt cagtgtactc aataactaca
     5701 ttttcagtag cattgttttc gtttaatgat accacttggg aattttctga agcataaact
     5761 atagtagttg acggtaaaac aactgcccca atagttaaca aagctacccc taaagtaaat
     5821 tttttcatag aatttcctcc tgatacagaa aaatatatag aaaacgtttt ctttgtgaaa
     5881 atcataaaac aaaaggtata ggcttgtcaa tattttattt agtaaagata atttatattt
     5941 ttgataatgt ggtatagtta gaatgcatta ggaaaatatg ttatattaaa ttcttgttga
     6001 cataatgtgt actatgccaa gtagcctctc taccaaggga tacaggggct cactttgtga
     6061 ttttcgattg gtctaagtca aaagctcttt ttaaggcttg attttctgca gttgcttctt
     6121 tccgtttctt tgtgaatttt taccagtcat tttttactgg ggtgttattt ttcataaaat
     6181 atgataaatt tttggtagcg cttcataagc gcgttgcttt ttcaagaata aacactcatt
     6241 attttttaac tggctgctcg aaagagcagt cttttttatt atttatccgt ctcttttaca
     6301 aacaattctt cgttaggact gttgtaataa tttgaattta atttaagttc tccgttggaa
     6361 ttgttttttc caattacgga taatagcatg gggctaaaac ctaatattcc tagagaaata
     6421 gaacccagta ccaacacagt tttttttaac ataggttata cctccacttt ttttaacgct
     6481 accacttgta tacacatttt aaatactagc cttcagttta tcaaatgttt aaattgaacg
     6541 gatataaatc aaaattaaat tttcaaaagt tgttctaaaa aatttttctt ttttggaagt
     6601 tactctaact ttttttgctt gtggtgcttg gtatgttttc ttcgccgacc gtttaggttc
     6661 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     6721 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     6781 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     6841 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     6901 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     6961 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     7021 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     7081 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     7141 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     7201 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     7261 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     7321 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     7381 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     7441 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     7501 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     7561 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     7621 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     7681 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     7741 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     7801 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     7861 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     7921 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     7981 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     8041 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     8101 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     8161 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     8221 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     8281 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     8341 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     8401 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     8461 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     8521 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     8581 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     8641 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     8701 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     8761 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     8821 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     8881 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     8941 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     9001 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     9061 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     9121 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     9181 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     9241 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     9301 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     9361 cttgaaaaag tg
//
