LOCUS       NZ_CP114360             3223 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Klebsiella variicola strain 2022CK-00567 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP114360
VERSION     NZ_CP114360.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31592762
            Assembly: GCF_026069065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00567
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 3223)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 61.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 04:43:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,793
            Genes (coding)                    :: 5,628
            CDSs (with protein)               :: 5,628
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 61 of 165
            Pseudo Genes (incomplete)         :: 103 of 165
            Pseudo Genes (internal stop)      :: 38 of 165
            Pseudo Genes (multiple problems)  :: 31 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114360.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00567"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:244366"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2022-08-15"
     gene            426..749
                     /locus_tag="OPU51_RS29495"
                     /old_locus_tag="OPU51_29495"
     CDS             426..749
                     /locus_tag="OPU51_RS29495"
                     /old_locus_tag="OPU51_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            958..>1392
                     /locus_tag="OPU51_RS29500"
                     /old_locus_tag="OPU51_29500"
                     /pseudo=""
     CDS             958..>1392
                     /locus_tag="OPU51_RS29500"
                     /old_locus_tag="OPU51_29500"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1427..1912
                     /locus_tag="OPU51_RS29505"
                     /old_locus_tag="OPU51_29505"
     CDS             1427..1912
                     /locus_tag="OPU51_RS29505"
                     /old_locus_tag="OPU51_29505"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1916..2131
                     /locus_tag="OPU51_RS29510"
                     /old_locus_tag="OPU51_29510"
     CDS             1916..2131
                     /locus_tag="OPU51_RS29510"
                     /old_locus_tag="OPU51_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2299..2631
                     /locus_tag="OPU51_RS29515"
                     /old_locus_tag="OPU51_29515"
     CDS             2299..2631
                     /locus_tag="OPU51_RS29515"
                     /old_locus_tag="OPU51_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             2300..2631
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          139..330
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(2666..3188)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.143"
                     /coverage="99.0"
                     /evalue="8.31e-121"
     Typing          740..2229
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggggcggag cctatggaaa aacggcaact ccgcatcctt ttcttggcct ttcctgcttc
       61 gagcggcatt tgcttatccc gtaatctgtg agctggtaac gcccgccgca gtctcacggc
      121 aggagcgtag cgaccgagtg agcgaggagg cgtcatttca tcaccacctg cattttgcac
      181 ttacgcccgg cagccgcatt tttcccctgt cacatgaagc actttcataa attgccactc
      241 cgtcataaca tgtttagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
      301 cgccccgaaa cccgctaaaa ccactgccgc gcctgcggct tgtccaacac cgtgccgacc
      361 gggaaaagat ttcccgtccg tcccggcgtt atcaggaggc cggattggca gacaaacgca
      421 gcaaaatgct caccatgtgg gtgactgagg acgagcaccg gcgtctgctg gagcgctgcg
      481 agggtaagca actcgcggcc tggatgcggc agacgtgcct ggacgagaag cccgcacgcg
      541 ccggcaaact tccgtcgatc tcgccggcgc tgcttcgtca gcttgccggc atggggaaca
      601 accttaacca gattgcccgc caggttaacg ccggtggtgg aagcggacac gaccgcgtgc
      661 agattgtcgc cgcgctgatg gccatcgatg ccggactcga gcggttgcgg catgccgtac
      721 tggaaaaggg tgctgatgat gatcgttaag tttcatcctc gggggcgcgg cggcggcggc
      781 gggccggtcg attatctgct gggtaaagat cgccagcgcg acggcgccag cgttctgcag
      841 gggaagccgg atgaagtccg ggagcttatc gacgcctcgc cctacgccaa aaagtacact
      901 tccggcgttc tgtcttttgc cgaacaggat ttaccgcccg gccagcgcga aaagctgatg
      961 gcgagtttcg agcgggttct gatgcccgga ctcgataaag accagtacag cgtgctgtgg
     1021 gttgagcacc gggacaaggg gcggctggag ctgaacttcc tgatcccgaa cacggagctt
     1081 ctgaccggca agcggctaca gccgtattac gaccgcgccg accgtccgcg catcgatgcc
     1141 tggcagacca tagtgaacgg caggctgggg ctgcatgacc cgaacgcgcc ggagaaccgg
     1201 cgggtgctgg tctcgccttc cgcgctgccg gaagcgaagc aggaagccgc ccaggcgatt
     1261 acgagcggcc tgcttgccct tgcctcatcc ggggagctga aaacgcgtca ggacgtcact
     1321 gaggcgctgg aaagcgcagg ttttgaggtg gtgcgcacca caaaaagcag catcagcatt
     1381 gccgacccgg acggggggcg aaacatccga ctgaagggag ccatctatga acagtctttt
     1441 aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagacta ccggcgagat
     1501 gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcga
     1561 gaagagaatc agcgccgcca tcctcgacca cgaccggaag ctgtcctcag ccatgagcca
     1621 gcgcacgaaa gggatgctgc gcatggtcag ccagacgtgg ctgaccatcg ttctggtctc
     1681 cgcgctgctg atagcgtcga gcgcgggcat tctgtggtgg caggggcagc agatactcga
     1741 gaactatacg accatccggg agcagaagag cacgcaggcc atgctgtcag agaggaacag
     1801 cggcgtacag ctctcgacct gcggcgagca gagacgccgc tgcgtgaggg tgaacccgga
     1861 agcgggacag ttcggagagg actcgagctg gatgatactg gcggggaaat agcacatgac
     1921 ggagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
     1981 gctggacgag tgggagaacg ccttcgcgga atggcggacg atgtctggtc ttattcaacg
     2041 ggagaacgcg gcgctgaacg agcgcgtcac ggtcttgagc aggcaggtgc agagtttgag
     2101 cgagcagctg cgccggttgt cgaaaggctg aacgcgatag aaactcaccg gcagcaggag
     2161 cgggcggtgc agcaccagaa agcgctggag ctggaacgat cgcagtggca gcatcatggt
     2221 ccttcgctat gataagccgg gttctattaa ttagttgtta tgtaaggata tttaaatttt
     2281 tcaacatgag gtatttttat gaatacatat attttcctgg gaggggctat tgtggctgaa
     2341 gtcataggca ccacctttat gaaatattca gaaggattta cacgcctgtg gccgtctgta
     2401 ggaactgcaa taagttattg cgctgccttt tatctgcttt cccaaacatt ggaaaatata
     2461 cctaccggta ttgcttacgc aatttggtct ggtgcgggaa ttgtactaat aagcctagct
     2521 ggttggttgg ttagcggtca aaaacttgat tggcccgcaa ttgcaggaat gttccttatt
     2581 tgcgttggag ttttggttat taatattttt tcaaaatctt ctgcgcatta actttttacc
     2641 gaagcatatt ggccaaagca aaaggatttc cttgagatcc tttctttctg cgcgtaatct
     2701 ttttccctgt aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaattcaga
     2761 gctaccaact ctttgttccg atggtaactg gctttcacgg agcgcagata cctaaactgt
     2821 cctttcagtg tagccgcact caggctttca cttcaagaac tctgtaggca ttctctacaa
     2881 tctctgctcc gtgaacccag ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     2941 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3001 gttcgtgcat acagtccatc ctggagcgaa ctgcctaccc ggaaccgagt ttcaggcgtg
     3061 gaatgagaaa ccgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaatgc
     3121 gggggagcac gagggagcca ccagggggaa acgcctggta tctttaagcc gcatcgggtt
     3181 tcgccaccgc tgatttgagc gtcattttcg tgatactcgt cag
//
