LOCUS       NZ_CP114359             3672 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Klebsiella variicola strain 2022CK-00567 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP114359
VERSION     NZ_CP114359.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31592762
            Assembly: GCF_026069065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00567
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 3672)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 61.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 04:43:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,793
            Genes (coding)                    :: 5,628
            CDSs (with protein)               :: 5,628
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 61 of 165
            Pseudo Genes (incomplete)         :: 103 of 165
            Pseudo Genes (internal stop)      :: 38 of 165
            Pseudo Genes (multiple problems)  :: 31 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114359.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00567"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:244366"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2022-08-15"
     gene            complement(295..576)
                     /locus_tag="OPU51_RS29465"
                     /old_locus_tag="OPU51_29465"
     CDS             complement(295..576)
                     /locus_tag="OPU51_RS29465"
                     /old_locus_tag="OPU51_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            913..1206
                     /locus_tag="OPU51_RS29470"
                     /old_locus_tag="OPU51_29470"
     CDS             913..1206
                     /locus_tag="OPU51_RS29470"
                     /old_locus_tag="OPU51_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            1270..1572
                     /locus_tag="OPU51_RS29475"
                     /old_locus_tag="OPU51_29475"
     CDS             1270..1572
                     /locus_tag="OPU51_RS29475"
                     /old_locus_tag="OPU51_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_023317863.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMNDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     gene            complement(2356..2757)
                     /locus_tag="OPU51_RS29480"
                     /old_locus_tag="OPU51_29480"
     CDS             complement(2356..2757)
                     /locus_tag="OPU51_RS29480"
                     /old_locus_tag="OPU51_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040242827.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGARNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2757..2948)
                     /locus_tag="OPU51_RS29485"
                     /old_locus_tag="OPU51_29485"
     CDS             complement(2757..2948)
                     /locus_tag="OPU51_RS29485"
                     /old_locus_tag="OPU51_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040242829.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3363..3509
                     /locus_tag="OPU51_RS29490"
                     /old_locus_tag="OPU51_29490"
     CDS             3363..3509
                     /locus_tag="OPU51_RS29490"
                     /old_locus_tag="OPU51_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_269136674.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     Typing          3070..3472
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.79"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcaaagcag tcaaggaaga accaagacag ctcactcacc agcaggctcc tttttattgg
       61 tagacacatg cttctggaca gtatcagccc ctgcacaaag tacagcagct aacaagaacc
      121 atccccagcc atcaatacca cgaaaagcta ggactccagc aatagttacg ctaatcaacg
      181 ggaacaataa aaccataaag gatctcatac tttctccaga ggttactttt ttaataaagt
      241 ccctaatcca tccgtacttt cacttggatg gattagcaac ttacttattg ataactaatg
      301 ataatcttca agatcaacgt ctaagacgtt tggttcctca accttgaaaa ctacccgcca
      361 gttcccatta acttcaacgg aatactcgcc tttacgctcc ccctttaggg ggtggcaccc
      421 taacgcccta acgtcaacaa cgtcttgaat gcaagttgct gtgcttagcg tagtcaaaat
      481 catgcgaagt cttttcgcca tactgggctg aataccggcg gtgctacctt cttcagcaaa
      541 gctcctaagt cctttatgtt ttatgttttt tatcatctac aatccccata tttttttcgt
      601 ctatacctgt atctatattt taaaatcatt ttaattagtt ccattttgcc cccttgcttt
      661 attaaaacaa ttgacgtaag tttgttcttg aggtcaatca cctcatttga cttcaacgaa
      721 tcctaagggt aaaatactgc gccttgaaaa ggccataaaa gcccatacat acgcagtagc
      781 tttgactcca acgagtatcc taaagctgac tgcttttttt gtcaatatca tgaacttgca
      841 cattgtgcaa tgcgcatact gaaaggcagt aatttttttg tataatcaaa tacaaaacaa
      901 acagaggaag gcatgataaa tctttacgat cctgttcatc ctggtgagtt tatcagagaa
      961 atgttcatgg aaccgttcga aatacctgcc gcagagttag ccgataaact tgatgtatcc
     1021 cagtctacct taagtagact gttaaatggg aaaacagact taagctatga aatggcactt
     1081 agattgtcga aggtcgtagg ccgctctgca gagtcgtggg taaacatgca gacccaattc
     1141 agcctgtcaa aaagtcgcaa atctttagcg gcagagctgg aacacttgca tccaatgttt
     1201 ccatgaaaaa agcccgcaga acgcgggctt ttgttacgta aaacacccag cagccatcag
     1261 gaggcaacca tggtagattt ctatatctca gcaatcaaaa tggacagtgc tggtcagcac
     1321 atcgaattcg taaaagttca taaaataaac cgagatggct catggaacac tgccggctca
     1381 atcagctctc gcaaattcat cagcgagctg atcagcatgg gaaaagccag ctttatgaca
     1441 gttgtaaaga acagctctac tggtaagtgg aacctaggag ccaaagtgga agcgatgaac
     1501 gatggctaca taaccacaga tccaaacaaa actaaactcg ataacttggg aaatctgccg
     1561 aaatttgtct gatatgcaat aacatccact aagttccagc agaatcccct ccctaatgat
     1621 ttaacataaa gtcataattg tacgcaccgc taaaacggcc tcatggcgtt tctgacggga
     1681 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
     1741 ttacggcatg catgagtatg cacgcaagtg catagctcga aaattcacgc cattcagcca
     1801 gggtaagtcc tgtttccggc gttctttcca tccgaaaacg gtgaacgggg acgcaattta
     1861 ttgcgtggtc gtgagccgtt gccggcgcag ccggaaaacc cgttttcagc acgttcagga
     1921 atcgggttcg tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac
     1981 gattcaccgg cgattcgact cgtgaattga atcaagagtc accatgaaca aaaaacaccc
     2041 aaagtgatga attgaatcaa gagtcaattc atcataacca tatgataata aagcgtgatt
     2101 cacatatgaa tcgtgggcaa ttcgtcagcg attcgccata gagtcgccca cgactcggat
     2161 tcaccttaca aaaaataaaa ttgcgtaatt tacaatagat aactaattga aactaaaggt
     2221 ctatctgcaa aattttaatt ttgcgtgggg tgtgggtatt tttcgtggtg aggagtgctc
     2281 agagatctgg cgatttttgg gaaggttcag gaactccccg gaatgaccgg agaggaaaac
     2341 tgaagcatga cgcggtcagg attttttcca gaatttccac catggcgccg gggattcagt
     2401 taattttgcc tggtgatttt caagaaggag catggcctgt ttcaggctgt caatgtgcga
     2461 atccttggac aaaagtaagg tgttcaggtg ttcaatctcc tgtttcagat gacgaatcac
     2521 ctgttcactc tggtcagcag aggctgtatc acgctgttca gagacctgtc cagaatggtg
     2581 ttcacccttc ggttgctcaa agggaccaaa gacacgtaaa agctctgaag tatctaccat
     2641 ttttgcatta cgtgcaccgc tacaggtgga caggtcacct gctttaatta acctgtgcag
     2701 tgttgttcta ctcttacccg tcagtcgggc agcctcacta atagacactc tggccatcag
     2761 ttatcatcct gtaatgcatc cagtctcgag gacagtgtac ggaaaagatg ctccgcatca
     2821 tcgtggagtt tctcacaaag gtcggcctcg acatcaagat caagctcgtt aagcttctcc
     2881 agcaggagca gagactgatt ctggatgaat ttagccatgt tcagcgctgt tttctgttgt
     2941 ttcgtcataa gaggcctcag agcgtcagtg ctcagtgtag ggatgttctc ttttccgctt
     3001 cctcgctcac tcagtcgcta tgctcctgcc gttcggctgc ggcgagcggc aggagcatgc
     3061 gtataaaagc tcgagaaaac cgagcggtcg cgtgacgggg aaaaggaaag gccgctaagc
     3121 ggccgttttt ccataggctc cgcccccctg acaagcatca caaagctgac gctcaaatca
     3181 gtggtggcga aacccgacag gactataaag atatcaggcg tttccccctg atggctccct
     3241 cgtgcgctct cctgttcctg ccattcggtt taccggtgtc attccgctgt tatggccgcg
     3301 tttatctcat tccacgcctg acactcagtt ccgggaaggc agttcgctcc aagctggact
     3361 gtatgcacga accccccgtt cagtccgact gctgcgcctt atccggtaac tatcgtcttg
     3421 agtccaaccc ggtaagacac gcacaagcgc cactggcagc agccactggt aagaaggtgt
     3481 acaagagatt actgatacag agttcttgaa gtctcagccc aactacggct aaactgaaag
     3541 gacagaattt ggtttcagcg gtcttgtaca ccctgttacc acggttcaga agttccccaa
     3601 cttactgaac cctcgaaaaa accgcctccc caggcggttt tttcgtctcg gcgagtaaga
     3661 tacgctttat ca
//
