LOCUS       NZ_CP114311             3851 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Klebsiella oxytoca strain 2022CK-00499 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP114311
VERSION     NZ_CP114311.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31152639
            Assembly: GCF_025579125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00499
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 3851)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 04:34:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,912
            CDSs (total)                      :: 5,796
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 62 of 129
            Pseudo Genes (incomplete)         :: 78 of 129
            Pseudo Genes (internal stop)      :: 28 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114311.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00499"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022-04-21"
     gene            complement(64..279)
                     /locus_tag="OEE41_RS29490"
                     /old_locus_tag="OEE41_29485"
     CDS             complement(64..279)
                     /locus_tag="OEE41_RS29490"
                     /old_locus_tag="OEE41_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(286..783)
                     /locus_tag="OEE41_RS29495"
                     /old_locus_tag="OEE41_29490"
     CDS             complement(286..783)
                     /locus_tag="OEE41_RS29495"
                     /old_locus_tag="OEE41_29490"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_040229893.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRKVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<824..1522)
                     /locus_tag="OEE41_RS29570"
                     /pseudo=""
     CDS             complement(<824..1522)
                     /locus_tag="OEE41_RS29570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1461..1802)
                     /locus_tag="OEE41_RS29505"
                     /old_locus_tag="OEE41_29500"
     CDS             complement(1461..1802)
                     /locus_tag="OEE41_RS29505"
                     /old_locus_tag="OEE41_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2424..2558
                     /locus_tag="OEE41_RS29575"
     CDS             2424..2558
                     /locus_tag="OEE41_RS29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072042466.1"
                     /translation="MHEPPVQSDRCALSGNYHLDSNPDGHEKTPLAAATGNKGVQDGI"
                     /name="replication initiation protein"
     gene            3013..3249
                     /locus_tag="OEE41_RS29510"
                     /old_locus_tag="OEE41_29505"
                     /pseudo=""
     CDS             3013..3249
                     /locus_tag="OEE41_RS29510"
                     /old_locus_tag="OEE41_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885913.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /name="YgdI/YgdR family lipoprotein"
     gene            3263..3490
                     /locus_tag="OEE41_RS29515"
                     /old_locus_tag="OEE41_29510"
     CDS             3263..3490
                     /locus_tag="OEE41_RS29515"
                     /old_locus_tag="OEE41_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_040229896.1"
                     /translation="MTKVIYSALAAIIALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DAGLIGYTDANGVKQQINKQEVKEVSEIPN"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(1883..2070)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.368"
                     /coverage="87.0"
                     /evalue="5.789999999999997e-54"
     Typing          2050..2534
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.593"
                     /coverage="93.0"
                     /evalue="0.0"
     Typing          complement(3..1471)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.184"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1468..1781)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.78e-58"
ORIGIN
        1 gatctgctgt cttctctggt tccttttccg ttgtttgccc gtatcgctgt tgctcaatct
       61 cattcagcct gtcgataacc ggcgcagctg ctcgctcaaa ctctgcacct gcctgctcaa
      121 gcccgacacg cgctcgctca gcgccgcgtt ctcccgttgc ataagaccag acatcttccg
      181 ccattcctcg aaggcgctct cccactcgtc cagccttctc gagtagtcct gctgtagctg
      241 ctctaacgcg ctcagcaatt gtttttccag ttccgtcatt ctgctctact cccgtaccag
      301 cttcaccgcg ttccgcgtct tgttgtccac cttccggccc gtttcggctt tcatcccctc
      361 cggcagcacc agaaaccgtc cgttgcggtt ttccagatag gtcacgcccc acgtctgcat
      421 ctgcagtttc gacagcgtat ctctctgctg cctgatttcc gccaggttcg ccgctatccg
      481 gttcccctgg taccacagca ccccgctcag gctggcgaac agcagccctg acaccatggt
      541 gatggtcagc cacgtccgcc cgaccattcg cagcacgctc agccggttcg actgcatcgc
      601 cgcgttcatc ccctgttcgt gggcgctgat ggcgtcgttg attctcttcg cgcttgcgtt
      661 cagttccgat ttgacaacgt tctcgtgctt gctgaatgcg gctttcagcc tctcttcggt
      721 aagctgctgc tgtgctttcg atttctcctc gaagtccctc gaggccgtta aaagactgct
      781 cataaatggc tcccctcagt cggatatttc gccccccgtc cgggtcggca atgctgatac
      841 tgttttttgt ggttctgacc acctcaaaac cggccgctgt gagcgcccct gtgacgtcat
      901 cacgcgtttt cagctctcct gatgaggcta acatcagcaa accccgtgta atcgtctctg
      961 cggcttcctg cttcgtttct ggcagtcctg acggcgtgac cagcgcccgc cggttctccg
     1021 ggtcgttcgg atcatgtagc cccagcctgc cgttgaccac tgtctgccac gcatcgatgc
     1081 gcggccggtc ggcacgatcg taatacggct ggaggcgttt ccccgtcagt aattcggtat
     1141 tggggatcag aaaattcagc tcaagacgac ccttgtctgc gtgttccacc cacagaatgc
     1201 tgtactgatc tttatcgagt ccgggcatca gaacccgctc aaagctggcc atcagttttt
     1261 cacgctgacc cggcggcaga tccgcctccg cgaatgacag caccccggag gtatattttt
     1321 tcacatacgg cgacgcatca atcagctccc ggatttcttc cggcttcccc tgcaggacgc
     1381 tggcaccttc acgctgccgg tctttaccca gaagatagtc caccggaccg gcgcccccgc
     1441 cgcgaccccg tggatggaat ttaacaatca tcatccggcc ctttttccag tacggcatgc
     1501 cgcaaccgtt ccagaccggc atcgatcgcc atcagcgctg ccacgatctg cacccggtca
     1561 tgaccggcac caccggcgtt cactttccgg gcgatctggt tcaggttatt gcccatgccg
     1621 gcaagctgac gcagcagcgc cggcgaaatc gagggaagcc tgccggcgcg tgtcggtttc
     1681 tcatcaaggc aggtctggcg catccacgcc gccagttgtt ttccgtcgca ccgctcaagc
     1741 aaccgccgat gttcgtcttc cgtcacccac atcgtgagca tcttgttgcg tttatctgcc
     1801 agaatcgcct cctgataacg ccgtggcgga cgggaaagcc tttcccggtc ggcgcggggt
     1861 tggacaagcc gcaggcgcgt cagtggtttt tagcgggttt cggggcgcag ccctgaacca
     1921 gtcacgtagc gctagcggag tgtatactgg cttaatatga taaatcgaag tggtaattcg
     1981 ggaaagtgct tcatgtggca ggggaaaaat tcagctgcgg ggcgtaagtg caaaatgcag
     2041 gtggtgatga catgacgcct cctcgctcac tcggtcgctg cgctcctgcc gtgagacggc
     2101 ggcgggcgtt acctgctcac gaatgacaag atagagagac tgcgctgaaa tcaggaaggg
     2161 ctggagaaag gatgcggcgt tgccgttttt ccataggctc cgcccccctg acaagcatca
     2221 caaaatctga cgctcaaatc agtggcggcg aaacccgaca ggactataaa gatcccaggc
     2281 gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     2341 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     2401 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     2461 tatccggtaa ctatcatctt gattccaacc cggacggaca cgaaaaaacg ccactggcag
     2521 cagccactgg taacaagggt gtacaagatg gtatttaaag aggtcttgaa gtggatgcct
     2581 gactgcggct acactgaaag aacagttttg gtaattgcgg tcttgtacac ccagttacct
     2641 cggttagaga atctgccagg atcctctatc catcgaaaaa gccaccggaa ggtggttttt
     2701 tcgttttcag agcaagagat tacgcgcaga aaaaaatgat ctcaagaaga tcctttcttc
     2761 gcaggatcga tatccatggg atttatgatg ttccttgtaa tgaatccatc actggaatgc
     2821 atttatcggc ggaagaaatc cccctgcgtt acaaaatgat tatgattagc aactagccgt
     2881 ttaacacagc attctgccac ctgatttaac ataagatttc tttttttaca ttttatctcc
     2941 ccttccgatg cagaagccaa aacaatgcct attattagta agttagatac taacttcgcc
     3001 agtactaggg atatgggata tctaaaaaag cattggtttc tcttaataac ttcagtttta
     3061 accttctcag taaccggcac ctcctaaaat caggccatta aaactaccga cggacggact
     3121 atcgtcgcag acggtaagcc tcagattgat agtgacaccg ggctggtttc ttacaaggat
     3181 gcacagacag gaaaaacaga acatatcaac cgcgacaaaa ttaccaacat gagtgaacta
     3241 gataactgat aaggaacgtt ctatgacgaa agtaatttat tcagcgctag ctgcgatcat
     3301 tgcactttca gctctgacag gatgcacaag gacaagctat gcaattcata cgaatgatgg
     3361 ccgtaccatt gtgagcgatg gcaaacccaa agaatcggat gctgggttga ttggctatac
     3421 cgatgctaac ggcgtgaagc agcaaattaa taaacaggaa gtcaaagaag tctcagagat
     3481 tcctaactga tcataaaaaa ccgcctagtg gcggtttttt tttacatgct agtaaccgtc
     3541 caattaaaca taacatccct tactcgcacc gcgccggagg cactcagggc gtttctgatg
     3601 gctgacgggc aaaaggtggt ggcaagtaca ccgaaaacca ccagaaacgc acctgattta
     3661 ttttacgcat atgcatcact tattcacatc actgcatagc cggtgatttc ggttaaaaaa
     3721 gactctctgt gtgctgaaat cgcagaaaat ggccatgatc cccgtcacgc tcaggccacc
     3781 tgagacgggc gtttatcagt ctccatactc cctgtcacca tgagataatg tgcgttgccg
     3841 ttccggtacc a
//
