LOCUS       NZ_CP114155             2496 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella michiganensis strain 2022CK-00496 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP114155
VERSION     NZ_CP114155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31152637
            Assembly: GCF_027577885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00496
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:56:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,048
            CDSs (total)                      :: 5,930
            Genes (coding)                    :: 5,774
            CDSs (with protein)               :: 5,774
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 61 of 156
            Pseudo Genes (incomplete)         :: 107 of 156
            Pseudo Genes (internal stop)      :: 32 of 156
            Pseudo Genes (multiple problems)  :: 37 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114155.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00496"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2022-08-08"
     gene            join(2441..2496,1..130)
                     /locus_tag="OEE45_RS30185"
                     /old_locus_tag="OEE45_30195"
     CDS             join(2441..2496,1..130)
                     /locus_tag="OEE45_RS30185"
                     /old_locus_tag="OEE45_30195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(421..612)
                     /locus_tag="OEE45_RS30190"
                     /old_locus_tag="OEE45_30200"
     CDS             complement(421..612)
                     /locus_tag="OEE45_RS30190"
                     /old_locus_tag="OEE45_30200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            1848..2075
                     /locus_tag="OEE45_RS30195"
                     /old_locus_tag="OEE45_30205"
     CDS             1848..2075
                     /locus_tag="OEE45_RS30195"
                     /old_locus_tag="OEE45_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(178..265)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          905..1433
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 acttctcctc cttgagcggc tcgagcagat ggatctggat gaggctgccg gctgctgtga
       61 gcacctgcac gatcaggccg aagcgcttta cgccatgctg aacgcacaga taggcgagga
      121 aaatgcgtga aaatcggtga acgggtgcgc aattcagtgc gcggccgtga ggcgatggcg
      181 gggtgtcggg gcgcagccct gaccagggta tttgtgatgc cggcgcgtgc gcggtattac
      241 aaatgcacat cctgtcccgg aacggacacc gggaaacagc aaaaaaaacc gggcggcacg
      301 cccggaactc aatcaagtta gattagatta ctctcactcg tccataacag catcatggaa
      361 cgacgaccac cgtccgtgac ggccgcctcg tttaagtatg gacagaaata cagaaaatgc
      421 tcaggacgaa atgtaatgaa tgcgaacgga ttcaagaaat tcgagcatga cagtccttac
      481 ggccggttcg gtttcagaca aaatctgccg gtatgcatcc agcatcatgg ctccggcatc
      541 ccctccggca cgccgtagcc agaccgaaac aacggacaca agcaggtgtc gctcatcatc
      601 actaagagtc atcagggctc cggaagaaaa accaaacatg gcttacctac tgttaacggc
      661 aacgcagaag aaaggtttac agggtagcgg gcagggatga aaaaaaaaaa aattgcagtc
      721 aggccggaac ggctgctgaa ccggtacagg cgggttccgg gcgcagcgcc ggaccagtca
      781 cggagcacta gcggagtgta tactggctta agatgtagca tggcagtaac acatagcatt
      841 aatgccacat aagacaggcg cagaaagtga aagaaaacgc gtcaggagaa aatgtgatac
      901 agaatatatt ccgcttcctc gctcactgac tcgctccgct cggtcgttcg gctgcggcga
      961 gcgggacggg cttacggatg gatcagaaat ttcctggggg atgccaggaa gatacttaac
     1021 gaagaaagca taaggccgcg ccaaagccgt ttttccatag gctccgcccc cctgacaagc
     1081 atcacagaat ctgacgctca aatcagtggt ggcgaaaccc gatgcggctt aaagatacca
     1141 ggcgtttccc cctggtggct ccctcgtgct cccccgcatt cctgcctttc ggtttaccgg
     1201 tgtcactccg ctgttatggc cgcggtttct cattccacgc ctgacactca gttccgggaa
     1261 ggcagttcgc tccaggatgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     1321 cttatccggt aactatcaac ttgagtccaa cccggaaaga cacgactaat cgccattggc
     1381 aacggccatt ggcaaacagg atgtgcgaga gatgtctcaa gagttcttga agtggcggcc
     1441 ttagtgaggc tacactgaaa ggacaaattt ggtgacatgt ctcgcacaag actgttacca
     1501 cggttagggg atctgccagg attacctaac cttcgaaaaa ccacctgcca gggtggtttt
     1561 ttcgtttaca gggcaagaga ttacgcgcgg aaaaaaagga tctcaagaag atcctttata
     1621 aattagacat ccaacctcac aaagaggaat acttaaatca tcttggtata tgtatcggcc
     1681 ggattacatc tcatataagc ctcctccccg cctgacacca cactttgacc atagcacctc
     1741 gcgcctaact cgatatacag cccaccatca agcctctttt cacataaaaa atgacctata
     1801 gttagatggt tagtgaccaa aactgatatg gataaaagga gctggctttg aaaaagattt
     1861 ttgtttcagt gttcgcagcg gcagttgcgc tttcagccct caccggctgc acgcgcacaa
     1921 gctatgccat acacaccaat gacggtcgca ctattgtcag cgacggcaaa cctaaagaat
     1981 cagactcagg cctgcttggc tatacagacg cgaatggtgt caaacagcag atcaataaaa
     2041 ccgacgttaa agaagtctcc gaaattccgc actgagcatc ttctccgaaa aaggccgcta
     2101 atgtggcctt tttctttacc tgtggttctc cgccaaaatc ccagcaaatt gcatcaccaa
     2161 agctaaaagc tttcagggtt gtctaaaaag cacaagacat aagaggaagt gcggtatttt
     2221 ataatcaagc ccccaagaat ttaccataac atccgttgcc cgcaccgcct gagacgcgtt
     2281 cagcgcgttc ctgacgaaac catgacaaaa accacaacaa accaccccgg aacccgtcag
     2341 aaacgcgcct gttaaatttt aacggcatgc atgactatgc accagaatga cgccatgctc
     2401 ttttcacgca aaaatcatca ccagacgggg aaaatcacca gtgaccagac aggaatccgc
     2461 cgccctcaat atggccaaat ttatccgcgc acagac
//
