LOCUS       NZ_CP114154             4307 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella michiganensis strain 2022CK-00496 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP114154
VERSION     NZ_CP114154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31152637
            Assembly: GCF_027577885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00496
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:56:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,048
            CDSs (total)                      :: 5,930
            Genes (coding)                    :: 5,774
            CDSs (with protein)               :: 5,774
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 61 of 156
            Pseudo Genes (incomplete)         :: 107 of 156
            Pseudo Genes (internal stop)      :: 32 of 156
            Pseudo Genes (multiple problems)  :: 37 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114154.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00496"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2022-08-08"
     gene            14..337
                     /locus_tag="OEE45_RS30150"
                     /old_locus_tag="OEE45_30160"
     CDS             14..337
                     /locus_tag="OEE45_RS30150"
                     /old_locus_tag="OEE45_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            546..>980
                     /locus_tag="OEE45_RS30155"
                     /old_locus_tag="OEE45_30165"
                     /pseudo=""
     CDS             546..>980
                     /locus_tag="OEE45_RS30155"
                     /old_locus_tag="OEE45_30165"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1015..1506
                     /locus_tag="OEE45_RS30160"
                     /old_locus_tag="OEE45_30170"
     CDS             1015..1506
                     /locus_tag="OEE45_RS30160"
                     /old_locus_tag="OEE45_30170"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064183955.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRLSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1513..1728
                     /locus_tag="OEE45_RS30165"
                     /old_locus_tag="OEE45_30175"
     CDS             1513..1728
                     /locus_tag="OEE45_RS30165"
                     /old_locus_tag="OEE45_30175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064183954.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2205..2648)
                     /locus_tag="OEE45_RS30170"
                     /old_locus_tag="OEE45_30180"
     CDS             complement(2205..2648)
                     /locus_tag="OEE45_RS30170"
                     /old_locus_tag="OEE45_30180"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_064183953.1"
                     /translation="MIAVSLPDELLKRLDDAVDKTGKKRSFLIRESISMYLNNIENIHE
                     KKEIILNTSKPFYEVLIEEFKNEKKLITEARKTEFTMFSDNGKLYVINSKGNTRRLEPM
                     YVNNFFEEYKKTGSMSTSSYQDITFNSSYLLAALQYLMEKELI"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            complement(2900..3337)
                     /locus_tag="OEE45_RS30175"
                     /old_locus_tag="OEE45_30185"
     CDS             complement(2900..3337)
                     /locus_tag="OEE45_RS30175"
                     /old_locus_tag="OEE45_30185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064183952.1"
                     /translation="MQQAIRMRIEADKAALDAGFTTNNFVGELGEKLAHAVYGGRILPN
                     SAKSADIQTDDGTLLQVKTRKPNKGKQFGSIRSWDFDYLIAITLGDKYEVIRALKIPVS
                     TCKELADHRAHTNSFIIKPTRKLLSHADIEDVTDKFQSVVL"
                     /name="hypothetical protein"
     gene            complement(3566..3679)
                     /locus_tag="OEE45_RS30180"
                     /old_locus_tag="OEE45_30190"
                     /pseudo=""
     CDS             complement(3566..3679)
                     /locus_tag="OEE45_RS30180"
                     /old_locus_tag="OEE45_30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          4034..4217
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.583"
                     /coverage="86.0"
                     /evalue="2.62e-72"
     Typing          complement(3567..4048)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.86"
                     /coverage="92.0"
                     /evalue="1.6700000000000003e-157"
     Typing          15..331
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="6.01e-61"
     Typing          328..1820
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaacggaac aagatgctca ccatgtgggt gacggaggac gagcaccggc ggctgcttga
       61 gcgctgtgac ggcaggcagc tggcggcgtg gatgcggcag acctgcctgg acgaaaagcc
      121 tgcgcgcacc ggaaagctcc cctcaatctc gccggcgctg ctgcgccagc ttgccggcat
      181 gggcaataac ctgaaccaga tagcccgccg tgttaacgcc ggcggcggca ccggccatga
      241 cagggtgcag attgtggcgg cgctgatggc catcgatgcc gggcttgagc ggctgcggca
      301 cgccgttctt gagaaaggaa cggacgatga tcgttaaatt tcatccacgc ggtcgcggcg
      361 gtggcgccgg gccggtggac tatctgctgg gtaaagatcg gcagcgtgaa ggcgccagcg
      421 tcctgcaggg gaagccagaa gaagtccggg agctgattga cgcgtcgcct tacgtgaaga
      481 aatacacctc cggcgtcctg tcgtttgcgg aggccgatct gcctcccggc cagcgggaaa
      541 aactgatggc cagcttcgag cgggttctga tgcccggact tgataaagat cagtacagca
      601 ttctgtgggt ggagcacgag gacaaggggc gtctggagct gaactttctg atccccaata
      661 ccgagctgct gaccggcagg cggctccagc cgtactacga ccgcgccgac cggccgcgta
      721 tcgatgcctg gcagaccatc gtgaacggca gactggggct gcacgacccg aacgcaccgg
      781 aaaaccgccg ggcgctgatc acgccgtccg ggctgccgaa aacgacgcag gaggccgcag
      841 aagccatcac gcgcggactg ctgacccttg cttcgtccgg ggagctgaaa acccgtcagg
      901 acgtcactga ggcgctggaa cggtcaggtt ttgaggtggt gcgcaccacg aaaagcagta
      961 tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagcc atctatgagc
     1021 agtcttttaa cgctggcgaa ggacttagag cagcaatcga aagcgcagaa gcagagtacc
     1081 ggcgagatgc tgaaagccgc attcagcgag cacgagcagt ctgtcagagc ggaactgagc
     1141 gcaagcgcga ggagaatcag cgacgccatc ctcgcacacg agcagagtat gagcgaggcg
     1201 atggagaaaa accggctgag cgtactgcgg actgccgggc ggacatggct caccatcctg
     1261 atggtgtcag cgctactgat cggcacgagc gggagcattc tgtggtggca ggggcagcag
     1321 ataaccgaca attacacgca tcttcgtcag caggaagata ccctggcgaa aatgaccgcc
     1381 cggacgtggg gagtgcgcta tcaggagagc agcgacggcc ggaggtttct tatcctgccg
     1441 ccggggatgc aaaccgaagc catcccttac gacgggacga catggatccg cctgaaacag
     1501 gagtagcgga aaatgacaga gctggaaaag cagttgctga gcgcattaga gcagctacag
     1561 caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg gcggagcatg
     1621 tgtggtctta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga cttgagcacg
     1681 caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggagctgagt acggtagaac
     1741 agcggcagcg ggagcatgag tatcagaaaa gcctggagtt gcagcgccag ccgcagaaaa
     1801 cctacaaagg accgacgctg ggctgacgga tgccgtcccg tgacaaaccc caagaagaag
     1861 ggctgcgaaa ttccccgcta aatacgtcgt gatgcaaaga tcacggccta taccaggggg
     1921 aattatgaaa ctgcttaagg ttattgaagc tgtacttaac tgcctgcatc tggcggcaga
     1981 actgtgggag tggctggaga agcatttgcc ggttttcggg gccttcacgc tgtttttggg
     2041 ctgaatcctg ccgtcattca cctggctgca tggctatgca gttgggtaaa atttatctgt
     2101 cgcgatcggg gtggtttacc gggtgggttg ttgttggttt tggctgactg ccgccccgtt
     2161 cgtggcgaag ctgtccgggg cggtgcgggc aatgagtgct tatgttaaat cagttctttt
     2221 tccatcaggt actgtagtgc agctaaaaga taagatgagt tgaaggttat atcttgatag
     2281 ctagatgtac tcatacttcc tgtcttttta tactcttcaa agaaattatt cacatacatg
     2341 ggttctagcc tgcgcgtatt ccctttcgaa tttatgacat agagttttcc gttatcacta
     2401 aacatcgtga actcagtttt tctggcctca gttattagtt ttttttcatt tttaaattct
     2461 tcaatgagga cctcatagaa cggttttgag gtgttcagga tgatctcttt tttttcatgt
     2521 atgttttcta tgttattcaa atacatagat attgattctc gtatcaagaa tgagcgtttt
     2581 ttaccagttt tatctacagc atcatctagt ctttttagta actcgtctgg aagtgataca
     2641 gcaatcatga acagccctct aaatttataa tctaattaac taatctaaat ttatataaaa
     2701 atagagcggc cgtcaatagt cgagatttca ttttctaatc agttgcatgt tctgattagt
     2761 tcgtaggtac gtgcaaggga tgttatggta aaagacggta aagccggatg cttgtgcaat
     2821 agcgcccttc tactcaagat ggcggttttt ttacggtcgt gatttcgtgc agaattattc
     2881 aagtaggggg taaaaccacc tataaaacaa cactctgaaa tttatccgtc acatcttcaa
     2941 tatccgcatg tgaaagtaat tttctggtcg gctttattat gaaagagttc gtatgtgccc
     3001 gatggtcggc cagttcttta caggtagata ctgggatttt taaggccctg atgacttcgt
     3061 atttatcacc cagcgttatc gctatcagat aatcaaaatc ccaagagcgg atgctaccaa
     3121 actgttttcc tttgttcggc ttcctcgttt tcacctggag taaagtgccg tcgtctgtct
     3181 gtatgtctgc gcttttagct gagttaggaa gtatgcgacc tccatatacg gcatgagcta
     3241 atttttcgcc cagttcaccg acaaaattat tcgtagtaaa accagcatct aatgctgctt
     3301 tatcggcttc gattctcata cggatggctt gttgcatact ttttgtagcc cttaatgggg
     3361 gaagatcttc ttgagttcct ttttttctgc gcgtaatctc ttgctttgaa aacgaaaaaa
     3421 ccgcccagca aggcggtttt tcgaaggttc tcagagcagc aactctttga accaaggtaa
     3481 ctggcttgga ggagcgcaga taccaaaact gtcctttcag tgtagcctta tcgcacccga
     3541 aacttcaaga ctctcctcct ctaaatcagt taccaatggc taccgccact ggcgattttt
     3601 tgtgtctgta agggttggac tcaagacgat agttacctta catggcgcgg tagtcggact
     3661 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     3721 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     3781 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
     3841 cctgtcgggt ttcgccacca ctgatttgag cgtcatattt cgtgatgctt gtcagggggg
     3901 cggagcctat ggaaaaacgg caacgccgca tcctttttcc ggcccttcct gacttgagta
     3961 ctgcttgcgt gtcccgtcat ctttgagctg gtaacgcccg ccgcagtctc acggtaggag
     4021 cgtagcgacc gagtgagcga ggaggcgtaa tttcatcctt atctgcatgt tgcacttacg
     4081 cccggtagcc tcatttttcc ccagccacat gaagcacttc catacattac cactccgccg
     4141 taacatatta agccagtata cactccgcta gcgctacgtg actggttcag ggctgcgccc
     4201 cgaaacccgc taaaaccact gacgcgcctg cggcttgtcc aacaccgcgc cgaccgggaa
     4261 aggctttccc gcccgtcccg gcgttatcag gaggccggtt tgtcaga
//
