LOCUS       NZ_CP114153             5524 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella michiganensis strain 2022CK-00496 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP114153
VERSION     NZ_CP114153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31152637
            Assembly: GCF_027577885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00496
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:56:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,048
            CDSs (total)                      :: 5,930
            Genes (coding)                    :: 5,774
            CDSs (with protein)               :: 5,774
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 61 of 156
            Pseudo Genes (incomplete)         :: 107 of 156
            Pseudo Genes (internal stop)      :: 32 of 156
            Pseudo Genes (multiple problems)  :: 37 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114153.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00496"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2022-08-08"
     gene            104..388
                     /locus_tag="OEE45_RS30120"
                     /old_locus_tag="OEE45_30130"
     CDS             104..388
                     /locus_tag="OEE45_RS30120"
                     /old_locus_tag="OEE45_30130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_256657193.1"
                     /translation="MVDTSELLRVFGPFEQPKGEHHSGLVSEQRDTASADHSEQVIRHL
                     KQEIEHLNTLLLSKDSHIDSLKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            1434..2186
                     /locus_tag="OEE45_RS30125"
                     /old_locus_tag="OEE45_30135"
     CDS             1434..2186
                     /locus_tag="OEE45_RS30125"
                     /old_locus_tag="OEE45_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023307481.1"
                     /translation="MGIENSNQGPGWHDHDTPYGTIHSYDGDTGTYGGGGTGGNAGGGH
                     TGLTANTWVDIATTKDYNGRYMSMCLNNLKDHEGSINTMYRDSKGNITVGIGHYLGSAE
                     MAQALPFNRTKVMHVHGDDLESEVAVSKSDIASAFNAYKSNPRDVPSMHLSNADVIGQC
                     IKDVQTTENGLRGLYPGYDRFSDPRKTALVDMGFNLGIPTLKSKFPKFNDAVNRGDWNV
                     AADESHRDLNQRGDKRNKDTADQLRYNR"
                     /name="hypothetical protein"
     gene            2288..2788
                     /locus_tag="OEE45_RS30130"
                     /old_locus_tag="OEE45_30140"
     CDS             2288..2788
                     /locus_tag="OEE45_RS30130"
                     /old_locus_tag="OEE45_30140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023307482.1"
                     /translation="MIRFVIAAFISILTIKGAFADINVFIAPNDLNYETNSTSEINHYL
                     DELKKNNTKVDVDKDPKISNELKKYIGIVNDRVSDVFSESEFKKYRGDVCKISITTGDT
                     APSDPVLTQVFTYDELADNKKQSCQKLNNELIKKVAKDKGLSYPKVVKDYGSLSIDMIF
                     LFK"
                     /name="hypothetical protein"
     gene            2836..2970
                     /locus_tag="OEE45_RS30135"
                     /old_locus_tag="OEE45_30145"
     CDS             2836..2970
                     /locus_tag="OEE45_RS30135"
                     /old_locus_tag="OEE45_30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_110216848.1"
                     /translation="MSICVIILLAACQANYIRDVKGGTVAPSASSDIRTSSSQPIGSS"
                     /name="colicin release lysis protein"
     gene            complement(3135..3277)
                     /locus_tag="OEE45_RS30260"
                     /pseudo=""
     CDS             complement(3135..3277)
                     /locus_tag="OEE45_RS30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3693..3884
                     /locus_tag="OEE45_RS30140"
                     /old_locus_tag="OEE45_30150"
     CDS             3693..3884
                     /locus_tag="OEE45_RS30140"
                     /old_locus_tag="OEE45_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4211..5233
                     /locus_tag="OEE45_RS30145"
                     /old_locus_tag="OEE45_30155"
     CDS             4211..5233
                     /locus_tag="OEE45_RS30145"
                     /old_locus_tag="OEE45_30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_000156887.1"
                     /translation="MTITTVGIDLAKNVFAVHGVDQNGRTVLVKPKVSRAALPELIASL
                     PPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICEAV
                     RRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSADALRH
                     MVSVQKSSLPLQVQQCVDDLMEHVDRIEANITEYDRILSHIAKTDHRSQRLMELKGVGP
                     TTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQG
                     ARSVLIGAGKRTDSFSRWVCMLVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSVN
                     "
                     /name="IS110 family transposase"
     Typing          complement(2958..3459)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="99.0"
                     /evalue="1.02e-154"
ORIGIN
        1 tattagtgag gctgcccgac tgacgggtaa gagtagaaca acactgcaca ggttaattaa
       61 agcaggtgac ctgtccacct gtagcggtga acgcaatgca aaaatggtag atacttcaga
      121 gcttttacgt gtctttggtc cctttgagca accgaagggt gaacaccatt ctggactggt
      181 gtctgaacag cgtgatacag cctctgctga ccatagtgaa caggtgattc gtcatctgaa
      241 acaggagatt gaacacctga ataccttact tttgtccaag gattcgcaca ttgacagcct
      301 gaaacaggcc atgctccttc ttgaaaatca ccaggcaaaa ttaactgaat ccccggcgcc
      361 atggtggaaa ttctggaaaa aatcctgacc ccgtcgtgct tcagttttcc tctccggcca
      421 tcccggggag ttcctgaacc ttcccaaaaa tcgccagatc tctgaacgct cttcaccact
      481 aaaaataccc acacactacg caaaattaaa attttgcaga tttgtattta atatcagtag
      541 tttactttca tgaaatttcg ttttttaaat tttatttaca tagatccgac tctatgacga
      601 ctcgtcatag agtccccggc gagtcgtcat agagtcggta acgactcagg actataaaaa
      661 caatgagtta ataacgaccc gactctagac ccgactcccc gtttggttgt tttttagacg
      721 cggcaactct gggtacgact ctagtgagga gtcgccggtg aatcgtgtgg tgagtcgtcc
      781 ggcgacttgc cataacgtga ttgccggccg tcgacgaacc cgattcctga acgtgctgaa
      841 aactggtttt ccggctgcgc cggcaacgct tcacggccac gcaataaatt gcgtccccgt
      901 tcaccgtttt cggatgggaa gaacgccgga aacaggactt accctggctg aatggcgtga
      961 attttctcgc tatgcacttg cgtgcatact catgcatgcc ttaaaaacag agcctgcgcg
     1021 tttctggcgg gttttcgggt ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc
     1081 ctgaggccgt tttagcggtg cgtacaatta tggctttatg ttaaatcatt gccgcaaaca
     1141 gccacgaaaa gcttacttgg catcgtctct actagcctgt taaaactcat attttgaact
     1201 caatcattta aatttcgtct tttcaggcgt acaacttcga taactaaaaa tttatgtgaa
     1261 atagatattt atccaacagt tatgtagata agaataaaac atcaatattt ttttgagagt
     1321 gtcaaggaag ttatagtgag aatttttcta aaagtattga cagattcaaa gaagatgaat
     1381 aaattgatac tgtttattta tacagttgtt tttttgtcaa agaggtaatg tttatgggta
     1441 ttgaaaattc aaatcagggg ccaggatggc atgatcatga tactccttat ggcactattc
     1501 acagctatga tggtgatact ggtacgtatg gaggtggtgg aactggaggg aatgctggtg
     1561 gtgggcatac cggtcttact gcaaatacat gggtagatat cgcgacaaca aaagattata
     1621 acggtagata tatgtctatg tgtttaaata atcttaaaga ccatgaaggt tcaattaata
     1681 caatgtatag agactctaaa ggtaatatta ccgttggtat tggtcattac cttggttctg
     1741 ctgagatggc tcaggcatta ccttttaatc gaacaaaagt tatgcatgtg catggtgatg
     1801 atcttgaaag tgaggtcgcc gtatctaagt ctgatatagc ttcagcattt aatgcataca
     1861 aatcaaaccc aagagatgtt cctagtatgc atctttcaaa tgctgatgta ataggtcagt
     1921 gtattaagga tgttcaaact actgaaaatg gtttgcgtgg actttaccca ggttatgatc
     1981 gtttttcaga tccacgaaaa acagcattgg tagatatggg ttttaatctt ggtattccta
     2041 cgcttaagtc taaatttccg aagtttaacg atgcagttaa tcgtggtgat tggaatgttg
     2101 ctgcagatga aagtcatcga gatcttaatc aaagaggtga taagcgtaat aaagatacgg
     2161 cagatcaatt gcgttataat cggtaatgat ttagatttac aactcctgcc atcaaatggc
     2221 aggagtgtcc tcacgatatt aaaactaagt catgaggttt ctaatcttat attttatggg
     2281 tggtattatg attcgttttg tgattgctgc ttttatttca atcttgacaa ttaaaggagc
     2341 ttttgctgat atcaatgttt ttatagcgcc taacgatcta aattatgaaa caaattctac
     2401 tagtgaaatt aatcattatt tagatgagct gaaaaaaaat aatactaaag ttgatgttga
     2461 taaagaccca aaaattagca atgagttaaa aaaatatatt ggtattgtta acgatagagt
     2521 gtctgatgtt ttcagtgaaa gtgaatttaa gaagtatcgt ggcgatgtct gtaaaatatc
     2581 aataacaact ggggatacgg ctccatctga tcctgttttg actcaagtgt ttacttatga
     2641 tgaactagct gataataaaa aacaatcatg tcaaaaatta aataatgagc taataaaaaa
     2701 agttgctaaa gataagggtt tatcatatcc gaaagttgtt aaagattatg gatctttatc
     2761 aattgatatg atctttttat ttaaataaaa ataaatttac ggtgttgttt atgcctttta
     2821 aaaaagtctt ttccaatttc tatttgtgta attatattgt tggctgcctg ccaagctaac
     2881 tatattaggg acgtaaaagg aggaactgtt gctccatcag cttcttcaga tataagaacg
     2941 tcgagttcgc aacccatagg atcttcttga gatccttagc ggatagtcgt aatctctgct
     3001 ctgtaaacga aaaaaccacc cggcaaggtg gttttttcga aggttcatta accggcaagt
     3061 ttctgaaccg tggtaactgg ctttcgcaga gcacagaaac cgaattagtt ctaacagtgt
     3121 agctgtagtt tacctcacac ttcaacactt ctttgttctg cgaatgcgtt accagtggct
     3181 gctgccagtg gtgataagtc gtgtcttttc gggttggact caagttgata gttaccgaaa
     3241 aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     3301 cctacccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     3361 acaccggtaa accgaatggc aggaacagga gagcgcacga gggagccatc agggggaaac
     3421 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagattccg
     3481 tgatgctcgt caggggggcg gagcctatgg aaaaacggcc gccttgcggc ctttccttcc
     3541 tctcctccgt tgaacgctct gctttctcga gctgttatac ggatgctcct gccgctcgcc
     3601 gcagccgaac ggcggaagcg tagcgactga gtgagcgagg aagcggaaaa gagaacatcc
     3661 ctatactgag cactgacgct ctgaggcctc ttatgacgaa acaacagaaa acagcgctga
     3721 acatggctaa attcatccag aatcagtctc tgctcttgct ggagaagctt aacgagcttg
     3781 atctcgatgt tgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttcc
     3841 gtacactgtc ctcgagactg gatgcattac aggatggtaa ctgatggcca gagtgtctat
     3901 taggggtcag tttggagatc ggaaattttt gtacgttaag cgcattattt aaccaattga
     3961 ggttcgtaac ggattgattt tgactcagta gctatcgctt ttaagttgtc gctcttgcgg
     4021 tattgcattt aggttatctg attgaggtca aatcccctga aaacggcgta tagcgcggga
     4081 gtacacccta ttgctatagg taagtctgtt caaaaaacag gcttaccgaa tggaagggac
     4141 ttcccctgat tatgcgcctg attaactctt gtgcaatcat ggggtacaca tcgtcccgga
     4201 ggaattcgtt atgactatca cgactgtcgg tatcgatctt gctaaaaatg tgtttgctgt
     4261 tcacggtgtt gatcaaaatg gtagaactgt tctggttaag cctaaagtat cgcgtgctgc
     4321 acttcctgag ttaattgcaa gtttgcctcc ctgtgttatc gggatggaag catgctccgg
     4381 ggctcactac tgggcaagat tgtttcggca gtatggtcat gagccacgtc tgatggctgc
     4441 taagtttgta tcgccttatc gtatggccgg aaaatcagga aaaaatgatg cagctgatgc
     4501 tcaggctatc tgtgaagctg ttcgtcgtcc gcatatgcgg ttcgtaccag tgaaagacga
     4561 aagccagcag gccatgcagt gtttacatcg tacccgtcag ggttttatcg aagagaaaac
     4621 agcaacgtat aatcgtctgc gaggattgat atctgaattc ggtgtcatcg cgccacaaag
     4681 tgctgatgcc ctgcgccaca tggtttctgt gcagaagagc tctttaccgc ttcaggttca
     4741 gcaatgtgtt gatgatttga tggaacacgt tgatcgcatt gaagctaaca tcactgaata
     4801 tgaccgaatt ttgtcccaca tagccaaaac agatcatcgc agtcagcggc tgatggagct
     4861 aaagggcgtt ggtcccacaa cggcctgtgc actggtcgcc agtatcggta atgcacatga
     4921 ttttaaaaat ggacgtcaac tggcagcctg gctggggctg acgccatcac aatacagcag
     4981 cgggggaaag tcgaaactcg gcaggatcac aaaagccggt gattcgtatc tgcgaacgct
     5041 tctggttcag ggggctcgtt cagtgcttat tggcgccggg aaaaggactg actcattcag
     5101 ccgttgggtt tgtatgttgg ttgagcgcag agggtactgg cgggctgttg tggccatcgc
     5161 cgccaaaaat gcgcggctgt gttgggcatc actacattac ggtgatgatt tccggctgta
     5221 ctcagtcaac taaagctcga agctgtataa ccatctgacc tgcaattcgt tgatgataaa
     5281 gggttagacc ccgtgaggcc tatctggcta ttgtacagga tatacgtatc cgcttaacga
     5341 acaagaacct cacgcgcgtc tttcatcagg gcccgaatcg atgacgattc atcatggccg
     5401 tttatagtac cgcagtctct cccctttatt tttactgaag agcagacaga aaccataaat
     5461 accggctttg acatgccggg gaagtcctta tagtacaata attctctata tccaaactga
     5521 cccc
//
