LOCUS       NZ_CP113908             8300 bp    DNA     circular CON 02-APR-2024
DEFINITION  Klebsiella michiganensis strain PS_Koxy4 plasmid pPSKoxy4_4,
            complete sequence.
ACCESSION   NZ_CP113908
VERSION     NZ_CP113908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12648330
            Assembly: GCF_014050515.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Shibu,P., McCuaig,F., McCartney,A.L., Kujawska,M., Hall,L.J. and
            Hoyles,L.
  TITLE     Improved molecular characterization of the Klebsiella oxytoca
            complex reveals the prevalence of the kleboxymycin biosynthetic gene
            cluster
  JOURNAL   Microb Genom 7 (6) (2021)
   PUBMED   34142942
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Hoyles,L.
  TITLE     Phage vB_KmiS-Kmi2C represents a novel genus of lytic bacteriophage
            that infects members of the Klebsiella oxytoca complex
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8300)
  AUTHORS   Hoyles,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2022) Biosciences, Nottingham Trent University,
            Clifton Lane, Nottingham NG11 8NS, United Kingdom
REFERENCE   4  (bases 1 to 8300)
  AUTHORS   Shibu,P., Mccartney,A.L., Kujawska,M., Hall,L.J. and Hoyles,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2019) Biosciences, Nottingham Trent University,
            Clifton Lane, Nottingham NG11 8NS, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: PS_Koxy4_hybrid
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014050515.2-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 06:27:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,156
            CDSs (total)                      :: 6,040
            Genes (coding)                    :: 5,888
            CDSs (with protein)               :: 5,888
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 47 of 152
            Pseudo Genes (incomplete)         :: 119 of 152
            Pseudo Genes (internal stop)      :: 19 of 152
            Pseudo Genes (multiple problems)  :: 31 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113908.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="PS_Koxy4"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pPSKoxy4_4"
                     /geo_loc_name="United Kingdom: London"
                     /collection_date="2015-09"
                     /identified_by="P. Shibu"
     gene            1..882
                     /locus_tag="F0A14_RS30645"
                     /old_locus_tag="F0A14_030640"
     CDS             1..882
                     /locus_tag="F0A14_RS30645"
                     /old_locus_tag="F0A14_030640"
                     /function="plasmid replication"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_014454108.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIADN
                     LTLEPIMGKRLNVMDERHLARVVEFCADARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLLPVELLEVPNGKKA
                     GSFQKGGDDERW"
                     /name="helicase RepA family protein"
     gene            869..1615
                     /locus_tag="F0A14_RS30650"
                     /old_locus_tag="F0A14_030645"
     CDS             869..1615
                     /locus_tag="F0A14_RS30650"
                     /old_locus_tag="F0A14_030645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="WP_172689986.1"
                     /translation="MSGGNAVTPTSTSAQARVMLYQPSQRPRLRSGDWTETSFGRCRIE
                     GRLGQRHADVVESILYCAEKRRDVSDGGIELLVDPARVRKTLSDSRYSHERLWSILREC
                     MGAVIEVETPTFRVLGHLIDQVVESPMTRRNPLTGDDRPMWRVRLGMALVMLLERDLSL
                     YYDPAPIARLQYGISQAVARHILSHKIEPLGGWYIDTAIMAVAGKSGSQTMRDGRRRLK
                     EDADELKALGLILDGDRIRKKRADDA"
                     /name="ABC transporter ATPase"
     gene            complement(2115..3150)
                     /locus_tag="F0A14_RS30655"
                     /old_locus_tag="F0A14_030650"
                     /pseudo=""
     CDS             complement(2115..3150)
                     /locus_tag="F0A14_RS30655"
                     /old_locus_tag="F0A14_030650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139446.1"
                     /GO_function="GO:0009009 - site-specific recombinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA];
                     GO:0009294 - DNA-mediated transformation [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /name="integron integrase"
     gene            3309..4172
                     /locus_tag="F0A14_RS30660"
                     /old_locus_tag="F0A14_030655"
     CDS             3309..4172
                     /locus_tag="F0A14_RS30660"
                     /old_locus_tag="F0A14_030655"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     GES-5"
                     /protein_id="WP_012658785.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVEWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MSDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="carbapenem-hydrolyzing class A beta-lactamase GES-5"
     gene            complement(4509..4976)
                     /locus_tag="F0A14_RS30665"
                     /old_locus_tag="F0A14_030660"
     CDS             complement(4509..4976)
                     /locus_tag="F0A14_RS30665"
                     /old_locus_tag="F0A14_030660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172689985.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSFEPSDGLI
                     ENLTDEEKTELQSYIETERQAAEKRTRQYFANSAASRIKEVADTIQGGDFEPSEAWAAD
                     TWAAIEALTKVMRKAGYPKPRKATQKAADAPMADQAGLPLGDAPEAPERGR"
                     /name="hypothetical protein"
     gene            5434..5820
                     /locus_tag="F0A14_RS30670"
                     /old_locus_tag="F0A14_030665"
     CDS             5434..5820
                     /locus_tag="F0A14_RS30670"
                     /old_locus_tag="F0A14_030665"
                     /function="plasmid mobilization"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_014454106.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPEVNREAWAALARTAANLNQIAHRLNAGDALPLAEVRAT
                     LDAFRRDLIGVKHDSEDVEDESEG"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(6011..6145)
                     /locus_tag="F0A14_RS30675"
                     /old_locus_tag="F0A14_030670"
     CDS             complement(6011..6145)
                     /locus_tag="F0A14_RS30675"
                     /old_locus_tag="F0A14_030670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259347331.1"
                     /translation="MILVRVLVAHCHPRRIGLVKAHALHEVSRHGFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            6576..7259
                     /locus_tag="F0A14_RS30680"
                     /old_locus_tag="F0A14_030675"
     CDS             6576..7259
                     /locus_tag="F0A14_RS30680"
                     /old_locus_tag="F0A14_030675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227978814.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSVSLMHEDLKRLPGAVS
                     TETAQTLEPLARLRQDVTQVLEAYDKVTAIQRKTLDELTQQMSASAAQAFEQKAGELDK
                     TISDLSNSLSGLETSISSMKQTAEQVADLPGNLVSAQKDMTQAANKLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            7295..8230
                     /locus_tag="F0A14_RS30685"
                     /old_locus_tag="F0A14_030680"
     CDS             7295..8230
                     /locus_tag="F0A14_RS30685"
                     /old_locus_tag="F0A14_030680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227978815.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLTAREHGRDYLKGRGIT
                     SDTIEHAEKAGMVRYADGGVLFVGYDRAGTAQNATRRAIAPADPVQKRDLRGSDKSYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFFERDDVQAILKRA
                     ERVTVAGENEKNPDAQAKADAGHQKQAQRVAEITGREVRQWTPKPEQGKDLADMNARQV
                     VEIERKRQAEIEAESAAQAPGGYDGPSFGG"
                     /name="hypothetical protein"
     ARG             3310..4169
                     /name="blaGES-5"
                     /gene="blaGES-5"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     GES-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..882
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5805..8224
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.443"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggctatca acattatgga ggcgttccag caggaaccgc cacaactgga cttcatctgg
       61 ccgggctttc tggctggcac cgtgggcgca ctggtggccc ccggcgcgac cggcaagagc
      121 ttctgggcgc tggaagcggc tatgtccatc gcttgcagcg tggcaggcgg tgacgtggtg
      181 aacctcaagc cgcagcacac cgggcgcgtg gtctatctgg ccggtgaaga tcccgaaccc
      241 gccctgatcc ggcgcgttca cgccatcggc cagcacctca accagtcggc ccgcgaagcc
      301 atcgccgaca acctgaccct agagccgatc atgggcaagc gcctgaacgt catggacgag
      361 cggcaccttg cccgcgtggt ggagttctgc gccgatgccc ggctgatcgt gctggacacc
      421 ctgagccgca ttcacagcct cgacgagaac agcaacggcg acatggcgcg gcttgtcgcc
      481 acgctggaac acgtcgcggc cagcaccggg gcgtctgtcc tgtacctgca ccacgtcagc
      541 aagggcagcg cccgcgaagg ccagaccgac cagcagcagg cggcgcgggg cgcttcggcc
      601 ttgatcgaca atgcccgctg gtgcggcttc gttgccaaga tgaccgagga cgaggccaag
      661 cgcctgagcg accgcgccta tgaccggcag cccatcggca acgaccggcg cgggttcttc
      721 gtgcgcttcg gcgtgagcaa gcagaactac gacgctaccc cgcttgacca gtggtacgag
      781 cgccgagacg gcggcgtcct gctgcctgtg gaactgctgg aagtgccgaa cggcaagaaa
      841 gccggatcat tccagaaggg gggcgacgat gagcggtggt aatgcagtta cccctacgag
      901 caccagcgca caagcgcggg tgatgctcta tcagcccagc cagcgcccgc gcctgcggtc
      961 tggcgactgg acagaaacat catttgggcg atgccgtatt gaaggacggc tcgggcaacg
     1021 tcacgccgat gttgtggagt cgattctgta ttgcgccgag aagcgccggg atgtttcgga
     1081 cggtggaatt gaacttctgg ttgacccggc tcgcgtccga aaaacgctgt cagacagccg
     1141 gtattcacac gagcggctat ggtcgatatt gcgcgagtgc atgggcgcag tcatcgaagt
     1201 cgagacgccc actttccgcg tgcttggaca tttgattgat caggtggtcg agtccccaat
     1261 gacgcgccgc aacccactaa cgggggatga tcgcccaatg tggcgggtga ggctcggtat
     1321 ggcgcttgtg atgctgctag agcgcgatct gagcctgtac tacgacccag caccgattgc
     1381 ccggctacag tacggcatca gccaagccgt ggctaggcac atcttgagcc acaaaatcga
     1441 accgctgggc ggctggtata tcgacacggc catcatggcc gtagccggga agtccggcag
     1501 ccaaaccatg cgcgacggcc gtcggcgact gaaagaggac gccgacgaac tgaaggcgtt
     1561 ggggctgatc ctcgacggcg atcggatcag gaaaaaacgg gccgacgacg cctaaagcgt
     1621 ggcacagcgg cccgatagcg tggcacagcg gcccgatagc gtggcacagc ggcccgaaac
     1681 gtggcacagc ggcccggagt aagcaggata ttcaggatat tcagatcctg cgcggcgcag
     1741 cccgcgcttg gtgcgcggtc tggccttgcc atcgcccccc gccttcggcg accccctccg
     1801 ggggtaaagg gccagcgggg agagggggtg gaggccggtt tcccggccta aacccatcag
     1861 gaggcgcttt ctgggggaac cgcagaattc ggaaaaaatc gtacgctaag ctaacggtgt
     1921 tctcgtgaca gctctttgac taggctttct aaggccattc tggggtcgtc tcagaaaacg
     1981 ggaaaaatag cacgctaagg cgcgcgacgg cttttaccta gcagcagtgc aagtctgcac
     2041 ctgcgaggca tgacatgcag gagggcgcta tcgaccctga gccgccatcc agatgaaagc
     2101 accacaaccg tcattcagcc gggcgacaag tgcaaggcca aggcgtccag cgggctggag
     2161 gtgcctccgg cagcgacttt cagcacatgc gtgtagatca tcgtcgtgct cacgtccgaa
     2221 tgccccaaca actcttgcac cgttcggatg tctgtgcctg cttgcagcaa gtgggtggcg
     2281 aatgagtggc gcagggtgtg gacagatacg tgtttggcaa tgccagcctg aactaccgct
     2341 ttttttagtt gccggttcag tctttcctca aacaagtggt ggcggcgctc aacgccggtt
     2401 tgtgggtcca cagacagctt ggccgatgga aacacccaga accaggccca gctctcgccc
     2461 gccctggggt acttgcgctc cagtgcatga ggcagataca cgcctccgcg ccccgtggca
     2521 cggtcctgcc cccacacagc gcggacctga atcagctgcg cccgcaaccg aggtacgagc
     2581 gccctgggca gcatcaccac gcggtccttg tcgcccttgc cgctgcgcac aatgatcgcg
     2641 tggcggtcga aatccacatc cttgacccgc aggcccagcg cttcgcgcag gcgcaaccca
     2701 ctgccgtaaa gcagggcggc caacagcgct tcggtgcccg ccatgtgcga aagcaacgtc
     2761 tgaacctcct gcaccgtcag caccaccgga atccgcttgc gttctggcgg ccgaccaatc
     2821 tgctgcatcc acggcaattc catgcccagc acctgccgat acaagaacaa cagcgcgttg
     2881 agcgcctgcc ggtgggtggc cggcgccact tgcttctcgg tggcgagcat ggtcagaaaa
     2941 ccctcgactt cagcttgccc catttcgcgc ggatgtcgaa acccaccatg gctgcgggcc
     3001 gtccacaaca caaatgcctt ggcccagtag acataagctt tctcggtctg tagaatgcag
     3061 gtagcgaacc cgttcgcgca cctgatcgag cagcttgatg gatcggggag ggaaccagtc
     3121 aggtttgtca tttctgttat acctgttcat gcatccagta tgatgcttta cagaattgat
     3181 ttcaaacact ttttttgggt gccgtgcgac attgtttaac gaccacggtt gtgggtatcc
     3241 ggtgtttggt cagataaacc ataagttaga cgggcgtaca aagataattt ccatctcaag
     3301 ggatcaccat gcgcttcatt cacgcactat tactggcagg gatcgctcac tctgcatatg
     3361 cgtcggaaaa attaaccttc aagaccgatc ttgagaagct agagcgcgaa aaagcagctc
     3421 agatcggtgt tgcgatcgtc gatccccaag gagagatcgt cgcgggccac cgaatggcgc
     3481 agcgttttgc aatgtgctca acgttcaagt ttccgctagc cgcgctggtc tttgaaagaa
     3541 ttgactcagg caccgagcgg ggggatcgaa aactttcata tgggccggac atgatcgtcg
     3601 aatggtctcc tgccacggag cggtttctag catcgggaca catgacggtt ctcgaggcag
     3661 cgcaagctgc ggtgcagctt agcgacaatg gggctactaa cctcttactg agagaaattg
     3721 gcggacctgc tgcaatgacg cagtattttc gtaaaattgg cgactctgtg agtcggctag
     3781 accggaaaga gccggagatg agcgacaaca cacctggcga cctcagagat acaactacgc
     3841 ctattgctat ggcacgtact gtggctaaag tcctctatgg cggcgcactg acgtccacct
     3901 cgacccacac cattgagagg tggctgatcg gaaaccaaac gggagacgcg acactacgag
     3961 cgggttttcc taaagattgg gttgttggag agaaaactgg tacctgcgcc aacgggggcc
     4021 ggaacgacat tggttttttt aaagcccagg agagagatta cgctgtagcg gtgtatacaa
     4081 cggccccgaa actatcggcc gtagaacgtg acgaattagt tgcctctgtc ggtcaagtta
     4141 ttacgcaact catcctgagc acggacaaat agttgacgcc cgtctaacaa ttcgttcaag
     4201 ccgacgttgc ttcgtggcgg cgcttgcgtg ctacgctaag cttcgcacgc cgcttgccac
     4261 tgcgcaccgc ggcttaactc aggcgttgag cgactgctta cccgtttgac ggtcggccgc
     4321 tcctggccgg gagccgaaac ccagagttgg ggcgtcaacc tgatctatac ctcaactggg
     4381 cgtcagtcgg ctcactggca tcgcgtcaga ctagggccgc tttctgcatt atctgacgcc
     4441 taaacgtcaa actctatggc ctcaacctga caggccgcat gcttagcgta cgattttgtc
     4501 cgttttctct accgtcccct ttctggcgct tctggcgcat cccccaaggg caaccccgcc
     4561 tgatccgcca tcggcgcgtc agcggccttc tgtgtcgctt tgcggggctt cggatagccc
     4621 gctttgcgca tgactttggt cagcgcctcg attgccgccc aggtatccgc cgcccacgcc
     4681 tcggacggct cgaaatcgcc cccttggatc gtatcggcaa cctctttaat ccgactggcg
     4741 gctgagttag cgaaatactg tcgggttcgt ttttctgctg cctgtcgctc cgtttcaata
     4801 tatgactgta gttctgtttt ttcttcgtct gtcagatttt caattaagcc gtctgacggc
     4861 tcaaatgaat atacgtcaat agaccccatc atcttaacga tggcgcggcg tttttccttg
     4921 tcataccccc ggtaggcgag cacctgaacc cggcgacctt ggcgcttaaa ctgcatggcg
     4981 gtttctccgt ttgactgtgt aattgtattc tagtctgaca gtcgtacgct gtcaagtgtt
     5041 tgcagtcggt ttctttttta atacgactgg cattcttaaa tgatccttca ctgtcgtatt
     5101 aaatagcgat tgacactctt tcccgattcc gatactatag tggtatggta tcacttcgcc
     5161 ccgtcgcttt gcccaccccc tctccccgct ggcccattac cgcccttggg cggtcgggac
     5221 gagagcgcag cgagcgggcc gggtgggtgg ctgtttggtg cttgcatgga gatatgctat
     5281 tatggtattt gcgcggtggt ttggcaggga gcaaggtgta accagcgctt cgcttggttc
     5341 tcaccctccg gccttcggcc tacgcccccg ctcccttcgg tcggggggtt gctgcgcgtt
     5401 tcgcttcgct tcacttgctg gagggcttcg cccatgaaca ctgacgaatc caagcgccgc
     5461 cgccgtgggc ctgcgccgct cgacgccacg gacaagcgcg ggcataccgt ttcggtgcgc
     5521 ctcaacgatg ccgagcttgc gcggctggat tcgcagcgtg acgcggtgca gatgcagcgc
     5581 ggtgagtacc tacgcgccgc cgcccttcat cgcctgccgc ccactatccc ggaagtgaac
     5641 cgggaagcgt gggccgcgtt ggcccgcacg gcggccaacc tgaaccagat cgcgcaccgg
     5701 ctgaacgctg gtgacgcgct cccgctggcc gaggtgcggg caacgctcga cgccttcagg
     5761 cgtgacctga taggcgtcaa gcacgactcg gaggacgtgg aagatgaaag cgaaggttag
     5821 ccgtggtggc ggttttcgcg gtgcgctgaa ctacgttttt gacgttggca aggaagccac
     5881 gcacacgaaa aacgcggaac gtgtcggcgg caacatggcc gggaatgacc cccgcgaact
     5941 gtcgcgggag ttctcagccg tgcgccagtt gcgcccggac atcggcaagc ccgtctggca
     6001 ttgctcgctg tcactgcctc ccggtgagcg cctgagcgcc gagaagtggg aagccgtgac
     6061 gactgacttc atgcagcgca tgggctttga ccagaccaat acgccgtggg tggcagtgcg
     6121 ccaccaggac acggacaagg atcacattca catcgtggcc agccgggtag ggctggacgg
     6181 gaaagtgtgg ctgggccagt gggaagcccg ccgcgccatc gaggcgaccc aagagcttga
     6241 gcatacccac ggactgaccc tgacgccggg gctgggcgat gcacgggccg agcgccggaa
     6301 gctgaccgac aaggagatca atatggccgt gagaacgggc gacgaaccgc cgcgccagcg
     6361 tctgcaacgg ctgctggatg aggcggtgaa ggacaagccg accgcgccag aactggccga
     6421 gcgactacag gccgcaggcg taggcgtccg ggcaaacctc gccagcacag ggcgcatgaa
     6481 cggcttttcc tttgaagtgg ccggggtggc gttcaagggc agcgacttgg gcaagggcta
     6541 cacatgggcg gggctacaga aagcaggggt gacttatgac gaagctagag accgtgcggg
     6601 ccttgaacga ttccggccca cagttgcaga tcgtggagag cgtcaggacg ttgcagcagt
     6661 ccgtgagcct gatgcacgag gacttgaagc gcctaccggg cgcagtctcg accgagacgg
     6721 cgcagacctt ggagccgctg gcccgactcc ggcaggacgt gacgcaggta ctggaagcct
     6781 acgacaaggt gacggccatc cagcgcaaga cgctggacga gctgacgcag cagatgagcg
     6841 cgagcgcggc gcaggcgttc gagcagaagg ccggggagct ggacaagacc atctccgacc
     6901 tgtccaacag cctgtcaggg ctggaaacga gcatcagcag catgaagcag accgcggagc
     6961 aggtggcgga cttgccgggc aatctggtga gcgcacagaa ggacatgacg caagccgcca
     7021 acaaactgac cgaggcagcg aacgagacgc gcccgcgcct ttggcggcag gcgctggggc
     7081 tgattctggc cggggccgtg ggcgcgatgc tggtagcgac tgggcaagtc gctttaaaca
     7141 ggctagtgcc gccaagcgac gtgcagcaga cggcggattg ggccaacgcg atttggaaca
     7201 aggccacgcc caaggagcgc gagttgctgg aacagatcgt caatcggccc gcgaactaga
     7261 tccgaccgcc taccttgagg cgagcggcta caccgtgaag cgcgaagggc gacacctgtc
     7321 cgtcaaggct ggcggtgatg aggcgtaccg cgtgacccgg cagcaggacg ggcgctggct
     7381 ctggtgcgac cgctacggca acgacggcgg ggacaatatc gaccttgtgc gcgagatcga
     7441 acctggcacc ggctacgccg aggccgtcta tcggctttca ggtgcgccga cagtccggca
     7501 gcaaccgcgc ccgagcgagc cgaagcgcca accgccgcag ctaccggcgc aagggctgac
     7561 agcccgcgag catggccgcg actacctcaa gggccgtggc atcacctcgg acaccatcga
     7621 gcacgccgaa aaggcgggca tggtgcgcta tgcagacggc ggggtgctgt tcgtcggcta
     7681 cgaccgtgca ggcaccgcgc agaacgccac acgccgcgcc attgcccccg ctgacccggt
     7741 gcaaaagcgc gacctacgcg gcagcgacaa gagctatccg ccgatcctgc cgggcgaccc
     7801 ggcaaaggtc tggatcgtgg aaggtggccc ggatgcgctg gccctgcacg acatcgcaaa
     7861 gcgcagcggc cagcagccgc ccaccgtcat cgtgtcgggc ggggcgaacg tgcgcagctt
     7921 ctttgagcgt gacgacgtgc aagcgatcct gaagcgggcc gagcgcgtca ccgtggccgg
     7981 ggagaacgag aagaacccag acgctcaggc caaggccgac gccgggcacc agaagcaggc
     8041 gcagcgggtg gccgaaatca ccgggcgcga ggtgcgccaa tggacgccga agcccgagca
     8101 aggcaaggac ttggccgaca tgaacgcccg ccaggtggtc gagatcgagc gcaagcgaca
     8161 ggccgagatc gaggccgaga gcgcagcgca agcgccgggg gggtatgatg gccccagctt
     8221 cggcggataa gagcgcctgt cagtcggtgt gatagaccga ccgacaggct gaccatcatc
     8281 aacctgtaca ggaggccgat
//
