LOCUS       NZ_CP113513             7689 bp    DNA     circular CON 19-NOV-2023
DEFINITION  Bacteroides ovatus strain CP926 plasmid unnamed, complete sequence.
ACCESSION   NZ_CP113513
VERSION     NZ_CP113513.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31888783
            Assembly: GCF_030168555.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides ovatus
  ORGANISM  Bacteroides ovatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Roenne,M.E., Tandrup,T., Madsen,M., Hunt,C.J., Myers,P.N.,
            Moll,J.M., Holck,J., Brix,S., Strube,M.L., Aachmann,F.L., Wilkens,C.
            and Svensson,B.
  TITLE     Three Alginate Lyases Providing a New Gut Bacteroides ovatus Isolate
            with the Ability to Grow on Alginate
  JOURNAL   Appl Environ Microbiol (2023) In press
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Roenne,M.E., George,J., Bahl,M.I., Strube,M.L., Svensson,B. and
            Licht,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2022) DTU Bioengineering, Technical University of
            Denmark, Soeltofts Plads 224, Kongens Lyngby 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-DEC-2021
            Assembly Method                   :: Flye v. 2.9.b1768
            Assembly Name                     :: Bacteroides ovatus CP926
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/19/2023 08:39:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,176
            CDSs (with protein)               :: 5,176
            Genes (RNA)                       :: 95
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 89 of 153
            Pseudo Genes (incomplete)         :: 58 of 153
            Pseudo Genes (internal stop)      :: 25 of 153
            Pseudo Genes (multiple problems)  :: 18 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113513.1.
            Bacteria and source DNA available from Research group Enzyme and
            Protein Chemistry, Department of Biotechnology and Biomedicine,
            DTU, Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Bacteroides ovatus"
                     /mol_type="genomic DNA"
                     /strain="CP926"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:28116"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.78 N 12.52 E"
                     /collection_date="2000"
     gene            complement(join(<7479..7689,1..358))
                     /locus_tag="OU990_RS00005"
                     /old_locus_tag="OU990_00005"
                     /pseudo=""
     CDS             complement(join(<7479..7689,1..358))
                     /locus_tag="OU990_RS00005"
                     /old_locus_tag="OU990_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            660..911
                     /locus_tag="OU990_RS00010"
                     /old_locus_tag="OU990_00010"
     CDS             660..911
                     /locus_tag="OU990_RS00010"
                     /old_locus_tag="OU990_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295507.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            908..1183
                     /locus_tag="OU990_RS00015"
                     /old_locus_tag="OU990_00015"
     CDS             908..1183
                     /locus_tag="OU990_RS00015"
                     /old_locus_tag="OU990_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            1194..1391
                     /locus_tag="OU990_RS00020"
                     /old_locus_tag="OU990_00020"
     CDS             1194..1391
                     /locus_tag="OU990_RS00020"
                     /old_locus_tag="OU990_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295503.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            1584..1883
                     /gene="mbpA"
                     /locus_tag="OU990_RS00025"
     CDS             1584..1883
                     /gene="mbpA"
                     /locus_tag="OU990_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_004327437.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="mbpA"
     gene            1880..2674
                     /gene="mbpB"
                     /locus_tag="OU990_RS00030"
                     /old_locus_tag="OU990_00025"
     CDS             1880..2674
                     /gene="mbpB"
                     /locus_tag="OU990_RS00030"
                     /old_locus_tag="OU990_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_007560590.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="mbpB"
     gene            2631..3083
                     /gene="mbpC"
                     /locus_tag="OU990_RS00035"
                     /old_locus_tag="OU990_00030"
     CDS             2631..3083
                     /gene="mbpC"
                     /locus_tag="OU990_RS00035"
                     /old_locus_tag="OU990_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            3103..3333
                     /locus_tag="OU990_RS00040"
                     /old_locus_tag="OU990_00035"
     CDS             3103..3333
                     /locus_tag="OU990_RS00040"
                     /old_locus_tag="OU990_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
     gene            complement(3370..4395)
                     /locus_tag="OU990_RS00045"
                     /old_locus_tag="OU990_00040"
     CDS             complement(3370..4395)
                     /locus_tag="OU990_RS00045"
                     /old_locus_tag="OU990_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004295509.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            4808..5059
                     /locus_tag="OU990_RS00050"
                     /old_locus_tag="OU990_00045"
     CDS             4808..5059
                     /locus_tag="OU990_RS00050"
                     /old_locus_tag="OU990_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295507.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            5056..5331
                     /locus_tag="OU990_RS00055"
                     /old_locus_tag="OU990_00050"
     CDS             5056..5331
                     /locus_tag="OU990_RS00055"
                     /old_locus_tag="OU990_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            5342..5539
                     /locus_tag="OU990_RS00060"
                     /old_locus_tag="OU990_00055"
     CDS             5342..5539
                     /locus_tag="OU990_RS00060"
                     /old_locus_tag="OU990_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295503.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            5732..6031
                     /gene="mbpA"
                     /locus_tag="OU990_RS00065"
     CDS             5732..6031
                     /gene="mbpA"
                     /locus_tag="OU990_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_004327437.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="mbpA"
     gene            6028..6822
                     /gene="mbpB"
                     /locus_tag="OU990_RS00070"
                     /old_locus_tag="OU990_00060"
     CDS             6028..6822
                     /gene="mbpB"
                     /locus_tag="OU990_RS00070"
                     /old_locus_tag="OU990_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_007560590.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="mbpB"
     gene            6779..7231
                     /gene="mbpC"
                     /locus_tag="OU990_RS00075"
                     /old_locus_tag="OU990_00065"
     CDS             6779..7231
                     /gene="mbpC"
                     /locus_tag="OU990_RS00075"
                     /old_locus_tag="OU990_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            7251..7481
                     /locus_tag="OU990_RS00080"
                     /old_locus_tag="OU990_00070"
     CDS             7251..7481
                     /locus_tag="OU990_RS00080"
                     /old_locus_tag="OU990_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
ORIGIN
        1 ttttatattt tgggttagat ataaatccgc acttccacca ttcctcgtca tcttcgtaag
       61 taaaaaaacg tcctgccaaa gaatcgagtg cggctatgat ttcattgtaa tcacgtccgg
      121 aaaaaataac gtcagataca tgcatttgtg catcaacatc ccaaagtcca tgctcaaact
      181 tgcgtttcgt gcctgcataa tctttcagtt ttactccctt tagttcagct tggcacgctt
      241 ccagtatgcg aagaactatt ctttgttcgt gtatggacat gtcttgctgt tttgaccatg
      301 tatataccca tgaaacaaca acatctttat tcttggtaat aggtagtttc tttttcataa
      361 ctaaactccc aaagtgtggg gaaacacatg ttctaatccc tttatttatg ggcgtttgcg
      421 aagaagtgat agagcatatt acagacgatg cttataacag acaaggggaa tttatagagc
      481 taagtggttg atttactgtc ggaaaaagaa aaacgatagg gaaaaagagc aaagcaaaac
      541 caaccaacgg gcggtaggat tggaaacggg tttttatggc gtgtacacgt ttgtgtacaa
      601 aatgtttgta ttttattgaa atatagctat ctttgtgctg aatttaaaag caaacagata
      661 tggaagcatt atcagtaaga gaataccgta acaacctcgc agcgtctttc accaaagctg
      721 acaatggcga gcaggtacta attcgcagga aaaacgagat ttacgctttg gtaaaagttg
      781 gtcgtgaaga tttgatgata accccggagc tgcaagcaag gattgacaag gcaagggaag
      841 aaatcaaatc cggaaagtgt gttaccctca aaagcagtga ggatattgac gcttatttcg
      901 atagtctatg atgtacacaa ttagggtttc cgacggggtg gataaggtga ttgccaaatg
      961 gaagaaatca aaccccaatt tgttcaagaa gtataagaaa atctacaaag aattgttaga
     1021 gcaccctaag actgggcttg ggcatcccga agcgttaagg ggcggtgggg atattacatg
     1081 gtctcgacac attaccgccc atgaccggat aatctatgac atttacgagg aagtggtaga
     1141 ggtctacatc ttggaggtag aggggcatta caacgataaa tgataaaact gttatggtcg
     1201 aatattgtgt ttactggtta gagaacggcg agcctatgca cgaggtgttc tcttctcttg
     1261 ccgccgccga gatgtactca tgtgcgataa gagggaaaga aaacattgaa tgggtggagg
     1321 tgtccgaaga agaaaccatt gatttggacg aactggaaga catgttcccg gatgacttct
     1381 gcggcgtgta atccccttgg gtagaaatgg ggtagaattt gttaggctgt gggggatttt
     1441 ctaacacgcc ggggcttttt gctgggcggt tggcgtggat gtattcccat gcggcatgtg
     1501 tatatatagc aagaagtgtc cttgtcggac aattcttgct tttctcgctt tgctcaaaaa
     1561 gattttaaga ttacctttgt ggcatggaac taagacggaa cgaaaagatt acattccggt
     1621 gtaccgaact tgaaaaggac gcacttgcgg agcaggcagc ccggtgcagt ctaagcgtat
     1681 cggaatactg ccggagtttg tcccttgggg ggcgcccaag ggagaggtac accgaggagg
     1741 aacggcagct tctccgggac atagcccagt tgaaaggaac gctccaacgg ctgaacaact
     1801 acttcggtgg tcgccagtac cgggaagtgt tcgaggagaa ccgggcacta attacagagc
     1861 tcaaaaaaat actctcacga tgatagggaa agggaaaagc atatcacatg gtgtggcggc
     1921 attggagtac gacctcgcca aagagataaa cgggcaggca gtagccaccg agatagcccg
     1981 gcatgaactt tatgggtgta ctggtgcgga aatggtgcag gaaatgaaac cgtatcacat
     2041 tgattttccg aacgtaaaaa acaactgcct acgtttcgag gtcagcccct cgatagagga
     2101 aagcgccacc tttacggatg cggactgggc ggaacttggt aacgacttca tgcagcgcat
     2161 ggggttggcg aaccaccagt acatcatcat ccggcacagc ggtacggaga gcaagaaaga
     2221 gcaagcccac ctgcacatac tggcaaaccg ggtttccctt tccggggaac tgtaccggga
     2281 caactggata gggaaaaagg caacggaagc cgccaatgcc atagccaagg aacggaactt
     2341 tgtacagtcg caggacattg gcaaagtcaa caaagcggaa atcaaggaag ccatggacgg
     2401 cgtattgaag aagatgcagg gctttgactt taccaaattc aaggaagaac ttggtaagag
     2461 aggttttaaa gtccgggaag ccagggcaag caccgggaaa cttaacggct actatgtcac
     2521 agcccgaagc ggtacggagt ataaggcaag cgagataggg aaaggctaca ctctcgccca
     2581 tatcgagcgg acacaaagca aactgaagtg caactcaatg aacatatctc atggaaacaa
     2641 actcacaccc ggaagcggca gcttccaacg ttaagcaggg acagtacagc ccccggaaaa
     2701 gaaaagatac aacgccaagg gtaaacccgc agcttgcggt agaactggtg tatcaagaac
     2761 taaagcgtgt tgaagtctat acgaagcgca tagaggacgc aacagcccga aaggtacaaa
     2821 tagacgggaa aagccttgaa agtgccgaaa atcgcctaaa aaacgtgttg gcggactttg
     2881 aacggcaggg gtacagaatg aaaaacggcg gttatgtgga caaacgaatt tcgttctact
     2941 caatcctttg tgctgttatc tccctattgt tcgcttgttt catgtgctat ctttggacgg
     3001 atgcagccaa agaccgggac aactacaagc agtattatga atactaccaa gagcaggcaa
     3061 gggaacaaaa ggggaataaa taaacatcta actttacgaa ttatgggaac aacggaacac
     3121 gaagaacccc ggttcttctt catactgaac aagggtgcga agtccggcgg cgaaatcacc
     3181 catgccgtcc taaacggtag tatcgtttcg aagccggcag gctgggacgc ctttcatggg
     3241 cttgcactgg cgagagagaa actaagcagc gaggaaatac aacagcagat gaaagaactt
     3301 ggtgtagaaa tggaaatcgt tcctctgatt tgacaagatt acccggagtg tttcagccgg
     3361 gtaatcttgt tacttatcct tgattttccc tttaagggcg tttataatcc accctttcgg
     3421 attgttcttt tctcgtgatt ttccgtttag gagagccagt tctccgataa ggtcggttat
     3481 cttttcttgt gccgttatga atgtctcttt gttccggttt atctcttccg atgtgaagcc
     3541 gcaggaataa cggaggtact cgtacacatg gctgtctatc tgatatcgtg ctgtaacctt
     3601 tgcttgcaat tctttccctt ccagttcttc atctctgaac tgtggttgat agaccgggta
     3661 gaacctaaac ccggtcacct tgctacgttt gttgtttggg ttttcacgca ctttcacgta
     3721 gttgaaagtg tatgggcagc tctcatcaag cgcagccttt gctggtttca aaactctttc
     3781 ctcaaaatga tctattctgt cttttccgtt cttgtccttg tatttgtccg caggtatgcc
     3841 aagacggtct ttcaagtttt caagggatat atccaaaggg tacacctgcc cactcattaa
     3901 catgtaaaac ctaagggaat acccagtagg tagcgcaaga gccttgttta actcaaattc
     3961 tctgtacccc ttggcgaact tggtaaacac atcccaaagg tcgttagaca cacgaaaggt
     4021 tataatgcca gtgcgttttt tatattttgg gttagatata aatccgcact tccaccattc
     4081 ctcgtcatct tcgtaagtaa aaaaacgtcc tgccaaagaa tcgagtgcgg ctatgatttc
     4141 attgtaatca cgtccggaaa aaataacgtc agatacatgc atttgtgcat caacatccca
     4201 aagtccatgc tcaaacttgc gtttcgtgcc tgcataatct ttcagtttta ctccctttag
     4261 ttcagcttgg cacgcttcca gtatgcgaag aactattctt tgttcgtgta tggacatgtc
     4321 ttgctgtttt gaccatgtat atacccatga aacaacaaca tctttattct tggtaatagg
     4381 tagtttcttt ttcataaacg attgatttgc tgcaaatata cacaatcccc acattagaac
     4441 aaaaaaagag gggtaaaact cccaaagtgt ggggactaat ttacttttaa actcccaaag
     4501 tgtggggact aaactcccaa agtgtgggga aacacatgtt ctaatccctt tatttatggg
     4561 cgtttgcgaa gaagtgatag agcatattac agacgatgct tataacagac aaggggaatt
     4621 tatagagcta agtggttgat ttactgtcgg aaaaagaaaa acgataggga aaaagagcaa
     4681 agcaaaacca accaacgggc ggtaggattg gaaacgggtt tttatggcgt gtacacgttt
     4741 gtgtacaaaa tgtttgtatt ttattgaaat atagctatct ttgtgctgaa tttaaaagca
     4801 aacagatatg gaagcattat cagtaagaga ataccgtaac aacctcgcag cgtctttcac
     4861 caaagctgac aatggcgagc aggtactaat tcgcaggaaa aacgagattt acgctttggt
     4921 aaaagttggt cgtgaagatt tgatgataac cccggagctg caagcaagga ttgacaaggc
     4981 aagggaagaa atcaaatccg gaaagtgtgt taccctcaaa agcagtgagg atattgacgc
     5041 ttatttcgat agtctatgat gtacacaatt agggtttccg acggggtgga taaggtgatt
     5101 gccaaatgga agaaatcaaa ccccaatttg ttcaagaagt ataagaaaat ctacaaagaa
     5161 ttgttagagc accctaagac tgggcttggg catcccgaag cgttaagggg cggtggggat
     5221 attacatggt ctcgacacat taccgcccat gaccggataa tctatgacat ttacgaggaa
     5281 gtggtagagg tctacatctt ggaggtagag gggcattaca acgataaatg ataaaactgt
     5341 tatggtcgaa tattgtgttt actggttaga gaacggcgag cctatgcacg aggtgttctc
     5401 ttctcttgcc gccgccgaga tgtactcatg tgcgataaga gggaaagaaa acattgaatg
     5461 ggtggaggtg tccgaagaag aaaccattga tttggacgaa ctggaagaca tgttcccgga
     5521 tgacttctgc ggcgtgtaat ccccttgggt agaaatgggg tagaatttgt taggctgtgg
     5581 gggattttct aacacgccgg ggctttttgc tgggcggttg gcgtggatgt attcccatgc
     5641 ggcatgtgta tatatagcaa gaagtgtcct tgtcggacaa ttcttgcttt tctcgctttg
     5701 ctcaaaaaga ttttaagatt acctttgtgg catggaacta agacggaacg aaaagattac
     5761 attccggtgt accgaacttg aaaaggacgc acttgcggag caggcagccc ggtgcagtct
     5821 aagcgtatcg gaatactgcc ggagtttgtc ccttgggggg cgcccaaggg agaggtacac
     5881 cgaggaggaa cggcagcttc tccgggacat agcccagttg aaaggaacgc tccaacggct
     5941 gaacaactac ttcggtggtc gccagtaccg ggaagtgttc gaggagaacc gggcactaat
     6001 tacagagctc aaaaaaatac tctcacgatg atagggaaag ggaaaagcat atcacatggt
     6061 gtggcggcat tggagtacga cctcgccaaa gagataaacg ggcaggcagt agccaccgag
     6121 atagcccggc atgaacttta tgggtgtact ggtgcggaaa tggtgcagga aatgaaaccg
     6181 tatcacattg attttccgaa cgtaaaaaac aactgcctac gtttcgaggt cagcccctcg
     6241 atagaggaaa gcgccacctt tacggatgcg gactgggcgg aacttggtaa cgacttcatg
     6301 cagcgcatgg ggttggcgaa ccaccagtac atcatcatcc ggcacagcgg tacggagagc
     6361 aagaaagagc aagcccacct gcacatactg gcaaaccggg tttccctttc cggggaactg
     6421 taccgggaca actggatagg gaaaaaggca acggaagccg ccaatgccat agccaaggaa
     6481 cggaactttg tacagtcgca ggacattggc aaagtcaaca aagcggaaat caaggaagcc
     6541 atggacggcg tattgaagaa gatgcagggc tttgacttta ccaaattcaa ggaagaactt
     6601 ggtaagagag gttttaaagt ccgggaagcc agggcaagca ccgggaaact taacggctac
     6661 tatgtcacag cccgaagcgg tacggagtat aaggcaagcg agatagggaa aggctacact
     6721 ctcgcccata tcgagcggac acaaagcaaa ctgaagtgca actcaatgaa catatctcat
     6781 ggaaacaaac tcacacccgg aagcggcagc ttccaacgtt aagcagggac agtacagccc
     6841 ccggaaaaga aaagatacaa cgccaagggt aaacccgcag cttgcggtag aactggtgta
     6901 tcaagaacta aagcgtgttg aagtctatac gaagcgcata gaggacgcaa cagcccgaaa
     6961 ggtacaaata gacgggaaaa gccttgaaag tgccgaaaat cgcctaaaaa acgtgttggc
     7021 ggactttgaa cggcaggggt acagaatgaa aaacggcggt tatgtggaca aacgaatttc
     7081 gttctactca atcctttgtg ctgttatctc cctattgttc gcttgtttca tgtgctatct
     7141 ttggacggat gcagccaaag accgggacaa ctacaagcag tattatgaat actaccaaga
     7201 gcaggcaagg gaacaaaagg ggaataaata aacatctaac tttacgaatt atgggaacaa
     7261 cggaacacga agaaccccgg ttcttcttca tactgaacaa gggtgcgaag tccggcggcg
     7321 aaatcaccca tgccgtccta aacggtagta tcgtttcgaa gccggcaggc tgggacgcct
     7381 ttcatgggct tgcactggcg agagagaaac taagcagcga ggaaatacaa cagcagatga
     7441 aagaacttgg tgtagaaatg gaaatcgttc ctctgatttg acttgcaatt ctttcccttc
     7501 agttcttcat ctctgaactg tggttgatag accgggtaga acctaaaccg gtcaccttgc
     7561 tgcgtttgtt gtttgggttt tcacgcactt tcacgtagaa gcaggtgtat gggcaactct
     7621 catcaagcgc agcctttgct ggtttcaaaa ctctttcctc aaaatgatct attctgtctt
     7681 ttccgttct
//
