LOCUS       NZ_CP113492             2576 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Escherichia coli strain GTEN_23 plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP113492
VERSION     NZ_CP113492.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11875193
            Assembly: GCF_026689355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Yorki,S., Shea,T., Worby,C.J., Cuomo,C., LaRocque,R.C., Manson,A.L.
            and Earl,A.M.
  TITLE     Comparison of long and short read metagenomic assembly for
            low-abundance species and resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Yorki,S., LaRocque,R.C., Worby,C.J., Shea,T., Earl,A.M.,
            Walker,B.J., Manson,A.L., Sridhar,S., Turbett,S., Becker,M.,
            Oliver,E., Bronson,R., Leung,D., Knouse,M., Hagmann,S., Kelly,P.,
            Harris,J. and Ryan,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2022) Infectious Diseases & Microbiome, Broad
            Institute of MIT and Harvard, 75 Ames St., Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: GTEN_23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 488.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 06:31:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,428
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,082
            CDSs (with protein)               :: 5,082
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 72 of 222
            Pseudo Genes (incomplete)         :: 153 of 222
            Pseudo Genes (internal stop)      :: 47 of 222
            Pseudo Genes (multiple problems)  :: 48 of 222
            Pseudo Genes (short protein)      :: 3 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113492.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GTEN_23"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: Massachusetts"
                     /collected_by="Massachusetts General Hospital"
     gene            complement(join(2349..2576,1..87))
                     /locus_tag="FG550_RS27020"
     CDS             complement(join(2349..2576,1..87))
                     /locus_tag="FG550_RS27020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     enzyme"
                     /protein_id="WP_268101418.1"
                     /translation="MKVEDFDWQTSEELISSWKKESGYRNDFCKKCGSTVPNILRGQPY
                     VWLPLGLLNENIKADCCGDFCTDDSMMWDCVRSANSNKKAVSSLFDLLVMLNVEQAKLE
                     "
                     /name="glutathione-dependent formaldehyde-activating
                     enzyme"
     gene            complement(1360..1551)
                     /locus_tag="FG550_RS27025"
     CDS             complement(1360..1551)
                     /locus_tag="FG550_RS27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_047402702.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNDQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1970..2083
                     /locus_tag="FG550_RS27030"
     CDS             1970..2083
                     /locus_tag="FG550_RS27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059446996.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          1427..2050
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.603"
                     /coverage="99.0"
                     /evalue="1.53e-168"
ORIGIN
        1 gcagaaatcg ttacggtaac cactttcctt tttccatgaa gaaatcaatt cctctgaggt
       61 ttgccagtca aaatcctcaa ctttcactat ggcagctaag ttatgaccta ctccagactg
      121 tcttcggcat agggaacagt ggcatctata aaattttcag gctgattttt tatcgtgaat
      181 ttaacagcac cacacaaaca tgacccacta atcaaaataa tctccatcaa aataaaatac
      241 gtatttacca taacatctct tgcccgcacc ccctgagagg ccctcagcgc gtttctgccg
      301 cgtcctctgc gtttccggca cctttccctg tccatatcgt gaaaaaacgc gcctgatgat
      361 ttctgcgcgc ctgcatggct atgcacggaa gtgaatggcg gccggtttcg gacgaatgct
      421 ggcactaaag gcgaattttg aacaaaaaaa acggacatcg agggtcagga tgtccgacaa
      481 aatttgcaca aacagtcagc cggtaccgat cagagagccg gcaaataagt atgacagtaa
      541 aaagtggtta tcaactgccc ccgcgctgcg gattgcgcag ttttaaggct ttggccagtg
      601 caggcaggcc cggccggaat caggggatct ggtaacggtg aacgggtgca cagttgactg
      661 tgcggccgtg aggcgttggc caagccgcca ggcgcggcag cttccggtgg gtacaaacgc
      721 cagcactttg cgaatcctgc cctcgatctg aacaaaagca aaatttcgtg cgtacgaaat
      781 tcagggtatc aggctgcgcc tgatgaaatg tttttggtgc cggcggtact ccaaaaacac
      841 atcttgtccg acagttttgt ggtgccggcg aagcggcggt ggccacaaaa catttcctgt
      901 ccccatcgat tcattcgaga aacataagag gcgaagaagg ggtcagtttg gagatcggaa
      961 ttttttgtac gttaagcgca ttatttaacc aatcttggtt cgtaaggggt tgattttgcg
     1021 tcagtggtta tcgcttttaa gttgtcgctc ttgcagtatc gcatttaggt tatccgatta
     1081 aggtcaaacc tctgaaaatg ccgtatagcg cgggagtaca ccctattgct ataggtaagt
     1141 ctgttcaaaa aacaggctta ccgtacaata attctctata tccaaactga ccccatacac
     1201 ctaacgcagc tgcgattttt atcgcaactt catgaaaaat aaataaaaaa gaaaaaataa
     1261 agggattctg ggtattacta gggtaataat gttaagcacc aagacaatga aaattaaggc
     1321 gatgccggta ttactccggt aatacccaga acggagccat tacgcaccgt cctgctgatc
     1381 attcaggcgc gctgacaggc ttcggtaaag gcgttccgca tcctcatgga gtttctcgca
     1441 gaaatcagcc tcatgatcca gatccagctc attaagcctg tccagaagaa gcagggactg
     1501 atcctggatg aatttagcca tgttaacggc tgtcttttgt tgtttgttca tgaaggcccc
     1561 ggcgcgaaag tgcttactgt acgttctgat ggttttccgc ttcctcgctc attcgctcac
     1621 tccgttcggt catgagtctg cggcgagcgg agggaggata agcagataac gtaacggtaa
     1681 gcataacgaa agctaaggac aagggcgaaa aggccacgcc ccggcggggc gtttttccat
     1741 aggctccgcc cccctgacaa gcatcacaga atcggacgct caaatcagtg gtggcgaaac
     1801 ccgacaggac tataaagata ccaggcgttt ccccctggtg gctccctcgt gcgctctcct
     1861 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     1921 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     1981 ccccgttcag tccgaccgct gcgccttgtc cggtaactat catcttgagt ccaacccgga
     2041 aagacacgac aaagcgccac tggcagcagc cactggtaac tgatttagag gagatggact
     2101 tgaagtcatg tgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     2161 caagccagtt acctcggttc aaagagttgc tagctctgag taccttcgaa aaaccgccct
     2221 gcaaggcggt tttttcgttt gcgacaacta atctacgcgg taacccctta tcaggtcaag
     2281 ataactatcc atcagtgcat ctttccaata caatccatta agcaacatcc ctaaggcgtg
     2341 ctcgctatct attcaagttt agcctgctca acattgagca taaccaataa gtcgaacagg
     2401 ctacttaccg cttttttgtt actattcgct gagcgcacac agtcccacat catcgaatcg
     2461 tctgtacaaa aatcaccaca gcagtcagct ttaatattct cgttaagtag cccaagtggt
     2521 agccacacat aaggctgccc cctcaaaata tttggaacag tcgaaccaca tttttt
//
