LOCUS       NZ_CP113232            20673 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Staphylococcus aureus strain BIAI 158 plasmid pBIAI158, complete
            sequence.
ACCESSION   NZ_CP113232
VERSION     NZ_CP113232.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31815319
            Assembly: GCF_026636215.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20673)
  AUTHORS   Walker,J., Duran Ramirez,J. and Hanson,B.
  TITLE     Staphylococcus aureus Breast Implant Infection Isolates Display
            Recalcitrance to Antibiotic Pocket Irrigants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20673)
  AUTHORS   Walker,J., Duran Ramirez,J. and Hanson,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) Center for Antimicrobial Resistance and
            Microbial Genomics, The University of Texas Health Sciences Center,
            1200 Pressler St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2022
            Assembly Method                   :: flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 03:27:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,698
            Genes (coding)                    :: 2,610
            CDSs (with protein)               :: 2,610
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 56 of 88
            Pseudo Genes (incomplete)         :: 28 of 88
            Pseudo Genes (internal stop)      :: 14 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113232.1.
            Bacteria and source DNA available from Walker Laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BIAI 158"
                     /isolation_source="Breast"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pBIAI158"
                     /geo_loc_name="USA: St. Louis"
                     /lat_lon="38.37 N 90.11 W"
                     /collection_date="2019"
                     /collected_by="Margaret Olsen"
     gene            1..339
                     /locus_tag="OSZ57_RS13770"
                     /old_locus_tag="OSZ57_13770"
     CDS             1..339
                     /locus_tag="OSZ57_RS13770"
                     /old_locus_tag="OSZ57_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            344..529
                     /locus_tag="OSZ57_RS13775"
                     /old_locus_tag="OSZ57_13775"
     CDS             344..529
                     /locus_tag="OSZ57_RS13775"
                     /old_locus_tag="OSZ57_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(756..1499)
                     /locus_tag="OSZ57_RS13780"
                     /old_locus_tag="OSZ57_13780"
     CDS             complement(756..1499)
                     /locus_tag="OSZ57_RS13780"
                     /old_locus_tag="OSZ57_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            1925..2908
                     /locus_tag="OSZ57_RS13785"
                     /old_locus_tag="OSZ57_13785"
     CDS             1925..2908
                     /locus_tag="OSZ57_RS13785"
                     /old_locus_tag="OSZ57_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181107.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            <2928..3217
                     /locus_tag="OSZ57_RS13790"
                     /old_locus_tag="OSZ57_13790"
                     /pseudo=""
     CDS             <2928..3217
                     /locus_tag="OSZ57_RS13790"
                     /old_locus_tag="OSZ57_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            3764..3913
                     /locus_tag="OSZ57_RS13795"
                     /old_locus_tag="OSZ57_13795"
     CDS             3764..3913
                     /locus_tag="OSZ57_RS13795"
                     /old_locus_tag="OSZ57_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(4081..4773)
                     /locus_tag="OSZ57_RS13800"
                     /old_locus_tag="OSZ57_13800"
     CDS             complement(4081..4773)
                     /locus_tag="OSZ57_RS13800"
                     /old_locus_tag="OSZ57_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            4883..5077
                     /locus_tag="OSZ57_RS13805"
                     /old_locus_tag="OSZ57_13805"
     CDS             4883..5077
                     /locus_tag="OSZ57_RS13805"
                     /old_locus_tag="OSZ57_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            6194..6811
                     /gene="cadD"
                     /locus_tag="OSZ57_RS13810"
                     /old_locus_tag="OSZ57_13810"
     ARG             6195..6808
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6830..7177
                     /locus_tag="OSZ57_RS13815"
                     /old_locus_tag="OSZ57_13815"
     CDS             6830..7177
                     /locus_tag="OSZ57_RS13815"
                     /old_locus_tag="OSZ57_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            7334..7426
                     /locus_tag="OSZ57_RS13820"
                     /old_locus_tag="OSZ57_13820"
     CDS             7334..7426
                     /locus_tag="OSZ57_RS13820"
                     /old_locus_tag="OSZ57_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(7598..8569)
                     /locus_tag="OSZ57_RS13825"
                     /old_locus_tag="OSZ57_13825"
     CDS             complement(7598..8569)
                     /locus_tag="OSZ57_RS13825"
                     /old_locus_tag="OSZ57_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            8693..9112
                     /locus_tag="OSZ57_RS13830"
                     /old_locus_tag="OSZ57_13830"
     CDS             8693..9112
                     /locus_tag="OSZ57_RS13830"
                     /old_locus_tag="OSZ57_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(9638..10483)
                     /gene="blaZ"
                     /locus_tag="OSZ57_RS13835"
                     /old_locus_tag="OSZ57_13835"
     ARG             complement(9642..10483)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            10590..12347
                     /gene="blaR1"
                     /locus_tag="OSZ57_RS13840"
                     /old_locus_tag="OSZ57_13840"
     ARG             10591..12344
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            12337..12717
                     /gene="blaI"
                     /locus_tag="OSZ57_RS13845"
                     /old_locus_tag="OSZ57_13845"
     ARG             12338..12714
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            12981..13559
                     /locus_tag="OSZ57_RS13850"
                     /old_locus_tag="OSZ57_13850"
     CDS             12981..13559
                     /locus_tag="OSZ57_RS13850"
                     /old_locus_tag="OSZ57_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(13782..13967)
                     /locus_tag="OSZ57_RS13855"
                     /old_locus_tag="OSZ57_13855"
     CDS             complement(13782..13967)
                     /locus_tag="OSZ57_RS13855"
                     /old_locus_tag="OSZ57_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001037130.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTILI
                     LLTSIITVVSVNDEDT"
                     /name="hypothetical protein"
     gene            14247..14921
                     /locus_tag="OSZ57_RS13860"
                     /old_locus_tag="OSZ57_13860"
     CDS             14247..14921
                     /locus_tag="OSZ57_RS13860"
                     /old_locus_tag="OSZ57_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105991.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(14953..15582)
                     /locus_tag="OSZ57_RS13865"
                     /old_locus_tag="OSZ57_13865"
     CDS             complement(14953..15582)
                     /locus_tag="OSZ57_RS13865"
                     /old_locus_tag="OSZ57_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834602.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_267934346.1"
                     /translation="MKVLIVEDDLVIGKMLAEELKKWQLEPILIEGFNHVMDAFNTNAP
                     ELVLLDINLPAFNGYHWCQEIRKVSTVPIIFISSRNDSMDQVMAMQMGADDFIEKPFNM
                     TVTITKIQALLRRTYDFTTQVNNLTVDDCELLLDAAKLKFNEETIDLTHTELQIMHSLF
                     QNKGKFVSRNALIEKCWESEHFIDDNTLAVNITRLRKKVLLQSKKI"
                     /name="response regulator transcription factor"
     gene            complement(15722..16396)
                     /locus_tag="OSZ57_RS13870"
                     /old_locus_tag="OSZ57_13870"
     CDS             complement(15722..16396)
                     /locus_tag="OSZ57_RS13870"
                     /old_locus_tag="OSZ57_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            16870..18336
                     /gene="msr(A)"
                     /locus_tag="OSZ57_RS13875"
                     /old_locus_tag="OSZ57_13875"
     ARG             16871..18333
                     /name="msr(A)"
                     /gene="msr(A)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(A)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            18435..19334
                     /locus_tag="OSZ57_RS13880"
                     /old_locus_tag="OSZ57_13880"
     CDS             18435..19334
                     /locus_tag="OSZ57_RS13880"
                     /old_locus_tag="OSZ57_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_267934347.1"
                     /translation="MTRHNEIIKCAEKYQLHTQPQTISLNESGLDFQVAFGKDKHGVEW
                     VLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATIDPE
                     IQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRRMNK
                     VKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDWTEAT
                     HSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIAEFAME
                     SGESAYETMALKELGMKE"
                     /name="Mph(C) family macrolide 2'-phosphotransferase"
     gene            19820..20428
                     /locus_tag="OSZ57_RS13885"
                     /old_locus_tag="OSZ57_13885"
     CDS             19820..20428
                     /locus_tag="OSZ57_RS13885"
                     /old_locus_tag="OSZ57_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     ARG             18436..19331
                     /name="mph(C)"
                     /gene="mph(C)"
                     /gene_name="Mph(C) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             16871..18336
                     /name="msrA"
                     /gene="msrA"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic; streptogramin B antibiotic"
                     /identity="98.36"
                     /coverage="100.0"
     ARG             18436..19334
                     /name="mphC"
                     /gene="mphC"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="98.33"
                     /coverage="100.0"
     ARG             complement(9639..10483)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          3274..3421
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(757..1499)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1926..2908
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.492"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3040..3209
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 atgaatttaa ttccttttat tattatttta attttttctt gtttatggac ggctataact
       61 aacaaatatt taccttcaga aacattagat aaaaaaggac aacaagatcg atacgatgaa
      121 agacaacgta aaatgtttat tgaaatactg gctaaaagtt ttatttggat tgtatattgt
      181 atgttattta cactagtgct taaatttaca ggtttgtcac caagtattga aggatcttgg
      241 ttttctcaat atccagaaat tttctttata attgtagcac tatttcttat gctgtttaat
      301 tactatacta caaataaaaa atatacatct aaaggttaaa ttaatgaaaa atagaattag
      361 agaatataga aaacaaaaag gaatttctca agaaacttta agtaaagaat taaaagtgtc
      421 taggcaaact attaatgcaa tagaaaacaa taaatatgat cctacattga cattagcatt
      481 taagttggca agattgtttg atacaactgt ggatgaatta ttttcttaac cattcattta
      541 tcacaaaaag agagtgcgaa ttcgcactct ctttttgtgt agtagtacta ttaaattttg
      601 tctcaataac gaacgtttat aacgcaatca tgatgagagt attaatacag ttaattattt
      661 gaaatacttt agtttacata atcgatgatt accagaagtt ctcactatat aaaagacact
      721 aaaattttat aacttttagt gcctaatcaa ttattttagc taccaaacaa acgactccaa
      781 aacccctttt taggttccgt ttcttcactc ttttcttcta tatcactatc attgtcattt
      841 agattagctg gaacgtcttc tttttttgct tcttcacctt gtgtactttc gtcgctttga
      901 cttccagata tatccttata ttgaatctct gtttctttag tttcttcctg atctctgtta
      961 atatttacag gattagtagt atatgttttt tcttgtgcat caacgttttg tctagtgtta
     1021 gtagatgtat caaagggata attaaggtgt ttttcttttt ctaattgatt ttctaatttt
     1081 tgaatctttt tattactctc taatgccaga acttgctgat tttcaagcaa ttttgatctt
     1141 tcttgcaact cttttctgta aaattcaatt gttgtttcat gacgactttc ttgtttttca
     1201 atttgattat ttaaagtatc aatctgattt ttaaggattt ctactatttg agcatcgctt
     1261 ttcgattcaa aattaatatt atccgaccct tttttttgtt tgtcttcagt agtactttca
     1321 aaaccatatt tctttttatt ttcatttact ctttgaatga ttttttctat atcggtatct
     1381 tctttaataa acgaagtgtt ttcctgtttt attgtctcta tattcaatcg cttaatatta
     1441 ttaaaaatcg tttgcttact aacccctaat tcttcactca actttttcac acttttcata
     1501 tcatcaaccc tttataaaat gtttataaac ccttgataaa atattataag cttataatgc
     1561 ttaataataa agtgattaat aaataaaaat aaacatccat caaccttttt atcaagggtt
     1621 tatgaaaggt ttatgatatt tatcaagggt ttataaaccc ttgataaagc tatattttcc
     1681 cttttataag tttatttaaa gtgctataat aaacttaata taacaaaaac cccaactact
     1741 ggtaatagtc ggggtttggt aataaaacgt ttagaaactg tttatttata taattttcga
     1801 tacttgtatt atataatgaa taatatctta gtgcaagcac ctaaatcatt aaataaatgt
     1861 aaagctaaat aaaaacgttt tattacctct taccaaacta aaatatggaa agaggttttt
     1921 tgtcatgcaa aatcaatatt ttacagtgca agaaaattac aaagaaagat tttatcaaat
     1981 ccctaaagta ttctttacca gtgaaaatta taaaaatcta actaatgata tgaaaatagc
     2041 ctatgctatc ttacgtgatc gcttaaattt atctattaaa aatagctggg ttgatgaaga
     2101 tggtaacatt tactttgttt attcaaatga aaagctaatg gaaatcttaa attgtaaaaa
     2161 agaaaagtta acaaaaataa aaaaagggtt agaaaacgat ggtctactca ttcaaaaacg
     2221 acgtggttta aataaaccaa atattctata tttaatgaaa cctattgtca cagaacgtga
     2281 tatttacaaa atagaaaaag aagaaaacga tgttgaacct tacggcgaga aggaagttcg
     2341 aaaatcgaac gtccagaagt tcgaaaatcg aacttccaga agttcgaaaa tcgaacgtcc
     2401 agaagttcga aaatcgaaca ctaatgatac tgactttaat gacattgact ttaatgatac
     2461 tgagaataat gatatgaatg acttgaatga tataaagtac aaaaatgaaa ttagtaatca
     2521 ttcaaatcat tcaaatcaat ttacacataa ttttgatgat aaagaaatgc tattgcaaga
     2581 attcccagaa caactaacta attatttatt aaaatatgat tatagagatt tagaaatcat
     2641 aaaggctgtt attcttaaag caaaaaaatc atttaattca aggcatgaag atatgcatta
     2701 catgttagag gacattgagg atgaaatatt aacttcttta aaaagactta aaaaagctat
     2761 acatgatagg ggagttaaag gccaaaagga aacaatcaag tcaatgcaag cttatttaat
     2821 gcaaacaatt ctaactgaac ttgaagaaac acatgccctt tatatgcgcc gtaaaaacat
     2881 gaaacaatat aatatcttta atcaataatt caaatgattt tataacactc gaagagacat
     2941 caaaatattc cgttaaatca tctgactttt taactgatga cgacgaaaaa aatcaagaaa
     3001 ttgtaagtga tttagaaaaa ggtttgtatc gaaaacgtat gttaagttat ggtggattgc
     3061 ttaaacaaaa gcataaaatt ttaaacttag acgatgccga agatggtaat ttgattaata
     3121 caagtgatga agataaaacg acagacgaag aaaaaaagca cattcaatta ccgcaatttg
     3181 gaatttcgaa aagcaaaatt attatttaag acattagtga taactgatgt ctttttttat
     3241 tgtttttctg taaaaaagtg ctgtcttatt tttgtgacaa atgctgtatg tagtgtcaca
     3301 aaagtgtgac actacagctt tgtatgatat cactttaaaa taacttaaaa cccttggaat
     3361 gtctggcctt gccagatcta tcatttttga atgatagcaa atttccctta tgctcttacg
     3421 gagattttag agttaaatta aaatttctaa gtttttgata agaaaagccc tgcaggaatt
     3481 tagaaaaaca tggggaagtt ttaaagtatt ttatgctaat ttttaaattt gcatgtaaat
     3541 gggcagtatc taaaaaatta gtcactgatt ttgctgaaat ttttgtttgt gaaattagca
     3601 tatatttatt tggctcatat ttgcgtttta agagactgta tagattttta agtacaaaac
     3661 ctatatgatt tgcctctaag tcttcaaaat gcctcttaaa atccaaaata aaagtgttta
     3721 atattgtaat aaggattgct atttttataa aaggagagat atcatgaaga ttaaaacttt
     3781 cgctactttt actcatcgtc aattagataa acaagttaat aggtttttaa agaatgatat
     3841 tcatgtatta gaaattcaaa cacacagtaa ttggttatat atttcagcta ttgtttttta
     3901 tgaggataaa taaggaacat cgtatttatg ccgaaaaaat ttattgatgt tgagaagaac
     3961 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
     4021 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaaacaat tggaataaaa
     4081 tcaacctaca gagcatccag ctgcaaatgc agttccgcaa actgcttcac atacaccgcc
     4141 ggctattgga tttacgattg cccatactcc acagtagtga gcacatgcat aaagtccagc
     4201 catactacaa gcgaatgctt tgccgttcca tttgaatcct gcatatgtta ccggtctgtt
     4261 tttatcaact ttttcagcct tatattcaac gtaatttttg ttaataagta tttttccctc
     4321 tttgttttca gtcatacttc ttttttcggc aatgaatttt gtgatttttt tgttgtagtt
     4381 atcatatatt gtttcagaaa ctacaatttc ttttgttttt tcatttttat aagcgttatt
     4441 agctatgtag ccatatgcat ttttatcatt tgcttctgta gctagatatt ttaatttaga
     4501 atttttgtct tctagcttta tttttttaaa tccttctttt ttcatttgct tttcgaattt
     4561 ttgcatatct tcatttttat tttgcaattg ttctagaaaa cttatttctt tcttggtcat
     4621 ttgtgatact tcgatgtttt tgcttttcaa tttttctttt tgattttcta gtaaagaact
     4681 tttttgttct tcatttgcgt aagcattaga aaaattcgcg ctaaatacca gtaggaacaa
     4741 ggaaattatt gaaaataatt ttataatttt catttgatac ctccaagata ataagatatt
     4801 ttaattgtat aagtaagcgc tttataaaat caatatttat tttaatatat ttataaacat
     4861 atttttgaaa ggagtctatt ttatgaatga aaatttagaa aaatatataa aaagtttgcc
     4921 acttttaggt ttgttaatta gtattttgtt aattgtttta ttctttttta ttttagatgt
     4981 tgatgaagat ttattcgtgg tttttttata ctgtttattg cctttgatag ttaatttgtc
     5041 tatttatggt ttttatgttt ttgttaaaag aaagtaagat tatgaataat tttgttttta
     5101 atttgtatgc tttgaggggg ttggaaaacg gtaggtgtat agattagatt tcttgttttt
     5161 ttgaaacgag tgtgaacgag tttcttcttg tcttgatttt tgataaaaac gccctacagg
     5221 aatttagaaa aacgtgggga agttttaaag aattttatgc taatttttaa tttgcatgta
     5281 aatgggcagt gtctaaaaat agttattgtt tttgctcaaa tttttgtttt tgaaaatagc
     5341 atatatttat ttggctcata tttgcgtttt aaaagcatat ataagtttta gatataaaac
     5401 tatatgaatc acccctaaat ctttaaaatg ccctcttaaa attcaaaata agggcataaa
     5461 aaataaaagc taccccagat aaagtgaggt agctttttct ttcatacggt ctatttattt
     5521 ttctttcgac gccgtatcgt cgatgtaatt caaaaaagtt atgggctata aatctacatc
     5581 actttttcaa gaatgtagta gcaatattca acgcaaatta atttttataa ctgctcggaa
     5641 tatctcaagc cgtttcttta attttgaaat aaaaaaatcg acgaaggtcg attacgtttt
     5701 tgcacatgtc gatttatccg acgtataagt gcgcccttac gggatttaac tagattataa
     5761 cgacgaattt tagacctgta aagcaataac aattaggtct accaggtaga taaagtgtac
     5821 aaagtagaac cgaaaaagtt atttattttg aagatttttc gtagatataa tttgctaata
     5881 aacttcctaa aactgaataa agaaaaatag ttaaactcat gtaatcactc cataattctg
     5941 aagtgtctcc atgtctacgc atagacatgc atttatgtcg agaaaattta ttgtgcgttg
     6001 agaagaaccc ttaactaaac ttgcagacga atgtcggcat agcgtgagct attaagccga
     6061 ccattcgaca agttttggga ttgttaaggg ttccgaggct caacgtcaat aaagcaattg
     6121 gaataaagca cctatgatta tttaaaaata taagttataa tacattcaaa aatatttttg
     6181 aatgaggtgt attatgattc aaacggttgt tgctgctgct gttctttata ttgctacagc
     6241 agttgattta ttagtaattt tattaatatt ttttgctaga gcaaagacta gaaaagaata
     6301 tagagatatt tatgttggtc aatatttagg gtctattatt ttaatattag ttagtttgtt
     6361 tttagctttt gtattaaatt atgttccaga aaagtggata ttaggtttat tgggtttaat
     6421 accaatttat cttggaatta aagtggctat ttatgatgat tgtgaaggag aaaagagagc
     6481 taaaaaagaa ttgaatgaaa aaggattgtc taaattagtt ggtacggttg caattgttac
     6541 gatagcaagt tgtggcgccg ataatattgg tttatttgtt ccgtattttg tgacattaag
     6601 tgttactaat ttattactta ctctgtttgt ctttttaatt ttaattttct tcttggtgtt
     6661 tactgcacaa aaattagcta atattccagg aattggagaa attgttgaga aatttagtcg
     6721 ttggattatg gctattattt atatagcttt aggtttattt attattattg aaaatgacac
     6781 tattcaaaca attttaggat ttatatttta atttagggtg tgattttata tgagttatga
     6841 aaatacttgt gatgtgatct gtgtacatga ggataaagtt aacaatgctt taagtttttt
     6901 agaagatgat aaatctaaga aattacttaa cattttagaa aaaatttgtg atgagaagaa
     6961 attgaaaatc atattatctt tgattaaaga agatgagttg tgtgtttgtg atatttcttt
     7021 gatattgaaa atgagtgttg cttcaacttc acatcattta aggcttttat ataaaaatga
     7081 ggtacttgat ttttataaag atggaaagat ggcatattat tttattaaag acgatgaaat
     7141 aagagagttt ttctctaaaa atcatgaggg tttttgaaac gagtttcttc ttgtcttgat
     7201 actataaaaa acaaagttat ataacataag acgacttatc ggaagtagat atgcttgtat
     7261 attcacagat ggtataataa tagagtcgcc tatctctcag gcgtcaattg acgaagagag
     7321 gaggtgcatt actttgctaa tcattttcgt tcacatcata gcaccagtca tcagtggctg
     7381 tgctgttgcg tattatactt attggcttag taaacgcaac aaataattaa taggcgactt
     7441 tataagccag catcgaaaaa atccccacac tactgcaata gtgtggggat tggtgcatta
     7501 attgttaatc attttcgtta tataaattat aacactggtt agtagagaaa tgcaaaatga
     7561 ttgaattaat agtaaatgta ttaatgaata ctcacactta tacttttaaa atagttcttc
     7621 ctaaatgttg tccttttaaa agctgatcta tcgttttata gaattcttca aatccaattt
     7681 cattgtatag aagtgaattt cctatattcc attcatttgc taacttactc catatatctt
     7741 cacggatatt atgatcaaca tatacagaat caatgccaat taatttaata cctctaagaa
     7801 taaacggtaa tatattagcg ttaagtgaat ttcctgctac atttccacat acagtagcaa
     7861 caccttgata atttagacgt tttaaaccgt ataatgttac attactcccc actgtatcta
     7921 agaaataatc aaaaatacct tttttaagcg gtttattatc atcttcaatt attattactt
     7981 gttttgcacc taattttttt gcgatttcta tttgcttttc ttttcttacg atagcagtga
     8041 tattttcata tccaatttta tgtaaaattt gtatagcaac actgcccaca ccacctgtag
     8101 ccccagacac taaaatttca ggttgtgatt tgatattcat tccatatttt tctagttcaa
     8161 aaattgataa tgctgccgta aaaccggctg tgccatatac catagactct ttaatagaca
     8221 tttcttgggg aataggaaca atccactctc caggcacttg tgcatattct gaaaatccac
     8281 ctgtatgtgt cacgccaaca tcataaccag taactaatac attatcgcct tctttaaact
     8341 tatcattcga acttgattca acaatacctg caaaatctat accaggaatc ataggataat
     8401 ctctaattac accgccttta tattgtaaag ctagcatatc cttataattt attgaagagt
     8461 ataccacttt tactaataca tcacccttcg ataaatgaga ttcatcaact ttttctactt
     8521 tataactaac atttgatgca ctttttctca ctactaccgc attgaacatt tattagcctt
     8581 ctttcacaat tgttttgtag aagtatgttt ttgttgcaaa actattttcc tacaaaactt
     8641 atcatgatat gataagcgag tcaatactaa tgatttaaga gaggttttag ttgtggaatt
     8701 agataattgt ataaattatt tgttgagtac ttctcagaat agagttttta aacattttaa
     8761 aagtttatta agtccctata atattactcc agcagaatat ggtgttttga attgcttgat
     8821 taatagtaat ttgaatacac ctaaagcgat tggtaatact ttaaatttag aaccttcaac
     8881 aatatctgga atattagata agatgtccaa aaaggatcta ataacacgtc aaactgataa
     8941 tgaaaataga agaactgtgc taataaacag tacaaataaa gcaaaagact tatggccaac
     9001 attagaaaag aaagcctatc aacttaataa tcattacttc gctaatttaa ctaatgaaga
     9061 aactgtagtg tttaaaaaga tattactaaa gataaataaa actacgtttt aatttttacg
     9121 ttattaaaaa gttgaagtgc gaattataat cgagtaaata taaatgatag cttagcttaa
     9181 ttatatatta aagccaacta tacttttatt cccgaaaaac ctactatcca aatttctaag
     9241 atactttagc ctctataata aacatattgt aatataattt atgtattatt taggtttacc
     9301 ctaatcgaac aataaaaaat acttgtaaaa tgctattgtc actttgcttg atatatgaga
     9361 attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt
     9421 tcgattgatg aacacctata attaaactat tcatctatta tttatgattt tttgtatata
     9481 caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata ataaagggaa
     9541 aatcagtttt tgatatcaaa attatacatg tcaacgataa tacaaaatat aatacaaact
     9601 ataagatgtt atcagtattt attatgcatt tagaatatta aaattccttc attacactct
     9661 tggcggtttc acttatcaac ttatcatttg gcttatcact tttattgtct ttattcgtaa
     9721 aaatgactaa aacaataggt tcagattggc ccttaggata aacaaaagca acatcatttc
     9781 tagaagcata tgttattgct tgaccacttt tatcagcaac cttatagtct tttggaacac
     9841 catctttaat taaagtgtct ccgtttttat tattaaacat taaatcaagt aagaaatttt
     9901 tatttttttt gcttaatttt ccatttgcga taagtttatt taaagtcttg ccgaaagcag
     9961 caggcgttga agtatctttt ttgctctttg gtgaatagta atttaattct atctcatatc
    10021 taactggatt tgttacttta tctcctagtt cttttagacg ttgtttaact tttttgattc
    10081 caccgatttc ttttataatt ttattgtttg ctgtattatc actatatgtc attgaagcct
    10141 caataagttc ttttaaagtg atatcttttc ctacatattt ttctaaaata ggagaataag
    10201 caactatatc atctttgtta atatgtactt ttttatttaa cttattataa ggtacttgtt
    10261 ctaacaaaat agcactattt atcgcttttg aagtcgaagc ataggcaaat ctcttatctg
    10321 aattaaattt tacttcctta ccacttttag tatttaaagc ataaacacca atatgagcat
    10381 tatatttttt ttctaaatca tttaactctt tggcatgtga actgtttgaa ttacatgcac
    10441 ttaaaactaa agcaattaca attaaaaata ttaacttttt caaaataaac cctccgatat
    10501 tacagttgta atttttataa atcaataata ttacaattgt aatattgatg tcaattgttt
    10561 attataaata tttgaaagaa ggtgtcaaaa tggctaaatt actaataatg agcgtagtaa
    10621 gcttttgttt tatatttcta ttgttagtat tttttaggta catattaaaa cgctatttta
    10681 attatagttt aaattataaa gtttggtatc taactatgct tgctggatta attcctttta
    10741 tcccaattaa attctctttt ataaaattta ataatgtgaa taatcaagcg cccacagttg
    10801 aaagtaagtc acacgatttg aaccataaca taaataccac caaacctatt caagagttca
    10861 caacagatat ccataagttt aattgggatt caattgataa tatctgcaca gttatttgga
    10921 tagttttagt tattatttta agttttaaat ttttgaaagc cttattatat cttaaatatt
    10981 taaagaaaca gtcactttat ctaaacgaaa atgaaaaaaa taaaatagat acgatacttt
    11041 tcaaccatca atataaaaaa aatattgtga ttcgaaaagc agaggctatt caatctccaa
    11101 taactttttg gtatgggaaa tatattattt tgattcctag ttcatatttt aaaagtgtaa
    11161 ttgataaaag actaaaatat ataatactgc atgaatatgc tcatgctaaa aatagagata
    11221 ctttacattt aattatcttt aatatcttct ctattattat gagttacaat ccattagtac
    11281 atattgtaaa aagaaagata attcatgaca atgaagtaga agccgataga tttgtcctta
    11341 ataatattaa caaaaatgaa tttaaaactt atgcggaatc tattatggac tccgtattaa
    11401 aaaccccatt ttccaacaaa aatatattaa gccattcatt taatggtaaa aaatcattac
    11461 tcaaaagtag attaattaat ataaaagaag ccgatctgaa aaagcagtca aaattaattc
    11521 taatttttat ttgtattttt acttttttta tcatgataat tcaaagccag tttttaatgg
    11581 ggcaatcctt aactgattat aattataaaa agcccttaca aagcgattac caaattttag
    11641 atgaaagtaa aaattttggt tcaaatagtg gatctttcgt tatgtacagc atgaagaaag
    11701 acaaatacta tatttataat gaaaaagaaa gtagaaaaag atattctcct gattcaactt
    11761 acaaaattta tttagcactg tttggacttg accgtcatat tataagtgat aaaaattcac
    11821 gtatgtcatg gaatcataaa cattatcttt tcgagtcttg gaataaggaa caagatttaa
    11881 atacagcaat gcaaaattca gttaattggt acttcgaacg tattagtaat caaataccaa
    11941 agaactatac tgcggctcaa ctcaagcaat taaattatgg taatgaaaat ttgggaagtt
    12001 ataaaagcta ttggatggaa gatagtttga aaatatctaa tcttgaacaa gtaatagttt
    12061 ttaaaaatat gatggaacaa aataaccatt ttagtaaaaa agcaaagaat caattatctt
    12121 cttcattatt gattaagaaa aatgaaaagt atgaactgta tgggaaaaca ggtacaggta
    12181 tagtaaacgg gaagtataat aatgggtggt ttgtaggtta cgtaattaca aatcatgata
    12241 agtattattt tgctacacat ttatcagatg gaaacccgtc tgggaaaaat gctgaattaa
    12301 taagtgaaaa aatattaaaa ggaatgggtg ttttaaatga ccaataagca agttgaaata
    12361 tctatggctg aatgggatgt tatgaatata atatgggata aaaaatcagt atcagctaat
    12421 gaaattgtag ttgaaattca aaaatataaa gaagttagtg ataaaacgat tagaacatta
    12481 atcacaagac tatataaaaa agagattata aaacgataca aatcagaaaa tatttatttt
    12541 tactcatcaa atattaaaga agacgatatt aaaatgaaaa ctgctaaaac ctttcttaat
    12601 aaactgtatg gaggggatat gaaaagttta gtgctgaatt ttgcgaaaaa tgaagaatta
    12661 aataacaaag aaattgaaga attgcgagac attttaaatg atattagtaa aaagtaatta
    12721 gaggtatgtt tcaaacataa tacatatgta aaccatactt gactcttatt aaattagttt
    12781 tgttaatgac atttaaaata tattattaaa gatgagagtg tccagaaaat aaccattttc
    12841 tgtacactct tgttttttgt ctaaataagt aagtattaca tagaattatg tgttcaaaaa
    12901 cccaacaaaa aatctatgtt cacaaaagac ctcgtaatca agttatggta cactaaaggt
    12961 gtatatagga ggtctgtatt ttgaaaatag gttatgcaag agtttcaact ggattacaaa
    13021 atttgaattt acaggaggat cgattaaata catatggttg tgaaaagata tttaatgacc
    13081 atatgagtgg ttcaaaaagt aagcgacctg gtttagataa agcaattgaa tttgcgaggt
    13141 caggagatac gattgttgtt tggagattag atagactagg acggaacatg gaggatttaa
    13201 tcacgttagt taatgaactt aacgaacgag gtgtgagttt tcatagttta gaagaaaata
    13261 taacgatgga taaatcaagt tctacaggac aattattatt tcatttattc gcagcatttg
    13321 cagagtttga acgtaattta attttagagc gatcatcagc cggtcgaatt gcagcacgtg
    13381 ctagaggccg atatggaggt agacctgaaa aattaaataa acaagattta aacttactta
    13441 agacacttta tgataatggt acgccaatta aaacaattgc agagcagtgg caagtttcac
    13501 gtacaactat ttatcgttat ctcaataaat tagaggcaaa agagaataaa aacaagtaga
    13561 gcaactaatc tagcttacta taatgaatct actaaataat tttatatatc attaacatac
    13621 aaagactttg attcaatgtc actcgatgta aaagagacgt tctgacagcg tctaaaatct
    13681 cataatattt tcctgttatg agattttaat aataacacaa gaaaaaagcg tacaaattcg
    13741 tacgcttttt taatataata cctgtttcac taatgaacgt tttaagtatc ttcatcatta
    13801 actgaaacta cagtaataat actcgtcaac aatattagta tagttaaaat agaaagactt
    13861 aggactggca taaccgaact aaaattatat ttgaaatata aaagtgctaa tgaagagaga
    13921 aatgtaaaaa taataaatac caaagaccat ttttctaatt tattcattgg cttcacctct
    13981 ttgaactttt atataatcat aggagatatt tttactacga tttataaaaa ataagaagat
    14041 aatcattatt aaagaaacga ttaatataat gttgaatcct atagtatcac ctaattcgtt
    14101 tacacctttg cctaatatcg gagatacaaa gctttgtatt aataatgaaa gagatgccca
    14161 tatagtaaat gaccttccag tgtattcatc ggttctgttg caaagttgaa tttatagtat
    14221 aattttaaca aaaaggagtc ttctgtatga actatttcag atataaacaa tttaacaagg
    14281 atgttatcac tgtagctgtt ggctactatc taagatatgc attgagttat cgtgatatat
    14341 ctgaaatatt aagggaacgt ggtgtaaacg ttcatcattc aacggtctac cgttgggttc
    14401 aagaatatgc cccaatttta tatcaaattt ggaagaaaaa gcataaaaaa gcttattaca
    14461 aatggcgtat tgatgagacg tacatcaaaa taaaaggaaa atggagctat ttatatcgtg
    14521 ccattgatgc agagggacat acattagata tttggttgcg taagcaacga gataatcatt
    14581 cagcatatgc gtttatcaaa cgtctcatta aacaatttgg taaacctcaa aaggtaatta
    14641 cagatcaggc accttcaacg aaggtagcaa tggttaaagt aattaaagct tttaaactta
    14701 aacctgactg ccattgtaca tcgaaatatc tgaataacct cattgagcaa gatcaccgtc
    14761 atattaaagt aagaaagaca aggtatcaaa gtatcaatac agcaaagaat actttaaaag
    14821 gtattgagtg tatttacgct ctatataaaa agaaccgcag gtctcttcag atctacggat
    14881 tttcgccatg ccacgaaatt agcatcatgc tagcaagtta agcgaacact gacatgataa
    14941 attaatggtt agctatattt ttttactttg caacagaacc tttttgcgta aacgtgtaat
    15001 gttgacagca agcgtattgt catcaatgaa gtgttctgat tcccagcatt tttcgattaa
    15061 cgcattacga ctgacgaatt tgcccttgtt ctggaataag ctatgcatga tttgcaattc
    15121 agtgtgtgtc aggtctatgg tttcttcatt aaatttaagc tttgctgcat caagtagtaa
    15181 ctcgcaatca tctactgtta aattattaac ttgagtggtg aaatcatacg tgcggcgtaa
    15241 tagtgcttga atcttagtaa ttgtaacagt catattaaat ggcttctcaa tgaaatcatc
    15301 cgcacccatc tgcatcgcca ttacttgatc catactgtcg ttacgcgaac taataaatat
    15361 aatcggtaca gtagaaactt tacgaatctc ctgacaccag tgataaccat tgaaggcagg
    15421 taaattaata tcgagtaaca caagttccgg tgcattcgtg ttgaaggcat ccattacatg
    15481 attgaaacct tctattaaga tcggttctag ctgccacttc ttgagctctt cagcaagcat
    15541 cttcccgata actaagtcat cctctacgat taatactttc atcatctcac caccacagtc
    15601 gttttttatt attatgacat aaatgagggg aagataagaa ataattctgg cacggttttt
    15661 gttggttctg ttgcaaagta aaaaatatag ctaatcacta atatatcatg tcagtgttcg
    15721 tttaacttgc tagcatgatg ctaatttcgt ggcatgggga aaatccgtag atctgaagag
    15781 acctacggtt ctttttatat agagcgtaaa tacattcaat accttttaaa gtattctttg
    15841 ccgtattgat actttgatat cttgtctttc ttactttaat atgacggtga tcttgctcaa
    15901 tgaggttatt cagatatttc gatgtacaat gacagtcagg tttaagttta aaagctttaa
    15961 ttattttagc cattgctgcc ttcgttgaag gtgcctgatc tgtaactacc ttttgaggct
    16021 taccaaattg tttaatgaga cgtttgataa acgcatatgc tgaatgatta tctcgttgct
    16081 tacgcaacca aatatctaat gtatgtccct ctgtatcaat ggcacgatat aaatagctcc
    16141 attttccttt tattttgata tacgtctcat caatacgcca tttgtaataa gcttttttat
    16201 gctttttctt ccaaatttga tataaaattg gggcatattc ttgaacccaa cggtagatcg
    16261 ttgaatgatg aacgtttaca ccacgttctc ttaatatttc agatatatca cgatagctca
    16321 atgcatatct tagatagtag ccaacggcta cagtgataac atccttgtta aattgtttat
    16381 atctgaaata gttcatacag aagactcctt tttgttaaaa ttatattata aattcaactt
    16441 tgcaacagaa ccgtatttta tgagtacact ctaaattaag gagatgtata atcaatctaa
    16501 aattgtattt ttatactttt gttatataat ctttctactt agagatatat tgtaaaaaaa
    16561 ccaacaaaat gttatattaa cattactatt ttatcgatga aggaggatta aaatgacagc
    16621 ttctatgaga ctcaaataaa cctttttgaa tacaaaacta aattaattca aaaaggcttg
    16681 tccgcaatac acactcatag gatgacttat gtcatttttt atccttcgat aggaataata
    16741 aacgctttca aatactaaaa catcataata aatttttaag tgtttgaaag tgtaatgtga
    16801 atgagacggg agtagcctgt ctcatttttt tgcgtacacg tcctttaaat atacaaggag
    16861 tgtataaata tggaacaata tacaattaaa tttaaccaaa tcaatcataa attgacagat
    16921 ttacgatcac ttaacatcgg tcatctttac gcttaccaat ttgaaaaaat agcacttatt
    16981 gggggtaatg gtactggcaa aaccacatta ctaaatatga ttgctcaaaa aacaaaacca
    17041 gaatctggaa cagttgaaac ggttggcgaa attcaatatt ttgaacagct taacatggat
    17101 gtggaaaatg attttaacac gttagacggt agtttaatga gtgaactcca tatacctatg
    17161 catacaaccg acagtatgag tggtggtgaa aaagcaaaat ataaattagc taatgtcata
    17221 tcaaattata gtccgatatt acttttagat gaacctacaa atcacttgga taaaattggt
    17281 aaagattatc tgaaaaatat tttaaaatat tactatggta ctttaattat agtaagtcat
    17341 gatagagcac ttatagacca aattgctgac acaatttggg atatacaaga agatggcaca
    17401 ataagagtgt ttaaaggtaa ttacacacag tatcaaaatc aatatgaaca agaacagtta
    17461 gaacaacaac gtcaatatga acagtatata agtgaaaaac aaagattgtc ccaagccagt
    17521 aaagctaaac gaaatcaagc gcaacaaatg gcacaagcat catcaaaaca aaaaaacaaa
    17581 agtatagcac cagatcgttt aagtgcatca aaacaaaaag gcacggttga gaaggctgct
    17641 caaaaacaag ctaagcatat tgaaaaaaga atggaacatt tggaagaagt tgaaaaacca
    17701 caaagttatc atgaattcaa tttcccacaa aataaaattt atgatatcca taataattat
    17761 ccaatcattg cacaaaatct aacattggtt aaaggaagtc aaaaactgct aacacaagta
    17821 cgattccaaa taccatatgg caaaaatata gcgctcgtag gtgcaaatgg tgtaggtaag
    17881 acaactttac ttgaagctat ttaccaccaa atagagggaa ttgattgttc tcctaaagtg
    17941 caaatggcat actatcgtca acttgcttat gaagacatgc gtgacgtttc attattgcaa
    18001 tatttaatgg atgaaacgga ttcatcagaa tcattcagta gagctatttt aaataacttg
    18061 ggtttaaatg aagcacttga tcgttcttgt aatgttttga gtggtgggga aagaacgaaa
    18121 ttatcgttag cagtattatt ttcaacgaaa gcgaatatgt taattttgga tgaaccaact
    18181 aattttttag atattaaaac attagaagca ttagaaatgt ttatgaataa atatcctgga
    18241 atcattttgt ttacatcaca tgatacaagg tttgttaaac atgtatcaga taaaaaatgg
    18301 gaattaacag gacaatctct tcatgatata acttaaaaac tatacactaa tttatattga
    18361 atattaggtt catttatatc aatataaaaa atacgttcac aagaaaaata taatagaaat
    18421 aaggatccaa taaaatgact cgacataatg aaattattaa atgtgcagaa aaatatcaat
    18481 tacacaccca acctcaaaca atctcattga atgaatcggg acttgatttt caagttgcat
    18541 ttggaaaaga taaacatgga gtagaatggg ttttgagact accaagaaga cctgacgttt
    18601 ataaacgaac aaaacccgaa aaacaaacgg tagacttctt acagaagaat gtttcatttg
    18661 aaataccgaa gtggaaagta catgcaaaag accttattgc ttacccaaaa cttacaggta
    18721 aacccgcagc cacaatagat ccagaaatac aaaattatgt atgggaaatt gaacacaaac
    18781 cattaccaga aaactttatt aacacattag ctgaaacact cgtagattta cacaacatac
    18841 cagaagaaaa cattaacgtt cagcatataa atatcaaaac catacaagaa ataaaaaatg
    18901 actttcaaag aagaatgaat aaagttaaag aaacttatgg cgtatcagat gaattatgga
    18961 acagatggaa acaatggtta gaaaacgacg aactatggcc tcgacatgcg accatgatac
    19021 atggggactt acatccagga catataatgg tagataacca agcaaacgtc acaggtctca
    19081 tagactggac tgaagcaacc cactccgacc catcaatgga ctttatagga caccatcgtg
    19141 tattcgacga cgaaggatta gagcaactta taacagcata cggtaaagct ggaggtgaaa
    19201 tatggccacg aatgaaagag catataatag aactcaatgc agtattccca atgtttatcg
    19261 ctgagtttgc tatggaatca ggagaatcgg cgtatgaaac gatggcattg aaagagttag
    19321 gtatgaaaga gtagaactta tattaaaaga ggcccatttt aaagacgaag gcaatcctga
    19381 tgaaatttta aaactgcgtt taattagtga tggtctttgg ttttctataa tgtattaaat
    19441 atctggacat ggaacaaatg caacaaatgg aaagaatcgt tttcgaaatt tctaactcct
    19501 taaatcagga ggataaataa acggtcattt tcattgaaag cacttatata aatttcatca
    19561 attttaatac ctaccgtaaa tacatttcat aagtattaaa cagatgatta aaatttttag
    19621 ttaatttaaa tatttatgat agagatgacc ttcaaatgaa cggttctgtt acaaagaaaa
    19681 aatctatatt tatttttagt aagttttaat tatagatatg tacactttga gtacacccta
    19741 accatacaaa aagaattgtt gaaattataa caattaaaac gtatgattag ggtgtatatt
    19801 tattatggag tgaagatgaa tgattatagg ttatgcgaga gtatcatcga tagatcaaaa
    19861 tttagaaaga caattggata atttaaagac gtttggtgtg gagaaaatct ttacagagaa
    19921 acagtcggga aagtcagtgg aaaatagacc tgtatttcaa gaagcactca attttgtcag
    19981 aatgggagat cgattcgttg tggaatctat tgatcgttta ggacgtaact atgatgaaat
    20041 tattgaaact gtgaattatt taaaagaaaa agacgttcag ttaatgatta ccagcttacc
    20101 tatgatgaat gaagtaattg gcaatccatt actggataag tttatgaaag atttaatcat
    20161 tcaaatatta gcaatggttt cagaacaaga gagaaatgaa agtaaacgtc gacaagcgca
    20221 agggattcaa gttgcgaagg aaaaaggcgt atataaaggg cgccctttac tttattcacc
    20281 aaatgctaaa gacccacaga aacgtgttat ttatcatcgc gtggtggaaa tgttagaaga
    20341 aggcaaagcg attagtaaga ttgcgaaaga agttaatatc acaagacaaa cagtttatag
    20401 aattaaacat gataagggat tatcttgata aattgattgt caatgcaaat gagcgtctct
    20461 cacagtgtct caaattccat aacatattat tgaattgaga ttttaataat tatacaagaa
    20521 aaaagagtac gagttcgcag ctttctatat agtaattgtt ttactaataa ggtgttttaa
    20581 aatatattat aatgtaaaaa gtatttgaca tttgtatcaa aaatatttac actcataaat
    20641 gtcaaatact ttttacattt atgaggtgtt ata
//
