LOCUS       NZ_CP113220             4822 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SCLC9-2 plasmid pSCLC9-2_5, complete
            sequence.
ACCESSION   NZ_CP113220
VERSION     NZ_CP113220.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31843362
            Assembly: GCF_026626245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,952
            CDSs (total)                      :: 5,825
            Genes (coding)                    :: 5,671
            CDSs (with protein)               :: 5,671
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 62 of 154
            Pseudo Genes (incomplete)         :: 90 of 154
            Pseudo Genes (internal stop)      :: 22 of 154
            Pseudo Genes (multiple problems)  :: 19 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113220.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCLC9-2"
                     /isolation_source="environment"
                     /db_xref="taxon:573"
                     /plasmid="pSCLC9-2_5"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            join(4745..4822,1..291)
                     /locus_tag="OT489_RS29675"
                     /old_locus_tag="OT489_29670"
     CDS             join(4745..4822,1..291)
                     /locus_tag="OT489_RS29675"
                     /old_locus_tag="OT489_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290306.1"
                     /translation="MKTTSEFARDAQKWDDRELGASENHVEVASMADMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAERKQALRRELDKLNEREEIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            347..475
                     /locus_tag="OT489_RS29680"
                     /old_locus_tag="OT489_29675"
     CDS             347..475
                     /locus_tag="OT489_RS29680"
                     /old_locus_tag="OT489_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072201952.1"
                     /translation="MSLKPGPKRIADSTGQPDRRQRDNKKTPGNTDKLKPSKSSSK"
                     /name="hypothetical protein"
     gene            545..808
                     /locus_tag="OT489_RS29685"
                     /old_locus_tag="OT489_29680"
     CDS             545..808
                     /locus_tag="OT489_RS29685"
                     /old_locus_tag="OT489_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072072086.1"
                     /translation="MAAHTSETVKFLQSVSSDQTWWKSRYNPGDSVPYSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(1539..1802)
                     /locus_tag="OT489_RS29690"
                     /old_locus_tag="OT489_29685"
     CDS             complement(1539..1802)
                     /locus_tag="OT489_RS29690"
                     /old_locus_tag="OT489_29685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290305.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLARQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYSMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     gene            complement(2530..2931)
                     /locus_tag="OT489_RS29695"
                     /old_locus_tag="OT489_29690"
     CDS             complement(2530..2931)
                     /locus_tag="OT489_RS29695"
                     /old_locus_tag="OT489_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064175096.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2931..3122)
                     /locus_tag="OT489_RS29700"
                     /old_locus_tag="OT489_29695"
     CDS             complement(2931..3122)
                     /locus_tag="OT489_RS29700"
                     /old_locus_tag="OT489_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3533..3646
                     /locus_tag="OT489_RS29765"
     CDS             3533..3646
                     /locus_tag="OT489_RS29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            4255..4548
                     /locus_tag="OT489_RS29705"
                     /old_locus_tag="OT489_29700"
     CDS             4255..4548
                     /locus_tag="OT489_RS29705"
                     /old_locus_tag="OT489_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290307.1"
                     /translation="MTIVISKEIEEKLTTKHKVTKKEVHESLANLNGNLLIDNREQHKT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNEIERKIYATHGKKTT"
                     /name="hypothetical protein"
     Typing          3171..3646
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 gaagtagcat cgatggctga catgtcagct ttcaacgatg cactatcact gcaagccatc
       61 tcaataagat tacagaaaaa tcttgtaagc gatttaaaat cgatagctga aagctatggt
      121 ataggttatc aaccaatggt tagagacctt ctgcaacgat ttgttattgc agaaagaaaa
      181 caagcattac gtagagagtt ggacaagctc aatgaacgag aagaaataca tcagcaggag
      241 gacacagttc cagttgatga attcattgaa agcataagaa aacaagcatg aaagagctta
      301 ccatctagat agaaaaaatt tcatcgcgtt tgaaaggagg attatcatga gtcttaaacc
      361 tggtccaaaa agaatcgcag attcaacagg gcaacctgat agacgccaac gtgacaataa
      421 aaaaacaccg ggaaatactg ataaattaaa acccagtaaa tcctcaagta aatgaatcag
      481 ttaaaagccg ccttttaggc ggcttttcat tgtcagtcct ataaattcag aaaaggaaag
      541 ctaaatggct gcacatacca gcgaaacagt gaagttttta caaagcgtca gtagcgatca
      601 aacatggtgg aagtcacgat ataatccagg agacagcgtt ccctattctg gaatttatcg
      661 atgtacagtt tgcggaaaag agatcaccag taacggcagc gacccgttcc caccacaaaa
      721 ccaccatcag cacagtcagc aacaagcaat aaaatggcaa ttgattgtca gaacggacac
      781 taaaggtgac cgtttcggaa tcaaatgatt gagagccctc cggggctttt ttttgcacct
      841 gaagctcgaa gatatttacc ataacatccc agccccgcac ttcttgtatg ctaacaatcg
      901 ttacgctgca tgagcgcacg aacgagatgc atgagtcttt ctcgccctcc aaaaaagtta
      961 aacaaaaaaa acaaatcaga ttaagtcatt gtatttaatg cattaattaa aatttacagc
     1021 ctttagataa tagaaaaata aattaaaaat caatcactta aaaaaataca caatttactt
     1081 tacttgttta gtttgtcagt tattataaat gaatattagt tactgatatt aatggtggtt
     1141 tttcatggcg gttgttgtta tctatcgggc taaatgcatg gcttgtcggc atgaatggac
     1201 tagtaggagt gggtttggaa caccgaacta ctgccctaaa tgtcgttcaa aattaattag
     1261 ttgcagtgca attaagtagg tcaaaatgac atcaaaacac aaagggttat gtcctttttg
     1321 taaagctgaa gttatacctc gtgtaattca agaaaaccat gtacgacgag ataagtgcga
     1381 atgccctgag tgcaaagaag caatttacat atgtcgcgct ccggggtgtg agaattacgc
     1441 taagggtggt cgtttgtatg acgatgagtt gtgtccgtca tgtacatcag gtattggcgg
     1501 cggagtcggc acattaggca tgatcgcctt gggtgcagtc atatccacgt ttgttggtaa
     1561 aaaaatggag tgatgaagaa tgtctggaaa cctcggtctg cctcatacat gcccatgctg
     1621 taacgtaaca actgcaaata cagcaggaca gctctcagca ctgttcggct taagaactgt
     1681 accctcagga gcaaccaacc agagttggtg tcgaaaatgc cgttctgccg cgccaaaaaa
     1741 atgaaaaagc cgcctatcca ggcggctttt ttgcttctgc agatatcatt tagatttacc
     1801 ataacgtctt cttgtacgca ccgcaaaaac ggcctcaagg cgtttctgac gggaaaccgg
     1861 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     1921 catgcatgag tatgcacgca agtgcatagc gcgaaaattc acgccattca gccagggtaa
     1981 gtcctgtttc cggcgttctt cctatccgaa aacggtgaac ggggacgcaa tttattgcgt
     2041 ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcacgttc aggaatcggg
     2101 ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattca
     2161 ccggcgattc gaatcgtgaa ttgaatcaag agtcaccatg aacaaaaaaa acccagagtg
     2221 atgaattgaa tcaagagtcg attcatcata acaatatgat aataaagcgt gattcacata
     2281 tgaatcgtgg gcaattcgtc agcgattcgc catagagttg tccacgactt ggatctgcac
     2341 tttgtaacac atcaccgcac atttattttt cattaaccca ttgttgtaaa taaggaatct
     2401 gcaaaatttt aattttgcgt ggggtgtggg tatttttagt ggtgaggagc gttcagagat
     2461 ctgacgattt ttgggaaggt tcaggaactc cccgggatgg ccggagagga aaactgaagc
     2521 atgacggggt caggattttt tccagaattt ccaccatggc gtcggggatt cagttaattt
     2581 tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcctt
     2641 ggacaaaagt aaggtgttca ggtgttcaat ctcctgtttc agatgacgaa tcacctgttc
     2701 actctggtca gcagaggctg tatcacgctg ttcagagacc tgtccagaat ggtgttcacc
     2761 cttcggttgc tcaaagggac caaagacacg taaaagctct gaagtatcta ccatttttgc
     2821 attgcgctca ccactacagg tggacaggtc acctgtttta attaacctgt gcagtgttgt
     2881 cctgctctta cccgtcagtc gggcagcctc actaatagac actctggcca tcagttacca
     2941 tcctgtaatg catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg
     3001 agtttctcac aaaggtcggc ctcgacatcg agatcaagct cgttaagctt ctccagcagg
     3061 agcagagact gattctggat gaatttagcc atgttcaggg cagttttctg ttgtttcgtc
     3121 ataaggggcc tcagggcgtc agtgctcagt gtagggatgt tctcttttcc gcttcctcgc
     3181 tcactccgtc gctacgctcc tgccgttcgg ctgcggcgag cggacgggac acacagaaga
     3241 gtcatcgcga taaccaggaa gaaaagagcc tgtggaaaag gccacggcgt tgccgttttt
     3301 ccataggctc cgcccccctg acaagcatca cagaaacgga cgctcaaatc agcggtggcg
     3361 aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct cgtgcgctct
     3421 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttatctcat
     3481 tccacgcctg acactcagtt ccgggaaggc agttcgctcc aagctggact gtatgcacga
     3541 accccccgtt cagtccgact gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3601 ggaaagacat gcaaaagcgc cactggcagc agccactggt aactgattta gaggagatag
     3661 tcttgaactt atgcgccggt taaggctaaa ctaaacgaac agttttggtg actgtgctcc
     3721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaaccgc
     3781 cttgcagggc ggttttttcg ttttcacagc agtgagatta cgacgactaa cacatgatct
     3841 caagaagatc ctcagcctaa gattgtgcct ttttcttttt gctatctgca gcacctttaa
     3901 cacgacaaac tctacaccac gtctgaggag ctacgttgcc gcgtagattt ctaaaaccaa
     3961 atagagcgtc aacctcttga taggtcaaag ctacgactgg gttcaatcga taatcatagt
     4021 cagaacaatg acatgaatga ggcaatgcta ttgtattgct cataacttct tctatatcta
     4081 tctttcataa ccaatatcaa cggaccaaag gctgcgaaga agcaatcgaa acagcctatg
     4141 tatatacaca tagaaaatga aaacacctta acaatcaagc catggattga caaatgtaaa
     4201 ttttgtatat acaatgaatt caatttcaca acaaaggccg aggcatcatg aaatatgaca
     4261 attgtaatca gtaaagaaat tgaagaaaaa ctaaccacaa aacataaagt aactaaaaaa
     4321 gaagtacatg aaagccttgc aaaccttaac ggtaacctgt tgatagataa cagagaacaa
     4381 cacaaaacca atcctccaac caactggttt atcgctgaaa ctaaccagcg tagagagttg
     4441 aaagtgtgtt tcatgattat cgatggcgac attcatatca aaacagcctt cgaaccgaac
     4501 gaaatagaac gtaaaatcta tgctacgcat ggaaaaaaga cgacataaca gcgctaaata
     4561 agtaattgtt ttgcaaggcc ttaccatgta agaccttgca aaaatcttac atcagcgaaa
     4621 gaactaaaat gaaaatgaag ctgaacctga tttctcaaca tagaagttcc tttcggaact
     4681 agataaagca taaatattgc agttaatcct gatctgcagg aaacagcacc tagaggtcta
     4741 tacaatgaaa actaccagtg aattcgcacg tgatgcacaa aaatgggatg atcgagagct
     4801 cggcgcctct gagaatcacg ta
//
