LOCUS       NZ_CP113219            21381 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SCLC9-2 plasmid pSCLC9-2_tmex_21k,
            complete sequence.
ACCESSION   NZ_CP113219
VERSION     NZ_CP113219.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31843362
            Assembly: GCF_026626245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 21381)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21381)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,952
            CDSs (total)                      :: 5,825
            Genes (coding)                    :: 5,671
            CDSs (with protein)               :: 5,671
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 62 of 154
            Pseudo Genes (incomplete)         :: 90 of 154
            Pseudo Genes (internal stop)      :: 22 of 154
            Pseudo Genes (multiple problems)  :: 19 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113219.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21381
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCLC9-2"
                     /isolation_source="environment"
                     /db_xref="taxon:573"
                     /plasmid="pSCLC9-2_tmex_21k"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            join(19065..21381,1..818)
                     /locus_tag="OT489_RS29575"
                     /old_locus_tag="OT489_29570"
     CDS             join(19065..21381,1..818)
                     /locus_tag="OT489_RS29575"
                     /old_locus_tag="OT489_29570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease subunit
                     TMexD1"
                     /protein_id="WP_118842627.1"
                     /translation="MPLFFIRRPNFAWVVALFISLGGLLVIPFLPVAQYPNVAPPQITV
                     TATYPGASAQVLTDSVTSVIEEELNGAKNLLYFESTSNANGIAEITVTFQPGTDPELAQ
                     VDVQNRLKKAEARMPQAVLTLGIQTEQATAGFLLIYSLRYKDGDKNANTTALADYAVRN
                     VNNEIRRLPGVGKLQFFDSEAAMRVWIDPQKLVGYGLSIDDVNNAIRTQNVQVPAGAFG
                     STPGSSEQELTATLTVKGTLDNPEEFAAIVLRANQDGSRLTLGDVARIEVGSQDYNFGS
                     RQDGKPAVAAAVQLSPGANAIQTAEAVKQRLTELSANFPDNVEFSVPYDTSRFVDVAID
                     KVIMTLIEAMVLVFLVMFLFLQNVRYTLIPSIVVPVCLLGTLTFMYLLGFSVNMMTMFG
                     MVLAIGILVDDAIVVVENVERIMAEEGLAPVPATIKAMGQVSGAIIGITLVLSAVFLPL
                     AFMAGSVGVIYQQFSLSLAVSILFSGFLALTFTPALCATLLKPIPVGHHEKTGFFGWFN
                     RKFTSLTSRYTKLNDKLVPRAGRVMFIYLGVVVLMGFLYMRLPESFVPVEDQGYMIVDI
                     QLPPGATRERTSAAGGELESFLMAREAVQTTFLVLGFSFSGMGENAAIAFPLLKDWSER
                     DSSQSPEAESAAVNQHFANLDDGAIMAVPPPPVEGLGNSGGFALRLQDRAGLGRDALLA
                     ARDEVLGKVNGNPKFLYAMMEGLAEAPQLRLVIDREQARTLGVSFEAISSALSTAFGSS
                     VINDFANAGRQQRVVVQAEQAERMTPESVLRLHVPNDSGSLVPLSAFVTTSWEEGPVQV
                     ARYNGYPSIRIAGDAAPGVSTGEAMLELERIAAELPEGIGYEWTGLSYQERVASGQATM
                     LFALAITVVFLLLVALYESWAIPLTVMLIVPVGALGAVLAVTAIGLPNDVYFKVGLITV
                     IGLAAKNAILIVEFAKDLWEDGYSLRDAAVEAARLRFRPIIMTSMAFMLGVVPLAIATG
                     AGAASQRALGTGVLGGMLSATMLGVIFVPIFFVWVLSLLRTKPQQTDNHPLHKAE"
                     /name="multidrug efflux RND transporter permease subunit
                     TMexD1"
     gene            823..2256
                     /locus_tag="OT489_RS29580"
                     /old_locus_tag="OT489_29575"
     CDS             823..2256
                     /locus_tag="OT489_RS29580"
                     /old_locus_tag="OT489_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit TOprJ1"
                     /protein_id="WP_003152694.1"
                     /translation="MTSHFMLRRALLPLAIAALAGCSLAPTYERPQAPVASHWQAADAE
                     GARAQALDWQTFIVDADLRRAVDTALSNNRSLRQALLDIEAARAQYRIQRADRLPSINA
                     NASGNRQRLPADLSQTGRSEVTSNYQVGLGLAEYEVDLFGRVRNLSEAALETYLATEEA
                     TRATQISLVAEVIQAYLTRDGALRRMALVEQTLDSRMASLELVSQRRAAGAATALDYQE
                     AVGLAEQARAERESTERQLRQADNALVLLLGTPDAARLLPATPRDDLMVLQDIAPGTSS
                     ELIERRPDILASEHRLKARNADIGAARAAFFPRISLTGSVGSSSAELSGLFDGGSRAWS
                     FAPTLSLPIFAGGRNRANLDLAEVRQDAAVADYEGTIQTAFREVADALAATDTLRREEA
                     ARQALAGSSEAAMALAKARYEGGVDDYLRYLDAQRSTFSNQTTLIQISTERQIALVDLF
                     RSLGGGWGQTEPMAGIGAE"
                     /name="multidrug efflux transporter outer membrane subunit
                     TOprJ1"
     gene            complement(<2701..2916)
                     /locus_tag="OT489_RS29585"
                     /old_locus_tag="OT489_29580"
                     /pseudo=""
     CDS             complement(<2701..2916)
                     /locus_tag="OT489_RS29585"
                     /old_locus_tag="OT489_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            3042..3710
                     /locus_tag="OT489_RS29590"
                     /old_locus_tag="OT489_29585"
     CDS             3042..3710
                     /locus_tag="OT489_RS29590"
                     /old_locus_tag="OT489_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187120.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_023434504.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDTLRAAGVERDNIYDDLASGGRDD
                     RPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQIDTT
                     TASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQAAMA
                     QRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLTSQLVSFRKLSHLRASSEN
                     "
                     /name="recombinase family protein"
     ARG             4107..4525
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             3723..4522
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            4525..5361
                     /locus_tag="OT489_RS29600"
                     /old_locus_tag="OT489_29595"
     CDS             4525..5361
                     /locus_tag="OT489_RS29600"
                     /old_locus_tag="OT489_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<5467..>6141)
                     /locus_tag="OT489_RS29605"
                     /old_locus_tag="OT489_29600"
                     /pseudo=""
     CDS             complement(<5467..>6141)
                     /locus_tag="OT489_RS29605"
                     /old_locus_tag="OT489_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903 family transposase"
                     /name="IS5-like element IS903 family transposase"
     gene            complement(6331..7776)
                     /locus_tag="OT489_RS29610"
                     /old_locus_tag="OT489_29605"
     CDS             complement(6331..7776)
                     /locus_tag="OT489_RS29610"
                     /old_locus_tag="OT489_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_224715715.1"
                     /translation="MPAFEWVHVQLHQQKGMISLSPPTICNSAKSGRSWMGLVKNRCKN
                     GDHYWVSAYVTPIAKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFS
                     VKILLLIWGSIISSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERAR
                     NISNNPLSQSLYTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIE
                     SSNVLTVEQQAETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTS
                     QSITALEGEIRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQG
                     RGFAVVADEVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEA
                     ATALDGIGQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQS
                     AKSVAQLTSALSELAKQFWEKRG"
                     /name="methyl-accepting chemotaxis protein"
     gene            7859..8563
                     /locus_tag="OT489_RS29615"
                     /old_locus_tag="OT489_29610"
     CDS             7859..8563
                     /locus_tag="OT489_RS29615"
                     /old_locus_tag="OT489_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            8696..9940
                     /locus_tag="OT489_RS29620"
                     /old_locus_tag="OT489_29615"
     CDS             8696..9940
                     /locus_tag="OT489_RS29620"
                     /old_locus_tag="OT489_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="WP_227591316.1"
                     /translation="MILLAVAGVSYTFFQRQAAQVAPKAEGDGPPVATVAAPPVRPDGN
                     DYVPPAGQPQMPTDTAQPVETGGQPTEAQQKAAPAAPSEAMQRRLRQIQQAEDKRLAKW
                     DEALNGDASVQGFTTKGGQNGGTQQVAMQQAGGNRVNADELMNRYLAANGQAAGMGGMG
                     GGMPGLGGGGMGGNSMAAENQQDRKQAFLAGTPEAEVYLARQRKGAVAPSQEIKAGWVI
                     PGVMISGINSDLPGQIIGQVREAVYDSATSTQCLIPPGSRLVGTYDSGVTLGQQRALAV
                     WRRIIYPDGSSISIDNMPGADVGGYAGFNDKVNNHYLRIFGSGLLLSVFSAGIQLSQPQ
                     ASNGENYSSSQIIAGSLGQQMGQIGMQMAQRNMNIQPTLEIRPGYEFNIMVTKDIILPT
                     WEGHPMAGSTGKGCN"
                     /name="TrbI/VirB10 family protein"
     gene            10002..10472
                     /locus_tag="OT489_RS29625"
                     /old_locus_tag="OT489_29620"
     CDS             10002..10472
                     /locus_tag="OT489_RS29625"
                     /old_locus_tag="OT489_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KfrB domain-containing protein"
                     /protein_id="WP_000168937.1"
                     /translation="MASTPNSLNPTGPAPKMMPEGSRKVCVLNGSRQLDQVVKGEWMTI
                     KVLPENGLPKGIYQLAEATKPDTGKDGKESAYTGQVLHVDSKAVYQLSGKGIVEHSRSA
                     FADLEAKGDQIVAGRTYTVGYVNGKGTARPGQAASLAQQQQRSASKGGGISL"
                     /name="KfrB domain-containing protein"
     gene            complement(10538..>12061)
                     /locus_tag="OT489_RS29630"
                     /old_locus_tag="OT489_29625"
                     /pseudo=""
     CDS             complement(10538..>12061)
                     /locus_tag="OT489_RS29630"
                     /old_locus_tag="OT489_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            12123..12875
                     /locus_tag="OT489_RS29635"
                     /old_locus_tag="OT489_29630"
     CDS             12123..12875
                     /locus_tag="OT489_RS29635"
                     /old_locus_tag="OT489_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_000602738.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRDL
                     EEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRYLY
                     RAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKTSVD
                     DGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLWLRKG
                     FGFSGGWTVNDQNDLLARLFGLQKVNKA"
                     /name="IS6 family transposase"
     gene            complement(<12986..13291)
                     /locus_tag="OT489_RS29640"
                     /old_locus_tag="OT489_29635"
                     /pseudo=""
     CDS             complement(<12986..13291)
                     /locus_tag="OT489_RS29640"
                     /old_locus_tag="OT489_29635"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /GO_function="GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /name="AAC(3) family N-acetyltransferase"
     gene            complement(13297..14322)
                     /gene="aph(4)-Ia"
                     /locus_tag="OT489_RS29645"
                     /old_locus_tag="OT489_29640"
     ARG             complement(13301..14322)
                     /name="aph(4)-Ia"
                     /gene="aph(4)-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(14551..15327)
                     /gene="aac(3)-IVa"
                     /locus_tag="OT489_RS29650"
                     /old_locus_tag="OT489_29645"
     ARG             complement(14555..15327)
                     /name="aac(3)-IVa"
                     /gene="aac(3)-IVa"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IVa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            15441..16145
                     /locus_tag="OT489_RS29655"
                     /old_locus_tag="OT489_29650"
     CDS             15441..16145
                     /locus_tag="OT489_RS29655"
                     /old_locus_tag="OT489_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(16091..16708)
                     /locus_tag="OT489_RS29660"
                     /old_locus_tag="OT489_29655"
     CDS             complement(16091..16708)
                     /locus_tag="OT489_RS29660"
                     /old_locus_tag="OT489_29655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_219356037.1"
                     /translation="MPLLDEIIGWAGGLRPWQQEALRRIFARAELTQDDIETILRMVRE
                     QEREDATTGGARPFTLDDVPGAGSGATVRLVGVSGLDQVNGFPSGRAFDLAPEGMTIFF
                     GHNGAGKSGYARVFKNACNARHRVEVLPDAFGAATPARLPSADFAILVDGTPETARWVQ
                     NGPAHLHWHCCKVSDEAAFCLIQRPYISKTLLTRRISPRGSP"
                     /name="hypothetical protein"
     gene            complement(17149..17706)
                     /locus_tag="OT489_RS29665"
                     /old_locus_tag="OT489_29660"
     CDS             complement(17149..17706)
                     /locus_tag="OT489_RS29665"
                     /old_locus_tag="OT489_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_118842625.1"
                     /translation="MDLTAADEKLLKALAIAIVDHPRATFKEIAQAAGVSKARLNRFCG
                     TRDNLIEMLLNHGSVVIYRVIADADLEAAPLDALHRLIEGHLIHRELLVFLSFQWRPDT
                     FDLDAGGCRWLPYSETLDEFFLRGQKLGVFRIDIGAPALTEIFSSLIFGLVDAERRGRI
                     ARAGMAALIEQFFLNGACNYQT"
                     /name="transcriptional regulator"
     gene            17886..19049
                     /locus_tag="OT489_RS29670"
                     /old_locus_tag="OT489_29665"
     CDS             17886..19049
                     /locus_tag="OT489_RS29670"
                     /old_locus_tag="OT489_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118842626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit TMexC1"
                     /protein_id="WP_118842626.1"
                     /translation="MNKFREWITFSVISCLVAVTLVGCDKPEEQREEAPAREVDVLSVK
                     TEPFTVFAELPGRIEPVRVAEVRARVAGIVLKRTFEEGADVKAGDVLFQIDPAPFKAAL
                     SRAQGELARAEAQLFQAQAMVRRYEPLVKIDAVSQQDFDNAMAALQSAQADKRSAQANV
                     ETARLDLGYAEVRAPIAGRIGRAQVTEGALVGQGEATLLARIQQLDPVYADFTQPAADA
                     LRLRAAIAEGKVAGASDQPLSLRVDGTDIERKGTLLFTDISVDRSTGQIALRGQFDNPE
                     GVLLPGMYVRVRTPQGLNQNAILVPQRAVQRSADGQASVMLLGEGDTVEVRQVTTGAMQ
                     GSRWQISEGLQAGDKVITSSLAAIRPGAKVIPREQGAAEKAPQSQAQ"
                     /name="multidrug efflux RND transporter periplasmic adaptor
                     subunit TMexC1"
     ARG             17887..19046
                     /name="tmexC1"
                     /gene="tmexC1"
                     /gene_name="multidrug efflux RND transporter periplasmic
                     adaptor subunit TMexC1"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             824..2253
                     /name="toprJ1"
                     /gene="toprJ1"
                     /gene_name="multidrug efflux transporter outer membrane
                     subunit TOprJ1"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4526..5358
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aggccgagcg catgacaccg gaaagcgtcc tgcgcctgca tgtgcccaac gacagcggca
       61 gcctggtacc gctgagtgct ttcgtcacca cgagctggga agaaggcccg gtgcaggtcg
      121 cgcgttacaa cggttacccg tcgatccgca ttgccggtga cgccgcgccc ggcgtgagca
      181 ctggcgaggc gatgctcgaa ctggagcgca tcgctgccga gctgcccgaa ggtatcggtt
      241 acgagtggac cgggctgtcg tatcaggagc gggtcgccag cgggcaggcg acgatgctgt
      301 tcgccctggc catcaccgtg gtgttcctgc tgctggtggc gctctacgag agctgggcga
      361 tcccgctgac ggtgatgctg atcgtgccgg tcggcgcact cggcgcggta ctggcggtga
      421 ctgctatcgg cctgcccaac gacgtgtact tcaaggtcgg cctgatcacc gtgatcggcc
      481 tggcggcgaa gaacgccatt ctcatcgtcg agttcgccaa ggacctgtgg gaagacggct
      541 actcgctgcg cgatgctgct gtcgaagccg cgcgcctgcg tttccgcccg atcatcatga
      601 cctccatggc gttcatgctc ggcgtggtgc cgctggccat cgccactggc gccggcgctg
      661 cgagccagcg cgcactgggc accggggtgc tgggcgggat gctcagcgcg accatgctcg
      721 gggtgatctt cgtgccgatc ttcttcgtct gggtgctgtc gctgctgcgc accaaacctc
      781 agcaaaccga caaccatccc ctgcataaag cggagtaatg cgatgacctc tcacttcatg
      841 ctccgtcgcg ctctgctgcc cctggccatc gccgccctgg cgggttgttc gctggccccg
      901 acctacgagc gcccgcaggc accggtcgcg tcgcactggc aagccgccga cgcggagggc
      961 gcccgtgccc aggcgctgga ctggcagacc ttcatcgtcg atgccgactt gcgccgcgcg
     1021 gtggatacgg cgctgagcaa caaccgcagc ctgcgccagg cgctgctgga tatcgaagct
     1081 gcgcgtgccc agtaccgcat ccagcgtgct gatcgcctgc cttcgatcaa cgccaatgcc
     1141 agcggcaacc gccagcgcct gcctgccgat ctgtcgcaga ccgggcgctc ggaagtgacc
     1201 agcaactatc aggtcggcct cggcctcgcg gagtacgaag tcgacctgtt cggccgcgtg
     1261 cgcaacctct ccgaggccgc gctggagacc tacctggcga ccgaggaggc gacccgtgcc
     1321 acgcagatca gcctggttgc cgaggtcatc caggcctacc tgacccgcga tggtgcgcta
     1381 cgccgcatgg ccctggtcga acaaaccctg gacagccgca tggcctcgct ggagctggtc
     1441 agccagcgcc gtgcagcggg ggccgccacc gccctggatt accaggaagc ggtcggcctt
     1501 gccgaacagg ctagggccga gcgcgagagc accgaacgcc agttacgcca ggcggacaac
     1561 gccctggtcc tgttgctcgg cacgccagat gccgctcgcc tgctgcccgc gacgccccgc
     1621 gacgacctga tggtgctgca ggacatcgcc cccggcacca gctcggaact gatcgaacgg
     1681 cgcccggaca ttctcgccag cgagcaccgc ctcaaggcgc gcaatgccga tatcggtgca
     1741 gctcgcgccg cgttctttcc ccggatcagc ctgaccggtt cggtgggcag ctccagtgcc
     1801 gagttgtccg gattgttcga tggcggctcg cgggcctgga gctttgcccc gacgctgtcg
     1861 ctgccgatct tcgccggtgg ccgcaaccgc gccaacctgg acctcgccga agtgcgccag
     1921 gacgcggcgg tggccgacta cgaaggcacc atccagaccg ccttccgcga agtggccgat
     1981 gccctggccg ccaccgacac cctgcgccgc gaggaggctg cccgccaggc tctggccggt
     2041 tccagcgagg ctgcgatggc cctggctaag gcgcgttacg agggcggcgt ggacgactac
     2101 ctgcgttatc ttgacgccca gcgcagcacc tttagcaacc agaccacact aattcaaatc
     2161 agcacggaac ggcagattgc gctggttgac ctgttcaggt ctctgggcgg tggctggggc
     2221 caaactgaac cgatggccgg gatcggcgct gagtgagcaa agccaggagt cctgcatccg
     2281 aagaccggac tctggctcca tcaccggtga gctactggtc atcgacgggg caatcgtctg
     2341 atcgagaata agactgtctg acgactggca ggcctatgga ttattggtgg cagcagctaa
     2401 cggctctata aagagctgca aaaaatcact gtaacgcatg attgactgac tgcttctggc
     2461 cggtttctgc ctgttgcgtc tctgctgccc actggccaag tcggatgcac gcggtggtca
     2521 gtaccaatgc aattgactgg tcaaatgggt gcaattgcgc aggtatggac ttcaagcccc
     2581 aatttcctgg aaaagaggtt ctttctatgg acatccgaca tcagagattg agtagcggaa
     2641 tgtctgagct gaagtcatgt ccatggctgt tcttctttcc tttacatcga tgacctctgc
     2701 agggatcagt tcgcctggag ccagcacccg gccgggatag cgcaggacac acaattgcag
     2761 ggcgaagccg aacctgttgt gagcgcgccg acgcagcctg atatgcccaa ggtcttcatc
     2821 actcagcgta tagtgcttga gcaaatccgt ctgtgaagtc ggcaagcgca acagcgcgtc
     2881 tttctgccga tcggttagag tgacgcgacg cggcatacat gttccttttt caaaatctga
     2941 tagcgttcaa gacgctttgt ttatgaagct ggttgagata catttccaga ggtcaatgca
     3001 atcgtggccg aagcgccgcc tcaaaccaac gtttgtgata catgctgatc ggatatgccc
     3061 gcgtctccaa agccgatggc tcgcagtctc tcgacctgca gcacgacacc ttgcgcgccg
     3121 caggtgtcga acgggacaat atctatgatg atcttgcttc cggcggtcgt gatgatcgcc
     3181 ctggcttgac tgcctgcctc aagtcattgc gtgacggcga tgtgctggtg gtctggaagc
     3241 tcgatcgcct cggacgatcg cttgcccatc tggtcaacac ggtgaaggag ctgtcagacc
     3301 gcaagatcgg cctgcgggtt ctgactggaa agggcgctca gatcgacacc acgactgcgt
     3361 ccggtcgcat ggtgttcgga atcttcgcca ccttggccga gttcgagcgg gatctgatcc
     3421 gagagcgcac catggcgggt ctcgcctccg cgagagcgcg cggtcgcaag ggcggacgaa
     3481 aattcgcgct caccaaagct caggtgcgtc tcgcgcaagc cgccatggcc cagcgcgata
     3541 cttcagtttc cgatctctgc aaggaactcg gcatcgagcg cgtcactctc taccgatatg
     3601 tcggtcccaa aggcgagctc agagaccatg gaaagcatgt tctcggactt acgtcgcaac
     3661 tcgtttcttt tcgcaagttg agccacctcc gcgcttcatc agaaaactga aggaacctcc
     3721 attgaatcga actaatattt tttttggtaa atcgcattct gactggttgc ctgtcagagg
     3781 cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg cgaaaatcgc
     3841 acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt ggctcaaagg
     3901 tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt
     3961 ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg atttgctcaa
     4021 agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg ttgatcaatg
     4081 tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa
     4141 tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt
     4201 ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg atatggttgt
     4261 ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc ttcaatgcac
     4321 gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt tggcactcat
     4381 gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg ccttcgctgt
     4441 cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg
     4501 attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc tcattggcac
     4561 gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa agtttcattg
     4621 ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa
     4681 ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg gttgctcggt
     4741 cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc tcacatcgtt
     4801 gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc gaagctgtat
     4861 gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg ctttgcagct
     4921 ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg
     4981 gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct acatggcgat
     5041 ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga tcccgtcggt
     5101 ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc tgacagagac
     5161 gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc tcgtgcgctg
     5221 gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct ttccgcagct
     5281 tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc gatcaagcag
     5341 gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg aacacatcaa
     5401 tcttaccgga gaatatcgtt ggccaaagcc ttagcgtagg atttcgccct ctcccgcaaa
     5461 cgaccccata gcctccgcaa cctgaccatc gtagtcacgc agcgtcagtg aacccccgaa
     5521 cagctgtttt acccggtaca tcgccgtttc cgctatcgag cgacggttgt aatctgttgt
     5581 ccatttccac cgcgcattac tcccggtcat tcgctgatta gccactgcac ggttacggtc
     5641 tgcatattca ccgggccagt aacccgcacc ttttcgggga gggataagcg cgctgatttt
     5701 cttacgccgc agttcatcgt gacagagccg ggtgtcgtaa gcgccgtctg ccgatgctgc
     5761 cctgattttt ctgtgagtct gccggataag acccgggaag gcttctgagt ccgtcacatt
     5821 gttcagcgac aggtctgcac agatgatttc atgtgtgttg ctgtcaactg ccagatgcaa
     5881 ctttcgccat atacgacggc gttcctggcc atgctttttg actttccact cgccttcacc
     5941 aaagaccttc agcccggtgg aatcaatcac cagatgcgcg atttcacccc gggtgaacgt
     6001 tttgaaactg acgttaaccg actttgcccg cctgctgaca cagctgtaat ccgggcagcg
     6061 tagcggaacg ttcatcagag aaaaaatgga atcaataaag ccctgcgcag cgcgcagggt
     6121 cagcctgaat acgcgtttaa tgggggaacc gcagaattcg gaaaaaatcg tacgctaagc
     6181 taacggtgtt ctcgtgacag ctctttgact aggctttcta aggccattct gatagccctg
     6241 acttcctgaa aagccatggc taaaatttgt gcggctaaaa gggataaccg atggtaaagt
     6301 aagttatccc tgtcgagata ctgaaaagcg ttatcctcgt ttttcccaaa actgttttgc
     6361 cagttcgctc agagcgctag ttaactgagc gacagatttc gcactttgca aattattctg
     6421 cccggtctgt acattggtat cagcagcatc gcgtatattg ataatactgc ggtttatgtc
     6481 ttcacttact gctccctgct gctcgaccgc agtcgctatt tgcgcgttca tgtcggtaat
     6541 ttcgttaacg cgttggccaa ttccatcaag agctgtagct gcttcctctg cgtgagctac
     6601 actcgtgtgc gcttgccgac tactttgctc catgactgta acagcggatt gcgctcgctc
     6661 ttgtagagcg ctgatcatgc tttgaatatc cgttgtcgat tgctgtgtgc gagcagcaag
     6721 actgcgaacc tcatcggcga caacagcaaa accacgcccc tgctcaccag cacgcgcggc
     6781 ctcaattgct gcgttgagtg ccaacaaatt cgtttgctcg gcgatccctc gtataacgtc
     6841 aagaactttt gatatctcgt tactttgacc ttcaagctca tgaataacct gagtggcttg
     6901 cctaatttca ccttcaaggg cagtgattga ctggcttgtg tgggctacca gacgctggcc
     6961 agatgccgtc tcagtgtctg ctcttccggc cgcatctgca gcatgctgtg cattgctcgc
     7021 aacctcttga atgcttgcca ccatttggtt tactgccgtt gctatttgat ctgtctctgc
     7081 ctgctgctca actgtaagta cattgcttga ctcaatatcc tttagtaggc ctcgggtgtg
     7141 ttcgctaagc cgatttgatg catcacctat gcgacctact atggcgcctg tttcagcttg
     7201 catcattcgt aaagcaaact ctatttggcc aaactcatcg gtgcgcccag tgtagaggga
     7261 ttgacttaat gggttattgg aaatattcct ggctctttca accagtcttc caagaggaga
     7321 gagaatagcc aaaacactaa cagagcttaa gcttcctgac attaaagtgg ctaacaataa
     7381 gctgcttatt gatgtatcag taagcatgcc ggcagccatt gcgcttgata taatactacc
     7441 ccatatgagc aagagtattt tcacggaaaa gctagcagcc aatttcggcc tcgcggcctt
     7501 cccgcttctc aattgagcat ataatttttc cgcagccaaa acctgctcag gttcaggctt
     7561 ggtccttaca gactggtatt caacaatcga accattctta gctattggcg ttacataagc
     7621 acttacccaa tagtggtcgc catttttaca gcgatttttt actagcccca tccatgagcg
     7681 gccagatttg gcactgttgc aaatagtcgg tggtgataaa cttatcatcc ccttttgctg
     7741 atggagctgc acatgaaccc attcaaaggc cggcattttc agcgtgacat cattcggcac
     7801 tgttgcaaat agtcggtggt gataaactta tcatcccctt ttgctgatgg agctgcacat
     7861 gaacccattc aaaggccggc attttcagcg tgacatcatt ctgtgggccg tacgctggta
     7921 ctgcaaatac ggcatcagtt accgtgagct gcaggagatg ctggctgaac gcggagtgaa
     7981 tgtcgatcac tccacgattt accgctgggt tcagcgttat gcgcctgaaa tggaaaaacg
     8041 gctgcgctgg tactggcgta acccttccga tctttgcccg tggcacatgg atgaaaccta
     8101 cgtgaaggtc aatggccgct gggcgtatct gtaccgggcc gtcgacagcc ggggccgcac
     8161 tgtcgatttt tatctctcct cccgtcgtaa cagcaaagct gcataccggt ttctgggtaa
     8221 aatcctcaac aacgtgaaga agtggcagat cccgcgattc atcaacacgg ataaagcgcc
     8281 cgcctatggt cgcgcgcttg ctctgctcaa acgcgaaggc cggtgcccgt ctgacgttga
     8341 acaccgacag attaagtacc ggaacaacgt gattgaatgc gatcatggca aactgaaacg
     8401 gataatcggc gccacgctgg gatttaaatc catgaagacg gcttacgcca ccatcaaagg
     8461 tattgaggtg atgcgtgcac tacgcaaagg ccaggcctca gcattttatt atggtgatcc
     8521 cctgggcgaa atgcgcctgg taagcagagt ttttgaaatg taaggccttt gaataagaca
     8581 aaaggctgcc tcatcgctaa ctttgcaaca gtgcccgccc gactatctgg cgacaccaaa
     8641 aagagggaag ggggttcgcc gcctgaacaa caaaccgttg ttcgtggtcg gtggcgtgat
     8701 cctgctcgct gtggctggcg ttagctacac gtttttccag cggcaggcgg cgcaggttgc
     8761 ccccaaggcc gaaggcgacg ggccaccagt ggcgacggta gcggcccccc ctgttcggcc
     8821 ggacggcaac gactacgtgc ccccggcagg tcagccgcag atgcccacgg acacggcgca
     8881 gccggtcgaa accggcggcc agccgaccga agcccagcag aaggcggccc cggccgcgcc
     8941 ctctgaggcc atgcaacgcc ggttgcggca gattcagcag gccgaagaca aacggctggc
     9001 caagtgggac gaagccctga acggtgacgc ttcggtgcag ggcttcacga ccaagggcgg
     9061 ccagaacggc ggcacccagc aggtggccat gcagcaagca ggcgggaacc gcgtcaacgc
     9121 cgacgagctg atgaaccgct acttggccgc gaacggtcag gccgcaggta tgggcggcat
     9181 gggcggcggt atgccgggct tgggcggcgg cggtatgggt ggcaacagca tggcggccga
     9241 aaaccagcag gatcgcaagc aagcgttcct cgcaggtacg ccagaagctg aggtttatct
     9301 ggctcgccag cgcaaggggg ccgtcgctcc gtcgcaggaa atcaaagccg gttgggttat
     9361 ccccggcgtg atgatttccg gtatcaactc cgatctgccg ggccaaatca tcgggcaggt
     9421 gcgcgaagcg gtgtacgaca gcgcgaccag cacgcaatgc ctgatcccgc cgggttcgcg
     9481 tttggttggc acctacgaca gcggcgtaac cctcggccag cagcgggcgc tcgcggtctg
     9541 gcggcgcatc atctacccgg atggttcttc gatctcgatt gacaacatgc cgggcgctga
     9601 tgtgggcggc tatgctgggt tcaatgacaa ggtgaacaac cattatctgc gcatcttcgg
     9661 cagtggcttg ctgctttcgg tcttctccgc aggcatccag ctttcgcagc cgcaggccag
     9721 caacggcgag aactacagca gctcgcaaat catcgccggg tcgctgggtc agcagatggg
     9781 gcagattggt atgcagatgg cccaacgcaa catgaacatt cagccgacgc tcgaaatccg
     9841 tccgggctac gagttcaaca tcatggtgac gaaggacatt atcctgccga cgtgggaagg
     9901 ccacccgatg gccggaagca ccggcaaggg ctgcaattga tgactaccgg cggccgttcg
     9961 cggccgcttt tttcactgca atggacagga gtacgaaacg tatggcaagc acccctaaca
    10021 gcctgaaccc gaccgggcca gcgccgaaga tgatgccgga aggctcgcgc aaggtctgcg
    10081 tcctgaacgg tagtcgccag ctcgaccagg tggtgaaagg cgagtggatg actatcaagg
    10141 ttctgccgga aaacggactg cccaagggca tctaccaact ggccgaagcc accaagccgg
    10201 acaccggcaa ggatggcaag gaatccgcct acaccgggca ggttctgcac gtcgatagca
    10261 aggcggtcta ccagctttcc ggcaagggca ttgtcgagca ttcgcgctcg gcctttgccg
    10321 atctggaagc caagggcgac cagatcgttg ccggtcgcac ctacacggtc ggctatgtga
    10381 acgggaaggg caccgcccgg cccggccaag ccgccagtct ggcgcaacag cagcagcgca
    10441 gcgcctcgaa gggcggcggc atttcccttt aacgatgaag gtcagcgcca cgactgacca
    10501 ccaaggggtc gtttgcggga gggggcggaa tcctacgcta aggctttggc cagcgatatt
    10561 ctccggtgag attgatgtgt tcccatccga gcggcgaaac atgggccaag agatcgggcg
    10621 atagcagctt tccatcgcgt ttctggtttg caacgacctc gccgagcttc atggtgttcc
    10681 agaagatgat gatggcggcg agcagattca tgccggcgat gcggtaatgc tggccttcgg
    10741 cggaacggtc gcggatttca ccgcggcggt ggaagctgat tgcccgcttc agcgcatgat
    10801 gagcttcgcc tttgttgagc ccgatctggg cacgccgttg gagttcggca tccagaatcc
    10861 agtcgatcat gaacagggtg cgctcgacgc gaccgacttc ccgcagggct gtcgcgagct
    10921 cgttctgccg cggataggag gcgagtttcc gcagaatctg gcttggcgcg acggtcccgg
    10981 cagcaatggt ggcggcgatg cgcaggatgt cgggccaatt gcgctcgatc atggcttggt
    11041 tgacctttcc gccgatcaac gctcgcaggt gcgccggggc ggccgacgga ttgaacgcgt
    11101 agagccgttt ggatggcagg tcgcggatgc gcggagcgaa ccggtagccg agaatggcac
    11161 atgcggcaaa gacgtgatcg gtgaagccgc ccgtgtcggt gaactgctcg cggatatggc
    11221 gtccagcatc gttcatcagc aggccatcga ggatgtaagg cgcttcgctt gccgttgcag
    11281 gaatcacctg ggttgcgaac ggcgcatatt ggtcggagac gtggctatag gctttcaggc
    11341 ccggggtatt gccatatttc gcgttgacca ggttcatggc ctcaccttgc tctgtagcga
    11401 cgaagaactg tccgtcgctc gaagccgacg tgcccatgcc ccagaaccgg gccatgggta
    11461 acgctgcctg tgcctcgacc accatggcca gcgcccggtc ataggcttcg ccctcgacat
    11521 gccaccgtcc aatgcggatc aattcccaga aggtgtgggt gtttgtcgca tccgccattt
    11581 tgcgcaagcc gaggttgatc ccttccgcca agataacgtt cattagcccg atccggtcag
    11641 cgcagggtgc tcctgtgcgc agatgggtga acgcttcggt gaagccggtc gccgcatcca
    11701 cctccagcag gagatcggtg atgcgcgtgg gcgggatctg cttgtagaga tcgagcacca
    11761 gatcttcggc gcctgtcggc gcggcggctt cgagtttctc gatatgcaga acgccgtttt
    11821 caatcgaccc gcccgggatc gtgcctgcgc gagcggcacg gccaagctcg cgcaaccgca
    11881 tgtcgaggcg agcttgccgg tctgccagcc attcctccgg ccgcaatggc acagcgagac
    11941 gaccgccttc cgcgatggat tgtgccggaa cgagtgcgtg tttcagatcg ccatagcgcc
    12001 gggacctagt aagccagaca tctccggagc ggaacgcatg gccttgtcgc aaattcttca
    12061 ggttaactcg atgttgacca tggggagaga agttgccgct cctgatattg cggagcgagc
    12121 cgatgatttt gaataccatt gccgaaaagc tgaagcgcca gtcgaaggat gatttcaagg
    12181 gcaggcattt cgaggcctgg ctgattgtac aggcggttgc ctggtacttg cgctacccgc
    12241 tcagctatcg tgacttggaa gagatgtttc gcgagcgggg cttcgaggtc gatcatagca
    12301 cgatcaaccg ctgggtattg gcttatgcac ctgtcatcga gaaacggctg cggcagtttc
    12361 gtcgacccca ttgcggctcg gtccggattg atgagaccta tgtcaagatc cgcggcaaat
    12421 ggcgctatct gtaccgagcc atcgataagc atggcaatcc ggtggatttc ctgctgaccg
    12481 ctaagcgcga tctcgacgct gccaagcggt tcttccgaaa gatgcttaaa gatgaaccct
    12541 tgttgtcgcc gaacaggatt gggacggacg gggccaacac cttcccgtca acgatcaaaa
    12601 cgtcggttga tgatgggctt ctccatcccg atcccgtgca ttatgtgacc aaacatctcc
    12661 agcaggggat tgagagcgac catttccggg tgaagaagaa catgccgaag atcggcggtt
    12721 tccaatcctt taacacggcg cggcgaacca tcgcgggttt cgaggcgatg ctgtggctgc
    12781 ggaagggctt tggcttttca ggcggctgga ccgtcaatga ccagaatgat ttgcttgcgc
    12841 gcctcttcgg actgcaaaag gttaacaaag catgaaaata ccggccttgg ctgggtgata
    12901 gctgcctcca aaagtgtttg cgacacgccc ggaacaattc agccactgca cccatgttcc
    12961 gagttggtgt ggtttgcgga tcgtatgccg gcatctcgtt gcgcaagatc tgcacccaat
    13021 cggcaggcac gggcggcgat ctccaatctg cgggatcagt cagatcaccc gagtgcgtgg
    13081 gcatgacaat cgtgccctgg ggaccaacac aatccagaag ggcctgaatc actgcgaccg
    13141 gccctcccgc gacccagccg agcgagctta gcgaactgtg gacgagaact gtgccaccaa
    13201 gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc gcgagttgct ggctgaggcg
    13261 ttctcgaaat cagctcttgt tcggtcggca tctactctat tcctttgccc tcggacgagt
    13321 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
    13381 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
    13441 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
    13501 gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc
    13561 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
    13621 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
    13681 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
    13741 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
    13801 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
    13861 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
    13921 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac
    13981 cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
    14041 acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg
    14101 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
    14161 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
    14221 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
    14281 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gcccccggac
    14341 gagcgtctgc tccgccattc gccgtccgcc gtgccaatcg gatcagccgt ccaaatgcgg
    14401 gattttcgtt agtcggaggc caaacggcat tgagcgtcag catatcatca gcgagctgaa
    14461 gaaagacaat ccccgatccg ctccacgtgt tgccccagca atcagcgcga ccttgcccct
    14521 ccaacgtcat ctcgttctcc gctcatgagc tcagccaatc gactggcgag cggcatcgca
    14581 ttcttcgcat cccgcctctg gcggatgcag gaagatcaac ggatctcggc ccagttgacc
    14641 cagggctgtc gccacaatgt cgcgggagcg gatcaaccga gcaaaggcat gaccgactgg
    14701 accttccttc tgaaggctct tctccttgag ccacctgtcc gccaaggcaa agcgctcaca
    14761 gcagtggtca ttctcgagat aatcgacgcg taccaacttg ccatcctgaa gaatggtgca
    14821 gtgtctcggc accccatagg gaacctttgc catcaactcg gcaagatgca gcgtcgtgtt
    14881 ggcatcgtgt cccacgccga ggagaagtac ctgcccatcg agttcatgga cacgggcgac
    14941 cgggcttgca ggcgagtgag gtggcagggg caatggatca gagatgatct gctctgcctg
    15001 tggccccgct gccgcaaagg caaatggatg ggcgctgcgc tttacatttg gcaggcgcca
    15061 gaatgtgtca gagacaactc caaggtccgg tgtaacgggc gacgtggcag gatcgaacgg
    15121 ctcgtcgtcc agacctgacc acgagggcat gacgagcgtc cctcccggac ccagcgcagc
    15181 acgcagggcc tcgatcagtc caagtggccc atcttcgagg ggccggacgc tacggaagga
    15241 gctgtggacc agcagcacac cgccgggggt aaccccaagg ttgagaagct gaccgatgag
    15301 ctcggctttt cgccattcgt attgcacgac attgcactcc accgctgatg acatcagtcg
    15361 atcatagcac gatcaacggc actgttgcaa atagtcggtg gtgataaact tatcatcccc
    15421 ttttgctgat ggagctgcac atgaacccat tcaaaggccg gcattttcag cgtgacatca
    15481 ttctgtgggc cgtacgctgg tactgcaaat acggcatcag ttaccgtgag ctgcaggaga
    15541 tgctggctga acgcggagtg aatgtcgatc actccacgat ttaccgctgg gttcagcgtt
    15601 atgcgcctga aatggaaaaa cggctgcgct ggtactggcg taacccttcc gatctttgcc
    15661 cgtggcacat ggatgaaacc tacgtgaagg tcaatggccg ctgggcgtat ctgtaccggg
    15721 ccgtcgacag ccggggccgc actgtcgatt tttatctctc ctcccgtcgt aacagcaaag
    15781 ctgcataccg gtttctgggt aaaatcctca acaacgtgaa gaagtggcag atcccgcgat
    15841 tcatcaacac ggataaagcg cccgcctatg gtcgcgcgct tgctctgctc aaacgcgaag
    15901 gccggtgccc gtctgacgtt gaacaccgac agattaagta ccggaacaac gtgattgaat
    15961 gcgatcatgg caaactgaaa cggataatca acgccacgct gggatttaaa tccatgaaga
    16021 cggcttacgc caccatcaaa ggtattgagg tgatgcgtgc actacgcaaa ggccaggcct
    16081 cagcatttta ttatggtgat cccctgggcg aaatgcgcct ggtaagcaga gtttttgaaa
    16141 tgtaaggcct ttgaataaga caaaaggctg cctcatcgct aactttgcaa cagtgccaat
    16201 gcaaatgggc cggcccgttc tggacccaac gtgcggtttc cggagtgcca tccaccaaaa
    16261 tggcgaaatc tgctgatggc agtctagctg gggttgccgc cccaaaagcg tcgggcagga
    16321 cctccacccg atgccgagcg ttgcaggcat tcttgaagac gcgggcgtag ccggatttgc
    16381 cggcgccgtt atgaccaaag aaaatggtca tgccctccgg tgcgaggtca aaggcacgac
    16441 cggacgggaa gccgtttacc tggtctagcc ccgagacacc caccaaccgc acggttgcgc
    16501 cacttccggc gcctggtacg tcgtccagcg tgaatggccg tgctcccccc gtggttgcgt
    16561 cttcccgttc ttgctcgcga accattcgca gaatggtctc gatgtcatct tgggttagtt
    16621 cggcccgggc aaatatccgg cgcagtgctt cctgctgcca gggtctgagt ccgccggccc
    16681 acccaataat ctcgtccaac aatggcatat ccgtctcctt tccagatccg ccgattaaag
    16741 gccgcgccca gttggatggc aagtggccag cgccttcgag gtgcttcgcc gtgttccgtt
    16801 tgcctgcctt cctgaccgcc accctagtgg cttgtccggt cgtgacgcta cacgggcaga
    16861 atttctgcat gctggccagt atcaagccgc tctaaagtcc gctaccggaa agcgctgttt
    16921 agcggtgaga gacggggaaa gtgcggaagc tactaccgac gcaagcgatg tggtgagcag
    16981 tcctcaggta tgtgcgaaat tgcactcctc ttgaccacca atttgggttg gcgttgacca
    17041 cggattgcgt tcaacttgac cagggcgtgt cactgtcacg accgtcagag aacggccaaa
    17101 aacggacacc gccaacccgt tggaaaaaaa actgagcgtt gctttccgtt aagtttggta
    17161 gttgcaagcc ccgttcagga agaattgctc gatcaacgcc gccatacctg cgcgtgcgat
    17221 acggccgcgg cgctcggcgt ctaccaggcc gaagatcaag gaggagaaga tttcggtaag
    17281 cgccggggca ccgatatcga tacggaatac gcccagtttt tgcccgcgga gaaagaactc
    17341 gtccagcgtt tccgagtaag gcagccatcg gcagccgcct gcatctaaat caaacgtgtc
    17401 cgggcgccac tggaatgaga ggaataccaa caactccctg tgaatcaaat ggccttcgat
    17461 cagacgatgc aatgcgtcca agggcgccgc ctcaagatcg gcatcggcaa tgaccctata
    17521 aataaccacc gaaccatgat tcagaagcat ctcgatcagg ttgtcgcggg tgccgcaaaa
    17581 cctgttgagc ctcgccttgc tgacccccgc tgcctgggct atttctttga acgtggctcg
    17641 cgggtggtca acgatggcga tcgccagggc cttcagcaat ttctcatcgg cagcagttaa
    17701 atccatcggt cactctttat ctatcgcggg cagatgcggg tattttgcct agaacactca
    17761 tttttctcaa agaggatact agctttgttc acaccaaaat caaatgagtc aatattgact
    17821 tatttgattt ttgcgtggat catgcgcggc ttcgactacg tactggcttc gtgcttgggt
    17881 gagatatgaa caaattccgc gaatggatca ctttttccgt gatctcctgt ttggtcgccg
    17941 tgaccctggt tggctgcgac aagcccgaag agcaacggga agaggcgcca gcgcgtgaag
    18001 tcgatgtgct cagcgtgaag accgagccct tcacagtgtt cgccgagcta ccagggcgca
    18061 tcgagccggt gcgtgtcgcc gaggtgcgcg cgcgggtggc ggggatcgtg ctcaagcgca
    18121 ccttcgagga aggggccgac gtgaaggccg gtgacgtgct gttccagatc gaccctgcgc
    18181 cgttcaaggc ggccctgtcg cgggcgcaag gtgaactggc tcgcgccgag gcgcagctgt
    18241 tccaggctca ggcgatggtt cgccgttacg aaccactggt gaagatcgac gccgtcagcc
    18301 agcaggattt cgacaacgcc atggccgctc tgcagagcgc ccaggccgac aagcgctcgg
    18361 cccaggccaa tgtcgaaact gcccgcctgg acctgggtta tgccgaggtt cgcgccccca
    18421 tcgccgggcg tatcggtcgc gcccaggtca ccgaaggggc tctggtaggc cagggcgaag
    18481 ccacgctgct ggcacgtatc cagcaactcg atccggtgta cgccgacttc acccagcctg
    18541 ctgccgatgc cctgcgcctg cgcgcggcaa tcgccgaggg caaggttgcc ggtgcgagcg
    18601 accagccgct gtcgctgcgt gtcgacggta ccgatatcga gcgcaagggc acgctgctgt
    18661 tcaccgatat ctcggtggat cgcagcaccg ggcagatcgc cctgcgaggc cagttcgaca
    18721 accccgaggg cgtgctgctg ccgggtatgt acgtgcgtgt gcgcacgccg caggggctca
    18781 accagaacgc catcctggtg ccgcaacgtg ccgtgcagcg ttcggctgac ggccaggcca
    18841 gcgtgatgct gctgggcgag ggcgataccg tcgaggtgcg ccaggtcact actggcgcca
    18901 tgcaaggctc gcgctggcag atcagcgagg gcctgcaggc cggcgacaag gtgatcacca
    18961 gctcgctggc ggctatccgt ccgggcgcca aggtcatccc acgcgagcaa ggcgccgccg
    19021 aaaaagctcc acagtcccag gcccagtaag ccggagaacc attcatgccc ctgtttttca
    19081 tccgacgccc caattttgcc tgggtagtcg ccctgttcat ctcgctaggt ggcctgctgg
    19141 tcattccgtt cctgccggtg gcacagtacc ccaacgtggc gccgccgcag attaccgtga
    19201 ccgccaccta tcccggcgcc tcggcgcagg tgctgaccga ctcggtgacc agcgtcatcg
    19261 aggaagaact caacggcgcc aagaacctgc tgtacttcga gtccaccagc aacgccaacg
    19321 gcatagccga gatcaccgtc accttccagc cggggaccga ccccgaactg gcccaggtcg
    19381 acgtgcagaa ccgtctgaag aaggccgagg cgcgcatgcc gcaggccgtg ctgaccctcg
    19441 gcatccagac cgagcaggcc accgccggct ttctgctgat ctattcgctg cgctacaagg
    19501 atggcgacaa gaacgccaac accacggcac tggcggacta cgcggtacgt aacgtcaaca
    19561 acgagattcg ccgtctgccc ggtgtcggca agctgcagtt cttcgactcc gaagccgcca
    19621 tgcgcgtgtg gatcgacccg cagaaactgg tcggttacgg cctgtccatc gacgacgtca
    19681 acaacgccat ccgcacccag aacgtgcagg tgccggccgg cgccttcggc agcacgccgg
    19741 gctccagcga gcaggagctg accgcgacgc tgaccgtcaa gggcaccctg gacaaccccg
    19801 aggaattcgc cgccatcgtg ctgcgtgcca accaggacgg ctcgcgcctg accctgggcg
    19861 acgtggcacg catcgaggtc ggcagccagg actacaactt cggctcgcgc caggacggca
    19921 agcccgccgt tgccgccgcc gtgcagctgt cgcccggtgc caacgcgatc cagaccgccg
    19981 aggcggtcaa gcagcgtctg accgagctgt cggccaactt cccggacaac gtcgagttct
    20041 ccgtgccgta cgacacctcg cgcttcgtcg acgtggccat cgacaaggtc atcatgaccc
    20101 tcatcgaggc catggtgctg gtgttcctgg tgatgttcct gttcctgcag aacgtgcgct
    20161 acaccctgat cccgtccatc gtcgtgccgg tgtgtctgct tggtaccctg accttcatgt
    20221 acctgcttgg cttctcggtg aacatgatga ccatgttcgg catggtgctg gccatcggca
    20281 tcttggtgga cgacgccatc gtggtggtgg agaacgtcga gcggatcatg gccgaggaag
    20341 gcctggcgcc ggtgccggcg accatcaagg cgatggggca ggtgtccggg gcgatcatcg
    20401 gtatcaccct ggttctgtcg gcggtgttcc tgccgctggc cttcatggcg ggttcggtgg
    20461 gggtgatcta ccagcagttc tcgctgtcgc tggcggtgtc gattctgttc tcgggctttc
    20521 tcgcgctgac cttcaccccg gcgctgtgcg ccacgctgct caagccgatt cctgtaggcc
    20581 atcacgagaa gactggcttc ttcggctggt tcaaccgcaa gttcaccagc ctgaccagcc
    20641 gctacacgaa gctcaacgac aagctggtgc cgcgagccgg gagggtgatg ttcatctacc
    20701 tgggcgtggt ggtgctgatg ggctttctct acatgcgcct gccggaatcc ttcgtgccgg
    20761 tggaagacca gggttacatg atcgtcgaca tccagctgcc gcccggcgcc acccgtgagc
    20821 gtacctcggc cgctggtgga gagctggagt catttctgat ggcccgcgag gccgtgcaga
    20881 cgaccttcct ggtgcttggc ttcagcttct ccggcatggg cgagaacgcg gccattgcct
    20941 tcccgctgct caaggactgg tccgagcgag attcttcgca gtcgccggaa gccgagtcgg
    21001 ctgcggtcaa ccagcacttc gccaacctcg atgacggcgc gatcatggcg gtgccgccac
    21061 cgccggtcga aggcctgggc aactcgggtg gtttcgccct gcgcctgcag gaccgcgccg
    21121 gccttggccg cgatgccctg ttggcagcgc gcgatgaagt gctgggcaag gtcaacggca
    21181 atccgaagtt cctctacgcc atgatggaag gtctggccga ggcgccgcag ctgcgcctgg
    21241 tgatcgaccg tgagcaggcg cgcacgctgg gagtcagctt cgaagcgatc agcagcgcgc
    21301 tgtccactgc gttcggctcg tcggtgatca acgacttcgc caacgccggc cgccagcagc
    21361 gcgtggtggt acaggccgaa c
//
