LOCUS       NZ_CP113210             2042 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SCLC4-1 plasmid pSCLC4-1_9, complete
            sequence.
ACCESSION   NZ_CP113210
VERSION     NZ_CP113210.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31838583
            Assembly: GCF_026626285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,672
            Genes (coding)                    :: 5,514
            CDSs (with protein)               :: 5,514
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 96 of 158
            Pseudo Genes (internal stop)      :: 13 of 158
            Pseudo Genes (multiple problems)  :: 14 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113210.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCLC4-1"
                     /isolation_source="environment"
                     /db_xref="taxon:573"
                     /plasmid="pSCLC4-1_9"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            complement(join(1463..2042,1..389))
                     /gene="mobV"
                     /locus_tag="OT485_RS28930"
                     /old_locus_tag="OT485_28935"
     CDS             complement(join(1463..2042,1..389))
                     /gene="mobV"
                     /locus_tag="OT485_RS28930"
                     /old_locus_tag="OT485_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_267727937.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTVAMASQSAQNAKRARELQKTMDSQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            1073..1399
                     /locus_tag="OT485_RS28935"
                     /old_locus_tag="OT485_28940"
     CDS             1073..1399
                     /locus_tag="OT485_RS28935"
                     /old_locus_tag="OT485_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001070513.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQIDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(4..437)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.931"
                     /coverage="99.0"
                     /evalue="5.17e-96"
     Typing          complement(1467..2041)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.875"
                     /coverage="99.0"
                     /evalue="1.07e-104"
ORIGIN
        1 acgaacgcag acaggtgcgg ggtggcctcg tccctgtgaa ccaccgccgc cacaacgcga
       61 tctttcccgt atttgttctc cagccattgc tcagaacggg cgaaaaactc cgcctgctgc
      121 cgcggtgtgg cctcgttcca ccactcaggg cttgccgtca taacgtattc aacagccagt
      181 accgcatctt tacggcgttt ttccggcaac agctcccgca gctttcccat cgcctcatct
      241 gccgactgag acacagagca gtagttttct ggtgtccgtt cagcgtcagc attcggcgtc
      301 tcacgctccc tgtagcagtg ctgcaaagcg ctggccacac tgcccattcc cgtcagcttt
      361 ttgcagcgca ttatcgcgta attcgccatg cttaccccct cgttctggcg tacatccagc
      421 cagtcggggg atcgcattgc gatgcctccg gcgggccact atcaggatga tagtgtactc
      481 actagacttt gctacgcaaa atcgttcggg cagcaagctg ccagaaggtc aaaatcaacg
      541 gcagtctggc aggtggaagg cgtcgctccg cgccaccccc caccactgcc aagcctgttt
      601 tatccggtga gtcgtcaagg gtaaatggca ctcgcttcgc tcgcccctga cgaaatcagg
      661 caatgaatat cgtttttgaa agagcaggaa ccgctcgccg caagaacgga ttagggagcg
      721 gcatcgccgc gagtctgtcc gttcgaggaa gcggaatttc cactccccgc acatagaaat
      781 tcggacgctc aatactggca gaaaaacacc tgattttgcc gatgtatgag tacggagagt
      841 aataagatac agaaggggat ctctcggaag ccgcgccaca agcggaaagt ttttgacaat
      901 agttatctct agagataact tagttatctt gagagataac tattttttcc cgaatggaga
      961 tttttagatg cggagaatca cgcaggtaga ccaaagtaca ggggaggaat taggtggttt
     1021 tgtcgcagtg attcgtccta aacaaaagtc atcattccag aggcacttta ctatgaacca
     1081 agccgcgcta attaccattg ccaatgagct aaaccatgac cagatgcgtg tgctgatggc
     1141 cttgctcgca gaactggatt acgaaaacta catccaagtt gcgcagatag atattgcgga
     1201 agcgctgact atgcaaaaaa caaatgtcag tcgtgcggta aaaaacctga ttgatttcgg
     1261 gatcatactg gaaggaccga aaattgggcg gagcaaaacg taccggctaa atccccaatt
     1321 cggctggaaa ggtactgtca gcaaccacaa gaaagcacta aaaaacggcc tcagcgttat
     1381 acagggcggc agaacctgat ctctggaatg ccgcaaatga cctcgttttg cggcgtcaga
     1441 cctatcctgc atcgcatcaa aactaacgac tcataccacc cataccacgc gatcgcgtct
     1501 gtcgctgttt ttcacgttca atacgctgct gtttttcctt ttcctggttt tgctgcttaa
     1561 accgctgagc catcatcagg atttcggtca tctgctctct ggaaaggccc ttaaaaggct
     1621 ctgtaacgct ctgtaggcgt ttctgctgcg aatccatagt cttctgtagc tcgcgcgctc
     1681 tttttgcatt ctgggcgctc tgagaggcca tagcgacggt tcctgcaaag ccatcgttca
     1741 ctgcctggct taaacgtttg gctatggctg cctgatcttc cacgtctttg gtgaaaatac
     1801 ccttccgcag tacacgcggc tccagcgcct ccggtgagat cgatacctgg ctacgggtgc
     1861 cacggtttat cgattcgtag tagtgctgaa ctgtctgatg cgtggcacgg cttccctcga
     1921 tgccgcgctc aagacccagt ttttttaccg attcagcgta ggtgctctga tcgtcacgca
     1981 tctttgaccg cccgccaata aactctttcg cggacagccg cccatcctgc gtcaatggca
     2041 cc
//
