LOCUS       NZ_CP113208             3953 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SCLC4-1 plasmid pSCLC4-1_7, complete
            sequence.
ACCESSION   NZ_CP113208
VERSION     NZ_CP113208.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31838583
            Assembly: GCF_026626285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,672
            Genes (coding)                    :: 5,514
            CDSs (with protein)               :: 5,514
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 96 of 158
            Pseudo Genes (internal stop)      :: 13 of 158
            Pseudo Genes (multiple problems)  :: 14 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113208.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCLC4-1"
                     /isolation_source="environment"
                     /db_xref="taxon:573"
                     /plasmid="pSCLC4-1_7"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            complement(375..530)
                     /locus_tag="OT485_RS28875"
                     /old_locus_tag="OT485_28880"
     CDS             complement(375..530)
                     /locus_tag="OT485_RS28875"
                     /old_locus_tag="OT485_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="WP_064761103.1"
                     /translation="MNDTWDFYTDGKGEWRWRRTASNGRIVGASSQGYVNRADCIANAR
                     RNGYTG"
                     /name="DUF1508 domain-containing protein"
     gene            1006..1773
                     /locus_tag="OT485_RS28880"
                     /old_locus_tag="OT485_28885"
     CDS             1006..1773
                     /locus_tag="OT485_RS28880"
                     /old_locus_tag="OT485_28885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094543585.1"
                     /translation="MRFLWRPLPYSRLRECDFLNCIDENDSSSWIAALKIFICIVIKSD
                     LDENGEYCAMITYPQVQKMTGMSRQLICCGFDKLKFYKMISSEGVRKKKYSLLTCEKGS
                     TRQKPLTKRFDGLSTGRWCKFPLKGLIDDDGVIPSFLAMSNRNINDLHALRLFIYLLSV
                     RRHGDVFIVVKAETLMKKLRLKRVQLTLALAHMSALGLIVKERYREGDFLSLNKTPAYA
                     FLLCGWDALEWNHNRIKESNWRNDYLESFDNWV"
                     /name="hypothetical protein"
     gene            complement(1794..1967)
                     /locus_tag="OT485_RS28885"
                     /old_locus_tag="OT485_28890"
     CDS             complement(1794..1967)
                     /locus_tag="OT485_RS28885"
                     /old_locus_tag="OT485_28890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153848860.1"
                     /translation="MDIKTKLKRSLDSVESAISSLKRARGQTTDAYHEINKAIRELEDA
                     EYNIRKSIREME"
                     /name="hypothetical protein"
     gene            complement(2304..2453)
                     /locus_tag="OT485_RS29000"
     CDS             complement(2304..2453)
                     /locus_tag="OT485_RS29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077253766.1"
                     /translation="MHEPPVQSDCCACSGNYQLESNPERHDKAPLAAATGKQDVREMYL
                     EFLK"
                     /name="replication initiation protein"
     gene            2869..3060
                     /locus_tag="OT485_RS28890"
                     /old_locus_tag="OT485_28895"
     CDS             2869..3060
                     /locus_tag="OT485_RS28890"
                     /old_locus_tag="OT485_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3060..3461
                     /locus_tag="OT485_RS28895"
                     /old_locus_tag="OT485_28900"
     CDS             3060..3461
                     /locus_tag="OT485_RS28895"
                     /old_locus_tag="OT485_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023287146.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIRAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2345..2747)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.545"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa gaacgccgga aacaggactt
       61 accctggctg aatggcgtga attttcgcgc tatgcacttg cgtgcatact catgcatgcc
      121 gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc ctgttttacc
      181 ggtttcccgt cagaaacgcc ctgaggccgt tttagcggtg cgtacaatca tgcctttatg
      241 ttaaatgaca aatgcaaaaa agcccccgaa ggggcctgaa ggttttcctg tatagggagg
      301 aaaaataggt gccctgtttg gcagtacccg ttaccggctg acagccacat tccagtcgta
      361 tacctgattc aggactagcc ggtgtaacca ttgcggcggg cattagcgat gcaatccgct
      421 cggtttacat agccctgact ggatgcacca acaatacgac cattgctggc cgttctgcgc
      481 cagcgccatt cgcctttacc atcagtataa aaatcccatg tatcgttcat tctgttttta
      541 tcctgtgctg tgtggggatc cccaaggttg atataaagca ttcctcctct ttttcaatcg
      601 ataagagtgt gttttgcgat gcgagtacga taggaatttc tgttacatgt tactcatgca
      661 atgccgtctc attaggtcta aaggcttctt gtgatgcaca tagactatct tggtatctta
      721 atagactatc ttttgttgtt ataacattga ttttaaaggt ttttgtattt tgttatattc
      781 tagaaagatt ataataaaaa gacttataag tctttttatt atgtgtcatg attttttcaa
      841 gatttgagca gaggtgtttt cgggaggaga gtatcatgag tctagaaaag tatgtgaagt
      901 tactcgagag agttgcagcg atttctggtg agttaaccca gcttgtaatc tctgtgacgt
      961 tattaataat ttcttggtat cttattaaat aattaaggtg aagctatgcg gtttttatgg
     1021 aggcctttgc cttactctag gttaagggaa tgtgattttt taaactgtat tgatgagaat
     1081 gattcgtctt catggatagc cgcattgaaa atattcattt gcattgttat taaatctgat
     1141 ctggatgaga atggagagta ttgtgcaatg attacgtatc ctcaagtcca gaaaatgaca
     1201 gggatgagta ggcagttaat atgttgtggt tttgataagc tgaagtttta taaaatgata
     1261 tctagcgagg gtgttaggaa aaaaaagtat tcattattaa cttgtgaaaa aggctccact
     1321 agacaaaaac ctttgacgaa aaggtttgat gggctatcca cggggagatg gtgtaagttc
     1381 ccgttaaaag gattgattga tgatgatggt gtaatccctt cttttctggc tatgagcaat
     1441 agaaatatta acgaccttca tgcattgcgg ttgtttatct atttattgtc agtgaggagg
     1501 catggtgatg tttttattgt tgtaaaagca gaaacgctga tgaaaaaatt acgacttaaa
     1561 agagttcaac taacattggc tttagctcat atgtcagctt tggggttgat agttaaagag
     1621 cgatataggg agggcgattt tttgagttta aataaaacgc ctgcatatgc ttttttattg
     1681 tgtggatggg atgctcttga gtggaatcac aatagaataa aagagagtaa ctggagaaat
     1741 gattatttag agtcgtttga taactgggtt tgaagcccag ttatctatat aactcactcc
     1801 atctcacgaa tggatttcct tatgttgtac tcggcatcct caagttcacg aattgctttg
     1861 tttatttcat ggtatgcatc cgtagtttgc cctcttgcac gctttaaaga tgaaattgca
     1921 ctttccacag aatctaaaga acgtttaagc tttgtcttga tatccatatt gaacctctgt
     1981 tttttatctg ctgctagggt gattgtggaa caccctaatg aatttgtttt atttaaataa
     2041 aatcaatatg atacgtcttt ttttgaagtc tattggtatt gttaagtggt gctggtttgg
     2101 ttttgatata tgtctatgta tctaacggat ttgcttgtga tcctttcctc ggcgtcgtag
     2161 tctcttgctc tgtaaacgaa aaaaccaccc ggcaaggtgg tttttcgaag gttcattaac
     2221 cggcaagttt atgaaccgtg gtaacagtct tgtgcgagac atgtcaccaa atccgtcctt
     2281 ccagtgtagc ctcagttagg tcttcacttc aagaactcta aatacatctc tcgcacatcc
     2341 tgtttaccag tggctgctgc cagtggcgct ttgtcgtgtc tttccgggtt ggactcaagt
     2401 tgatagttac cggaacaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
     2461 agcttggagc gaactgcctt cccggaactg agtgtcaggc gtggaatgag ataaacgcgg
     2521 ccataacagc ggaatgacac cggtaaaccg aatggcagga acaggagagc gcacgaggga
     2581 gccatcaggg ggaaacgcct gatatcttta tagtcctgtc gggtttcgcc accactgatt
     2641 tgagcgtcag tttctgtgat gcttgtcagg ggggcggagc ctatggaaaa acggccgcaa
     2701 agcggccttt ccttttcccc gtcacgcgat cgctcggttt tctcgagttt ttacacgcat
     2761 gctcctgccg ctcgccgcag ccgaacggca ggagcatagc gactgagtga gcgaggaagc
     2821 ggaaaagaga acatccctac actgcgcact gacgctctga ggcctcttat gacgaaacaa
     2881 cagaaaacag cgcttaacat ggctaaattc atccagaatc agtctctgct cctgctggag
     2941 aagcttaatg agcttgatct cgatgtcgag gccgaccttt gtgagaaact ccacgatgat
     3001 gcggagcatc ttttccgtac actgtcctcg agactggatg cattacagga tggtaactga
     3061 tggccagagt gtctattagt gaggctgccc gactgacggg taagagtaga acaacactgc
     3121 acaggttaat tagagcaggt gacctgtcca cctgtagcgg tgaacgcaat gcaaaaatgg
     3181 tagatacttc agagcttttg cgtgtctttg gtccctttga gcaaccgaag ggtgaacacc
     3241 attctggaca ggtctctgaa cagcgtgata cagcctctgc tgaccagagt gaacaggtga
     3301 ttcgtcatct gaaacaggag attgaacacc tgaacacctt acttttgtcc aaggattcgc
     3361 acattgacag cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca aaattaactg
     3421 aatccccggc gccatggtgg aaattctgga aaaaatcctg atcctgccat gcttgagttt
     3481 tcctctccgg ccatcccggg gagttcctga accttcccaa aaatcgccag atctctgaac
     3541 gctcctcacg actaaaaata cccacacact acgcaaaatt aaaattttgc agatttgtat
     3601 ttaatatcag ttgtttactt tcctgaaatt tcgtttttta aattttattt acatagatcc
     3661 gactctatga cgactcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc
     3721 aggactataa aaacaataag ttaataacga cccgactcta gacacgactc cccgtttggt
     3781 tgttttttag acgcggcgac tctgggtacg actctagtga agagtcgccg gtgaatcgtg
     3841 tggtgagtcg tccggcgact tgccataacg tgattgccgg ccgtcgacga acccgattcc
     3901 tgaacgtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc cac
//
