LOCUS       NZ_CP113207             6510 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SCLC4-1 plasmid pSCLC4-1_6, complete
            sequence.
ACCESSION   NZ_CP113207
VERSION     NZ_CP113207.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31838583
            Assembly: GCF_026626285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,672
            Genes (coding)                    :: 5,514
            CDSs (with protein)               :: 5,514
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 96 of 158
            Pseudo Genes (internal stop)      :: 13 of 158
            Pseudo Genes (multiple problems)  :: 14 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113207.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCLC4-1"
                     /isolation_source="environment"
                     /db_xref="taxon:573"
                     /plasmid="pSCLC4-1_6"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            366..629
                     /locus_tag="OT485_RS28830"
                     /old_locus_tag="OT485_28835"
     CDS             366..629
                     /locus_tag="OT485_RS28830"
                     /old_locus_tag="OT485_28835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290305.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLARQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYSMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     gene            complement(1360..1623)
                     /locus_tag="OT485_RS28835"
                     /old_locus_tag="OT485_28840"
     CDS             complement(1360..1623)
                     /locus_tag="OT485_RS28835"
                     /old_locus_tag="OT485_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072072086.1"
                     /translation="MAAHTSETVKFLQSVSSDQTWWKSRYNPGDSVPYSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(1693..1821)
                     /locus_tag="OT485_RS28840"
                     /old_locus_tag="OT485_28845"
     CDS             complement(1693..1821)
                     /locus_tag="OT485_RS28840"
                     /old_locus_tag="OT485_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072201952.1"
                     /translation="MSLKPGPKRIADSTGQPDRRQRDNKKTPGNTDKLKPSKSSSK"
                     /name="hypothetical protein"
     gene            complement(1877..2245)
                     /locus_tag="OT485_RS28845"
                     /old_locus_tag="OT485_28850"
     CDS             complement(1877..2245)
                     /locus_tag="OT485_RS28845"
                     /old_locus_tag="OT485_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290306.1"
                     /translation="MKTTSEFARDAQKWDDRELGASENHVEVASMADMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAERKQALRRELDKLNEREEIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            complement(2442..2735)
                     /locus_tag="OT485_RS28850"
                     /old_locus_tag="OT485_28855"
     CDS             complement(2442..2735)
                     /locus_tag="OT485_RS28850"
                     /old_locus_tag="OT485_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290307.1"
                     /translation="MTIVISKEIEEKLTTKHKVTKKEVHESLANLNGNLLIDNREQHKT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNEIERKIYATHGKKTT"
                     /name="hypothetical protein"
     gene            complement(3344..3457)
                     /locus_tag="OT485_RS28995"
     CDS             complement(3344..3457)
                     /locus_tag="OT485_RS28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3868..4059
                     /locus_tag="OT485_RS28855"
                     /old_locus_tag="OT485_28860"
     CDS             3868..4059
                     /locus_tag="OT485_RS28855"
                     /old_locus_tag="OT485_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4059..4460
                     /locus_tag="OT485_RS28860"
                     /old_locus_tag="OT485_28865"
     CDS             4059..4460
                     /locus_tag="OT485_RS28860"
                     /old_locus_tag="OT485_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_267727927.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIRAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4975..6174)
                     /locus_tag="OT485_RS28865"
                     /old_locus_tag="OT485_28870"
     CDS             complement(4975..6174)
                     /locus_tag="OT485_RS28865"
                     /old_locus_tag="OT485_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000948429.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            complement(6184..6372)
                     /locus_tag="OT485_RS28870"
                     /old_locus_tag="OT485_28875"
     CDS             complement(6184..6372)
                     /locus_tag="OT485_RS28870"
                     /old_locus_tag="OT485_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957857.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     Typing          complement(3345..3820)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt
       61 cgacgaaccc gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgctt
      121 cacggccacg caataaattg cgtccccgtt caccgttttc ggatgggaag aacgccggaa
      181 acaggactta ccctggctga atggcgtgaa ttttcgcgct atgcacttgc gtgcatactc
      241 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
      301 tgttttaccg gtttcccgtc agaaacgcct tgaggccgtt tttgcggtgc gtacaagaag
      361 acgttatggt aaatctaaat gatatctgca gaagcaaaaa agccgcctgg ataggcggct
      421 ttttcatttt tttggcgcgg cagaacggca ttttcgacac caactctggt tggttgctcc
      481 tgagggtaca gttcttaagc cgaacagtgc tgagagctgt cctgctgtat ttgcagttgt
      541 tacgttacag catgggcatg tatgaggcag accgaggttt ccagacattc ttcatcactc
      601 cattttttta ccaacaaacg tggatatgac tgcacccaag gcgatcatgc ctaatgtgcc
      661 gactccgccg ccaatacctg atgtacatga cggacacaac tcatcgtcat acaaacgacc
      721 acccttagcg taattctcac accccggagc gcgacatatg taaattgctt ctttgcactc
      781 agggcattcg cacttatctc gtcgtacatg gttttcttga attacacgag gtataacttc
      841 agctttacaa aaaggacata accctttgtg ttttgatgtc attttgacct acttaattgc
      901 actgcaacta attaattttg aacgacattt agggcagtag ttcggtgttc caaacccact
      961 cctactagtc cattcatgcc gacaagccat gcatttagcc cgatagataa caacaaccgc
     1021 catgaaaaac caccattaat atcagtaact aatattcatt tataataact gacaaactaa
     1081 acaagtaaag taaattgtgt atttttttaa gtgattgatt tttaatttat ttttctatta
     1141 tctaaaggct gtaaatttta attaatgcat taaatacaat gacttaatct gatttgtttt
     1201 ttttgtttaa cttttttgga gggcgagaaa gactcatgca tctcgttcgt gcgctcatgc
     1261 agcgtaacga ttgttagcat acaagaagtg cggggctggg atgttatggt aaatatcttc
     1321 gagcttcagg tgcaaaaaaa agccccggag ggctctcaat catttgattc cgaaacggtc
     1381 acctttagtg tccgttctga caatcaattg ccattttatt gcttgttgct gactgtgctg
     1441 atggtggttt tgtggtggga acgggtcgct gccgttactg gtgatctctt ttccgcaaac
     1501 tgtacatcga taaattccag aatagggaac gctgtctcct ggattatatc gtgacttcca
     1561 ccatgtttga tcgctactga cgctttgtaa aaacttcact gtttcgctgg tatgtgcagc
     1621 catttagctt tccttttctg aatttatagg actgacaatg aaaagccgcc taaaaggcgg
     1681 cttttaactg attcatttac ttgaggattt actgggtttt aatttatcag tatttcccgg
     1741 tgttttttta ttgtcacgtt ggcgtctatc aggttgccct gttgaatctg cgattctttt
     1801 tggaccaggt ttaagactca tgataatcct cctttcaaac gcgatgaaat tttttctatc
     1861 tagatggtaa gctctttcat gcttgttttc ttatgctttc aatgaattca tcaactggaa
     1921 ctgtgtcctc ctgctgatgt atttcttctc gttcattgag cttgtccaac tctctacgta
     1981 atgcttgttt tctttctgca ataacaaatc gttgcagaag gtctctaacc attggttgat
     2041 aacctatacc atagctttca gctatcgatt ttaaatcgct tacaagattt ttctgtaatc
     2101 ttattgagat ggcttgcagt gatagtgcat cgttgaaagc tgacatgtca gccatcgatg
     2161 ctacttctac gtgattctca gaggcgccga gctctcgatc atcccatttt tgtgcatcac
     2221 gtgcgaattc actggtagtt ttcattgtat agacctctag gtgctgtttc ctgcagatca
     2281 ggattaactg caatatttat gctttatcta gttccgaaag gaacttctat gttgagaaat
     2341 caggttcagc ttcattttca ttttagttct ttcgctgatg taagattttt gcaaggtctt
     2401 acatggtaag gccttgcaaa acaattactt atttagcgct gttatgtcgt cttttttcca
     2461 tgcgtagcat agattttacg ttctatttcg ttcggttcga aggctgtttt gatatgaatg
     2521 tcgccatcga taatcatgaa acacactttc aactctctac gctggttagt ttcagcgata
     2581 aaccagttgg ttggaggatt ggttttgtgt tgttctctgt tatctatcaa caggttaccg
     2641 ttaaggtttg caaggctttc atgtacttct tttttagtta ctttatgttt tgtggttagt
     2701 ttttcttcaa tttctttact gattacaatt gtcatatttc atgatgcctc ggcctttgtt
     2761 gtgaaattga attcattgta tatacaaaat ttacatttgt caatccatgg cttgattgtt
     2821 aaggtgtttt cattttctat gtgtatatac ataggctgtt tcgattgctt cttcgcagcc
     2881 tttggtccgt tgatattggt tatgaaagat agatatagaa gaagttatga gcaatacaat
     2941 agcattgcct cattcatgtc attgttctga ctatgattat cgattgaacc cagtcgtagc
     3001 tttgacctat caagaggttg acgctctatt tggttttaga aatctacgcg gcaacgtagc
     3061 tcctcagacg tggtgtagag tttgtcgtgt taaaggtgct gcagatagca aaaagaaaaa
     3121 ggcacaatct taggctgagg atcttcttga gatcatgtgt tagtcgtcgt aatctcactg
     3181 ctgtgaaaac gaaaaaaccg ccctgcaagg cggttttttc gaaggttctc tgagctacca
     3241 actctttgaa ccgaggtaac tggcttggag gagcacagtc accaaaactg ttcgtttagt
     3301 ttagccttaa ccggcgcata agttcaagac tatctcctct aaatcagtta ccagtggctg
     3361 ctgccagtgg cgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
     3421 aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     3481 ccttcccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     3541 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac
     3601 gcctggtatc tttaagtcct gtcgggtttc gccaccgctg atttgagcgt ccgtttctgt
     3661 gatgcttgtc aggggggcgg agcctatgga aaaacggcaa cgccgtggcc ttttccacag
     3721 gctcttttct tcctggttat cgcgatgact cttctgtgtg tcccgtccgc tcgccgcagc
     3781 cgaacggcag gagcgtagcg acggagtgag cgaggaagcg gaaaagagaa catccctaca
     3841 ctgagcactg acgccctgag gccccttatg acgaaacaac agaaaactgc cctgaacatg
     3901 gctaaattca tccagaatca gtctctgctc ctgctggaga agcttaacga gcttgatctc
     3961 gatgtcgagg ccgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca
     4021 ctgtcctcga gactggatgc attacaggat ggtaactgat ggccagagtg tctattagtg
     4081 aggctgcccg actgacgggt aagagtagaa caacactgca caggttaatt agagcaggtg
     4141 acctgtccac ctgtagcggt gaacgcaatg caaaaatggt agatacttca gagcttttgc
     4201 gtgtctttgg tccctttgag caaccgaagg gtgaacacca ttctggacag gtctctgaac
     4261 agcgtgatac agcctctgct gaccagagtg aacaggtgat tcgtcatctg aaacaggaga
     4321 ttgaacacct gaacacctta cttttgtcca aggattcgca cattgacagc ctgaaacagg
     4381 ccatgctcct tcttgaaaat caccaggcaa aattaactga atccccgacg ccatggtgga
     4441 aattctggaa aaaatcctga ccccgtcatg cttcagtttt cctctccggc catcccgggg
     4501 agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac
     4561 ccacacccca cgcaaaatta aaattttgca gattccttat ttacaacaat gggttaatga
     4621 aaaataaatg tgcggtgatg tgttacaaag tgcagatcca agtcgtggac aactctatgg
     4681 cgaatcgctg acgaattgcc cacgattcat atgtgaatca cgctttatta tcatattgtt
     4741 atgatgaatc gactcttgat tcaattcatc actctgggtt ttttttgttc atggtgactc
     4801 ttgacgtata ggaaattgaa aaaccagtcg ataccattgt ccacgcctta tgccacgtga
     4861 gcctttctgc ttttcccacc actccggtgg tttccctctc tgtatggtcg atttttatac
     4921 cgtaacaggc tgttttttat actttcaccg attcccgggc acacttagcc tgtgtcaggc
     4981 cggcatatac ctcagtagac tgatatcccg cgcgtttttc ttcagccctg atacattcag
     5041 ccccgcaatg gcccggcggt ataccatccg gcagccacac agcacgcatt cgaacgggtc
     5101 acgactcagg aactgtttca ccatttgtgc atagcacact ttcgccactg gttccggttt
     5161 atccatcccc agtgcacggt acacctgcgg cagcttctct ccacacacac ggttggcaag
     5221 gaacccgaag tacctcacca tcttaaaaaa cttctccggg atgtgctgtt tcagcctcgc
     5281 gaccagctca cgctgtgtca gcgtttccgt cgccgtttct cccgttttgt ggtccaggta
     5341 acggaagctc aggcttgccc ctccgttgta atgagccagt cgggaagccg ctattggtgg
     5401 cttcttcaga taacgaccca ggtagcgtgc cgtattccgc cctccggctg tcttcttcga
     5461 catgtacaca tgccagtatt ttccgccgga tttcagcacc aggcttctcc actgtgattc
     5521 cgtcgtgata tgtgacaacg actccggcat tgccatcccc tctgaccacg ctttcagaag
     5581 cagctgccgc atattccaca tccaccgtga acgcatcgcg tctttcagga agctcagctt
     5641 tttccactga ccatgcttat tcagacctcc acaggttaca gacacatgta catgcggatg
     5701 ccagttgaga cgacggccat acgtgtggat ggcgcagaag ataccgggtt ccagcccccg
     5761 ttttcgggcg gcatacagca gattctccac cgccagacgg cacacgtcat tcagcagcca
     5821 gcggttgctt tcgaacaccg gccacagcgt gtccggcagg gtgaagacca gatgtaccca
     5881 gtcgcagtca ggaagacgat tcagctgtgt tgctgtccac aggtctgtgg ccttctttcc
     5941 gcaggacggg caggcacggc tgccgcatga gttggtcagg tactttacgt gctgacagtc
     6001 cgggttatca cacccgaact cttttacacc cagtatccgt gtgccgcagg ccagcatttt
     6061 ggtgacggct tcaacctcga tatcgcgcag accgcccgca tccaggaagg acgtccagca
     6121 ctggttggct gtgaacagac gtttcagagg gcggggagta aaaccggaca acatgaggat
     6181 ggattaaacc ccggtatcct gtagcagggt aagggctgct gtatcgccac ggcgcttcag
     6241 gatggaggtc gtcaccatcg caggtggtgg gtccatgggt ttgagcattt tcatccagtc
     6301 ccattctcgt tgtgagagct tctcagcacc aacagagagc agaactgtat cgatactttt
     6361 gcgggtcaac ataaggggat gagtccttaa taaaacaggt cctcagagca tacatattct
     6421 gcggggcgtg cggcaacaac aggacgaacc ggggcggttt tcaatttgca gccgccaggc
     6481 tgccgtggtt cattcaattc acgattcgaa
//
