LOCUS       NZ_CP113196             4018 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SXC4-2 plasmid pSXC4-2_5, complete
            sequence.
ACCESSION   NZ_CP113196
VERSION     NZ_CP113196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31836897
            Assembly: GCF_026626185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:28:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 64 of 146
            Pseudo Genes (incomplete)         :: 90 of 146
            Pseudo Genes (internal stop)      :: 14 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113196.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SXC4-2"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pSXC4-2_5"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            280..765
                     /locus_tag="OT483_RS27510"
                     /old_locus_tag="OT483_27510"
     CDS             280..765
                     /locus_tag="OT483_RS27510"
                     /old_locus_tag="OT483_27510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            769..984
                     /locus_tag="OT483_RS27515"
                     /old_locus_tag="OT483_27515"
     CDS             769..984
                     /locus_tag="OT483_RS27515"
                     /old_locus_tag="OT483_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1155..1640
                     /locus_tag="OT483_RS27520"
                     /old_locus_tag="OT483_27520"
     CDS             1155..1640
                     /locus_tag="OT483_RS27520"
                     /old_locus_tag="OT483_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_208484249.1"
                     /translation="MSFTVAAAENNDNVTYTKPSAGRDLYLTCKGYQVKGASVINVEKP
                     SYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMHEHWSLVHVGGRASV
                     KGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIYTRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            1895..2107
                     /locus_tag="OT483_RS27525"
                     /old_locus_tag="OT483_27525"
     CDS             1895..2107
                     /locus_tag="OT483_RS27525"
                     /old_locus_tag="OT483_27525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(2536..2649)
                     /locus_tag="OT483_RS27605"
     CDS             complement(2536..2649)
                     /locus_tag="OT483_RS27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            3297..3620
                     /locus_tag="OT483_RS27530"
                     /old_locus_tag="OT483_27530"
     CDS             3297..3620
                     /locus_tag="OT483_RS27530"
                     /old_locus_tag="OT483_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3007..3184
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.667"
                     /coverage="83.0"
                     /evalue="2.1000000000000002e-48"
     Typing          complement(2537..3057)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.564"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1076
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.944"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3590..4017
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.105"
                     /coverage="94.0"
                     /evalue="2.8e-86"
ORIGIN
        1 ccatagtgaa cggcagactg gggctgcacg acccgaacgc gccggaaaac cggcgggcgc
       61 tggttacacc gtccgcgctg ccggaagcga agcaggaagc cgcccaggcg attacgcgcg
      121 gcctactttc ccttgcctca tccggggagc tgaaaacccg tcaggacgtc actgaggcgc
      181 tggaaagtgc gggttttgag gtggtgcgca ccacgaaaag cagcatcagc attgccgacc
      241 ctgacggggg gcgaaacatc cgactgaagg gagccatcta tgaacagtct tttaacgctg
      301 gcgaaggact tagagcagaa atcgaaagcg cagcagcaga ctaccggcga gatgctgaaa
      361 gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag cgagaagaga
      421 atcagcgccg ccatcctcga ccacgaccgg aagctgtcct cagccatgag ccaacgcacg
      481 aaagggatgc tgcgtatgat cagccagacg tggctgacca tagtcctggt ctccgcgcta
      541 ctgatcgcct cgagcgcggg cattctgtgg tggcaggggc aacagatact cgacaactac
      601 acgaccatcc aggaacagaa gagcacgcag gccatgcttt cagagaagaa caacggcgtc
      661 cagctcacga cctgcgggga ggagagacgc cgctgcgtga gggtgaaccc ggaagcggga
      721 agattcggag aggattcgag ctggatgata ctggcgggga aatagcacat gacggaactg
      781 gaaaaacagt tgctgagcgc attagagcag ctacagcagg actactcgaa aaggctggac
      841 gagtgggaga acgccttcgc ggaatggcgg acgatgtctg ggcttatgca acgggagaac
      901 gcggcgctga gcgagcgcgt cacgggcttg agcaggcagg tgcagagttt gagcgatcag
      961 ctgcgccgat tgtcgcaagg ctgaatggca tcgaggcgca ccgggagcag gagcgcgcgg
     1021 cgcagcacca gaaagcgctg gagctggaac gatcgcagcg gcagaaaggt tttgagcttg
     1081 gaatgtagtt aatcaatgaa aagggatgcg ttatgaaatg tttctgggct tgctcagtct
     1141 cggtggtttt ggctatgtca tttacagtcg ctgctgcgga gaataacgac aacgttacat
     1201 atacaaaacc gtcagccggg agggacttat atcttacctg taaaggttat caggtgaagg
     1261 gggcatctgt aattaacgtc gagaaacctt cttaccttga aggtactggt gagcaggttg
     1321 aatttgcaat cattactcct gttggtgcgt ctgacggact caaggtcttt tttgattcat
     1381 ggggtttttc ttcatttgga ttgattgaaa gccatgtgat gcatgagcac tggtctttag
     1441 ttcatgttgg ggggcgtgct tcagtcaaag gtgaaaagat tgattattca tttgtagata
     1501 accagccaca ggaggggggg agctataaac ccaaaacagg tgtgttcctg tacggctgca
     1561 tgaactcgga taaaagacca tcttgggtcg aaaagatcta cacaagaaca ggtgtgtttc
     1621 gagggggcat agaagactaa aatactgctt acagcatgaa aatccattga acagagtgtc
     1681 agttcatttc cgtgcataac tatgcatacc gtaagaactt acggggggca tttatcggcg
     1741 tttttacgtg gtaagaggtg ctcttttgcg gttcatggcg cagaacggtg ctgaggctgt
     1801 ctgcggcggt gcgaacaagg gatgttatgg taaataagag tgtcttttgg atattttaaa
     1861 tctgtttttt tattaaattt aatgggggca gaaaatgggg gattttgagc gtacctttgg
     1921 agctggtgct gattttgatc ggataattga tgagaataac agcgaatatt ttcgagaaga
     1981 tcgccgaaaa actaaaaatt caaaaaaaaa attctcttct tttcaagatg ctttggtttg
     2041 ggcgaaagct aatcctggca aaacgataat aagatctcca gacggtgtgg ggtttattga
     2101 aaaatgaaag agtggattca ctgtaaaaca acagtcattt tttggacaac actgtctata
     2161 tacgtactca tatgtttcac agtgtcgtat gttggagtct atgttacgta cgttgcaggg
     2221 cctattttgt tagttacagg cattcttgca tggcttacga aacctagctg taatccaaag
     2281 tgactgggct agacgaactt tatcccccca taaaacccgc ttttttaaac ggatcttctt
     2341 gagatcattc atggagaatc gtaatctctt agcactgtaa acgaaaaaac cgcccagcaa
     2401 ggcggttttt tcgaaggttc tcagagcagc aactctttga accgaggtaa ctggcttgga
     2461 ggagcacagt caccaaaact tgtcctttca gtttagcctt aaccggcgca taacttcaag
     2521 actaactcct ctaaatcagt taccagtggc tgctgccagt ggcgttttgt cgtgtctttc
     2581 cgggttggac tcaagtcgat agttaccgga taaggcgcag cggtcggact gaacgggggg
     2641 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     2701 aattagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     2761 gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt cctgtcgggt
     2821 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     2881 ggaaaaacgg caacgccgca tcctttttcc gccctttctg attgatttcc gcttagtctc
     2941 tttatccagc cattcgtaag ctggcaacgc ccgccgcagt ctcacggcag gagcgcagcg
     3001 accgagtgag cgaggaggcg tcatttcacc gggatctgca tttcgcactt acgccccaca
     3061 gcagcatttt ctccctgcca catgaagcac tttcctgaat ccccactccg atttaccatg
     3121 ttaagccagt atacactccg ttagcgctac gtgactggtt cagggctgcg ccccgaaacc
     3181 cgctgaaaag tactgccgcg ccctgcgggc ttgcccaaca ccgctccgga caggaaaaaa
     3241 tttcccgccc gaaccggtgt tatccggagg atggtttggc agaaaagcgc aacaaaatgc
     3301 tcactatgtg ggtgacagaa gacgagcacc gccgacttct ggagcgctgt gagggtaaac
     3361 aactcgcggc ctggatgcgc cagatatgcc tggacgaaaa gccctcgcgt gctggcaaac
     3421 ttccatccat ctcgccggcg ctgcttcgtc agcttgccgg catgggtaac aacctcaacc
     3481 agattgcccg gcaggttaac gccggtggcg gtactgggca cgatcgcgtg caggttgtcg
     3541 ccgtgctgat ggccattgat gccggactcg agcggctgcg gcacgccgtg ctggaaaagg
     3601 gggcagacga tgatcgttaa gtttcatccc agaggccgcg gcggtggcgg cggtccggtg
     3661 gattatctgc tggggaaaga ccggcagcgc gacggcgcca gcgttctgca gggtaagccg
     3721 gaggaagtgc gggagcttat tgacgcctcg ccttacgcca agaaatacac ctccggcgtg
     3781 ctgtcttttg ccgaacagga tttgccgccc ggccagcgtg aaaagctgat ggcgagcttt
     3841 gagcgggttc tgatgcccgg actcgataaa gaccagtaca gcgtactgtg ggttgagcac
     3901 cgggacaaag ggcggctgga gctgaacttc ctgatcccga acacggagct gctgaccggc
     3961 agacgtctcc agccgtatta cgaccgcgcc gaccgtccgc gcattgatgc ctggcaga
//
