LOCUS       NZ_CP113195             4232 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SXC4-2 plasmid pSXC4-2_4, complete
            sequence.
ACCESSION   NZ_CP113195
VERSION     NZ_CP113195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31836897
            Assembly: GCF_026626185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:28:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 64 of 146
            Pseudo Genes (incomplete)         :: 90 of 146
            Pseudo Genes (internal stop)      :: 14 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113195.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SXC4-2"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pSXC4-2_4"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            complement(join(4042..4232,1..97))
                     /locus_tag="OT483_RS27480"
                     /old_locus_tag="OT483_27480"
     CDS             complement(join(4042..4232,1..97))
                     /locus_tag="OT483_RS27480"
                     /old_locus_tag="OT483_27480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074184133.1"
                     /translation="MSDDSSVNLVPVTVFESQVEAVEGITIVVRAPSNTMVPEYTYVRR
                     SPGDNTVGEWMNNRINPSLNGLEVTIIDGEHESPRRSTKRLSTLRDSYER"
                     /name="hypothetical protein"
     gene            complement(925..1326)
                     /locus_tag="OT483_RS27485"
                     /old_locus_tag="OT483_27485"
     CDS             complement(925..1326)
                     /locus_tag="OT483_RS27485"
                     /old_locus_tag="OT483_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1326..1517)
                     /locus_tag="OT483_RS27490"
                     /old_locus_tag="OT483_27490"
     CDS             complement(1326..1517)
                     /locus_tag="OT483_RS27490"
                     /old_locus_tag="OT483_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1932..2045
                     /locus_tag="OT483_RS27495"
                     /old_locus_tag="OT483_27495"
     CDS             1932..2045
                     /locus_tag="OT483_RS27495"
                     /old_locus_tag="OT483_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074184130.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDNAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2248..2973)
                     /locus_tag="OT483_RS27500"
                     /old_locus_tag="OT483_27500"
     CDS             complement(2248..2973)
                     /locus_tag="OT483_RS27500"
                     /old_locus_tag="OT483_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_074184131.1"
                     /translation="MASMETEQQPQKNIEQIEVKAKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKDASIENVFWFSAKPSHLSRTVNDNYQNYTNALTECGVDVIGGRF
                     KRKNVKCEADNGCGEVFHKHEEKESDVNLAISLVSGACNNEYDIAIIITADSDLCPPIK
                     YVKERFPDKELWLVAPPGRIKRAADLCLMSSKNMEINSNALRASCMRDEFYKGGVLVAR
                     RPQEWRAIANTGQRRTRSR"
                     /name="NYN domain-containing protein"
     gene            3292..3903
                     /locus_tag="OT483_RS27505"
                     /old_locus_tag="OT483_27505"
     CDS             3292..3903
                     /locus_tag="OT483_RS27505"
                     /old_locus_tag="OT483_27505"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184132.1"
                     /translation="MSTENAQKEQKKESLITTHWGEDATLHGWTAVPNSLLMLQGDLGI
                     GSTEMCILLNVLMHQWPESGESISFPSIGTIASRMGVSKRTIQRGVSNLESLGILTRHQ
                     STRNDPRTNGANIFDSTPLKEHLNKKSKGITISRNKKKERKNIGTISNIKLPRLCPKCL
                     KTKATSYEEIVSFFGIRKTMAGEVINSYCKECRASEKNIF"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1638..2045
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.195"
                     /coverage="99.0"
                     /evalue="1.29e-162"
ORIGIN
        1 atggtgctct aaccacgatg gtaataccct caaccgcttc aacctgcgac tcaaaaaccg
       61 tcacaggaac taagttaaca ctggaatcat cagacattat attgacctta ccaagcaatt
      121 aaattctagt tagcaggaag ttagcgagta acccaacaaa tgtctactga aaatttcaaa
      181 atgtacgatt taccataacg tcccattacg cgcaccgctc aaacgctcca gaaacgattc
      241 tggggcgtaa cccattaaat cctacgtctt accccctgaa aacgcctcaa aatcgcgctg
      301 atgggtttta cgggcatgaa ttgttatgca ccagactggc tggaggtaga tttcatcctg
      361 aattcacgcc agacaactca aaacccgcac caaacccgac tgaaaacggt gaacggggac
      421 gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac
      481 gttcaggaat cgtgttcgtc gacggccggc aatcacgtta tggcaagtca ccgaacgact
      541 caccacacga ttcaccggcg actcttcact agagtcgtac ccagagtcgc cacgtctaaa
      601 aaacccccaa acggggagtc gtgtctagag tcgggtcgtt attaactcat tgtttttata
      661 gtcctgagtc gttaccgact ctatgacgac tcgccgggga ctctatgacg agtcgtcata
      721 gagtcggatc tatataaatc aaatttaaaa aacgaaattt caccaactta agctactgat
      781 attaaataca aatccgcaaa atgttaattt tgcgtggggt gtgggtattt ttagtggtga
      841 ggagcgttca gagatctgac gatttttggg aaggttcagg aactccccgg gatggccgga
      901 gaggaaaact gaagcatgac ggggtcagga ttttttccag aatttccacc atggcgccgg
      961 ggattcagtt aattttgcct ggtgattttc aagaaggagc atggcctgtt tcaggctgtc
     1021 aatgtgcgaa tccttggaca aaagtaaggt gttcaggtgt tcaatctcct gtttcagatg
     1081 acgaatcacc tgttcactct ggtcagcaga ggctgtatca cgctgttcag agacctgtcc
     1141 tgaatggtgt tcacccttca gttgttcaaa gggaccaaag acacgtaaaa gctctgaagt
     1201 atctaccatt tttgcattgc gttcaccgct acaggtggac aggtcacctg ctttaattaa
     1261 cctgtgcagt gttgttctac tcttacccgt cagtcgggca gcctcactaa tagacactct
     1321 ggccatcagt taccatcctg taatgcatcc agtctcgagg acagtgtacg gaaaagatgc
     1381 tccgcatcgt cgtggagttt ctcacaaagg tcggcctcag catcaagatc aagctcgtta
     1441 agcttctcca gcaggagcag agactgattc tggatgaatt tagccatgtt cagcgctgtt
     1501 ttctgttgtt tcgtcataag gagcctcaga gcgtcagtgc tcaatgtagg gatgttctct
     1561 tttccgcttc ctcgctcact cagtcgctac gctcctgccg ttcggctgcg gcgagcggca
     1621 ggagcatgcg tataaagctc gagaaaaccg aacggtcgcg tgatgggaaa aggaaaggcc
     1681 gcgcagcggc cgtttttcca taggctccgc ccccctgaca agcatcacag aaaacagacg
     1741 ctcaaatcag tggtggcgaa acccgacagg actataaaga tacctatcat ttctgatagg
     1801 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1861 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     1921 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctta tccggtaact
     1981 atcgacttga gtccaacccg gaaagacacg acaacgcgcc actggcagca gccactggta
     2041 actgatttag aggaggtggt cttgaagtca tgcgcctatt aaggctaaac tgaaagaaca
     2101 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagcg ccaactctat
     2161 gaaccttcga aaaaccgccc gccaaggcgg ttttttcgtt tcaacctagc tagattaatc
     2221 tccttactct agctacgtca acaaagccta acgacttcga gttcttcttt gcccggtatt
     2281 ggcaatagct cgccattcct gaggccttct ggctacaagc actccgcctt tgtagaactc
     2341 atcacgcata cagctagctc tcaaagcatt actgttgatc tccatatttt tcgatgacat
     2401 aaggcagaga tctgccgctc gcttgattcg ccccggtgga gctacaagcc aaagttcttt
     2461 atcagggaat cgctctttta cgtatttaat aggagggcat aagtcagaat ccgcagtgat
     2521 aataatggct atatcatact cattattaca agcaccagac actaggctta tagccaaatt
     2581 aacatcggat tctttttctt catgcttatg gaaaacctca ccacatccat tatcagcttc
     2641 gcactttaca ttttttcttt taaaacgccc accgataaca tcaacaccac attcagttaa
     2701 agcattcgtg taattttgat agttatcatt taccgttctc gataagtgtg atggcttggc
     2761 agaaaaccaa aaaacattct ctatcgatgc atctttagga aggtatcttt tacataaaga
     2821 tttaaggtca acccaatgaa gagctttatt acccgtttct tcaatagcat gatataaatt
     2881 atagccatca atgaaacact gaactcgctt tgcttttact tcgatttgct caatattttt
     2941 ctgtggttgc tgctctgttt ccatacttgc catatttgac ctctagatag caaaaaggcc
     3001 tgcatatgca ggcctttttg cgtctcttaa ccgcagttaa cagaccactg tatggacgca
     3061 tccgatacag gaagaattgt gtacaacgcg cttccaagcc tcgacctgga tagccactaa
     3121 gagcagaact cttaggtagt gaactcaaac taatttctat ataaaaaaag agcaaatgga
     3181 tgacatatat gtcatcctat aaagatacat gttactacct aactactctt tataagagta
     3241 gttaggtagt aactgttcac gttttgattt tttttcacga gaaaagcacc catgagtaca
     3301 gaaaacgcac agaaagaaca aaaaaaagag tcattaatca ctacgcactg gggagaggat
     3361 gcaactctac atgggtggac agctgtacca aacagcctcc ttatgctgca aggcgatctt
     3421 gggattggga gtactgagat gtgtattcta ctgaatgtat taatgcatca atggcctgaa
     3481 tcaggggagt caatatcttt tccttcaatt ggtaccattg catcaagaat gggtgtatct
     3541 aaaagaacga ttcaacgtgg agttagcaat ctagaatcat taggaatatt gaccagacac
     3601 caatcaacaa ggaatgaccc tagaaccaat ggggcaaata tattcgattc aactccacta
     3661 aaagaacatt taaataaaaa atctaaaggt ataacaatta gcagaaacaa aaagaaagaa
     3721 agaaagaaca tcggaaccat atccaatata aaattacctc gactttgccc taaatgttta
     3781 aaaactaaag caaccagtta cgaagagatc gttagttttt ttggcatccg aaagacaatg
     3841 gctggggaag taataaattc atattgcaaa gaatgtcgag cttctgaaaa aaacatattt
     3901 taaatattaa aacatataaa tatatgggaa aaggtgcaga gtccaattaa aactttgccc
     3961 catttaccat aacgcctcat tgcccgtacc acataaagaa gccgctgtat aagcggcttc
     4021 tcttattcat ggtgatagcg gttatcgctc gtaactatcc cgtaaagtag ataggcgctt
     4081 agttgaacga cgaggcgatt catgctcccc atcaataatc gtgacttcaa ggccatttaa
     4141 tgacgggtta attctattgt tcatccactc tcctacggtg ttatcgccag gggatcgtct
     4201 aacatacgta tattcaggaa ccatagtatt cg
//
