LOCUS       NZ_CP113194             4991 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SXC4-2 plasmid pSXC4-2_3, complete
            sequence.
ACCESSION   NZ_CP113194
VERSION     NZ_CP113194.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31836897
            Assembly: GCF_026626185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:28:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 64 of 146
            Pseudo Genes (incomplete)         :: 90 of 146
            Pseudo Genes (internal stop)      :: 14 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113194.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SXC4-2"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pSXC4-2_3"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            complement(76..480)
                     /locus_tag="OT483_RS27450"
                     /old_locus_tag="OT483_27450"
     CDS             complement(76..480)
                     /locus_tag="OT483_RS27450"
                     /old_locus_tag="OT483_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(1275..1676)
                     /locus_tag="OT483_RS27455"
                     /old_locus_tag="OT483_27455"
     CDS             complement(1275..1676)
                     /locus_tag="OT483_RS27455"
                     /old_locus_tag="OT483_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1676..1867)
                     /locus_tag="OT483_RS27460"
                     /old_locus_tag="OT483_27460"
     CDS             complement(1676..1867)
                     /locus_tag="OT483_RS27460"
                     /old_locus_tag="OT483_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2283..2449
                     /locus_tag="OT483_RS27600"
                     /pseudo=""
     CDS             2283..2449
                     /locus_tag="OT483_RS27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2779..3837
                     /locus_tag="OT483_RS27465"
                     /old_locus_tag="OT483_27465"
     CDS             2779..3837
                     /locus_tag="OT483_RS27465"
                     /old_locus_tag="OT483_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(3867..4124)
                     /locus_tag="OT483_RS27470"
                     /old_locus_tag="OT483_27470"
     CDS             complement(3867..4124)
                     /locus_tag="OT483_RS27470"
                     /old_locus_tag="OT483_27470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            4542..4973
                     /locus_tag="OT483_RS27475"
                     /old_locus_tag="OT483_27475"
     CDS             4542..4973
                     /locus_tag="OT483_RS27475"
                     /old_locus_tag="OT483_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     Typing          1989..2391
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="99.0"
                     /evalue="1.66e-166"
ORIGIN
        1 ctacctactt cacagacgaa tttaccataa catccgtttt tcgaaccata atggcaggct
       61 atagcctgcc gtaattcacc ggaaaaaatc atccacagag tcgctccact catgcccgga
      121 aagagaccac accttgttta tagggggagg ataaccactc ctgcagtacc cgttttttcc
      181 cacgaaatcg aatgccccgt gcgggtatcc attgatcagg agtacgcact tctgactgtc
      241 ttctgaccag ccaattttaa catcagaagg aatgtgaccg tctgaaacgt cttcggcatt
      301 gtagatgtgt actgcgtcca taatcgggtt tccctcgacg gactcatcga gcgcatagaa
      361 atacccggtc cggccatcgt cttcgaacac ggcggccagc atgccctcag gagcgaggct
      421 ctttataacc tgttcctccc cgacaacgag ctgggcctgt atcgtcacta aaatggtcat
      481 caggtctgtt ctcctttcct tggttgcttc ggcagccagc tttcagacaa gcggataatt
      541 taccataaca tccgttatca gcatcgtcag aaacgcgctg agggcgtttc tgacgggaaa
      601 ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt
      661 atggcatgca tgagtatgca cgcaagtgca tagcgcgaat attcacgcca ttcagacagg
      721 gtaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac gcaatttatt
      781 gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac gttcaggaat
      841 cgggttcgtc gacggccggc aatcacgtta tggcaagtcg ccggacgact caccacacga
      901 ttcaccggcg actctgcact agagtcgtac ctagagtcgc cgcgactaaa aaacaaccaa
      961 acggggagtc gggtctagag tcgggtcgtt attaactcat tgtttttata gccctgagtc
     1021 gttaccgact ctatgacgac tcgccgggga ctctatgacg agtcgtcata gagtcggatc
     1081 tatataaata aaatttaaaa aacgaaatct caggaaagta aactactgat attaaataca
     1141 aatctgcaaa attttaattt tgcgtggggt gtgggtattt ttagtggtga ggagcgttca
     1201 gagatctgac tatttttggg aaggttcagg aactccccgg gatggccgga gaggaaaact
     1261 gaagcatgac agggtcagga ttttttccag aatttccacc atggcgccgg ggattcagtt
     1321 aattttgcct ggtgattttc aagaaggagc atggcctgtt tcaggctgtc aatgtgcgaa
     1381 tctttggaca aaagtaaagt gttcaggtgt tcaatctcct gtttcagatg acgaatcacc
     1441 tgttcacact ggtcaacaga ggctgtatcg cgctgttcag agacctgtcc agaatggtgt
     1501 tcacccttca gttgttcaaa gggaccaaag acacgtaaaa gctctgaagt atctaccatt
     1561 tttgcattgc gctcaccgct acaggtggac aggtcacctg ctttaattaa cctgtgcagt
     1621 gttgttctac tcttacccgt cagtcgggct gcctcactaa tagacactct ggccatcagt
     1681 taccatcctg taatgcatcc agtctcgagg acagtgtacg gaaaagatgc tccgcatcat
     1741 cgtggagttt ctcacaaagg tcggcctcga catcaagatc aagctcgtta agcttctcca
     1801 gcaggagcag agattgatgc tggatgaatt tagccatgtt cagcgctgtt ttctgttgtt
     1861 tcgtcataag aggcctcaga gcgtcagtgc tcagtgtagg gatgttctct tttccgcttc
     1921 ctcgctcact cagtcgctac gctcctgccg ttcggctgcg gcgagcggca ggagcatgcg
     1981 tatgaaagct cgagaaaacc gagcgatcac gtaatgtaga aaagggaagg ccgcacagcg
     2041 gccgtttttc cataggctcc gcccccctga caagcatcac agaaacggac gctcaaatca
     2101 gtggtggcga aacccgacag gactataaag atatcaggcg tttccccctg atggctccct
     2161 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     2221 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     2281 gtatgcacga accccccgtt cagtccgact gctgcgcctg ttccggtaac tttcgacttg
     2341 agtccaaccc ggaaggcacg acaaaacgcc actggcagca gccactggta aggggtgtac
     2401 aagaattact ggtgcagagt tcttgaagtt acagcctaac tacagctaaa ctgaaaggac
     2461 agatttggtg gcagcggtct tgtacaccct gttaccacgg ttcagaagtt ccccaactta
     2521 ctgatccttc gaaaaaccac ctccccaggt ggttttttcg tttacagagc aaaagattac
     2581 atcccgcagg ttaggatctc aagaagatcc ttttaatcgg aattcgacaa ctccagataa
     2641 gtcatggtga gccagccaac cccttcgact gaatatcgtt ccaaaaaaag gcagtcagct
     2701 gaaagaaact aacagtagaa caataatctc tagtaccctg aatctaagct aacaacaaaa
     2761 ctagattagg agttatagat ggcgatctat gccgtatgga ataacaaggg tggtgtaggg
     2821 aaaagttacc ttacttttca gttagcctct gaatatgcca gacagaaccc tactaaaaag
     2881 gtcctggtgg ttgacctgtg cccacaagcg aactcgtcct ctatgttact tggtggaatg
     2941 ataaatggcg aaaacacact aaaccagatt cattcagcaa tacagagaaa gacaatatct
     3001 ggatatattg aggataggat cagaagtcca tatattccta ccaacactgg ttctagctat
     3061 gtaataaatg tatctacaca caatagtaaa gttccaaata atctatatct tgtcactggc
     3121 gatgaacaat tagaactcca agcatcaaga gttgttgggg catcatttcc aggtccccat
     3181 gatgcatggc gtactgttca cctctggatt aatgatttaa tcagtgacat ttgcaactca
     3241 tggaataatg aagatagctg cgtattcatt gactgcaacc ctagcttctc tatttataca
     3301 gagttagctt taaccgcagc agagcgcctt ttaattcctt tctctgccga tggttcatca
     3361 aagagagcag ttaaggctgt actttcttta gtttatggtg agcgtcgcca tcagaatgaa
     3421 ccacaatcag atttctatct taatagcaat cgttattcta tgtcgctacc acaaatatat
     3481 atgtatgttg gaaatagatt aacgcaaatg aacaattcat ctgccagtgc atttaagacg
     3541 gtagtaaccg aaattggtga tgaaatttgg aaagtatggc aaacaaaacc aagtctgttt
     3601 tgtattcacc caaatgggag ctcaacgcca aataataaaa gatctttcag gaatatgttt
     3661 cagtatgaag taaatgatgc aaacacagca tctgtcgtat ctggagcctt aggtattcca
     3721 atcgcgacat taactgcagg aaataaaact gtttcaggaa aaataataaa ggttaatcag
     3781 acgcagctag ataaacaagt ccccaacatt gtagctctcg ctggtatgat cgaataaaat
     3841 agcaaggccc ttaaaagggc ctttttttat ttgtttttct ttactgcttg cagcgccctg
     3901 ccagcactag aggccaactc tttgattaca ttatctttac tttcaacaac ctcagtaagc
     3961 aactcgtttt cgttttccag tttattcaaa gcgttctgag tatcttcaat agcatcatca
     4021 tacccttcgg catagccatc catccaacca agagtatacg tttggtccaa aagatcagct
     4081 tgcttatcct gcagatcttt attctgatta ttgttagagc ccatgttttt tcctcatttg
     4141 ttggcgcatc tgagttttca aaccaggagg aattccattt ctaagtttct cacttagtct
     4201 gactggtggt gtgtttcctg agactggatt acagtcagca tccaccccga catctttctt
     4261 tttatagtcg ccaatattcc taacgttatc tgtagtcata gaaaaccacg cccattgctg
     4321 tgaataaccg aactataaac cagaaaaaaa acgtacggct tatcataaca cttgacacaa
     4381 aaaaacaccg ctactctcca agcaaaaaac ataaaattgg aaaaacaaaa tcacaagcac
     4441 atgataacaa tagatattaa tatttactta catttagtga gttttataat tagaaaaaca
     4501 atgtaattta ataatcacaa acattaccta aggacgaact gatgaaaata cccgcgcatt
     4561 tgaagcataa accaattatt caagtcgaaa attacgaccg cattgacggc ccacatgccg
     4621 atgacactga tgctatgggg ctatctgtag gtattgctca atggaacact cctggatgga
     4681 ctgagctttc ggcaaaggtt tggcgaaaca caggagaaaa atggtcaaga caatcagaag
     4741 agctgcctct tcaccgggta atcgaccttg caactctgat ctgtatcacc atggactaca
     4801 gtaaaaatgg gcgactttca tccagtgatg attttcccgt gactcgctca actgataacc
     4861 ctgagctagc acatcacact gagtttatga aaaaagagtt aaaacaaaat aaaagcaatc
     4921 ttgatgtaag cctcaaacgc ctctcgactg aattgaagag acttggctat taaaactagc
     4981 tttagaaatg t
//
