LOCUS       NZ_CP113190             9582 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SYCC2 plasmid pSYCC2_6, complete
            sequence.
ACCESSION   NZ_CP113190
VERSION     NZ_CP113190.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31836844
            Assembly: GCF_026626105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,772
            CDSs (total)                      :: 5,648
            Genes (coding)                    :: 5,480
            CDSs (with protein)               :: 5,480
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 68 of 168
            Pseudo Genes (incomplete)         :: 96 of 168
            Pseudo Genes (internal stop)      :: 30 of 168
            Pseudo Genes (multiple problems)  :: 22 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113190.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SYCC2"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pSYCC2_6"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yanyun Gao"
     gene            complement(join(9137..9582,1..662))
                     /locus_tag="OT482_RS02910"
                     /old_locus_tag="OT482_02915"
                     /pseudo=""
     CDS             complement(join(9137..9582,1..662))
                     /locus_tag="OT482_RS02910"
                     /old_locus_tag="OT482_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(<736..1121)
                     /locus_tag="OT482_RS02915"
                     /old_locus_tag="OT482_02920"
                     /pseudo=""
     CDS             complement(<736..1121)
                     /locus_tag="OT482_RS02915"
                     /old_locus_tag="OT482_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(1359..2279)
                     /locus_tag="OT482_RS02920"
                     /old_locus_tag="OT482_02925"
     CDS             complement(1359..2279)
                     /locus_tag="OT482_RS02920"
                     /old_locus_tag="OT482_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002912403.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_225368972.1"
                     /translation="MMLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDAT
                     HDIITKFTSDNPSIKIKYINSINQGAAKARSTGLNYAEGDYVFFCDSDDMISANFISVF
                     TELSNDKPDMIYFCSTVLDSQGNRFKKVHFPLAYIESNSNEVFNYLLKKGQWTAAVWTY
                     IFRKDLVLKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGSLTKSPKK
                     IDYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQGIMHTAIY
                     RVLWKEKLVILDFVKSKVKKKNHEI"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(2276..3742)
                     /locus_tag="OT482_RS02925"
                     /old_locus_tag="OT482_02930"
     CDS             complement(2276..3742)
                     /locus_tag="OT482_RS02925"
                     /old_locus_tag="OT482_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="WP_167876629.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGMIFCLGLFIFSAQLAS
                     LFNGGTELAKQIKIMSFSLIISSFGGQPRALLARGLRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQLVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     ASLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     gene            complement(4023..4850)
                     /locus_tag="OT482_RS02930"
                     /old_locus_tag="OT482_02935"
     CDS             complement(4023..4850)
                     /locus_tag="OT482_RS02930"
                     /old_locus_tag="OT482_02935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_074177990.1"
                     /translation="MISVNLTTMHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSD
                     HGIYEIPAWVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGRQ
                     WLEFLLDQFYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMKKDKFEKDFIITGVGG
                     CVLSKRMIKPSIIGDDAYLTISPKTDDIWISKMLEASNTKVESSPDALRCVHEILHGYN
                     TLSSINTLTSKNSSLIIKAIRMVKNKFYSYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family A protein"
     gene            5032..5730
                     /locus_tag="OT482_RS02935"
                     /old_locus_tag="OT482_02940"
                     /pseudo=""
     CDS             5032..5730
                     /locus_tag="OT482_RS02935"
                     /old_locus_tag="OT482_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(6105..6218)
                     /locus_tag="OT482_RS02940"
                     /old_locus_tag="OT482_02945"
     CDS             complement(6105..6218)
                     /locus_tag="OT482_RS02940"
                     /old_locus_tag="OT482_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            6790..6939
                     /locus_tag="OT482_RS02945"
                     /old_locus_tag="OT482_02950"
     CDS             6790..6939
                     /locus_tag="OT482_RS02945"
                     /old_locus_tag="OT482_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062850.1"
                     /translation="MPSEHDEQWDAADTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            7420..7611
                     /locus_tag="OT482_RS02950"
                     /old_locus_tag="OT482_02955"
     CDS             7420..7611
                     /locus_tag="OT482_RS02950"
                     /old_locus_tag="OT482_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7611..8033
                     /locus_tag="OT482_RS02955"
                     /old_locus_tag="OT482_02960"
     CDS             7611..8033
                     /locus_tag="OT482_RS02955"
                     /old_locus_tag="OT482_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_015062851.1"
                     /translation="MARVSISEAAQLVKVSRPTIYKMINSGKLSYTSVVKHGKSIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDSESTGINSVVLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPYSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          6583..6797
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.364"
                     /coverage="100.0"
                     /evalue="3.53e-41"
     Typing          complement(6110..6658)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.948"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tcaggcactt gatcctgcga ccggtggcca gcgcgtccat gacaaaatcc atcggccagg
       61 tcaggttggg cgcctccggg cggagcagcg gcagacgttc tgttgccaga cctttacgac
      121 gtcgtctgcg ttttacaccc agcccgttaa gatggtaaag acggtacacg cgcttgtgat
      181 taacatgaag gccttcacgg cgcagtaact gccagatgcg tcggtagcca aaacgcctgc
      241 gctccagtgc cagctcagtg atgtgccctg ataaatgcgc atcagccgcg ggacgctgag
      301 cctcatagcg gcaggtcgac agggacaaac ctgtaagcct gcaggcacga cgttgcgaca
      361 gaccggtcgc atcacacata aactcaacgg cttccgtttc tggtctgtcg tcagtacttt
      421 cgccccagag ccacctgaag tgcctcctta tccagcatgg cttcggcaag cagtttcttg
      481 agtctggcgt tctcttcctc aagcgactta aggcgcttaa cctcgggcac ctccataccg
      541 ccatacatct tacgccaggt gtaaaagatg gcgtcggaaa tggcgtgctt acgggcagaa
      601 accccggctt cggcctcgcg gagaatactg atgatctgtt cgtcggaaaa acgcttcttc
      661 atgaggatgt cctcatgtgg cttatgaaga cattactaac atcacggtgt attaaccaac
      721 ggggagcagg tcactgtcat acgcgtaaaa cagccagcgc tgacgtgatt tagcaccgac
      781 gtagccccac tgttcgtcca tttccgtgca gacgatgacg tcactgccag gatgtatgcg
      841 cgaggttacc gactgcggac tgagtttttt aagtgacgta aaatcgtgtt gaggccagcg
      901 cccataatgc gggcgctggc ccggcatcca acgccattca tggccatatc aatgattttc
      961 tggtgcgtac cgggctgaga ggcggtgtaa gtgaactgta gctgccatgt tttacggcag
     1021 tgagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac caccccgtca
     1081 gtagctgaac aggagggaca gctgatagaa acagaagcca cgcaagcacc ttaaaaacac
     1141 catcatacac taaatcagta agttggcagc atcacccaga aagaccataa aattagccta
     1201 taacggcaaa tagtaatgct gccaacctgt tggtgaccat ttgaatctgt agccattcaa
     1261 tccttgtaac atcaggtgat cctggcgtca gtgaaaatgc gaaatcaaca aattggcatc
     1321 attacccatt atacgataat ccctattggg ataggctctt agatctcgtg attttttttt
     1381 ttaactttgc ttttaacaaa atctaaaatc actaattttt ccttccacaa tactcgatat
     1441 atagctgtat gcattatccc ctgaaacacc atttttttat tttcttttaa tatttgcagt
     1501 agcgaagaca tagaccatcg atcataaaga acaatgcttt cctgactgaa tttgtaaatg
     1561 atatcttccc tggcctcttt ataagactta taacgctcag ttaaataatc aattttttta
     1621 ggggatttag taagtgaacc tgaagtcaca ttctgaaagt aaagctcaga attacatatt
     1681 gatatttttt cactaccagc gagaagtctc agcgtgaata agtgatcttc atgtaccttt
     1741 ctgtctgtaa atatagcgcc agatttcaat acgagatctt tccggaaaat ataagtccat
     1801 acagcagctg tccactgccc tttcttcaaa agataattaa aaacttcgtt actattgctt
     1861 tcgatgtatg ctaatggaaa atgtaccttt ttaaacctgt ttccttgcga atctaatacc
     1921 gtagagcaaa aatatatcat atccggttta tcattactta actctgtaaa aaccgatatg
     1981 aaattagcag aaatcatatc atctgaatca cagaagaata cataatctcc ctctgcgtaa
     2041 tttaagcccg ttgatctggc ttttgccgcc ccctgattaa tactattaat gtattttatt
     2101 tttatagagg ggttgtcact tgtaaattta gttattatgt catgcgttgc gtcccatgag
     2161 ccatcattaa tcaacactag ttgtacggtt tcattaagct gctcttttat tgagagaagc
     2221 aatttttcga tatttgactc acagttataa catggtatga ttattgataa taacatcatt
     2281 tattcctcag tatattgttt agaacgattg cgttaaattc ctttatctct tttactggag
     2341 aggtgagtat agaagcaatg tatacagaca tacatgaaaa aataatcaca accattgaca
     2401 aaataggaga ggtaatagtt aaaaaaagaa tcaacaggta acccgttaca agcataggta
     2461 aagtgtgtat aagcggtacg aagacggcat agatgtaact ctttactttg atatttaaac
     2521 agggtctaac ctggaaaaaa tatcccataa caagatttac tatttgaata cataagaatg
     2581 aaaacactat ccctgtaaga ccatattgtt tacccagaaa ccatgttaaa ggaataccaa
     2641 gcgctaactt aaatactccc aggtaaacat ttctgtacat ttgcccttta gcaagcaaga
     2701 cagagataac aggcacatca agggatcgcg ccgccccaac caaacataaa atctgtacaa
     2761 taggggtgat gaactgccat ttctgctcaa aaaaaacagt cactacatag tctgaaatta
     2821 acatcattcc caataacatc gggaaattca ccaaagacga aaacatagtt acttttagaa
     2881 aagttttttt cagagatgct gcatcatttt gtatcttgga aaaagctgga aataacgcat
     2941 gtgataaaac aggattcaat ttttcaaaaa gcgccacgct aatgttgtag gatatattat
     3001 atccaccgat agcagtcaaa cttattaagc gtgccattag tacaggatat gtattcacgc
     3061 ttatctgatt aatgatagag tccatgacca actgagaacc gtagctgaca tgttttttta
     3121 tttccttcaa agtaaagaac tgtaacttaa tatctctcaa gcctttgctt gaatatatat
     3181 acaatacgct actaatagat gaggaaacca acagtgaaac ggcaacacac cagggggaac
     3241 gatagacgta gcttaagaag actgcgaaca caaaattaaa aatagctgac aggattgtta
     3301 tcctggatat tacatcaaat ctaagtcctc tggctaacaa agctcttggc tgtccaccga
     3361 aagacgatat tataagagaa aatgacatga tttttatctg ttttgccagt tctgttccac
     3421 cattgaataa ggatgctaac tgagcactaa aaataaacaa gccgagacaa aatatcatac
     3481 ctaaaaagac atttattata tacagtgaag acagttcata tttatttaac ttttcagatc
     3541 ttataactga gtttgatata ccaaaatcag ttatgattga gaaaacagta cttaatatta
     3601 atgccgcact aatgatacca taatctgtcg ttgtaaataa gctggacaag atccacagtg
     3661 agaccatacc aagcccggtt acgcacactg tagacaacaa attccacttc acagcatgta
     3721 ttgttttcga ttttaaattc atatttattg tcagtgatat aaaataatta cattggtgta
     3781 ctaaaaggaa ttttttatac ctttcagcgt tagagcctat cccaaagtaa tgctgccaac
     3841 ctgttggtga ccatttgaat ctgtagccat tcaacccttg taatatcagg tgatcctggc
     3901 gtcagtgaaa atgcgaaatc aataaattgg catcattacc caaaaagcaa taacaatttg
     3961 ctaaccacta cagtttactg ataacatttt gttttcagac aatccctgat gagaaagatc
     4021 atttataatt taccattaaa atgcaaatta accatcttga tggatttgtc attgtttgtt
     4081 ttttgcaacc caaaatatga atagaatttg tttttaacca tcctgattgc ctttataatt
     4141 aaactgctat ttttagatgt taatgtattt attgagctaa gtgtgttata gccatgcaat
     4201 atttcatgaa cacatcgcag tgcatctggg gatgactcga ccttcgtatt agaagcctct
     4261 aacattttac taatccagat atcatctgtc ttaggtgaaa tagttaaata tgcatcatct
     4321 ccgataatgc taggcttaat cattctctta cttaaaacac aacccccaac gccagttatt
     4381 ataaaatcct tttcaaattt atcctttttc atactaatag aataagagct ataagatttg
     4441 tacttgttaa aaatattcct ctccattatt ctaatcctag cagcaattgc cacattacag
     4501 ttgttctcat aaaactggtc taataggaac tcaagccact gccgaccata tattacatca
     4561 tcatcagcat atacaagaat gtcatcaatc tctgagttaa taattgcgtt aattatttta
     4621 cggtatgggc cggtattttt tacataatgt acattcacta tgttatttat tttatttagc
     4681 tcttcaaccc aagcaggtat ctcatatatc ccatgatctg acaaatatgg ctcatttgat
     4741 atccaaaggt ttattttatc tggctgaaca ctctggttta taattgacca cactgtggcg
     4801 gcacataaat ccagtcgaga atgcattgta gttaaattaa ctgatatcat gctacccttt
     4861 aaagaaaaaa ttaatttaat gttatattat atgtaaaaag gttacatatt caatatgtag
     4921 gacgtttttt cgaaaaaatc agtttttttc ctgatgaacc ttcctccgcg ttgcccggta
     4981 atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc
     5041 atttccatca gatgcccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc
     5101 actgccggac atcagcgcta tctctgctct cactgccata aaacatggca gctacagttc
     5161 acttacaccg cctctcagcc cggtacgcac cagaaaatca ttgatatggc catgaatggc
     5221 gttggatgcc gggcaaccgc ccgcattatg ggcgctggcc tcaacacgat tttacgtcac
     5281 ttaaaaaact cagtccgcag tcggtaacct cgcgcataca tccgggcagt gacgtcatcg
     5341 tctgcacgga aatggacgaa cagtggggct acgtcggtgc taaatcacgt cagcgctggc
     5401 tgttttacgc gtatgacagg atacggagga cggttgtggc gcatgtcttc ggtgaacgca
     5461 ctctggccac actggagcgt cttctgagcc tgctgtcacc ctttgacgtg gtgatatgga
     5521 tgacggatgg ctggccgctg tatgaatcac gcctgaaggg aaagctgcca cgttatcagc
     5581 aagcgttata ctcagcgcat tgagcgacat aacctgaatc tgagacaaca tctggcaagg
     5641 ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa ggtcatcggg
     5701 cattatctga gcataaaaca ctatcagtaa gttggagtca ttaccgggat atcgttcacc
     5761 acgggaatac ctgcggcggc gaaccagtaa tgggttaagt gataaaaagt gtatggaaat
     5821 ataggggcca atccagctct tcagtcaatg atggttttga tgagcctgaa cattgatatc
     5881 gtcgtagatc ttgaaaggat cttcttgaga tccgtttttg aacgtcgtaa tctcttgctc
     5941 tgtaaacgaa aaaaccgcct ttgcggggcg gtttttccga aggttcgtag agtttgcggc
     6001 tctttgaacc aaggtaactg gctagttaga ggagcgcagt caccaaaact gttcgtttag
     6061 tttagcctta accggcgcat aacttcaaga ctatctcctc taaatcagtt accagtggct
     6121 gctgccagtg gcgcttttgc atgtctttcc gggttggact caagacgata gttaccggat
     6181 aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     6241 gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat
     6301 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa
     6361 cgtctggtat ctttaagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
     6421 tgatgcttgt caggggggcg gagcctatgg aaaaacggct tcgccgcgac cttatcgctt
     6481 ccctgttaag cttctccctg gcatctacta ccaggaagtt tccgtcccgc ccgtaagccg
     6541 gtaccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaat
     6601 atcttctgta tcgcatattc tgcttacgct ctgctgcagc atttttctcc tgccacatga
     6661 ggcgctttcc tgattatcgc actcatgcca acataataag ccattataca ctctgctagc
     6721 gctacgtgac tggttcaggg ttgcgccccg aaacccgcta acaacctctc agctactgga
     6781 tatcatccca tgccatctga acatgatgaa caatgggatg ctgctgatac gcagcttccc
     6841 ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct gcatgcccct
     6901 gtggattccg ggccacctgt cggccgggaa gcgttatagc tttttcgctt gcccgcgtac
     6961 gtggtaatac gccggtatta cattttattt ctttattttc atgtgattac aggtgattgt
     7021 aatacgttgc gtattacata aataagctgg aatttcagtt tttacctttt aattcatgca
     7081 gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat ttaaggggca aaaaattatc
     7141 attctggccg ggtgtactgc gcaggttaag gagcatcggt gacgaaaaag cgtccttagc
     7201 gacctcgttc atgctcgcat gaacggatta tttttccgga ctacaggtaa actacagaaa
     7261 gactacaaaa gctgatgact taatcctgat ttttagtgcg atgactacag ataaactaca
     7321 aaataactac aggattacct cagattcgtt ctctgaacca ttcgtacatc cgaccccctt
     7381 accggtttta cactgagcac tgatgctctg aggtccctta tgacgaaaca acagaaaaca
     7441 gcgctgaaca tggctaaatt catccagaat cagtctctgc tcctgctgga gaagcttaac
     7501 gagcttgatc tcgatgtcga ggccgacctt tgtgagaaac tccacgatga tgcggagcat
     7561 cttttccgta ctctgtcctc tagactggat gcattacagg atggtaactg atggccagag
     7621 tgtctattag tgaagcggca cagcttgtta aggttagtcg ccccacgatt tacaagatga
     7681 ttaacagtgg gaagttaagt tacacctctg ttgtaaagca cgggaagtct attaaagtta
     7741 ttgatacttc agagcttatt agggtgtttg gttctcttga tggtgtaatc gatactgtaa
     7801 aatatgatgt aaaaagtgac tcagaatcta caggtattaa cagcgttgtt ttacatgatt
     7861 tacaacatag aatcgcttta cttgaagctg aaaacgatgg tcttaaaggg gctgttaagg
     7921 ctcgagatga gcatattgat tcccttcgtc aggctatgca gcttctggaa cacaagcacg
     7981 aaccttcttc accaccctat tccccgtggt ggaaattctg gaaaaaatcc tgatcccgcc
     8041 atgcttcagt tttcctctcc ggtcatcccg gggcgttcct gaaccttccc agataccgcc
     8101 agatctctga acgctcctca ccactaaaaa tacccatacc cacaccccac gcaaaattaa
     8161 aattttgaag ataaatcatt aatatcagtt agttagttgt tattaaatta cgcagtatta
     8221 ccttctgtaa ggtcaatcca agtcgtcggc gattcgtgta cgacttgccg acgactcgtc
     8281 atagagtcat cgacgattca ctccacacta tcagtaactt atgacgactt gactctagag
     8341 tcgaatcgtc attttggtgt ttttttgacc ttgacgactc tagagtcaag tcacgagtcg
     8401 aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgccggcc
     8461 gtcgacgaac ccgattcctg aacgtgctga aaatcggttt tccggctgcg ccggcaacgc
     8521 ttcatggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga agaacgccgg
     8581 aaacaggact taccccggct gaatggcgtg aatttccgcg ctatgcactt gcgtgcatac
     8641 tcatgcatgc cgtaaaaaca gagccagcgc gtttctggcg gattttcggg gggggtgttg
     8701 cctgttttac cggtttcctg ccagaaacgc cctgaggccg ttttcgcggt gcgcgtaaga
     8761 agggattatg gtaaataggg cgctatcgcc taaatacacg gctgattgtt ttttttctct
     8821 gaagtatgag attcctgagt agacagctac aacaagtagc gcaatggcga tagcaagtga
     8881 aatccagtcc ataaatgcct gattaattta ataaaaacag cctgttatga tttcataaag
     8941 ataatatcat ctttttttta gcatgatact gtcgttactt attgtggtga gatgaaacat
     9001 aaccgctgtt tttgtatgtg tttgttaagg ctgctaagat tattcctaac cactgatttt
     9061 gtacatatca tcaaaaaaat aaaaaaacta atgccctgtg gtgggtatga cctgccccca
     9121 ggattagata caaccttcag ttagtaatgt cggttcgttt ttcttcatat ttcccgtttc
     9181 gccagcccgc cgcaaattca gccggagtct ggtagttcag cgatgaatgt ggacgacact
     9241 cgctctaatc ctgccgccag tcattaatcg ttttcctcgc gtgaacgata tcgcggaacc
     9301 agtgctcatt gaggcactca tccctgaatc gtccgttaaa actttcaata aatccgttct
     9361 gtgttggcct gcccggctgg ataagccgca gctccacccc atgctcataa gcccactggt
     9421 cgagtgctct gcaggtaaac tccgccccct ggtcagttct tatcgtcgcc ggatagccgc
     9481 gaaacagtgc aatgctgtcc agcattcgcg tgacctgaac gcctgaaatc ccaaaggcgg
     9541 cggtaattgt cagacactcc ttcgtgaagt cgtccacgca gg
//
