LOCUS       NZ_CP113184             3559 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SYCC1 plasmid pSYCC1_7, complete
            sequence.
ACCESSION   NZ_CP113184
VERSION     NZ_CP113184.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31836736
            Assembly: GCF_026626085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:30:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,631
            CDSs (total)                      :: 5,507
            Genes (coding)                    :: 5,353
            CDSs (with protein)               :: 5,353
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 61 of 154
            Pseudo Genes (incomplete)         :: 94 of 154
            Pseudo Genes (internal stop)      :: 26 of 154
            Pseudo Genes (multiple problems)  :: 22 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113184.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SYCC1"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pSYCC1_7"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yayun Gao"
     gene            813..1037
                     /locus_tag="OT490_RS28090"
                     /old_locus_tag="OT490_28095"
     CDS             813..1037
                     /locus_tag="OT490_RS28090"
                     /old_locus_tag="OT490_28095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            1039..1683
                     /locus_tag="OT490_RS28095"
                     /old_locus_tag="OT490_28100"
     CDS             1039..1683
                     /locus_tag="OT490_RS28095"
                     /old_locus_tag="OT490_28100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            1683..2159
                     /locus_tag="OT490_RS28100"
                     /old_locus_tag="OT490_28105"
     CDS             1683..2159
                     /locus_tag="OT490_RS28100"
                     /old_locus_tag="OT490_28105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2366..2479)
                     /locus_tag="OT490_RS28105"
                     /old_locus_tag="OT490_28110"
     CDS             complement(2366..2479)
                     /locus_tag="OT490_RS28105"
                     /old_locus_tag="OT490_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            2895..3086
                     /locus_tag="OT490_RS28110"
                     /old_locus_tag="OT490_28115"
     CDS             2895..3086
                     /locus_tag="OT490_RS28110"
                     /old_locus_tag="OT490_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3086..3508
                     /locus_tag="OT490_RS28115"
                     /old_locus_tag="OT490_28120"
     CDS             3086..3508
                     /locus_tag="OT490_RS28115"
                     /old_locus_tag="OT490_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2367..2774)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 cttcccaaaa atcgccagat ctctgaacgc tcctcaccac taaaaatatc cacaccccac
       61 gcaaaattaa aattttgcag atttgtattt tatatcagta gtttacgttc tataaatttc
      121 gtttttttaa ttttatttat acagatccga ctctatgacg actcgtcata gagtccccgg
      181 cgagtcgtca tagagtcggt aacgactcag gactataaaa acaataagtt aacaacgacc
      241 cgactctaga cccgactccc cgtttggttg ttttttagac gtgacgactc tgggtacgac
      301 tctagtgaag agtcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg
      361 attgccggcc gtcgacgaac ccgattcctg aacgtgctga aaaccggttt tccggctgcg
      421 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga
      481 agaacgccgg aaacaggact taccctggct gaatggcgtg aattttcgcg ctatgcactt
      541 gcgtgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
      601 tggtttgttg cctattttac cggtttcccg tcagaaacgc cctgaggccg ttttagcggt
      661 gcgtacaatt ttggtgttat gttaaatcat ggccacaaaa ttccataaag tgaccaatgt
      721 aatgttaaat gagcattgtg gatgaaagat gttgttttat ggtattattt tttatgttct
      781 aaacttaaat ttatttctat cggggtatta taatgagcaa taaagattct ttagaaagaa
      841 aagtaacacc aagaaaaaat gaacctgtat tcccgatggc tctaagagtt gggatagctg
      901 aagatatttg ttttttagag ttagtggact ttcctgaaaa agaatcagga agggtttttt
      961 attccatagc tctaaccaaa aaacatgcaa agaaccttat cgatggattg aataaattcg
     1021 ttgagtcagg tgattagtat gaaatacatt aaccactgcg atagtgaaga ttattatatt
     1081 actaatgata gcattgatct cggtgttcgt tatgatgttt ctgattataa taagttagag
     1141 ctaaatgatt taagagatcc ggatttgttt aaagttttgg gtcgagcatt tgttgttgtt
     1201 gagaattctg tttgggagtc ttggaatgat gatgttaaaa cattagattg ctctggtgac
     1261 ggttatgagt caattttaaa acattacgaa tcattagaat tatcaggtga attcttattg
     1321 ttttttaaaa aaataagaag tgccatttct gtttataata gagaatacga gttctgcact
     1381 ggtgacctag aaccatcttt gcaggctatt gttcagttga ttaggtatgc ccctgaactt
     1441 gctgcaaaaa aagacgctca gatttacttt gataaaaaat cagagtgttt tgggcttata
     1501 tataaacaaa ctaagaaaag caaaggcacg ctaaatttaa tagtgaaaac aaataacgag
     1561 attatttttt cctttgttag aaaagtttct ggagtggtta aggttacagg tagggcatat
     1621 attggcgata gccttgataa ctccattgct atcaaatcat tatttaaaat gatggagtgg
     1681 tgatgactgt gttatcaact ttaaataacc tcaaatcaat cccttgtatt aatgcattta
     1741 cgggtagagc aaataattca tatttatggc gttatgttat ggaagatcat aaagatgagt
     1801 ttggcgctac aactattaag tgtttttatc ttagggagga aatttcagag gattatgtgt
     1861 ctatttatca ttcaaataaa gattattatg aaggaatggt aagggatgta cataaaagaa
     1921 tccctgtagg gaaaaccaaa cctgccatat atctctcatt tgatgcagcg gaagtgatcg
     1981 agcaagttaa caaaccagat aaatgtattg atataaagga cgaaggttac cctcacttgg
     2041 gtttgtggta tttatttgat attaatgatg aatttacttt gattgaggtg actacaacta
     2101 ttttggaatt atcctctttt tttcaaagaa atgaaggtgg cgtggttgtt cctctttagc
     2161 gaaaaccgtc ttgacgtcat caagtgtgac ctagtactct tcttgaaaaa gaaaaaccgc
     2221 ctttgcgggg cggtttttct taggttctaa gagtttgcgg ctctatgaac caaggtaact
     2281 ggctagttag aggagcgcag taaccaaaat cgttctttca gtttagcctt aactggcaca
     2341 taacttcaag actaactcct ctaaatcagt taccagtggc tgctgccagt ggcgcttcgt
     2401 cgtgtctttc cgggttggac tcaagttgat agttaccgga aaaggcgcag cagtcggact
     2461 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     2521 tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     2581 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttatagt
     2641 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     2701 cggagcctat ggaaaaacgg ccgctgtgcg gcctctcctt ctccccatca cgcgatcgct
     2761 cggttttctc gagcttttat acgcatgctc ctgccgctcg ccgcagccga acggcaggag
     2821 cgtagcgact gagtgagcga ggaagcggaa aagagaacat ccctacactg agcactgacg
     2881 ctctgaggcc tcttatgacg aaacaacaga aaacagcgct gaacatggct aaattcatcc
     2941 agaatcagtc tctgctcctg ctggagaagc ttaacgagct tgatcttgat gtagaggccg
     3001 acctttgtga gaaactccac gatgatgcgg agcatctttt ccgtacactg tcttcgggac
     3061 tggatgaatt acaggatgga aactgatggc cagagtgtct attagtgaag cggctcggct
     3121 tgttaaggtt agtcgcccca caatttacaa gatgattaac agtgggaaac taagttacac
     3181 ctctgttgta aagcatggga aaaatactaa agttgttgat acatcagagc ttattagggt
     3241 ttttggctcg ctttatggtg ggggcgacac tgtaaaacat gatgtaaaag gtgacgcagg
     3301 cgctacaggg gttaacatct ctggtttaca tgatttacag cataaaattg ctttgcttga
     3361 agctgaaaac gatggtctta aaggggctgt taaagctcgt gatgaacata tcgattctct
     3421 tcgccaggct atgcagcttc tggaacacaa gctcgaaccc tctccacagg ttcattctcc
     3481 gtggtggaaa ttctggaaga aatcctgacc ccgtcatgct tcagttttcc tctccggcca
     3541 ttccggggag ttcctgaac
//
