LOCUS       NZ_CP113183             3674 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SYCC1 plasmid pSYCC1_6, complete
            sequence.
ACCESSION   NZ_CP113183
VERSION     NZ_CP113183.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31836736
            Assembly: GCF_026626085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:30:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,631
            CDSs (total)                      :: 5,507
            Genes (coding)                    :: 5,353
            CDSs (with protein)               :: 5,353
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 61 of 154
            Pseudo Genes (incomplete)         :: 94 of 154
            Pseudo Genes (internal stop)      :: 26 of 154
            Pseudo Genes (multiple problems)  :: 22 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113183.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SYCC1"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pSYCC1_6"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-05"
                     /collected_by="Yayun Gao"
     gene            complement(755..1057)
                     /locus_tag="OT490_RS28060"
                     /old_locus_tag="OT490_28065"
     CDS             complement(755..1057)
                     /locus_tag="OT490_RS28060"
                     /old_locus_tag="OT490_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_023317863.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMNDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     gene            complement(1121..1414)
                     /locus_tag="OT490_RS28065"
                     /old_locus_tag="OT490_28070"
     CDS             complement(1121..1414)
                     /locus_tag="OT490_RS28065"
                     /old_locus_tag="OT490_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            1752..2033
                     /locus_tag="OT490_RS28070"
                     /old_locus_tag="OT490_28075"
     CDS             1752..2033
                     /locus_tag="OT490_RS28070"
                     /old_locus_tag="OT490_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2491..2637)
                     /locus_tag="OT490_RS28075"
                     /old_locus_tag="OT490_28080"
     CDS             complement(2491..2637)
                     /locus_tag="OT490_RS28075"
                     /old_locus_tag="OT490_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     gene            3053..3244
                     /locus_tag="OT490_RS28080"
                     /old_locus_tag="OT490_28085"
     CDS             3053..3244
                     /locus_tag="OT490_RS28080"
                     /old_locus_tag="OT490_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023317861.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3244..3645
                     /locus_tag="OT490_RS28085"
                     /old_locus_tag="OT490_28090"
     CDS             3244..3645
                     /locus_tag="OT490_RS28085"
                     /old_locus_tag="OT490_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023317862.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKEGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQGEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2529..2932)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.309"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcattccggg gagttcctga accttcccaa aaatcgccag atctctgagc actcctcacc
       61 acgaaaaata cccacacccc acgcaaaatt aaaattttgc agatagacct ttagtttcaa
      121 ttagttatct attgtaaatt acgcaatttt attttttgta aggtgaatcc gagtcgtggg
      181 cgactctatg gcgaatcgct gacgaattgc ccacgattca tatgtgaatc acgctttatt
      241 atcatatggt tatgatgaat tgactcttga ttcaattcat cactttgggt gttttttgtt
      301 catggtgact cttgattcaa ttcacgagtc gaatcgccgg tgaatcgtgt ggtgagtcgt
      361 ccggcgactt gccataacgt gattgccggc cgtcgacgaa cccgattcct gaacgtgctg
      421 aaaacgggtt ttccggctgc gccggcaacg gctcacgacc acgcaataaa ttgcgtcccc
      481 gttcaccgtt ttcggatgga aagaacgccg gaaacaggac ttaccctggc tgaatggcgt
      541 gaattttcgc gctatgcact tgcgtgcata ctcatgcatg ccgtaaaaac agagcctgcg
      601 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
      661 ccatgaggcc gttttagcgg tgcgtacaat tatgacttta tgttaaatca ttagggaggg
      721 gattctgctg gaacttagtg gatgttattg catatcagac aaatttcggc agatttccca
      781 agttatcgag tttagttttg tttggatctg tggttatgta gccatcgttc atcgcttcca
      841 ctttggctcc taggttccac ttaccagtag agctgttctt tacaactgtc ataaagctgg
      901 cttttcccat gctgatcagc tcgctgatga atttgcgaga gctgattgag ccggcagtgt
      961 tccatgagcc atctcggttt attttatgaa cttttacgaa ttcgatgtgc tgaccagcac
     1021 tgtccatttt gattgctgag atatagaaat ctaccatggt tgcctcctga tggctgctgg
     1081 gtgttttacg taacaaaagc ccgcgttctg cgggcttttt tcatggaaac attggatgca
     1141 agtgttccag ctctgccgct aaagatttgc gactttttga caggctgaat tgggtctgca
     1201 tgtttaccca cgactctgca gagcggccta cgaccttcga caatctaagt gccatttcat
     1261 agcttaagtc tgttttccca tttaacagtc tacttaaggt agactgggat acatcaagtt
     1321 tatcggctaa ctctgcggca ggtatttcga acggttccat gaacatttct ctgataaact
     1381 caccaggatg aacaggatcg taaagattta tcatgccttc ctctgtttgt tttgtatttg
     1441 attatacaaa aaaaattact gcctttcagt atgcgcattg cacaatgtgc aagttcatga
     1501 tattgacaaa aaaagcagtc agctttagga tactcgttgg agtcaaagct actgcgtatg
     1561 tatgggcttt tatggccttt tcaaggcgca gtattttacc cttaggattc gttgaagtca
     1621 aatgaggtga ttgacctcaa gaacaaactt acgtcaattg ttttaataaa gcaagggggc
     1681 aaaatggaac taattaaaat gattttaaaa tatagataca ggtatagacg aaaaaaatat
     1741 ggggattgta gatgataaaa aacataaaac ataaaggact taggagcttt gctgaagaag
     1801 gtagcaccgc cggtattcag cccagtatgg cgaaaagact tcgcatgatt ttgactacgc
     1861 taagcacagc aacttgcatt caagacgttg ttgacgttag ggcgttaggg tgccaccccc
     1921 taaaggggga gcgtaaaggc gagtattccg ttgaagttaa tgggaactgg cgggtagttt
     1981 tcaaggttga ggaaccaaac gtcttagacg ttgatcttga agattatcat tagttatcaa
     2041 taagtaagtt gctaatccat ccaagtgaaa gtacggatgg attagggact ttattaaaaa
     2101 agtaacctct ggagaaagta tgagatcctt tatggtttta ttgttcccgt tgattagcgt
     2161 aactattgct ggagtcctag cttttcgtgg tattgatggc tggggatggt tcttgttagc
     2221 tgctgtactt tgtgcagggg ctgatactgt ccagaagcat gtgtctacca ataaaaagga
     2281 gcctgctggt gagtgagctg tcttggttct tccttgactg ctttgaatga taaagcgtat
     2341 cttactcgcc gagacgaaaa aaccgcctgg ggaggcggtt ttttcgaggg ttcagtaagt
     2401 tggggaactt ctgaaccgtg gtaacagggt gtacaagacc gctgaaacca aattctgtcc
     2461 tttcagttta gccgtagttg ggctgagact tcaagaactc tgtatcagta atctcttgta
     2521 caccttctta ccagtggctg ctgccagtgg cgcttgtgcg tgtcttaccg ggttggactc
     2581 aagatgatag ttaccggata aggtgcagca gtcggactga acggggggtt cgtgcataca
     2641 gtccagcttg gagcgaactg cctacccgga actgagtgtc tggcgtggaa tgagataaac
     2701 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     2761 gggagccatc agggggaaac tcctgatatc tttatagtcc tgtcgggttt cgccaccact
     2821 gatttgagcg tccgtttctg tgatgcttgt caggggggcg gagcctatgg aaaaacggcc
     2881 gctttgcggc ctttcctttt ccccgctacg cgaccgctcg gttttctcga gcttttacac
     2941 gcatgctcct gccgctcgcc gcagccgaac ggcaggagca tagcgactga gtgagcgagg
     3001 aagcggaaaa gagaacatcc ctacactgag cactgacgct ctgaggcctc ttatgacgaa
     3061 acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc tgctcctgct
     3121 ggagaagctt aacgagcttg atctcgatgt cgaggccgac ctttgtgaga aactccacga
     3181 tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgcattac aggatggtta
     3241 ctgatggcca gagtgtctat tagtgaggct gcccgactga cgggtaagag tagaacaaca
     3301 ctgcacaggt taattaaaga aggtgacctg tccacctgta gcggtgagcg taatgcaaaa
     3361 atggtagata cttcagagct tttacgtgtc tttggtccct ttgagcaacc gaagggtgaa
     3421 caccattctg gacaggtctc tgaacagcgt gatacagcct ctgctgacca gggtgaacag
     3481 gtgattcgtc atctgaaaca ggagattgaa cacctgaata ccttactttt gtccaaggat
     3541 tcgcacattg acagcctgaa acaggccatg ctccttcttg aaaatcacca ggcaaaatta
     3601 actgaatccc cggcgccatg gtggaaattc tggaaaaaat cctgaccgcg tcatgcttca
     3661 gttttcctct ccgg
//
