LOCUS       NZ_CP113172             4167 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain TSNTC34-1 plasmid pTSNTC34-1-5,
            complete sequence.
ACCESSION   NZ_CP113172
VERSION     NZ_CP113172.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31832001
            Assembly: GCF_026626065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:18:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,553
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,314
            CDSs (with protein)               :: 5,314
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 49 of 116
            Pseudo Genes (incomplete)         :: 64 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 15 of 116
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="TSNTC34-1"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pTSNTC34-1-5"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-07"
                     /collected_by="YY"
     gene            complement(join(4056..4167,1..1004))
                     /locus_tag="OT488_RS27695"
                     /old_locus_tag="OT488_27695"
     CDS             complement(join(4056..4167,1..1004))
                     /locus_tag="OT488_RS27695"
                     /old_locus_tag="OT488_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(1349..1462)
                     /locus_tag="OT488_RS27700"
                     /old_locus_tag="OT488_27700"
                     /pseudo=""
     CDS             complement(1349..1462)
                     /locus_tag="OT488_RS27700"
                     /old_locus_tag="OT488_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1878..2069
                     /locus_tag="OT488_RS27705"
                     /old_locus_tag="OT488_27705"
     CDS             1878..2069
                     /locus_tag="OT488_RS27705"
                     /old_locus_tag="OT488_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2069..2470
                     /locus_tag="OT488_RS27710"
                     /old_locus_tag="OT488_27710"
     CDS             2069..2470
                     /locus_tag="OT488_RS27710"
                     /old_locus_tag="OT488_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2816..3058)
                     /locus_tag="OT488_RS27715"
                     /old_locus_tag="OT488_27715"
     CDS             complement(2816..3058)
                     /locus_tag="OT488_RS27715"
                     /old_locus_tag="OT488_27715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3424..4056)
                     /locus_tag="OT488_RS27720"
                     /old_locus_tag="OT488_27720"
     CDS             complement(3424..4056)
                     /locus_tag="OT488_RS27720"
                     /old_locus_tag="OT488_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          complement(1350..1757)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tcttcatttt tgctatctat ttcgtttatt gctcgtatca catcttgact atgagttgtt
       61 gcaaatattt gattgttgtt ttgttttgca gatgaaatta atgattttat tatgtcttta
      121 attactgagt aatgtattcc gttctcgatt tcatcaataa gaataattga gttttcctgt
      181 accagcactg agctgataaa agataatgct cgactgatac cctcgccaag cattgatatt
      241 tcatttagct cactaaatcc aatgtctaaa tatatttcct tattgttacc tatcgcagat
      301 attgctatat ctacaattct gtcatctatt ttttttaagt tttctagtat gtcttttttc
      361 cttttttgtg ttagtaggct agatacattt gtaattgttg cctctttgtt tatctttctg
      421 gatgttgtta ttattgttgc tgtcttaaat ttataattaa tcccatccct ttcgtttttt
      481 atttcggatt ttatttctac gccatcttct ttcaattgca tagagaaaag cttattggaa
      541 accctgtttt tcgcattggg tgtctcggtc acatttattt ttaaagagtt tactgtggcc
      601 tgagaaccga tagtttgacg aggtatgttt tggttagata gtacggtgtc aacgtttaga
      661 gacaaacttg attctaatct ctgatttgtt tctgtttcat atgtttgtgt tacttcagag
      721 cgttcatctc gaatgactat ggacatcttt tgtgatgtgt ccatatcatg aaagtaacta
      781 acccaaaatg gcttgttttt gtttatttct gccatttctt ttctgaatgc ttgaatgttt
      841 aacaaaacag ccgggttttt tacatcataa aaacaaaaaa tagcatccag tatagatgtt
      901 ttcccgtagt tgtttttccc agctatgata tttaaagtct ttaattgctt tagatatagc
      961 tcattgaagc atttgaagtt ctttaaactt atttccttta tcatgatagc ctctgttcgt
     1021 gtttttgtta tatgccatac gatttgttag taccttaata aaatgtacag ctttcattgt
     1081 gattagctag actttatgtc aaagattaac tttgattgct tgtgttttaa tcgatttttt
     1141 ttactttctt cttgagatta tctggcagca gccgtaagct tccggctgtg aacgaaaaaa
     1201 accgccttgg cgggcggttt tttcgaaagg ttcagtagag ttggcgctct atgaaccgag
     1261 gtaactggct taaagggagc gcagtcacca aaactgttcg tttagtttag ctttaactgg
     1321 cgtataactt caagactaac tccctaaatc agttaccagt ggctgctgcc agtggcgctt
     1381 tgtcgtgtct ttccgggttg gactcaagac gatagttacc ggattaggcg cagcagtcgg
     1441 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     1501 gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1561 aaggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg gtatctttat
     1621 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttccgtgatg ctcgtcaggg
     1681 gggcggagcc tatggaaaaa cggccgctgt gcggcctttc cttctttcct ccatgcgatt
     1741 actcggtttt ctcgagcgtt tctacgcatg ctcctgccgc tcgccgcagc cgaacggcag
     1801 gagcgtagcg actgagtgag cgaggaagcg gaaaagagga gatccctaca ctgaccactg
     1861 acgctctgag gcctcttatg acgaaacaac agaaaacagc gctgaacatg gctaaattca
     1921 tccagaatca gtctctgctc ctgctggaga agcttaacga gcttgatctc gatgtcgagg
     1981 ccgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca ctgtcctcga
     2041 gactggatgc attacaggat ggtaactgat ggccagagtg tctattagtg aggctgcccg
     2101 actgacgggt aagagtagaa caacactgca caggttaatt aaagcaggtg acctgtccac
     2161 ctgtagcggt gagcgcaatg caaaaatggt agatacttca gagcttttac gtgtctttgg
     2221 cccctttgag caaccgaagg gtgaacacca ttctggacag gtctctggac agcgtgatac
     2281 agcctctgct gaccagagtg aacaggtgat ccgtcatctg aaacaggaga ttgaacacct
     2341 gaacacctta cttttgtcca aggattcgca cattgacagc ctgaaacagg ccatgctcct
     2401 tcttgaaaat caccaggcaa aattaactga atccccggcg ccatggtgga aattctggaa
     2461 aaaatcctga ccccgtcatg cttcagtttt cctctccggc catcccaggg agttcctgaa
     2521 ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac ccacacccca
     2581 cgcaaaatta aaattttgca gattccttat ttacaacaat gggttaatga aaaataaatg
     2641 tgcggtgatg tgttacaaag tgcagatcca agtcgtggac gactctatgg cgaatcgctg
     2701 acgaattgcc cacgattcat atgtgaatca cgctttatta tcatattgtt atgatgaatc
     2761 gactcttgat tcaattcatc actttgggtg ttttttgctc atggtgactc ttgattcaat
     2821 tcacgattcg aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg
     2881 attgccggcc gtcgacgaac ccgattcctg aaggtgctga aaaccggttt tccggctgcg
     2941 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggaggaga
     3001 agaacgccgg aaacaggact tcccctggct gaatggtgtg aatttccgcg ctatgcactt
     3061 gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
     3121 tggtttgttg cctgttttac cggtttcccg tcagaaacgt cctgagtccg ttttagcggt
     3181 gcgtacaatt atgattttat gttaaattgt tttaagtgaa tagagagaat ataacttaag
     3241 ggagcatctt gtgctgggtc ttttacatta gatctgcaca atcattgttt tggcatctgg
     3301 tgatgtcttt aggacgtttg agattgctga taactaaagt gtatgatgct ttctatacgt
     3361 tatccggcgt ttaataacac cctggtgata agccagcccg tgaagggctg gcttgatttg
     3421 gatttatatt tttgaaataa aatcttttat ttcatcaaag caattgtctg ctgtgtttat
     3481 tattccattt ttcatggata ttcctattct tgagtcggat tctctattgc agctaagata
     3541 gacttgtacc ttagcttttt tttcgtttgt tggataagtg tactctttgt cactcgctga
     3601 gatgtcatct ttattatttt taattttttc gaaaaattct attatgtatt catataacgc
     3661 tcgagtgctt gcgtgttcaa ttatcagatc ttcaatggca ccttgcttgt cttttcctgg
     3721 cattataaag aaagaaattt ttgtattgcc attcatagct atctcgttat gcgagctggg
     3781 ggaggagatt tgtagttctc gcaatgtggc aattattgat ctttctgtgt cagaaaatga
     3841 tgtgtcagca tctaatataa taaaaatact ttctttttca tcgaaagaat caatgttttt
     3901 gaatgctcta agtgcttgtt ttaattttga ttttccattt attttttcga caaaaacatc
     3961 ggtaatgcct tgtatttcaa gtaggttgca tagaaaatga aaatcatcat caccctcaac
     4021 gaaaatgatc tttttgctat tatttatgtc tttcattatc ttacctccca gtcgttctca
     4081 actgagtagg agaaatcatc catattgaat tgtacggctg ttggttttag ggagttcttt
     4141 tctcttccta gtctaatata agcgata
//
