LOCUS       NZ_CP113171             4471 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Klebsiella pneumoniae strain TSNTC34-1 plasmid pTSNTC34-1-4,
            complete sequence.
ACCESSION   NZ_CP113171
VERSION     NZ_CP113171.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31832001
            Assembly: GCF_026626065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Emergence and spread of tmexCD-toprJ in live poultry market in
            Yangzhou, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Yin,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) College of Veterinary Medicine, Yangzhou
            univeristy, Shuangqiao, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v. v3.12.0; unicycler v. v. v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:18:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,553
            CDSs (total)                      :: 5,430
            Genes (coding)                    :: 5,314
            CDSs (with protein)               :: 5,314
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 49 of 116
            Pseudo Genes (incomplete)         :: 64 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 15 of 116
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113171.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="TSNTC34-1"
                     /isolation_source="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pTSNTC34-1-4"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-07"
                     /collected_by="YY"
     gene            join(4322..4471,1..108)
                     /locus_tag="OT488_RS27670"
                     /old_locus_tag="OT488_27670"
     CDS             join(4322..4471,1..108)
                     /locus_tag="OT488_RS27670"
                     /old_locus_tag="OT488_27670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            complement(138..1196)
                     /locus_tag="OT488_RS27675"
                     /old_locus_tag="OT488_27675"
     CDS             complement(138..1196)
                     /locus_tag="OT488_RS27675"
                     /old_locus_tag="OT488_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_087834901.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(1526..1692)
                     /locus_tag="OT488_RS27790"
                     /pseudo=""
     CDS             complement(1526..1692)
                     /locus_tag="OT488_RS27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2108..2299
                     /locus_tag="OT488_RS27680"
                     /old_locus_tag="OT488_27680"
     CDS             2108..2299
                     /locus_tag="OT488_RS27680"
                     /old_locus_tag="OT488_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2299..2700
                     /locus_tag="OT488_RS27685"
                     /old_locus_tag="OT488_27685"
     CDS             2299..2700
                     /locus_tag="OT488_RS27685"
                     /old_locus_tag="OT488_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087834902.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMIDTSE
                     LLRVFGPFEQPRGEHHSGQVSEQRDTAFADQSEQVIHHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3474..3905)
                     /locus_tag="OT488_RS27690"
                     /old_locus_tag="OT488_27690"
     CDS             complement(3474..3905)
                     /locus_tag="OT488_RS27690"
                     /old_locus_tag="OT488_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     Typing          complement(1586..1962)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.251"
                     /coverage="88.0"
                     /evalue="1.05e-168"
ORIGIN
        1 aacgaaaacg agttgcttac tgaggttgtt gaaagtaaag ataatgtaat caaagagttg
       61 gcctccagtg ctggcagggc gctgcaagca gtaaagaaaa acaaataaaa aaaggccctt
      121 ttaagggcct tgctatttta ttcgatcata ccagcgagag ctacaatgtt ggggacttgt
      181 ttatctagct gcgtctgatt aacctttatt atttttcctg aaacagtttt atttcctgca
      241 gttaatgtcg cgattggaat acctaaggct ccagatacga cagatgctgt gtttgcatca
      301 tttacttcat actgaaacat attcctgaaa gatcttttat tatttggcgt tgagctccca
      361 tttgggtgaa tacaaaacag acttggtttt gtttgccata ctttccaaat ttcatcacca
      421 atttcggtta ctaccgtctt aaatgcactg gcagatgaat tgttcatttg cgttaatcta
      481 tttccgacat acatatatat ttgtggtagc gacatagaat aacgattgct attaagatag
      541 aaatctgatt gtggttcatt ctgatggcga cgctcaccat aaactaaaga aagtacagcc
      601 ttaactgctc tctttgatga accatcggca gagaaaggaa ttaaaaggcg ctctgctgcg
      661 gttaaagcta actctgtata aatagagaag ctagggttgc agtcaatgaa tacgcagcta
      721 tcttcattat tccatgagtt gcaaatgtca ctgattaaat cattaatcca gaggtgaaca
      781 gtacgccatg catcatgggg acctggaaat gatgccccaa caactcttga tgcttggagt
      841 tctaattgtt catcgccagt gacaagatat agattatttg gaactttact attgtgtgta
      901 gatacattta ttacatagct agaaccagtg ttggtaggaa tatatggact tctgatccta
      961 tcctcaatat atccagatat tgtctttctc tgtgttgctg aatgaatctg gtttagtgtg
     1021 ttttcgccat ttatcattcc accaagtaac atagaggacg agttcgcttg tgggcacagg
     1081 tcaaccacca ggaccttttt agtagggttc tgtctggcat attcagaggc taactgaaaa
     1141 gtaaggtaac ttttccctac accacccttg ttattccata cggcatagat cgccatctat
     1201 aactcctaat ctagttttgt tgttagctta gattcagggt actagagatt attgttctac
     1261 tgttagtttc tttcagctga ctgccttttt ttggaacgat attcagtcga aggggttggc
     1321 tggctcacca tgacttatct ggagttgtcg aattccgatt aaaaggatct tcttgagatc
     1381 ctaacctgcg ggatgtaatc ttttgctctg taaacgaaaa aaccacctgg ggaggtggtt
     1441 tttcgaagga tcagtaagtt ggggaacttc tgaaccgtgg taacagggtg tacaagaccg
     1501 ctgccaccaa atctgtcctt tcagtttagc tgtagttagg ctgtaacttc aagaactctg
     1561 caccagtaat tcttgtacac cccttaccag tggctgctgc cagtggcgtt ttgtcgtgcc
     1621 ttccgggttg gactcaagtc gaaagttacc ggaacaggcg cagcagtcgg actgaacggg
     1681 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     1741 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     1801 caggagagcg cacgagggag ccaccggggg gaaacgcctg gtatctttaa agtcctgtcg
     1861 ggtttcgcca ccactgattt gagcgtccgt ttctgtgatg cttgtcaggg gggcggagcc
     1921 tatggaaaaa cggcaacgcc gcggccttct tttgttcctt ctggccctct gtgctgtcgt
     1981 cttatgccct tatacgtgag ttactaccgc tcgccgcagc cgaacggcag gagcatagcg
     2041 actgagtgag cgaggaagcg gaaaagagac cagttttaca ttgagcactg acgctctgag
     2101 gccctttatg actaaacaac agaaaacagc gctgaatatg gctaaattca tccagaatca
     2161 gtctctgctc ctgctggaga agcttaatga gcttgatctc gatgcggagg ctgacctttg
     2221 tgagaaactc cacgatgatg cggagcatct tttccgtaca ctgtcctcga gactggatgc
     2281 attacaggat ggtaactgat ggccagagtg tctattagtg aggctgcccg actgacgggt
     2341 aagagtagaa caacactgca caggttaatt aaagcaggtg acctgtccac ctgtagcggt
     2401 gaacgcaatg caaaaatgat agatacttca gagcttttgc gtgtctttgg cccctttgag
     2461 caaccgaggg gtgaacacca ttctggacag gtctctgaac agcgtgatac agcctttgct
     2521 gaccagagtg aacaggtgat tcatcatctg aaacaggaga ttgaacacct gaatacctta
     2581 cttttgtcca aggattcgca tattgacagc ctgaaacagg ccatgctcct tcttgaaaat
     2641 caccaggcaa aattaactga atccccggcg ccatggtgga aattctggaa aaaatcctga
     2701 tcccgccatg cttgagtttt cctctccggc catcccgggg agttcctgaa ccttcccaaa
     2761 aatcgtcaga tctctgaacg ctcctcacca cgaaaaatac ccacacacta cgcaaaatta
     2821 aaattttgca gatttgtatt taatatcagt ggtttacttt cctgaaattt cgttttttaa
     2881 attttattta tatagatccg actctatgac gactcgtcat agagtccccg gcgagtcgtc
     2941 atagagtcgg taacgactca ggactataaa aacaataagt taataacgac ccgactctag
     3001 acacgactcc ccgtttggtt gttttttaga cgaggcgact ctgggtacga ctctagtgaa
     3061 gagtcgccgg tgaatcgtgt ggtgagtcgt ccgcgacttg ccatcacgtc attgccggct
     3121 gtcgacgtac ccgattcctg aacgtgctga aaacgggttt tccggctgcg ccggcaacgc
     3181 ctcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggaggaga agaacgccgg
     3241 aaacaggact tcccctggct gaatggtgtg aatttccgcg ctatgcactt gcgcgcatac
     3301 tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg
     3361 cctgttttac cggtttcccg tcagaaacgc cctgagggcc tctcaggcgg tgctgataac
     3421 ggatgttatg gtaaattcgt ctgtgaagta ggtagacatt tctaaagcta gttttaatag
     3481 ccaagtctct tcaattcagt cgagaggcgt ttgaggctta catcaagatt gcttttattt
     3541 tgttttaact cttttttcat aaactcagtg tgatgtgcta gctcagggtt atcagttgag
     3601 cgagtcacgg gaaaatcatc actggatgaa agtcgcccat ttttactgta gtccatggtg
     3661 atacagatca gagttgcaag gtcgattacc ctatgaagag gcagctcttc tgattgtctt
     3721 gaccattttt ctcctgtgtt tcgccaaacc tttgccgaaa gctcagtcca tccaggagtg
     3781 ttccattgag caatacctac agatagcccc atagcatcag tgtcatctgc gtgtgggccg
     3841 tcaatgcggt cgtaattttc gacttgaata attggtttat gcttcaaatg cgcgggtatt
     3901 ttcatcagtt cgtccttagg taatgtttgt gattattaaa ttacaccgtt tttctaatta
     3961 taaaactcac taaatgtaag taaatattaa tatctatttt tatcatgtgc ttgtgatttt
     4021 gtttttcgaa ttttatgttt tttgtttgga gagtagcggt gtttttttgt gtcaagtgtt
     4081 atgataagcc gtacgttttt ttctggttta tagttcggtt attcacagca atgggcgtgg
     4141 ttttctatga ctacagataa cgttaggaat attggcgact ataaaaagaa agatgtcggg
     4201 gtggatgctg actgtaatcc agtctcagga aacacaccac cagtcagact aagtgagaaa
     4261 cttagaaatg gaattcctcc tggtttgaaa actcagatgc gccaacaaat gaggaaaaaa
     4321 catgggctct aacaataatc agaataaaga tctgcaggat aagcaagctg atcttttgga
     4381 ccaaacgtat actcttggtt ggatggatgg ctatgccgaa gggtatgatg atgctattga
     4441 agatactcag aacgctttga ataaactgga a
//
