LOCUS       NZ_CP113034            17606 bp    DNA     linear   CON 14-JAN-2024
DEFINITION  Staphylococcus aureus strain Akali plasmid unnamed3.
ACCESSION   NZ_CP113034
VERSION     NZ_CP113034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31523637
            Assembly: GCF_026625365.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17606)
  AUTHORS   Huang,Y.-T. and Liu,P.-Y.
  TITLE     Whole genome sequencing of methicillin-resistant Staphylococcus
            aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17606)
  AUTHORS   Huang,Y.-T. and Liu,P.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2022) Computer Science and Information
            Engineering, National Chung Cheng University, No.168, Sec. 1,
            University Rd., Min-Hsiung Township, Chia-yi County 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 306x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:23:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,172
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 2,979
            CDSs (with protein)               :: 2,979
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 59 of 113
            Pseudo Genes (incomplete)         :: 51 of 113
            Pseudo Genes (internal stop)      :: 23 of 113
            Pseudo Genes (multiple problems)  :: 19 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..17606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Akali"
                     /isolation_source="environmental"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed3"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.23321 N 120.9417 E"
                     /collection_date="2022-06-01"
                     /collected_by="Po-Yu Liu"
     gene            complement(<1..306)
                     /locus_tag="OS080_RS15730"
                     /pseudo=""
     CDS             complement(<1..306)
                     /locus_tag="OS080_RS15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(543..638)
                     /locus_tag="OS080_RS15735"
     CDS             complement(543..638)
                     /locus_tag="OS080_RS15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000889979.1"
                     /translation="MLEILVHITTTVISGCIVALFTHWLRNRNDK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1116..1724)
                     /locus_tag="OS080_RS15740"
     CDS             complement(1116..1724)
                     /locus_tag="OS080_RS15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017432088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000793183.1"
                     /translation="MKNLKSFFIKNTLVLTSTALLFSSFEPVVHAAENKEVVKTVENDN
                     VEFNELLEGQEYIWEVLSRDDEGFKMFLDEQRAFNPNFDNDVLLAKQEGALNSSVFANN
                     SMTLNSNKPTPRGPIGATLKAIKALSPSLRHGGTAISWIIKPLSRKHAVLVKKYSRKMS
                     NAIDRLDKSTKSAVENAFVKAGIPRSDAKTLTYIVFLVI"
                     /name="hypothetical protein"
     gene            complement(3231..>3335)
                     /locus_tag="OS080_RS15880"
                     /pseudo=""
     CDS             complement(3231..>3335)
                     /locus_tag="OS080_RS15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(3395..4069)
                     /locus_tag="OS080_RS15745"
     CDS             complement(3395..4069)
                     /locus_tag="OS080_RS15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISSau6 family transposase"
                     /protein_id="WP_001105943.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGIYVHHS
                     TIYRWVQEYAPILYQIWKKKNKQAYYKWHIDETYIKIKGQWNYLYRAIDADGHTLDISL
                     RKKRDNHSAYTFIKRLIKQFGKPQMIITNQAPSTKVAMSKVIKDFKLTPNCHCNSKYLN
                     NLIEQDHRHIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRFLQIYGFSPCRVISIML
                     AS"
                     /name="IS6-like element ISSau6 family transposase"
     gene            4106..5017
                     /locus_tag="OS080_RS15750"
     CDS             4106..5017
                     /locus_tag="OS080_RS15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_267841570.1"
                     /translation="MSNFATEPNVSYADILEILKIKKAYNVKQCGNVLEFKPTDEGYLK
                     LHKTWFCKSKLCPVCNWRRAMKNSYQAQKVIEEVIKEKPKARWLFLTLSTKNAIDGDTL
                     EQSLKHLTKAFDRLSRYKKVKQNLVGFMRSTEVTVNKNDGNYNQHMHVLLCVENAYFRK
                     KENYITQEEWVNLWQKALQVDYRPVVNIKAIKPNQKGDKDIESAIKETSKYSVKSSDFL
                     TDDARKNQEIVSDLEKGLYRKRMLSYGGLLKQKHKILNLDDAEDGNLINTSDEDKITDE
                     EAKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            <5927..6028
                     /locus_tag="OS080_RS15755"
                     /pseudo=""
     CDS             <5927..6028
                     /locus_tag="OS080_RS15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(6063..6203)
                     /locus_tag="OS080_RS15760"
     CDS             complement(6063..6203)
                     /locus_tag="OS080_RS15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001199886.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVRFCCKVK
                     K"
                     /name="hypothetical protein"
     gene            complement(6394..6741)
                     /locus_tag="OS080_RS15765"
     CDS             complement(6394..6741)
                     /locus_tag="OS080_RS15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119692.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDGI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(6760..7376)
                     /gene="cadD"
                     /locus_tag="OS080_RS15770"
                     /pseudo=""
     CDS             complement(6760..7376)
                     /gene="cadD"
                     /locus_tag="OS080_RS15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /name="cadD"
     gene            complement(7557..7679)
                     /locus_tag="OS080_RS15775"
     CDS             complement(7557..7679)
                     /locus_tag="OS080_RS15775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466482.1"
                     /translation="MFFYEDDDFDEMEEKYDYHEDKKIDDAFMPRKFIDVEKNP"
                     /name="hypothetical protein"
     gene            complement(7760..7948)
                     /locus_tag="OS080_RS15780"
     CDS             complement(7760..7948)
                     /locus_tag="OS080_RS15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001030803.1"
                     /translation="MNKDTMLDKGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(8528..8674)
                     /locus_tag="OS080_RS15785"
     CDS             complement(8528..8674)
                     /locus_tag="OS080_RS15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001092935.1"
                     /translation="MNTKILTGMTGSSLERKINNFINDNQIEVIDIKFSSSVFYFGVMI
                     IYK"
                     /name="hypothetical protein"
     gene            complement(8679..8882)
                     /locus_tag="OS080_RS15790"
     CDS             complement(8679..8882)
                     /locus_tag="OS080_RS15790"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001796115.1"
                     /translation="MYRKRMLSYGGLLKEKHKILNLDDAEDGNLINTSDEDKTTDEEEK
                     AHSITAIWNFEKQNYYLKNLRS"
                     /name="protein rep"
     gene            complement(8997..9941)
                     /locus_tag="OS080_RS15795"
     CDS             complement(8997..9941)
                     /locus_tag="OS080_RS15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_267841568.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSIQNEWVDRNNNIYFIFSNKHLCEYLGYGEQKIIKLKKELVNFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASLTKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTELNNTN
                     YIENEDNDMYDMNDIISNTLINNNHHSNHTNHQKTEFNNDALKFQVLEELPQQLQDYLS
                     KFEIREIRIIKSVLLKGKKSFNTSHDTYYRLEDVEFEIVSVLKRFKAMLLQKNETVEAM
                     QGYLMQSIKAELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            10385..11155
                     /locus_tag="OS080_RS15800"
     CDS             10385..11155
                     /locus_tag="OS080_RS15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848988.1"
                     /translation="MKTIKMVADELNVTKQTIVNNAKNLNISFKKENGINYINDNDCLK
                     IIEKITKKERTMQNKESIKKERFNENTAEFEKHIYNNSNGFESLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLENQLEEERQLN
                     YSFDTATNDRQSINPQEATFTEEYQNSNQQQKKIQPIEAELNHKGITKAKKDDSSNEDV
                     LKRDDKLSEEIESGPEDREVETPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(11471..12031)
                     /locus_tag="OS080_RS15805"
     CDS             complement(11471..12031)
                     /locus_tag="OS080_RS15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(12236..13780)
                     /gene="qac"
                     /locus_tag="OS080_RS15810"
     CDS             complement(12236..13780)
                     /gene="qac"
                     /locus_tag="OS080_RS15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_000622774.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSIMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            13958..14524
                     /gene="qacR"
                     /locus_tag="OS080_RS15815"
     ARG             13959..14521
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(14657..14842)
                     /locus_tag="OS080_RS15820"
     CDS             complement(14657..14842)
                     /locus_tag="OS080_RS15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(14847..15185)
                     /locus_tag="OS080_RS15825"
     CDS             complement(14847..15185)
                     /locus_tag="OS080_RS15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            complement(15431..16039)
                     /locus_tag="OS080_RS15830"
     CDS             complement(15431..16039)
                     /locus_tag="OS080_RS15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            complement(16165..16743)
                     /locus_tag="OS080_RS15835"
     CDS             complement(16165..16743)
                     /locus_tag="OS080_RS15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690625.1"
                     /translation="MKIGYARVSTGLQNLNLQEDQLNQYGCEKIFSDHISGSKSKRPGL
                     DKAIEFARAGDTIVVWRLDRLGRNMEDLITLVNELNNRGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNGTPI
                     KTIAEQWKVSRTTIYRYLNRLNNQENKDK"
                     /name="recombinase family protein"
     gene            17115..17483
                     /gene="cadC"
                     /locus_tag="OS080_RS15840"
     ARG             17116..17480
                     /name="cadC"
                     /gene="cadC"
                     /gene_name="Cd(II)/Pb(II)/Zn(II)-sensing metalloregulatory
                     transcriptional repressor CadC"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM/LEAD/ZINC"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17476..>17606
                     /locus_tag="OS080_RS15845"
     CDS             17476..>17606
                     /locus_tag="OS080_RS15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003267.1"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing protein"
                     /protein_id="WP_267841572.1"
                     /translation="MSEQKVKLMEEEMNVYRVQGFTCANCAGKFEKNVKKIPGVQDA"
                     /name="heavy metal-associated domain-containing protein"
     ARG             complement(12240..13780)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          5088..5248
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.386"
                     /coverage="100.0"
                     /evalue="3.17e-70"
     Typing          4130..5017
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.288"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8690..8867)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.179"
                     /coverage="95.0"
                     /evalue="3.73e-65"
     Typing          complement(8998..9941)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.471"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatatctaat gtatgtccct ctgcatcaat ggcacgatat aaatagctcc attttccttt
       61 tattttgatg tacgtctcat caatacgcca tttgtaataa gcttttttat gctttttctt
      121 ccaaatttga tacaaaattg gggcatattc ttgaacccaa cggtagaccg ttgaatgatg
      181 aacgtttaca ccacgttccc ttaatatttc agatatatca cgataactca atgcatatct
      241 tagatagtag ccaacggcta cagtgataac atccttgtta aattgtttat atctgaaata
      301 gttcatacag aagactcctt tttgttaaaa ttatactata aattcaactt tgcaacagaa
      361 tcttttatag tatcaaagaa aattaataat tctaattata gagttacact tacgtttctt
      421 atgtttttgc attcctctac taaccagtgt tataatatga ttaacaagaa ttctagcgtg
      481 aatacaccaa tcccctcact attgccgtag tgaggggatt ttttaggtgt gactatagtc
      541 gcctatttat cgttgcgatt gcgtagccaa tgcgtaaata acgcaacaat acaaccactg
      601 atgactgtgg tcgtgatgtg aacaagaatt tctagcatga ataacacctc ctctctgcgt
      661 ctaaatttga cgcctgagag ataggcgact ctattattat accatctgtg aatatacaag
      721 catatctact tccgataagt cgtcttatgt tatataactt cgttttttat agtatcaaga
      781 caaaagaaga aacgcagttc aaaatcatca cctaaattca ttaatctaaa gaaaaaacgg
      841 tgtaataaat agtgaatatt atagtctatt aaagaataga tttaaaaaca ctctaaaagg
      901 tgcattaaag atttgtaaaa ctttaatata tcctttttag gtgaaaactt atgataattg
      961 taggttctct cattatctat gattattaat tttaactgta tctagaaaat catcacccca
     1021 atcacaaatt gaaaatacta ctttatcaag ataagaaaaa aaaaaaaaac ttgccctaag
     1081 attttataaa atcttagggc aagtcagctc aatatctaaa taactaaaaa tacaatataa
     1141 gttaaagttt tcgcatcact tcttggtata cctgctttaa caaaagcatt ttcaacagct
     1201 gatttcgtag acttatctaa cctatcaatt gcattagaca tctttcttga atattttttt
     1261 actagaacag catgttttct acttaatggt ttaatgatcc aagatatagc agttccacca
     1321 tgtctcaaag aaggtgatag agccttaata gcttttaaag ttgcaccgat aggacctcta
     1381 ggagtaggct tatttgagtt taaagtcata gaattattag caaatactga agaatttaaa
     1441 gcaccttctt gttttgctag taaaacatca ttatcaaaat taggattaaa agctctttgc
     1501 tcatctagaa acattttaaa tccttcatca tctctagata aaacttccca aatatattct
     1561 tgtccttcta acaattcatt gaattctaca ttatcatttt ctactgtttt aacaacttcc
     1621 ttattttctg cagcatgaac tacaggttca aaactagaaa ataataatgc agtagatgtt
     1681 agtaccaaag tatttttaat aaaaaaactt tttaaattct tcatatgact tttatcctcc
     1741 aattataaat gttaaaattt tttattatta aatattatat acataaaagg aattaaatta
     1801 ttatgaaaaa agtaaacaaa gagtacttat tgaactttat aattgtaacg ttcttactcg
     1861 gaataataat catacctata tccgtattag tattctcaat accatttttt agcataatag
     1921 gtttccaatc attagcagat aatttaaata taagttcttt aataacaata aattggtatt
     1981 caaataatat gttttataac attggctata ttgcattatt ctttatagta acttatctaa
     2041 tatttatttt tcttgaactt tttataatat ttttacatat aaaatacaaa aagaaattta
     2101 ataatattat cttattcata ttctttataa ctgctaatat gatcgtatta aacttaataa
     2161 ttttgaagat tttctacaac attcatataa acttatattt tcaaattttc atttattctt
     2221 tatgcttcta tcttttattt actcataaag agatttacaa aatatttgaa gaaaataaaa
     2281 gtaaaaaata aaaaaataaa aaaataaaaa aattaatctc tcaaaaagtt ttctcggcat
     2341 aaaagtacct ctaactttta tacataaagt catgagcata agggaaagtg ccaccacttt
     2401 tatattttgg cagacccata acgtggtgaa ctcggcagtg ccgagttatt tttcctagaa
     2461 aaaaagtgac atcacttttt taactcatat gtataaaaac cataacgtta tgtatacata
     2521 caggttacgt atacatatac tttatgtata taaaacatac acaaaagaca tgcatcatac
     2581 acatcaattt ttttaaaaat acatgtcttt ttgatatata ttttttttgc gttttaagag
     2641 gtcatttaga gcattctcta attttgagta caaaaatacg taatataaaa aaatcgtcct
     2701 tttaaaacct ttttaggaca ttttaaacac atttttatga tttggatcaa tgtagaataa
     2761 atgtcataaa cgtatagaaa aaatacgtcg tcatttctaa gtacagataa atttctgatg
     2821 cagtactaaa ttatggggaa acctacctgt aaaaaataaa aagattacta tattttaatt
     2881 ttattttttc tgttcgtttt ttcgacacca ggacgggcga tttttacgta ctgcccattt
     2941 acatgcaaat ttaaaaatta gcaataaatc gttccgaatt tgcattttaa atgggcaaac
     3001 gtgccgtttg atcacatagt tcgtgtagcg acaaaaagag tataacgaaa gtataataag
     3061 cgtataaaaa aagtgtaatg atagaaaata gcattatact tttttagtcg ttcatcgctc
     3121 atcagaacaa caaataaatt tttaagaaat aaattttgag agttgaccac ccacgttctc
     3181 cgtaaactac aaatgtgggt ggtcaactct caaaatattt ttatattttt ttattgttct
     3241 tcttcggtaa gttcgtgaag ttcacttgcc aggtactctt tctgattcca aatataataa
     3301 atttggtaaa ggtattcagt tggatcgatt tcttggttct gttgcaaagt taaaaaataa
     3361 gtgtctaacc ttatgtttaa aataatactt cgtattaact agctagcatg atgctaatta
     3421 cacggcatgg cgaaaacccg tagatctgaa gaaatctgcg gttcttttta tatagtccat
     3481 aaatacattc aatgcctttg atcgtattct ttgccgtatt gatactttga taacttattt
     3541 tccttacttt gatatgacga tgatcttgtt caatgagatt atttaaatat ttagagttac
     3601 aatgacagtt gggggtaagt ttaaaatctt taatcacttt ggacattgcc accttcgttg
     3661 aaggggcttg gtttgtaatt atcatttgag gtttgccaaa ttgtttaata agacgtttaa
     3721 taaacgtata tgctgagtga ttatctcgtt tcttacgtaa cgaaatatct agtgtatggc
     3781 catctgcatc aatagcgcga tataaataat tccattgccc tttaattttg atatacgtct
     3841 catctatatg ccatttataa taagcttgct tatttttttt cttccaaatt tgatacaaaa
     3901 taggagcata ttcctgtacc catcgataaa ttgttgaatg atgaacatag atgcctcgtt
     3961 ctctcaatat ttcagatata tcacgataac ttaatgcata tcttagatag tagccaacgg
     4021 ctacagtaat gacatctttg tcaaattgct tatatctgaa ataattcatc ctctgcacct
     4081 cattttttgt tgactattat actatatttc taactttgca acagaaccta atgtaagcta
     4141 tgcagatata cttgaaattt taaaaataaa aaaggcttat aacgtaaaac aatgtggtaa
     4201 cgtcttagaa ttcaagccga ccgatgaagg ttatttgaaa ttacataaga cgtggttttg
     4261 taaatcaaag ctatgtccag tttgtaattg gaggcgtgct atgaaaaata gttatcaagc
     4321 tcaaaaagtg attgaagaag taattaagga aaagccaaaa gcacgttggt tgtttttaac
     4381 actttcaaca aaaaatgcga tagatggaga tactttagaa caaagtttga agcatctaac
     4441 taaagcattt gataggttga gtagatataa aaaggttaaa caaaatcttg ttggatttat
     4501 gcgttcaaca gaagttaccg ttaataaaaa tgacggtaat tataatcagc atatgcacgt
     4561 tttgttatgt gttgaaaatg catattttag aaaaaaagag aattatataa ctcaagaaga
     4621 atgggttaat ttatggcaaa aagcattaca agttgattat cgacctgttg taaatattaa
     4681 agcaattaaa cctaatcaaa aaggcgataa agatattgaa tcggcaatca aagagacatc
     4741 aaaatattcg gttaagtcat ctgatttttt aactgacgat gcccgaaaaa atcaagaaat
     4801 tgtaagtgat ttagaaaaag gtttatatcg aaaacgtatg ttaagttatg gtggattgct
     4861 taaacaaaag cataaaattt taaacttaga cgatgccgaa gatggtaatt taattaatac
     4921 aagtgatgaa gataaaataa cagatgaaga agcaaaagca cattcaatta cggcaatttg
     4981 gaattttgaa aagcaaaatt attatttaag acattagtga tgactgatgt cttttttatt
     5041 gattttttgt aaaaaagtac tgtcttattt ttgtgacaaa tgctgtatgt agtgtcacct
     5101 gtgacaaacg caatatattg tgtcacaaaa gtgtgacact acagcttttt atgatatcac
     5161 tttaaaatta cttaaaaccc ttggaatgtc tggctttgcc agacctatta tttttgaatg
     5221 atagcaaatt ctccttatgc tcttacggag ttcttagaga aaaattgaaa tttcttgatt
     5281 tttgataaaa acgccctgca ggaatttaga aaaacatggg gaagttttaa aagattttat
     5341 gctcattttt aatttgcatt taaatgggca gtgttaaaaa attagtcact ggttttgctg
     5401 aaatttttgt ttgtgaaatt accatatatt tatttgactc atatttgcgt tttaagagct
     5461 tgtatagatt tttaagtaga agactataag atttacctct aaatctttaa aataccctct
     5521 taaaattcaa aataaaggtg tttataattt gaataagtcc tgagataaag ttttttaaaa
     5581 ggagtggttt tttgaagagg tctttattta ttttacaaat tattcttggt atttttttat
     5641 tgttttttat ttataagcaa atgaatcaaa atcatttaga tacttggttt attctttata
     5701 cagtcttttt tatatttttt tcatcgtggt ttgataaaca ttataaaaaa agttagacga
     5761 cgcatttatg ccgagaaaat ttattgatgt tgagaagaac ccttaactaa acttgcagac
     5821 gaatgtcggc atagcgtgag ctattaagcc gaccattcga caagttttgg gattgttaag
     5881 ggttccgagg ctcaacgaca ataaagcaat tggaataaaa tacacaggca ttgaatgtat
     5941 ttatggacta tataaaaaga accgcagatc tcttcagatc tacgggtttt cgccatgccg
     6001 tgtaattagc atcatgctag ctagttaata caaagtatta ctttaaacat aaggttagac
     6061 acttattttt taactttgca acagaacctt acattatcta ttttcttttc tctccatggg
     6121 cgttgttttc cacttttcgt catatctttt aacgttttat tatcttgatt ttcggttata
     6181 cttctagtag tattatattg catataaaaa gaactccagt attgatttag attagacacc
     6241 ttaatcatat tactggagtt tttttatgtc aaattttatt ttccccaagc aaaacagtat
     6301 gaaacgccgt catatcaacg cttcaaaagg attttagaaa agaacgttat ttctatatag
     6361 tatcaagaca agaagaaact cgtttcactc gtttcaaaaa ccctcatgat ttttagagaa
     6421 aaactctctt attccatcgt ctttaataaa ataatatgcc atctttccat ctttataaaa
     6481 atcaagtacc tcatttttat ataaaagcct taaatgatgt gaagttgaag caacactcat
     6541 tttcaatatc aaagaaatat cacaaacaca caactcatct tctttaatca aagataatat
     6601 gattttcaat ttcttctcat cacaaatttt ttctaaaatg ttaagtaatt tcttagattt
     6661 atcatcttct aaaaaactta aagcattgtt aactttatcc tcatgtacac agatcacatc
     6721 acaagtattt tcataactca tataaaatca caccctaaat taaaatataa atcctaaaat
     6781 tgtttgaata gtgtcatttt caataataat aaataaacct aaagctatat aaataatagc
     6841 cataatccaa cgactaaatt tctcaacaat ttctccaatt cctgggatat tagctaattt
     6901 ttgtgcagta aacaccaaga agaaaattaa aattaaaaag acaaacagag taagtaataa
     6961 attagtaaca cttaatgtca caaaatacgg aacaaataaa ccaatattat cggcgccaca
     7021 acttgctatc gtaacaattg caaccgtacc aactaattta gacaatcctt tttcattcag
     7081 ttctttttta gctctctttt ctccttcaca atcacataaa tagccacttt aattccaaga
     7141 taaattggta ttaaacccaa taaacctaat atccacttct ctgaaacata atttaataca
     7201 aaagctaaaa acaaactaac taatattaaa ataatagacc ctaaatattg accaatataa
     7261 atatctctat attctttttt agtctttgct ctagcaaaaa atattaataa aattactaat
     7321 aaatcaaatg ctgtagcaat ataaagaata gcagcagtga caaccgtttg aatcatacat
     7381 acacctcatt caaaaatatt tttgaatgta ttataactaa tatttttaaa taatcatagg
     7441 tgctttattc caattgcttt attgacgttg agcctcggaa cccctaacaa tcccaaaact
     7501 tgtcgaatgg tcggcttaat agctcacgct atgccgacat tcgtcttcaa gtttagttaa
     7561 gggttcttct caacatcaat aaattttctc ggcataaatg cgtcgtctat ttttttatct
     7621 tcatgataat catatttttc ttccatctcg tcaaaatcat catcttcata gaaaaacaaa
     7681 aagtaagcaa caacaccaat aggaataaaa aatagtgtga aaacaagaag aaactcgttt
     7741 caaaatcccc tcaaatattc taattattct ttttgaaatt cataacttta acaaccaaat
     7801 aaatagcatt tattcccatc aaaataacat acggaattaa tatatataaa ttttccaaat
     7861 gagatataaa aattaatcca agtaaaaaga aaacatttaa aatagttgct ataacgtaac
     7921 ctttatctaa cattgtgtct ttattcaaaa taaaaactcc tttttaaaaa actttatcac
     7981 aagacttact caaattataa atacctttat tttgaatttt aagggggtat tttaaagatt
     8041 taggggtaaa tcatatagtt ttctatctaa aacctacata atctcttaaa acgcaaatat
     8101 gaaccaaata catatatgct attttcaaaa acaaaatttt aaataaaacc aatgactatt
     8161 tttttaaaca ctgcccattt acatgcaaat taaaagttag cataaaatcc tttaaaactt
     8221 cccccatgtt tttctaaatt cctgcaagac attttttatc aaaatcgaga atttttattt
     8281 tttctctaag aactccgtaa ctccgtaaga gcataaggga aatttgaccc catgtataca
     8341 tggggtatct ggcaaagcca gacaattcaa agcttcaaca atatttttat gtggggtcat
     8401 aaaaagctta agtgtcacac ttttgtgaca caatatattg catttgtcac aaaaataagc
     8461 cagtactttt ttataaaaaa acagtaaaaa aagacatcag tcatcactaa tgtcttaaac
     8521 tctttaatta tttataaata atcattaccc caaaataaaa tacagaactt gaaaacttaa
     8581 tatcaatgac ttcgatttgg ttatcattaa taaaattatt gatttttcgt tctaaagaac
     8641 ttccagtcat tccagttaaa attttagtgt tcatattttt aactccttaa attttttaaa
     8701 taataatttt gcttttcgaa attccaaatt gctgtaatcg aatgtgcttt ttcttcttcg
     8761 tctgtcgttt tatcttcatc acttgtatta atcaaattac catcttcggc atcgtctaag
     8821 tttaaaattt tatgcttttc tttaagtaat ccaccataac ttaacatacg ttttcgatac
     8881 aaaccttttc tagatcactt aaaatttctt gatttttttc gtcgtcatca gttaaaaagt
     8941 cagatgattt aacggaatat tttgatgtct ctttgattgt tataaaatca tttgaattat
     9001 tgattaaaga tattatattg tttcatgttt ttacggcgca tataaagggc atgtgtttct
     9061 tcaagttcag ctttaatgga ttgcattaaa taaccttgca tagcttcaac ggtttcattc
     9121 ttttgtagca acatcgcttt aaaacgtttc aatacactta caatttcaaa ctcaacatct
     9181 tctaaacgat aataagtatc atgtgaagta ttaaatgact ttttcccttt gagtaatacg
     9241 cttttaataa tccgaatttc tctaatttca aatttactta aatagtcttg aagttgttgt
     9301 ggcaattctt cgagtacttg gaacttaagg gcgtcattat taaattcagt tttttgatga
     9361 tttgtatgat tcgaatgatg attattattt attaaagtat tagatataat atcattcata
     9421 tcatacatat cattatcctc attttctata taattagtat tatttaactc agtatcatta
     9481 gactgtgaat ttggaagttc ttgaccactt aaattcacag ttctagaact tccaaattca
     9541 ttgttctgga actgtgaatt tggaagttcc ttggtaagac ttgcttcaat atcataattt
     9601 ggttttaata aatataatct atttggttta ttaaggccaa cacgctcttg agttaataaa
     9661 ttaaaattta ctaactcttt tttcaatttt atgatttttt gttctccata acctaagtac
     9721 tcgcacaaat gtttattact gaaaataaag taaatgttat tatttctatc tacccattca
     9781 ttttggatgg ataattcgaa acgatctttt aataatgcat acataatttt ggcttctgct
     9841 gacattttct taaaatatgc atcttcgaac aaatatttag gaagttggta aaatctttca
     9901 gtttttactt gtgataaatt gtatttctca aaattgggca taataaaaac ctcatttcca
     9961 ctttttggat agaggtaata aaacaatttt tacgtaggtt atatgttttg tacttgcact
    10021 tatatgatta gatatatata atacaagtat caaaaaatta tataaacaga cgaatgcgaa
    10081 tcgttttatt accgagagtc gatgctacgc caatagtatc gactctttta atattcacaa
    10141 agtaatatta acacttttaa gattgtatat aaagcttttt aagtttagtt gaaagaacat
    10201 tgtaacttta aaatgataaa aattcaccac tttagtacct aattagtcac ctatagaagg
    10261 ttttaacggc taattaggta ctaaagtagt gaaataggta gtaaatttag atgtcgaaag
    10321 atgaaaaaat atttgttagg tggtaaaatt taggtaccaa tttatgtacc taaggatgaa
    10381 tgttatgaaa actattaaaa tggttgctga tgaattgaat gtaactaaac aaactattgt
    10441 taataatgct aaaaacttaa atatatcttt taaaaaagaa aatggaatta attatattaa
    10501 tgataatgat tgtttaaaaa ttatagaaaa gatcactaag aaagaaagga caatgcaaaa
    10561 taaagaatca ataaaaaaag aaagatttaa tgaaaatact gcagagtttg aaaagcatat
    10621 atacaataat tcaaatggtt ttgagtcatt aaaaacaaaa gtaaatgaat tagaaaagca
    10681 aatagaaatc tttgaaacta gagctaaaaa tgacgaaaaa tatattgaga atttaactaa
    10741 acaactagat caacaaaata gcaatgttaa tacattaaat aaactgttgg agaatcaaca
    10801 aatattagct ttagaaagta ataaaaagat tcaaaaacta gaaaaccaat tagaagaaga
    10861 aagacagctt aattactctt ttgatacagc gactaatgat agacaaagca ttaatccgca
    10921 agaagcaact tttactgaag aatatcaaaa tagcaaccaa caacaaaaga aaatacaacc
    10981 aatagaagca gaattaaatc ataagggcat aactaaagcg aaaaaggatg acagttcaaa
    11041 cgaagacgta ttaaagagag atgataaact atctgaagaa atagagagtg gacctgagga
    11101 cagggaagta gaaacaccta aaaaggggtt ttggagtcgt ttgtttggta gctaaaataa
    11161 ttaattaggc actaaaagtt tataactttt agtgcctttt atatagtgag aacttctggt
    11221 aatcatcagt tatgttaaac tctaacaaaa actaaattat cagaagaatt atctagggta
    11281 ttaaacatag gtaaatcaat tttctatcgc caatagtata aacatcatcc atattaatct
    11341 gaagatagct acataattct ttaacagcat agcctttatt tgtataaggc gccataaact
    11401 ctattaaata tggtttgttt tgaggtttct atgtttaatc atttaatttt tcattatata
    11461 aatcgaataa ttataaaaag tcatcatact ttttattttt taaaaatatt tgaaaattta
    11521 atagtaatcc aattataaga tcaaatatca ttatcattat aatcaaggca actataatca
    11581 ctgtgacact cgttacataa caagctagta atctccagat aaaattgaca ttttcaccaa
    11641 ttccataact taacatatta caaagtagta aaagaataat aatacctata ctgtaaatta
    11701 aaattgtcag tgtatatttt ttaataactc tattaggaaa caatagttca tctattaggt
    11761 tctttttcac cagtatctct cgttcaattc tatctttgtc acagattttg attttactat
    11821 tttttatttt ttcctctaat aaaataacct cgaacgatct gctaattttt aataaatatg
    11881 caggaaaagc taatgttaag cataatgtaa ttaattcatt tacattgttc atgtaactta
    11941 taaaatagat gaaagttgct actagtgctc ctaaaaatat agttatatta agttctacat
    12001 caaatatttt aaaaatatct gctctattca tcagttaatc atcctttcaa ggtttattaa
    12061 tactaataaa ttattagata taggtatatc atattattaa tttaagaaat tgtcttttat
    12121 aattttactt tatattaaaa aagtagaacc atttaaaatt aatggttcta ctttttaact
    12181 attactaatt ttaaaaataa actaatctac aatatctaaa aatatatgtt tagtactatt
    12241 tttgtttagt tatatcaagt gatttgggaa ttaacaaata gacaactatt gaaatgataa
    12301 tcatgataat cccacctact aaagcagttg ctacaaaagc atcattaaat gatgttacag
    12361 cctcgtttgc aagctgtttt atccccgtag ctttagctac ttcgacagcg cccactacag
    12421 attcttcagc tacatgagct aaatctccaa ctataccttt tgatgaaaaa gatgaaatat
    12481 ctaaaaatac acgataaagc atagaagata ggctaccaag tactgctaca ccaaaaacat
    12541 ttccaaggtc atacatagac tcttcaacag cagctgcatt acctgctttt gatgtaggtg
    12601 tttctaacat tattagagca gatgcaactg ctagtgaagc cataccagct ccaactaaaa
    12661 ttaatgctaa agccattata gaatatgata atggatgacc aaagaaatac ataataaaca
    12721 tgccaatggc tgcaattcca attccggaag gtaacactat tttcggtcca aatcgcgccg
    12781 ctaatccggg tgcaattggt gcaaacacca tatctcctat tgccataggt aataggtata
    12841 agccagcttt aaaaggagaa agttcttcca caacctgtaa ccattgtgaa gctaataaca
    12901 aaacagatgc cattgcaaac attgtcataa atgcagcaat tgtaccagct gaaaatgatc
    12961 tctttttaaa aagtcttacg tctaacattg gatcagaact tgataaatta cgtttaacaa
    13021 atatcactat catggtaatt gctaatacta ttacaaccca tggaataata tctgctagtc
    13081 cttcttttga aaattctttg atactccata ccagtccaat catgcctgca attgataaaa
    13141 ttgtagaagg aatatcccac gagtgagact tttcttttga taacttagac tctggtaata
    13201 aaaataaacc tgctacaact gctattatcg caaacggtac attaattaaa aatgccgagt
    13261 gccatgaaaa ttgctcaagt aaagctcctc cgataattgg tccaaaaaca gcacctatcg
    13321 atgaagcgat tgaccataca gctaatgcag tggccctttc tttagggttt tcaaaaatta
    13381 ctcttatcat tgaaagagta gttggcatta ttaaagcacc tgcaatacca agtaaaaatc
    13441 gaatagctat tacgaactct gcactttctg cgaaaaatat agctaatgaa acgaggccaa
    13501 ataaagcaaa tccagttaat aatgcttttt ttcttcccca tttatcagca aaggcactca
    13561 atggaattat aaagccagct aaaacaagag agtatatatc aactatccat aactgttggg
    13621 taccagaagg ctctaactct cttactaatt ccggtaaagc cataattaat attgtcatat
    13681 ccattgtaac aacaaacaaa ctaacagcta atactactag tgcagtccat cttttttttg
    13741 atgtcatcat atcagtagtt tttgtaaaaa atgaaatcat ttaagttccc tccaatcctt
    13801 atagaccgtg cgatcggtct ataaggatta taatctattt ttttacatat tacaacaaaa
    13861 aatcttgcga aatttaaatg gctgtattgt gttatacaat tattctttga attttttgtg
    13921 ctatcattga tagtacttaa tactcattaa aggcgtgatg aacttgaaag ataaaatact
    13981 aggtgtcgca aaggaattat ttataaaaaa tggatataat gccactacta ctggagaaat
    14041 tgttaaatta tcagaaagta gtaaagggaa tctttattat cactttaaaa caaaagaaaa
    14101 tctattttta gaaattttaa atatagaaga atctaaatgg caagaacagt ggaaaaagga
    14161 acaaatcaaa tgtaaaacta atagagaaaa attttattta tataatgaac tatctttaac
    14221 aaccgaatac tattatccac ttcaaaacgc aataattgaa ttttacactg aatattataa
    14281 aactaatagc attaatgaaa aaatgaataa attagaaaac aaatatatag atgcttatca
    14341 tgtaattttt aaggagggta atttaaatgg cgaatggtgt attaatgatg ttaatgctgt
    14401 tagtaaaata gcagcaaacg ctgttaatgg aattgttacc tttacacatg aacaaaatat
    14461 taatgaaaga attaaactta tgaacaagtt ctctcaaatt tttttaaatg gacttagtaa
    14521 ataattatat atatattctt gaaataccaa taaatattat ttctaaatta cgttcgttat
    14581 tgagacaaaa tttaatagta ctactacaca aaaagagagt gcgaattcgc actctctttt
    14641 tgtgataaat gaatggttaa gaaaataatt catccacagt tgtatcaaac aatcttgcca
    14701 acttaaatgc taatgtcaat gtaggatcat atttattgtt ttctattgca ttaatagttt
    14761 gcctagacac ttttaattct ttacttaaag tttcttgaga aattcctttt tgttttctat
    14821 attctctaat tctatttttc attaatttaa cctttagatg tatatttttt atttgtagta
    14881 tagtaattaa acagcataag aaatagtgct acaattataa agaaaatttc tggatattga
    14941 gaaaaccaag atccttcaat acttggtgac aaacctgtaa atttaagcac tagtgtaaat
    15001 aacatacaat atacaatcca aataaaactt ttagccagta tttcaataaa cattttacgt
    15061 tgtctttcat cgtatcgatc ttgttgtcct tttttatcta atgtttctga aggtaaatat
    15121 ttgttagtta tagccgtcca taaacaagaa aaaattaaaa taataataaa aggaattaaa
    15181 ttcattataa cacctcataa atgtaaaaag tatttgacat ttatgagtgt aaatattttt
    15241 gatacaaatg tcaaatactt tttacattat aatatatttt aaaacacctt attagtaaaa
    15301 caattactat atagaaagct gcgaactcgt actctttttt cttgtataat tattaaaatc
    15361 tcaattcaat aatatgttat ggaatttgag acactgtgag agacgctcat ttgcattgac
    15421 aatcaattta tcaagataat cccttatcat gtttaattct ataaactgtt tgtcttgtga
    15481 tattaacttc tttcgcaatc ttactaatcg ctttgccttc ttctaacatt tccaccacgc
    15541 gatgataaat aacacgtttc tgtgggtctt tagcatttgg tgaataaagt aaagggcgcc
    15601 ctttatatac gcctttttcc ttcgcaactt gaatcccttg cgcttgtcga cgtttacttt
    15661 catttctctc ttgttctgaa accattgcta atatttgaat gattaaatct ttcataaact
    15721 tatccagtaa tggattgcca attacttcat tcatcatagg taagctggta atcattaact
    15781 gaacgtcttt ttcttttaaa taattcacag tttcaataat ttcatcatag ttacgtccta
    15841 aacgatcaat agattccaca acgaatcgat ctcccattct gacaaaattg agtgcttctt
    15901 gaaatacagg tctattttcc actgactttc ccgactgttt ctctgtaaag attttctcca
    15961 caccaaacgt ctttaaatta tccaattgtc tttctaaatt ttgatctatc gatgatactc
    16021 tcgcataacc tataatcatt catcttcact ccataataaa tatacaccct aatcatacgt
    16081 tttaattgtt ataatttcaa caattctttt tgtatggtta gggtgtgtcc tgtctatttt
    16141 tatctttctt aaaacttagt aatattattt atctttattt tcttgattat ttaacctatt
    16201 gagataacga tagattgtgg tacgtgaaac tttccattgt tctgcaatag ttttaattgg
    16261 tgttccatta tcataaagtg ttttgagtaa ctttaaatct tgtttattta atttttcggg
    16321 acgacctcca tagcgtcctc tagcacgagc ggcaattcgt cctgctgaag aacgttctaa
    16381 aattaagttg cgttcaaact ctgcaaatgc tgcgaataaa tgaaataaca attgtcctgt
    16441 agaacttgat ttatccattg ttatattttc ttctaaacta tgaaaactca cgcctcgatt
    16501 attaagttca ttaactaatg tgattaaatc ttccatatta cgccctagtc tatctagtct
    16561 ccaaacaaca atcgtatctc ctgccctcgc aaattcaatg gctttatcta aaccaggacg
    16621 tttacttttt gatccactta tgtggtcgct aaatatcttt tcacaaccat attgatttaa
    16681 ttgatcttcc tgcaaattta aattttgtaa accagttgaa actcttgcat aacctatttt
    16741 caaaatacaa acctcctata tacatcttca ttgtatcata acttaattct aaccatattt
    16801 atgaacatag attttctgtt gggttattga acactaaaat atgtatactc gctatatata
    16861 tagacataaa aagagagtgt atagaaaatg gttattttct gtacacagtt ttaaatatca
    16921 aaaacattat ttacatttta cgtttaagaa ctaatggtta aaaatttcat tttgtcaaaa
    16981 acgttcgttt ttgagactag aattttatgc cctacttact tcttttattt tcattcaaat
    17041 atttgcttgc atgatgagtc gaaaatggtt ataatacact caaataaata tttgaatgaa
    17101 gatgggatga taatatgaaa aagaaagata cttgtgaaat tttttgttat gacgaagaaa
    17161 aggttaatcg aatacaaggg gatttacaaa cagttgatat ttctggtgtt agccaaattt
    17221 taaaggctat tgccgatgaa aatagagcaa aaattactta cgctctgtgt caggatgaag
    17281 agttgtgtgt ttgtgatata gcaaatatct taggtgttac gatagcaaat gcatctcatc
    17341 atttacgtac gctttataag caaggggtgg tcaactttag aaaagaagga aaactagctt
    17401 tatattcttt aggtgatgag catatcaggc agataatgat gatcgcccta gcacataaga
    17461 aagaagtgaa ggtcaatgtc tgaacaaaag gttaaactaa tggaagaaga aatgaacgtc
    17521 tatcgggtcc aaggatttac atgtgcaaat tgtgcaggaa agtttgagaa aaatgttaaa
    17581 aagattccag gcgttcagga cgcaaa
//
