LOCUS       NZ_CP113033            20267 bp    DNA     linear   CON 14-JAN-2024
DEFINITION  Staphylococcus aureus strain Akali plasmid unnamed1.
ACCESSION   NZ_CP113033
VERSION     NZ_CP113033.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31523637
            Assembly: GCF_026625365.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20267)
  AUTHORS   Huang,Y.-T. and Liu,P.-Y.
  TITLE     Whole genome sequencing of methicillin-resistant Staphylococcus
            aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20267)
  AUTHORS   Huang,Y.-T. and Liu,P.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2022) Computer Science and Information
            Engineering, National Chung Cheng University, No.168, Sec. 1,
            University Rd., Min-Hsiung Township, Chia-yi County 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 306x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 03:23:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,172
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 2,979
            CDSs (with protein)               :: 2,979
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 59 of 113
            Pseudo Genes (incomplete)         :: 51 of 113
            Pseudo Genes (internal stop)      :: 23 of 113
            Pseudo Genes (multiple problems)  :: 19 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..20267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Akali"
                     /isolation_source="environmental"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.23321 N 120.9417 E"
                     /collection_date="2022-06-01"
                     /collected_by="Po-Yu Liu"
     gene            complement(<1..135)
                     /locus_tag="OS080_RS15605"
     CDS             complement(<1..135)
                     /locus_tag="OS080_RS15605"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing protein"
                     /protein_id="WP_267841428.1"
                     /translation="MSEQKVKLMEEEMNVYRVQGFTCANCAGKFEKNVKKIPGVQDAKV
                     "
                     /name="heavy metal-associated domain-containing protein"
     gene            complement(128..496)
                     /gene="cadC"
                     /locus_tag="OS080_RS15610"
     ARG             complement(132..496)
                     /name="cadC"
                     /gene="cadC"
                     /gene_name="Cd(II)/Pb(II)/Zn(II)-sensing metalloregulatory
                     transcriptional repressor CadC"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM/LEAD/ZINC"
                     /identity="100.0"
                     /coverage="100.0"
     gene            868..1446
                     /locus_tag="OS080_RS15615"
     CDS             868..1446
                     /locus_tag="OS080_RS15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690625.1"
                     /translation="MKIGYARVSTGLQNLNLQEDQLNQYGCEKIFSDHISGSKSKRPGL
                     DKAIEFARAGDTIVVWRLDRLGRNMEDLITLVNELNNRGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNGTPI
                     KTIAEQWKVSRTTIYRYLNRLNNQENKDK"
                     /name="recombinase family protein"
     gene            1572..2180
                     /locus_tag="OS080_RS15620"
     CDS             1572..2180
                     /locus_tag="OS080_RS15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            2426..2764
                     /locus_tag="OS080_RS15625"
     CDS             2426..2764
                     /locus_tag="OS080_RS15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            2769..2954
                     /locus_tag="OS080_RS15630"
     CDS             2769..2954
                     /locus_tag="OS080_RS15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(3087..3653)
                     /gene="qacR"
                     /locus_tag="OS080_RS15635"
     ARG             complement(3091..3653)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3831..5375
                     /gene="qac"
                     /locus_tag="OS080_RS15640"
     CDS             3831..5375
                     /gene="qac"
                     /locus_tag="OS080_RS15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_267841560.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGEKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSIMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            5580..6140
                     /locus_tag="OS080_RS15645"
     CDS             5580..6140
                     /locus_tag="OS080_RS15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(6456..7226)
                     /locus_tag="OS080_RS15650"
     CDS             complement(6456..7226)
                     /locus_tag="OS080_RS15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848988.1"
                     /translation="MKTIKMVADELNVTKQTIVNNAKNLNISFKKENGINYINDNDCLK
                     IIEKITKKERTMQNKESIKKERFNENTAEFEKHIYNNSNGFESLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLENQLEEERQLN
                     YSFDTATNDRQSINPQEATFTEEYQNSNQQQKKIQPIEAELNHKGITKAKKDDSSNEDV
                     LKRDDKLSEEIESGPEDREVETPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            7670..8614
                     /locus_tag="OS080_RS15655"
     CDS             7670..8614
                     /locus_tag="OS080_RS15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132442.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSIQNEWVDRNNNIYFIFSNKHLCEYLGYGEQKIIKLKKELVNFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASLTKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTELNNTN
                     YIENEDNDTYDMNDIISNTLINNNHHSNHTNHQKTEFNNDALKFQVLEELPQQLQDYLS
                     KFEIREIRIIKSVLLKGKKSFNTSHDTYYRLEDVEFEIVSVLKRFKAMLLQKNETVEAM
                     QGYLMQSIKAELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            8729..8932
                     /locus_tag="OS080_RS15660"
     CDS             8729..8932
                     /locus_tag="OS080_RS15660"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001796115.1"
                     /translation="MYRKRMLSYGGLLKEKHKILNLDDAEDGNLINTSDEDKTTDEEEK
                     AHSITAIWNFEKQNYYLKNLRS"
                     /name="protein rep"
     gene            8937..9083
                     /locus_tag="OS080_RS15665"
     CDS             8937..9083
                     /locus_tag="OS080_RS15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001092935.1"
                     /translation="MNTKILTGMTGSSLERKINNFINDNQIEVIDIKFSSSVFYFGVMI
                     IYK"
                     /name="hypothetical protein"
     gene            9663..9851
                     /locus_tag="OS080_RS15670"
     CDS             9663..9851
                     /locus_tag="OS080_RS15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001030803.1"
                     /translation="MNKDTMLDKGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            9932..10054
                     /locus_tag="OS080_RS15675"
     CDS             9932..10054
                     /locus_tag="OS080_RS15675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466482.1"
                     /translation="MFFYEDDDFDEMEEKYDYHEDKKIDDAFMPRKFIDVEKNP"
                     /name="hypothetical protein"
     gene            10235..10851
                     /gene="cadD"
                     /locus_tag="OS080_RS15680"
                     /pseudo=""
     CDS             10235..10851
                     /gene="cadD"
                     /locus_tag="OS080_RS15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /name="cadD"
     gene            10870..11217
                     /locus_tag="OS080_RS15685"
     CDS             10870..11217
                     /locus_tag="OS080_RS15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_267841562.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILGKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDGI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            11408..11548
                     /locus_tag="OS080_RS15690"
     CDS             11408..11548
                     /locus_tag="OS080_RS15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001199886.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVRFCCKVK
                     K"
                     /name="hypothetical protein"
     gene            complement(11583..>11684)
                     /locus_tag="OS080_RS15695"
                     /pseudo=""
     CDS             complement(11583..>11684)
                     /locus_tag="OS080_RS15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(12598..13509)
                     /locus_tag="OS080_RS15700"
     CDS             complement(12598..13509)
                     /locus_tag="OS080_RS15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_078056064.1"
                     /translation="MSNFATEPNVSYADILEILKIKKAYNVKQCGNVLEFKPTDEGYLK
                     LHKTWFCKSKLCPVCNWRRAMKNSYQAQKVIEEVIKEKPKARWLFLTLSTKNAIDGDTL
                     EQSLKHLTKAFDRLSRYKKVKQNLVGFMRSTEVTVNKNDGNYNQHMHVLLCVENAYFRK
                     KENYITQEEWVNLWQKALQVDYRPVVNIKAIKPNQKGDKDIESAIKETSKYSVKSSDFL
                     TDDDEKNQEIVSDLEKGLYRKRMLSYGGLLKQKHKILNLDDAEDGNLINTSDEDKITDE
                     EAKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            13546..14220
                     /locus_tag="OS080_RS15705"
     CDS             13546..14220
                     /locus_tag="OS080_RS15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISSau6 family transposase"
                     /protein_id="WP_001105943.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGIYVHHS
                     TIYRWVQEYAPILYQIWKKKNKQAYYKWHIDETYIKIKGQWNYLYRAIDADGHTLDISL
                     RKKRDNHSAYTFIKRLIKQFGKPQMIITNQAPSTKVAMSKVIKDFKLTPNCHCNSKYLN
                     NLIEQDHRHIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRFLQIYGFSPCRVISIML
                     AS"
                     /name="IS6-like element ISSau6 family transposase"
     gene            <14280..14384
                     /locus_tag="OS080_RS15875"
                     /pseudo=""
     CDS             <14280..14384
                     /locus_tag="OS080_RS15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            15891..16499
                     /locus_tag="OS080_RS15710"
     CDS             15891..16499
                     /locus_tag="OS080_RS15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017432088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000793183.1"
                     /translation="MKNLKSFFIKNTLVLTSTALLFSSFEPVVHAAENKEVVKTVENDN
                     VEFNELLEGQEYIWEVLSRDDEGFKMFLDEQRAFNPNFDNDVLLAKQEGALNSSVFANN
                     SMTLNSNKPTPRGPIGATLKAIKALSPSLRHGGTAISWIIKPLSRKHAVLVKKYSRKMS
                     NAIDRLDKSTKSAVENAFVKAGIPRSDAKTLTYIVFLVI"
                     /name="hypothetical protein"
     gene            16977..17072
                     /locus_tag="OS080_RS15715"
     CDS             16977..17072
                     /locus_tag="OS080_RS15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000889979.1"
                     /translation="MLEILVHITTTVISGCIVALFTHWLRNRNDK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            17309..17983
                     /locus_tag="OS080_RS15720"
     CDS             17309..17983
                     /locus_tag="OS080_RS15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105988.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYTLYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(18220..>20267)
                     /gene="cadA"
                     /locus_tag="OS080_RS15725"
     CDS             complement(18220..>20267)
                     /gene="cadA"
                     /locus_tag="OS080_RS15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase CadA"
                     /protein_id="WP_267841564.1"
                     /translation="FGASKIDVYGNASVEELEKAGAFENLKVSPEKLANQTIQRVKDDT
                     KAHKEEKTPFYKKHSTLLFATLLIAFGYLSHFVNGEDNLVTSMLFVGSIVIGGYSLFKV
                     GFQNLIRFDFDMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRS
                     LMDIAPKEALVRRNGQEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITG
                     ESVPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVD
                     KFAKYYTPIIMVIAALVAVVPPLFFGGSWDTWVYQGLAVLVVGCPCALVISTPISIVSA
                     IGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLTKGVPVVTDFEVLNDQVEEKELFSI
                     ITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTSITGRGIKGIVNGTTYYIGSPKL
                     FKELNVSDFSLGFENNVKILQNQGKTAMIIGTEKTILGVIAVADEVRETSKNVILKLHQ
                     LGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELLPQDKLDYIKKMKAEHGNVAMIGDGV
                     NDAPALAASTVGIAMGGAGTDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITF
                     AIGIKIIALLLVIPGWLTLWIAILSDMGATILVALNSLRLMRVKDK"
                     /name="cadA"
     ARG             3832..5372
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.61"
                     /coverage="100.0"
     Typing          9161..9263
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.81"
                     /coverage="100.0"
                     /evalue="1.39e-18"
     Typing          complement(12364..12528)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="5.24e-78"
     Typing          7671..8614
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.577"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8745..8922
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.179"
                     /coverage="95.0"
                     /evalue="3.73e-65"
     Typing          complement(12599..13486)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.625"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacttttgcg tcctgaacgc ctggaatctt tttaacattt ttctcaaact ttcctgcaca
       61 atttgcacat gtaaatcctt ggacccgata gacgttcatt tcttcttcca ttagtttaac
      121 cttttgttca gacattgacc ttcacttctt tcttatgtgc tagggcgatc atcattatct
      181 gcctgatatg ctcatcacct aaagaatata aagctagttt tccttctttt ctaaagttga
      241 ccaccccttg cttataaagc gtacgtaaat gatgagatgc atttgctatc gtaacaccta
      301 agatatttgc tatatcacaa acacacaact cttcatcctg acacagagcg taagtaattt
      361 ttgctctatt ttcatcggca atagccttta aaatttggct aacaccagaa atatcaactg
      421 tttgtaaatc cccttgtatt cgattaacct tttcttcgtc ataacaaaaa atttcacaag
      481 tatctttctt tttcatatta tcatcccatc ttcattcaaa tatttatttg agtgtattat
      541 aaccattttc gactcatcat gcaagcaaat atttgaatga aaataaaaga agtaagtagg
      601 gcataaaatt ctagtctcaa aaacgaacgt ttttgacaaa atgaaatttt taaccattag
      661 ttcttaaacg taaaatgtaa ataatgtttt tgatatttaa aactgtgtac agaaaataac
      721 cattttctat acactctctt tttatgtcta tatatatagc gagtatacat attttagtgt
      781 tcaataaccc aacagaaaat ctatgttcat aaatatggtt agaattaagt tatgatacaa
      841 tgaagatgta tataggaggt ttgtattttg aaaataggtt atgcaagagt ttcaactggt
      901 ttacaaaatt taaatttgca ggaagatcaa ttaaatcaat atggttgtga aaagatattt
      961 agcgaccaca taagtggatc aaaaagtaaa cgtcctggtt tagataaagc cattgaattt
     1021 gcgagggcag gagatacgat tgttgtttgg agactagata gactagggcg taatatggaa
     1081 gatttaatca cattagttaa tgaacttaat aatcgaggcg tgagttttca tagtttagaa
     1141 gaaaatataa caatggataa atcaagttct acaggacaat tgttatttca tttattcgca
     1201 gcatttgcag agtttgaacg caacttaatt ttagaacgtt cttcagcagg acgaattgcc
     1261 gctcgtgcta gaggacgcta tggaggtcgt cccgaaaaat taaataaaca agatttaaag
     1321 ttactcaaaa cactttatga taatggaaca ccaattaaaa ctattgcaga acaatggaaa
     1381 gtttcacgta ccacaatcta tcgttatctc aataggttaa ataatcaaga aaataaagat
     1441 aaataatatt actaagtttt aagaaagata aaaatagaca ggacacaccc taaccataca
     1501 aaaagaattg ttgaaattat aacaattaaa acgtatgatt agggtgtata tttattatgg
     1561 agtgaagatg aatgattata ggttatgcga gagtatcatc gatagatcaa aatttagaaa
     1621 gacaattgga taatttaaag acgtttggtg tggagaaaat ctttacagag aaacagtcgg
     1681 gaaagtcagt ggaaaataga cctgtatttc aagaagcact caattttgtc agaatgggag
     1741 atcgattcgt tgtggaatct attgatcgtt taggacgtaa ctatgatgaa attattgaaa
     1801 ctgtgaatta tttaaaagaa aaagacgttc agttaatgat taccagctta cctatgatga
     1861 atgaagtaat tggcaatcca ttactggata agtttatgaa agatttaatc attcaaatat
     1921 tagcaatggt ttcagaacaa gagagaaatg aaagtaaacg tcgacaagcg caagggattc
     1981 aagttgcgaa ggaaaaaggc gtatataaag ggcgcccttt actttattca ccaaatgcta
     2041 aagacccaca gaaacgtgtt atttatcatc gcgtggtgga aatgttagaa gaaggcaaag
     2101 cgattagtaa gattgcgaaa gaagttaata tcacaagaca aacagtttat agaattaaac
     2161 atgataaggg attatcttga taaattgatt gtcaatgcaa atgagcgtct ctcacagtgt
     2221 ctcaaattcc ataacatatt attgaattga gattttaata attatacaag aaaaaagagt
     2281 acgagttcgc agctttctat atagtaattg ttttactaat aaggtgtttt aaaatatatt
     2341 ataatgtaaa aagtatttga catttgtatc aaaaatattt acactcataa atgtcaaata
     2401 ctttttacat ttatgaggtg ttataatgaa tttaattcct tttattatta ttttaatttt
     2461 ttcttgttta tggacggcta taactaacaa atatttacct tcagaaacat tagataaaaa
     2521 aggacaacaa gatcgatacg atgaaagaca acgtaaaatg tttattgaaa tactggctaa
     2581 aagttttatt tggattgtat attgtatgtt atttacacta gtgcttaaat ttacaggttt
     2641 gtcaccaagt attgaaggat cttggttttc tcaatatcca gaaattttct ttataattgt
     2701 agcactattt cttatgctgt ttaattacta tactacaaat aaaaaatata catctaaagg
     2761 ttaaattaat gaaaaataga attagagaat atagaaaaca aaaaggaatt tctcaagaaa
     2821 ctttaagtaa agaattaaaa gtgtctaggc aaactattaa tgcaatagaa aacaataaat
     2881 atgatcctac attgacatta gcatttaagt tggcaagatt gtttgataca actgtggatg
     2941 aattattttc ttaaccattc atttatcaca aaaagagagt gcgaattcgc actctctttt
     3001 tgtgtagtag tactattaaa ttttgtctca ataacgaacg taatttagaa ataatattta
     3061 ttggtatttc aagaatatat atataattat ttactaagtc catttaaaaa aatttgagag
     3121 aacttgttca taagtttaat tctttcatta atattttgtt catgtgtaaa ggtaacaatt
     3181 ccattaacag cgtttgctgc tattttacta acagcattaa catcattaat acaccattcg
     3241 ccatttaaat taccctcctt aaaaattaca tgataagcat ctatatattt gttttctaat
     3301 ttattcattt tttcattaat gctattagtt ttataatatt cagtgtaaaa ttcaattatt
     3361 gcgttttgaa gtggataata gtattcggtt gttaaagata gttcattata taaataaaat
     3421 ttttctctat tagttttaca tttgatttgt tcctttttcc actgttcttg ccatttagat
     3481 tcttctatat ttaaaatttc taaaaataga ttttcttttg ttttaaagtg ataataaaga
     3541 ttccctttac tactttctga taatttaaca atttctccag tagtagtggc attatatcca
     3601 ttttttataa ataattcctt tgcgacacct agtattttat ctttcaagtt catcacgcct
     3661 ttaatgagta ttaagtacta tcaatgatag cacaaaaaat tcaaagaata attgtataac
     3721 acaatacagc catttaaatt tcgcaagatt ttttgttgta atatgtaaaa aaatagatta
     3781 taatccttat agaccgatcg cacggtctat aaggattgga gggaacttaa atgatttcat
     3841 tttttacaaa aactactgat atgatgacat caaaaaaaag atggactgca ctagtagtat
     3901 tagctgttag tttgtttgtt gttacaatgg atatgacaat attaattatg gctttaccgg
     3961 aattagtaag agagttagag ccttctggta cccaacagtt atggatagtt gatatatact
     4021 ctcttgtttt agctggcttt ataattccat tgagtgcctt tgctgataaa tggggagaaa
     4081 aaaaagcatt attaactgga tttgctttat ttggcctcgt ttcattagct atatttttcg
     4141 cagaaagtgc agagttcgta atagctattc gatttttact tggtattgca ggtgctttaa
     4201 taatgccaac tactctttca atgataagag taatttttga aaaccctaaa gaaagggcca
     4261 ctgcattagc tgtatggtca atcgcttcat cgataggtgc tgtttttgga ccaattatcg
     4321 gaggagcttt acttgagcaa ttttcatggc actcggcatt tttaattaat gtaccgtttg
     4381 cgataatagc agttgtagca ggtttatttt tattaccaga gtctaagtta tcaaaagaaa
     4441 agtctcactc gtgggatatt ccttctacaa ttttatcaat tgcaggcatg attggactgg
     4501 tatggagtat caaagaattt tcaaaagaag gactagcaga tattattcca tgggttgtaa
     4561 tagtattagc aattaccatg atagtgatat ttgttaaacg taatttatca agttctgatc
     4621 caatgttaga cgtaagactt tttaaaaaga gatcattttc agctggtaca attgctgcat
     4681 ttatgacaat gtttgcaatg gcatctgttt tgttattagc ttcacaatgg ttacaggttg
     4741 tggaagaact ttctcctttt aaagctggct tatacctatt acctatggca ataggagata
     4801 tggtgtttgc accaattgca cccggattag cggcgcgatt tggaccgaaa atagtgttac
     4861 cttccggaat tggaattgca gccattggca tgtttattat gtatttcttt ggtcatccat
     4921 tatcatattc tataatggct ttagcattaa ttttagttgg agctggtatg gcttcactag
     4981 cagttgcatc tgctctaata atgttagaaa cacctacatc aaaagcaggt aatgcagctg
     5041 ctgttgaaga gtctatgtat gaccttggaa atgtttttgg tgtagcagta cttggtagcc
     5101 tatcttctat gctttatcgt gtatttttag atatttcatc tttttcatca aaaggtatag
     5161 ttggagattt agctcatgta gctgaagaat ctgtagtggg cgctgtcgaa gtagctaaag
     5221 ctacggggat aaaacagctt gcaaacgagg ctgtaacatc atttaatgat gcttttgtag
     5281 caactgcttt agtaggtggg attatcatga ttatcatttc aatagttgtc tatttgttaa
     5341 ttcccaaatc acttgatata actaaacaaa aatagtacta aacatatatt tttagatatt
     5401 gtagattagt ttatttttaa aattagtaat agttaaaaag tagaaccatt aattttaaat
     5461 ggttctactt ttttattatt aagtaaaatt ataaaagaca atttcttaaa ttaataatat
     5521 gatataccta tatctaataa tttattagta ttaataaacc ttgaaaggat gattaactga
     5581 tgaatagagc agatattttt aaaatatttg atgtagaact taatataact atatttttag
     5641 gagcactagt agcaactttc atctatttta taagttacat gaacaatgta aatgaattaa
     5701 ttacattatg cttaacatta gcttttcctg catatttatt aaaaattagc agatcgttcg
     5761 aggttatttt attagaggaa aaaataaaaa atagtaaaat caaaatctgt gacaaagata
     5821 gaattgaacg agagatactg gtgaaaaaga acctaataga tgaactattg tttcctaata
     5881 gagttattaa aaaatataca ctgacaattt taatttacag tataggtatt attattcttt
     5941 tactactttg taatatgtta agttatggaa ttggtgaaaa tgtcaatttt atctggagat
     6001 tactagcttg ttatgtaacg agtgtcacag tgattatagt tgccttgatt ataatgataa
     6061 tgatatttga tcttataatt ggattactat taaattttca aatattttta aaaaataaaa
     6121 agtatgatga ctttttataa ttattcgatt tatataatga aaaattaaat gattaaacat
     6181 agaaacctca aaacaaacca tatttaatag agtttatggc gccttataca aataaaggct
     6241 atgctgttaa agaattatgt agctatcttc agattaatat ggatgatgtt tatactattg
     6301 gcgatagaaa attgatttac ctatgtttaa taccctagat aattcttctg ataatttagt
     6361 ttttgttaga gtttaacata actgatgatt accagaagtt ctcactatat aaaaggcact
     6421 aaaagttata aacttttagt gcctaattaa ttattttagc taccaaacaa acgactccaa
     6481 aacccctttt taggtgtttc tacttccctg tcctcaggtc cactctctat ttcttcagat
     6541 agtttatcat ctctctttaa tacgtcttcg tttgaactgt catccttttt cgctttagtt
     6601 atgcccttat gatttaattc tgcttctatt ggttgtattt tcttttgttg ttggttgcta
     6661 ttttgatatt cttcagtaaa agttgcttct tgcggattaa tgctttgtct atcattagtc
     6721 gctgtatcaa aagagtaatt aagctgtctt tcttcttcta attggttttc tagtttttga
     6781 atctttttat tactttctaa agctaatatt tgttgattct ccaacagttt atttaatgta
     6841 ttaacattgc tattttgttg atctagttgt ttagttaaat tctcaatata tttttcgtca
     6901 tttttagctc tagtttcaaa gatttctatt tgcttttcta attcatttac ttttgttttt
     6961 aatgactcaa aaccatttga attattgtat atatgctttt caaactctgc agtattttca
     7021 ttaaatcttt ctttttttat tgattcttta ttttgcattg tcctttcttt cttagtgatc
     7081 ttttctataa tttttaaaca atcattatca ttaatataat taattccatt ttctttttta
     7141 aaagatatat ttaagttttt agcattatta acaatagttt gtttagttac attcaattca
     7201 tcagcaacca ttttaatagt tttcataaca ttcatcctta ggtacataaa ttggtaccta
     7261 aattttacca cctaacaaat attttttcat ctttcgacat ctaaatttac tacctatttc
     7321 actactttag tacctaatta gccgttaaaa ccttctatag gtgactaatt aggtactaaa
     7381 gtggtgaatt tttatcattt taaagttaca atgttctttc aactaaactt aaaaagcttt
     7441 atatacaatc ttaaaagtgt taatattact ttgtgaatat taaaagagtc gatactattg
     7501 gcgtagcatc gactctcggt aataaaacga ttcgcattcg tctgtttata taattttttg
     7561 atacttgtat tatatatatc taatcatata agtgcaagta caaaacatat aacctacgta
     7621 aaaattgttt tattacctct atccaaaaag tggaaatgag gtttttatta tgcccaattt
     7681 tgagaaatac aatttatcac aagtaaaaac tgaaagattt taccaacttc ctaaatattt
     7741 gttcgaagat gcatatttta agaaaatgtc agcagaagcc aaaattatgt atgcattatt
     7801 aaaagatcgt ttcgaattat ccatccaaaa tgaatgggta gatagaaata ataacattta
     7861 ctttattttc agtaataaac atttgtgcga gtacttaggt tatggagaac aaaaaatcat
     7921 aaaattgaaa aaagagttag taaattttaa tttattaact caagagcgtg ttggccttaa
     7981 taaaccaaat agattatatt tattaaaacc aaattatgat attgaagcaa gtcttaccaa
     8041 ggaacttcca aattcacagt tccagaacaa tgaatttgga agttctagaa ctgtgaattt
     8101 aagtggtcaa gaacttccaa attcacagtc taatgatact gagttaaata atactaatta
     8161 tatagaaaat gaggataatg atacgtatga tatgaatgat attatatcta atactttaat
     8221 aaataataat catcattcga atcatacaaa tcatcaaaaa actgaattta ataatgacgc
     8281 ccttaagttc caagtactcg aagaattgcc acaacaactt caagactatt taagtaaatt
     8341 tgaaattaga gaaattcgga ttattaaaag cgtattactc aaagggaaaa agtcatttaa
     8401 tacttcacat gatacttatt atcgtttaga agatgttgag tttgaaattg taagtgtatt
     8461 gaaacgtttt aaagcgatgt tgctacaaaa gaatgaaacc gttgaagcta tgcaaggtta
     8521 tttaatgcaa tccattaaag ctgaacttga agaaacacat gccctttata tgcgccgtaa
     8581 aaacatgaaa caatataata tctttaatca ataattcaaa tgattttata acaatcaaag
     8641 agacatcaaa atattccgtt aaatcatctg actttttaac tgatgacgac gaaaaaaatc
     8701 aagaaatttt aagtgatcta gaaaaggttt gtatcgaaaa cgtatgttaa gttatggtgg
     8761 attacttaaa gaaaagcata aaattttaaa cttagacgat gccgaagatg gtaatttgat
     8821 taatacaagt gatgaagata aaacgacaga cgaagaagaa aaagcacatt cgattacagc
     8881 aatttggaat ttcgaaaagc aaaattatta tttaaaaaat ttaaggagtt aaaaatatga
     8941 acactaaaat tttaactgga atgactggaa gttctttaga acgaaaaatc aataatttta
     9001 ttaatgataa ccaaatcgaa gtcattgata ttaagttttc aagttctgta ttttattttg
     9061 gggtaatgat tatttataaa taattaaaga gtttaagaca ttagtgatga ctgatgtctt
     9121 tttttactgt ttttttataa aaaagtactg gcttattttt gtgacaaatg caatatattg
     9181 tgtcacaaaa gtgtgacact taagcttttt atgaccccac ataaaaatat tgttgaagct
     9241 ttgaattgtc tggctttgcc agatacccca tgtatacatg gggtcaaatt tcccttatgc
     9301 tcttacggag ttacggagtt cttagagaaa aaataaaaat tctcgatttt gataaaaaat
     9361 gtcttgcagg aatttagaaa aacatggggg aagttttaaa ggattttatg ctaactttta
     9421 atttgcatgt aaatgggcag tgtttaaaaa aatagtcatt ggttttattt aaaattttgt
     9481 ttttgaaaat agcatatatg tatttggttc atatttgcgt tttaagagat tatgtaggtt
     9541 ttagatagaa aactatatga tttaccccta aatctttaaa ataccccctt aaaattcaaa
     9601 ataaaggtat ttataatttg agtaagtctt gtgataaagt tttttaaaaa ggagttttta
     9661 ttttgaataa agacacaatg ttagataaag gttacgttat agcaactatt ttaaatgttt
     9721 tctttttact tggattaatt tttatatctc atttggaaaa tttatatata ttaattccgt
     9781 atgttatttt gatgggaata aatgctattt atttggttgt taaagttatg aatttcaaaa
     9841 agaataatta gaatatttga ggggattttg aaacgagttt cttcttgttt tcacactatt
     9901 ttttattcct attggtgttg ttgcttactt tttgtttttc tatgaagatg atgattttga
     9961 cgagatggaa gaaaaatatg attatcatga agataaaaaa atagacgacg catttatgcc
    10021 gagaaaattt attgatgttg agaagaaccc ttaactaaac ttgaagacga atgtcggcat
    10081 agcgtgagct attaagccga ccattcgaca agttttggga ttgttagggg ttccgaggct
    10141 caacgtcaat aaagcaattg gaataaagca cctatgatta tttaaaaata ttagttataa
    10201 tacattcaaa aatatttttg aatgaggtgt atgtatgatt caaacggttg tcactgctgc
    10261 tattctttat attgctacag catttgattt attagtaatt ttattaatat tttttgctag
    10321 agcaaagact aaaaaagaat atagagatat ttatattggt caatatttag ggtctattat
    10381 tttaatatta gttagtttgt ttttagcttt tgtattaaat tatgtttcag agaagtggat
    10441 attaggttta ttgggtttaa taccaattta tcttggaatt aaagtggcta tttatgtgat
    10501 tgtgaaggag aaaagagagc taaaaaagaa ctgaatgaaa aaggattgtc taaattagtt
    10561 ggtacggttg caattgttac gatagcaagt tgtggcgccg ataatattgg tttatttgtt
    10621 ccgtattttg tgacattaag tgttactaat ttattactta ctctgtttgt ctttttaatt
    10681 ttaattttct tcttggtgtt tactgcacaa aaattagcta atatcccagg aattggagaa
    10741 attgttgaga aatttagtcg ttggattatg gctattattt atatagcttt aggtttattt
    10801 attattattg aaaatgacac tattcaaaca attttaggat ttatatttta atttagggtg
    10861 tgattttata tgagttatga aaatacttgt gatgtgatct gtgtacatga ggataaagtt
    10921 aacaatgctt taagtttttt agaagatgat aaatctaaga aattacttaa cattttagga
    10981 aaaatttgtg atgagaagaa attgaaaatc atattatctt tgattaaaga agatgagttg
    11041 tgtgtttgtg atatttcttt gatattgaaa atgagtgttg cttcaacttc acatcattta
    11101 aggcttttat ataaaaatga ggtacttgat ttttataaag atggaaagat ggcatattat
    11161 tttattaaag acgatggaat aagagagttt ttctctaaaa atcatgaggg tttttgaaac
    11221 gagtgaaacg agtttcttct tgtcttgata ctatatagaa ataacgttct tttctaaaat
    11281 ccttttgaag cgttgatatg acggcgtttc atactgtttt gcttggggaa aataaaattt
    11341 gacataaaaa aactccagta atatgattaa ggtgtctaat ctaaatcaat actggagttc
    11401 tttttatatg caatataata ctactagaag tataaccgaa aatcaagata ataaaacgtt
    11461 aaaagatatg acgaaaagtg gaaaacaacg cccatggaga gaaaagaaaa tagataatgt
    11521 aaggttctgt tgcaaagtta aaaaataagt gtctaacctt atgtttaaag taatactttg
    11581 tattaactag ctagcatgat gctaattaca cggcatggcg aaaacccgta gatctgaaga
    11641 gatctgcggt tctttttata tagtccataa atacattcaa tgcctgtgta ttttattcca
    11701 attgctttat tgtcgttgag cctcggaacc cttaacaatc ccaaaacttg tcgaatggtc
    11761 ggcttaatag ctcacgctat gccgacattc gtctgcaagt ttagttaagg gttcttctca
    11821 acatcaataa attttctcgg cataaatgcg tcgtctaact ttttttataa tgtttatcaa
    11881 accacgatga aaaaaatata aaaaagactg tataaagaat aaaccaagta tctaaatgat
    11941 tttgattcat ttgcttataa ataaaaaaca ataaaaaaat accaagaata atttgtaaaa
    12001 taaataaaga cctcttcaaa aaaccactcc ttttaaaaaa ctttatctca ggacttattc
    12061 aaattataaa cacctttatt ttgaatttta agagggtatt ttaaagattt agaggtaaat
    12121 cttatagtct tctacttaaa aatctataca agctcttaaa acgcaaatat gagtcaaata
    12181 aatatatggt aatttcacaa acaaaaattt cagcaaaacc agtgactaat tttttaacac
    12241 tgcccattta aatgcaaatt aaaaatgagc ataaaatctt ttaaaacttc cccatgtttt
    12301 tctaaattcc tgcagggcgt ttttatcaaa aatcaagaaa tttcaatttt tctctaagaa
    12361 ctccgtaaga gcataaggag aatttgctat cattcaaaaa taataggtct ggcaaagcca
    12421 gacattccaa gggttttaag taattttaaa gtgatatcat aaaaagctgt agtgtcacac
    12481 ttttgtgaca caatatattg cgtttgtcac agttttgtga cactacatac agcatttgtc
    12541 acaaaaataa gacagtactt ttttacaaaa aatcaataaa aaagacatca gtcatcacta
    12601 atgtcttaaa taataatttt gcttttcaaa attccaaatt gccgtaattg aatgtgcttt
    12661 tgcttcttca tctgttattt tatcttcatc acttgtatta attaaattac catcttcggc
    12721 atcgtctaag tttaaaattt tatgcttttg tttaagcaat ccaccataac ttaacatacg
    12781 ttttcgatat aaaccttttt ctaaatcact tacaatttct tgattttttt cgtcatcgtc
    12841 agttaaaaaa tcagatgact taaccgaata ttttgatgtc tctttgattg ccgattcaat
    12901 atctttatcg cctttttgat taggtttaat tgctttaata tttacaacag gtcgataatc
    12961 aacttgtaat gctttttgcc ataaattaac ccattcttct tgagttatat aattctcttt
    13021 ttttctaaaa tatgcatttt caacacataa caaaacgtgc atatgctgat tataattacc
    13081 gtcattttta ttaacggtaa cttctgttga acgcataaat ccaacaagat tttgtttaac
    13141 ctttttatat ctactcaacc tatcaaatgc tttagttaga tgcttcaaac tttgttctaa
    13201 agtatctcca tctatcgcat tttttgttga aagtgttaaa aacaaccaac gtgcttttgg
    13261 cttttcctta attacttctt caatcacttt ttgagcttga taactatttt tcatagcacg
    13321 cctccaatta caaactggac atagctttga tttacaaaac cacgtcttat gtaatttcaa
    13381 ataaccttca tcggtcggct tgaattctaa gacgttacca cattgtttta cgttataagc
    13441 cttttttatt tttaaaattt caagtatatc tgcatagctt acattaggtt ctgttgcaaa
    13501 gttagaaata tagtataata gtcaacaaaa aatgaggtgc agaggatgaa ttatttcaga
    13561 tataagcaat ttgacaaaga tgtcattact gtagccgttg gctactatct aagatatgca
    13621 ttaagttatc gtgatatatc tgaaatattg agagaacgag gcatctatgt tcatcattca
    13681 acaatttatc gatgggtaca ggaatatgct cctattttgt atcaaatttg gaagaaaaaa
    13741 aataagcaag cttattataa atggcatata gatgagacgt atatcaaaat taaagggcaa
    13801 tggaattatt tatatcgcgc tattgatgca gatggccata cactagatat ttcgttacgt
    13861 aagaaacgag ataatcactc agcatatacg tttattaaac gtcttattaa acaatttggc
    13921 aaacctcaaa tgataattac aaaccaagcc ccttcaacga aggtggcaat gtccaaagtg
    13981 attaaagatt ttaaacttac ccccaactgt cattgtaact ctaaatattt aaataatctc
    14041 attgaacaag atcatcgtca tatcaaagta aggaaaataa gttatcaaag tatcaatacg
    14101 gcaaagaata cgatcaaagg cattgaatgt atttatggac tatataaaaa gaaccgcaga
    14161 tttcttcaga tctacgggtt ttcgccatgc cgtgtaatta gcatcatgct agctagttaa
    14221 tacgaagtat tattttaaac ataaggttag acacttattt tttaactttg caacagaacc
    14281 aagaaatcga tccaactgaa tacctttacc aaatttatta tatttggaat cagaaagagt
    14341 acctggcaag tgaacttcac gaacttaccg aagaagaaca ataaaaaaat ataaaaatat
    14401 tttgagagtt gaccacccac atttgtagtt tacggagaac gtgggtggtc aactctcaaa
    14461 atttatttct taaaaattta tttgttgttc tgatgagcga tgaacgacta aaaaagtata
    14521 atgctatttt ctatcattac acttttttta tacgcttatt atactttcgt tatactcttt
    14581 ttgtcgctac acgaactatg tgatcaaacg gcacgtttgc ccatttaaaa tgcaaattcg
    14641 gaacgattta ttgctaattt ttaaatttgc atgtaaatgg gcagtacgta aaaatcgccc
    14701 gtcctggtgt cgaaaaaacg aacagaaaaa ataaaattaa aatatagtaa tctttttatt
    14761 ttttacaggt aggtttcccc ataatttagt actgcatcag aaatttatct gtacttagaa
    14821 atgacgacgt attttttcta tacgtttatg acatttattc tacattgatc caaatcataa
    14881 aaatgtgttt aaaatgtcct aaaaaggttt taaaaggacg atttttttat attacgtatt
    14941 tttgtactca aaattagaga atgctctaaa tgacctctta aaacgcaaaa aaaatatata
    15001 tcaaaaagac atgtattttt aaaaaaattg atgtgtatga tgcatgtctt ttgtgtatgt
    15061 tttatataca taaagtatat gtatacgtaa cctgtatgta tacataacgt tatggttttt
    15121 atacatatga gttaaaaaag tgatgtcact ttttttctag gaaaaataac tcggcactgc
    15181 cgagttcacc acgttatggg tctgccaaaa tataaaagtg gtggcacttt cccttatgct
    15241 catgacttta tgtataaaag ttagaggtac ttttatgccg agaaaacttt ttgagagatt
    15301 aaattttttt tttttttatt tttttatttt ttacttttat tttcttcaaa tattttgtaa
    15361 atctctttat gagtaaataa aagatagaag cataaagaat aaatgaaaat ttgaaaatat
    15421 aagtttatat gaatgttgta gaaaatcttc aaaattatta agtttaatac gatcatatta
    15481 gcagttataa agaatatgaa taagataata ttattaaatt tctttttgta ttttatatgt
    15541 aaaaatatta taaaaagttc aagaaaaata aatattagat aagttactat aaagaataat
    15601 gcaatatagc caatgttata aaacatatta tttgaatacc aatttattgt tattaaagaa
    15661 cttatattta aattatctgc taatgattgg aaacctatta tgctaaaaaa tggtattgag
    15721 aatactaata cggatatagg tatgattatt attccgagta agaacgttac aattataaag
    15781 ttcaataagt actctttgtt tacttttttc ataataattt aattcctttt atgtatataa
    15841 tatttaataa taaaaaattt taacatttat aattggagga taaaagtcat atgaagaatt
    15901 taaaaagttt ttttattaaa aatactttgg tactaacatc tactgcatta ttattttcta
    15961 gttttgaacc tgtagttcat gctgcagaaa ataaggaagt tgttaaaaca gtagaaaatg
    16021 ataatgtaga attcaatgaa ttgttagaag gacaagaata tatttgggaa gttttatcta
    16081 gagatgatga aggatttaaa atgtttctag atgagcaaag agcttttaat cctaattttg
    16141 ataatgatgt tttactagca aaacaagaag gtgctttaaa ttcttcagta tttgctaata
    16201 attctatgac tttaaactca aataagccta ctcctagagg tcctatcggt gcaactttaa
    16261 aagctattaa ggctctatca ccttctttga gacatggtgg aactgctata tcttggatca
    16321 ttaaaccatt aagtagaaaa catgctgttc tagtaaaaaa atattcaaga aagatgtcta
    16381 atgcaattga taggttagat aagtctacga aatcagctgt tgaaaatgct tttgttaaag
    16441 caggtatacc aagaagtgat gcgaaaactt taacttatat tgtattttta gttatttaga
    16501 tattgagctg acttgcccta agattttata aaatcttagg gcaagttttt ttcttttttc
    16561 ttatcttgat aaagtagtat tttcaatttg tgattggggt gatgattttc tagatacagt
    16621 taaaattaat aatcatagat aatgagagaa cctacaatta tcataagttt tcacctaaaa
    16681 aggatatatt aaagttttac aaatctttaa tgcacctttt agagtgtttt taaatctatt
    16741 ctttaataga ctataatatt cactatttat tacaccgttt tttctttaga ttaatgaatt
    16801 taggtgatga ttttgaactg cgtttcttct tttgtcttga tactataaaa aacgaagtta
    16861 tataacataa gacgacttat cggaagtaga tatgcttgta tattcacaga tggtataata
    16921 atagagtcgc ctatctctca ggcgtcaaat ttagacgcag agaggaggtg ttattcatgc
    16981 tagaaattct tgttcacatc acgaccacag tcatcagtgg ttgtattgtt gcgttattta
    17041 cgcattggct acgcaatcgc aacgataaat aggcgactat agtcacacct aaaaaatccc
    17101 ctcactacgg caatagtgag gggattggtg tattcacgct agaattcttg ttaatcatat
    17161 tataacactg gttagtagag gaatgcaaaa acataagaaa cgtaagtgta actctataat
    17221 tagaattatt aattttcttt gatactataa aagattctgt tgcaaagttg aatttatagt
    17281 ataattttaa caaaaaggag tcttctgtat gaactatttc agatataaac aatttaacaa
    17341 ggatgttatc actgtagccg ttggctacta tctaagatat gcattgagtt atcgtgatat
    17401 atctgaaata ttaagggaac gtggtgtaaa cgttcatcat tcaacggtct accgttgggt
    17461 tcaagaatat gccccaattt tgtatcaaat ttggaagaaa aagcataaaa aagcttatta
    17521 caaatggcgt attgatgaga cgtacatcaa aataaaagga aaatggagct atttatatcg
    17581 tgccattgat gcagagggac atacattaga tatttggttg cgtaagcaac gagataatca
    17641 ttcagcatat gcgtttatca aacgtctcat taaacaattt ggtaaacctc aaaaggtaat
    17701 tacagatcag gcaccttcaa cgaaggtagc aatggctaaa gtaattaaag cttttaaact
    17761 taaacctgac tgtcattgta catcgaaata tctgaataac ctcattgagc aagatcaccg
    17821 tcatattaaa gtaagaaaga caaggtatca aagtatcaat acagcaaaga atactttaaa
    17881 aggtattgaa tgtatttaca ctctatataa aaagaaccgc aggtctcttc agatctacgg
    17941 attttcgcca tgccacgaaa ttagcatcat gctagcaagt taagcgaaca ctgacatgat
    18001 aaattagtgg ttagctatat ttttttactt tgcaacagaa ccagaattaa tataacgaga
    18061 tatcaattcc tgttgctaca tataccgcag ctgctgtaag tatcgttgcg atcatttttc
    18121 catctccaat attttatatt ttaggggtag gacttttctt caaattgaaa agcccccctt
    18181 gaattgcgca cagcgtgacc ttactattct gttttctacc tatttatcct tcactctcat
    18241 cagtcgtaaa ctatttaatg ctaccaaaat agtagctccc atatcggaaa gaatcgctat
    18301 ccaaagggtt aaccatcccg ggataactaa tagtaaggca attattttaa ttccgatagc
    18361 aaaagtgatg ttagctttaa tgatatttaa agtttttcga ctgagtctta ctgcaaatgg
    18421 aagcttactt aaatcatctc ccattaatgc aatatcagct gtctcgatgg cagtatctgt
    18481 tccagcaccg cccattgcaa tgccaacagt ggatgcagca agtgcaggag catcattgac
    18541 gccatcgcca atcatagcta cattaccatg ctcggctttc atttttttaa tatagtccaa
    18601 cttatcctgt ggcaacaatt cggactgaat atcagaaacg cctacatgag caccgattgc
    18661 ttctgcggta ccttgattat cacctgtcag cataattgtt tgcttgattc ctaactgatg
    18721 aagttttaga atcacatttt tacttgtttc gcggacctca tctgcaacgg caattacgcc
    18781 gagaattgtt ttttccgttc caataatcat ggctgttttt ccttggtttt gtaggatttt
    18841 cacattgttt tcaaacccaa ggctaaaatc ggaaacattt aattccttga aaagttttgg
    18901 gcttccaata tagtaagtag ttccgtttac aatccctttt atacctcgcc cagtaatcga
    18961 agtgaattct tccacttgta cattagaata agggatatta tcttgctctg cctttttcat
    19021 tattgctgaa gcaagtggat gttgtgaacg atattctaaa gctgtaatga tagagaatag
    19081 ctctttttct tccacttggt catttaatac ttcaaaatct gttaccactg gtacaccttt
    19141 tgtcagtgtt cctgttttat caaatgcgat tgccttaatg gctcctaatt cttctagata
    19201 gacaccgcct ttaatcaaca cacctttttt agctgcattt ccaattgccg agacaatcga
    19261 gattggagta gaaataacta atgcacacgg acatccaact acaagtaccg ctaatccttg
    19321 ataaacccaa gtatcccaac ttccaccaaa gaataaaggt ggaacgactg caacgagcgc
    19381 cgcaataacc ataatgatcg gcgtataata tttcgcaaat ttatctacga atgcttgcgc
    19441 tggagcgcgc tccccttgtg cttcctcaac cagatgaata atcttggaga tagttgtatc
    19501 ctctacgtat ttggtgattt ttacttcaag tagtccctct tcgttaagcg tacctgcaaa
    19561 tacttcatca tctaccgttt tggcaacagg gacagattct cctgttatag cagcctggtt
    19621 gacagccgac acaccattta taatgatccc atccatggca attttctccc ctggtttgac
    19681 gatcataata tcacccacgg caatatcgtc cacatggatc attatttcct gaccattccg
    19741 cctaacaagt gcttcttttg gggcaatatc catcaatgaa cgaatggact gtcttgctct
    19801 atccatagaa aaacgttcaa gtgcttcact gattgcaaag agaacgacaa caatggatgc
    19861 ctctgcccat tcaccaatga tggcagctcc aataactgct acggtcatca gggttttcat
    19921 gtcgaaatca aagcgtatca aattttgaaa accaacttta aatagtgaat atccgccaat
    19981 tacaatcgaa cctacaaata acatggaagt tacgaggtta tcttctccat ttacaaagtg
    20041 agaaaggtaa ccaaaagcaa ttagtagtgt ggcaaacagc aatgtactat gttttttata
    20101 aaatggtgtt ttctcttctt tatgagcctt agtgtcatct ttaacccttt gtatcgtttg
    20161 attcgctagt ttttcaggag atacttttag attctcaaaa gcacctgctt tttcaagttc
    20221 ttcaaccgat gcatttccat atacatcaat tttagaagcg ccaaagt
//
