LOCUS       NZ_CP113011            11358 bp    DNA     linear   CON 14-JAN-2024
DEFINITION  Staphylococcus aureus strain Zed plasmid unnamed1.
ACCESSION   NZ_CP113011
VERSION     NZ_CP113011.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31523632
            Assembly: GCF_026625265.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Huang,Y.-T. and Liu,P.-Y.
  TITLE     Whole genome sequencing of methicillin-resistant Staphylococcus
            aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11358)
  AUTHORS   Huang,Y.-T. and Liu,P.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2022) Computer Science and Information
            Engineering, National Chung Cheng University, No.168, Sec. 1,
            University Rd., Min-Hsiung Township, Chia-yi County 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:53:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,154
            CDSs (total)                      :: 3,074
            Genes (coding)                    :: 2,965
            CDSs (with protein)               :: 2,965
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 62 of 109
            Pseudo Genes (incomplete)         :: 47 of 109
            Pseudo Genes (internal stop)      :: 21 of 109
            Pseudo Genes (multiple problems)  :: 20 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Zed"
                     /isolation_source="environmental"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.23321 N 120.9417 E"
                     /collection_date="2022-06-01"
                     /collected_by="Po-Yu Liu"
     gene            <1..483
                     /locus_tag="OS078_RS00005"
     CDS             <1..483
                     /locus_tag="OS078_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISSau6 family transposase"
                     /protein_id="WP_267836443.1"
                     /translation="KNKQAYYKWHIDETYIKIKGQWNYLYRAIDADGHTLDISLRKKRD
                     NHSAYTFIKRLIKQFGKPQMIITNQAPSTKVAMSKVIKDFKLTPNCHCNSKYLNNLIEQ
                     DHRHIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRFLQIYGFSPCRVISIMLAS"
                     /name="IS6-like element ISSau6 family transposase"
     gene            <543..647
                     /locus_tag="OS078_RS15770"
                     /pseudo=""
     CDS             <543..647
                     /locus_tag="OS078_RS15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            2155..2763
                     /locus_tag="OS078_RS00010"
     CDS             2155..2763
                     /locus_tag="OS078_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017432088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000793183.1"
                     /translation="MKNLKSFFIKNTLVLTSTALLFSSFEPVVHAAENKEVVKTVENDN
                     VEFNELLEGQEYIWEVLSRDDEGFKMFLDEQRAFNPNFDNDVLLAKQEGALNSSVFANN
                     SMTLNSNKPTPRGPIGATLKAIKALSPSLRHGGTAISWIIKPLSRKHAVLVKKYSRKMS
                     NAIDRLDKSTKSAVENAFVKAGIPRSDAKTLTYIVFLVI"
                     /name="hypothetical protein"
     gene            3241..3336
                     /locus_tag="OS078_RS00015"
     CDS             3241..3336
                     /locus_tag="OS078_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000889979.1"
                     /translation="MLEILVHITTTVISGCIVALFTHWLRNRNDK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3573..4247
                     /locus_tag="OS078_RS00020"
     CDS             3573..4247
                     /locus_tag="OS078_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105988.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYTLYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(4482..6665)
                     /gene="cadA"
                     /locus_tag="OS078_RS00025"
     CDS             complement(4482..6665)
                     /gene="cadA"
                     /locus_tag="OS078_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase CadA"
                     /protein_id="WP_000003264.1"
                     /translation="MSEQKVKLMEEEMNVYRVQGFTCANCAGKFEKNVKKIPGVQDAKV
                     NFGASKIDVYGNASVEELEKAGAFENLKVSPEKLANQTIQRVKDDTKAHKEEKTPFYKK
                     HSTLLFATLLIAFGYLSHFVNGEDNLVTSMLFVGSIVIGGYSLFKVGFQNLIRFDFDMK
                     TLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALVMR
                     NGQEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGESVPVAKTVDDEV
                     FAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFAKYYTPIIMVI
                     AALVAVVPPLFFGGSWDTWVYQGLAVLVVGCPCALVISTPISIVSAIGNAAKKGVLIKG
                     GVYLEELGAIKAIAFDKTGTLTKGVPVVTDFEVLNDQVEEKELFSIITALEYRSQHPLA
                     SAIMKKAEQDNIPYSNVQVEEFTSITGRGIKGIVNGTTYYIGSPKLFKELNVSDFSLGF
                     ENNVKILQNQGKTAMIIGTEKTILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDN
                     QGTANAIGTHVGVSDIQSELMPQDKLDYIKKMQSEYDNVAMIGDGVNDAPALAASTVGI
                     AMGGAGTDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVI
                     PGWLTLWIAILSDMGATILVALNSLRLMRVKDK"
                     /name="cadA"
     gene            complement(6658..7026)
                     /gene="cadC"
                     /locus_tag="OS078_RS00030"
     ARG             complement(6662..7026)
                     /name="cadC"
                     /gene="cadC"
                     /gene_name="Cd(II)/Pb(II)/Zn(II)-sensing metalloregulatory
                     transcriptional repressor CadC"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM/LEAD/ZINC"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7398..7976
                     /locus_tag="OS078_RS00035"
     CDS             7398..7976
                     /locus_tag="OS078_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690625.1"
                     /translation="MKIGYARVSTGLQNLNLQEDQLNQYGCEKIFSDHISGSKSKRPGL
                     DKAIEFARAGDTIVVWRLDRLGRNMEDLITLVNELNNRGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNGTPI
                     KTIAEQWKVSRTTIYRYLNRLNNQENKDK"
                     /name="recombinase family protein"
     gene            8102..8710
                     /locus_tag="OS078_RS00040"
     CDS             8102..8710
                     /locus_tag="OS078_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            8956..9294
                     /locus_tag="OS078_RS00045"
     CDS             8956..9294
                     /locus_tag="OS078_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            9299..9484
                     /locus_tag="OS078_RS00050"
     CDS             9299..9484
                     /locus_tag="OS078_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(9617..10183)
                     /gene="qacR"
                     /locus_tag="OS078_RS00055"
     ARG             complement(9621..10183)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            10361..>11358
                     /locus_tag="OS078_RS00060"
     CDS             10361..>11358
                     /locus_tag="OS078_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_267836444.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPG"
                     /name="MFS transporter"
     ARG             10362..11356
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.7"
                     /coverage="64.59"
ORIGIN
        1 aaaaataagc aagcttatta taaatggcat atagatgaga cgtatatcaa aattaaaggg
       61 caatggaatt atttatatcg cgctattgat gcagatggcc atacactaga tatttcgtta
      121 cgtaagaaac gagataatca ctcagcatat acgtttatta aacgtcttat taaacaattt
      181 ggcaaacctc aaatgataat tacaaaccaa gccccttcaa cgaaggtggc aatgtccaaa
      241 gtgattaaag attttaaact tacccccaac tgtcattgta actctaaata tttaaataat
      301 ctcattgaac aagatcatcg tcatatcaaa gtaaggaaaa taagttatca aagtatcaat
      361 acggcaaaga atacgatcaa aggcattgaa tgtatttatg gactatataa aaagaaccgc
      421 agatttcttc agatctacgg gttttcgcca tgccgtgtaa ttagcatcat gctagctagt
      481 taatacgaag tattatttta aacataaggt tagacactta ttttttaact ttgcaacaga
      541 accaagaaat cgatccaact gaataccttt accaaattta ttatatttgg aatcagaaag
      601 agtacctggc aagtgaactt cacgaactta ccgaagaaga acaataaaaa aatataaaaa
      661 tattttgaga gttgaccacc cacatttgta gtttacggag aacgtgggtg gtcaactctc
      721 aaaatttatt tcttaaaaat ttatttgttg ttctgatgag cgatgaacga ctaaaaaagt
      781 ataatgctat tttctatcat tacacttttt ttatacgctt attatacttt cgttatactc
      841 tttttgtcgc tacacgaact atgtgatcaa acggcacgtt tgcccattta aaatgcaaat
      901 tcggaacgat ttattgctaa tttttaaatt tgcatgtaaa tgggcagtac gtaaaaatcg
      961 cccgtcctgg tgtcgaaaaa acgaacagaa aaaataaaat taaaatatag taatcttttt
     1021 attttttaca ggtaggtttc cccataattt agtactgcat cagaaattta tctgtactta
     1081 gaaatgacga cgtatttttt ctatacgttt atgacattta ttctacattg atccaaatca
     1141 taaaaatgtg tttaaaatgt cctaaaaagg ttttaaaagg acgatttttt tatattacgt
     1201 atttttgtac tcaaaattag agaatgctct aaatgacctc ttaaaacgca aaaaaaatat
     1261 atatcaaaaa gacatgtatt tttaaaaaaa ttgatgtgta tgatgcatgt cttttgtgta
     1321 tgttttatat acataaagta tatgtatacg taacctgtat gtatacataa cgttatggtt
     1381 tttatacata tgagttaaaa aagtgatgtc actttttttc taggaaaaat aactcggcac
     1441 tgccgagttc accacgttat gggtctgcca aaatataaaa gtggtggcac tttcccttat
     1501 gctcatgact ttatgtataa aagttagagg tacttttatg ccgagaaaac tttttgagag
     1561 attaattttt ttattttttt tattttttta ttttttactt ttattttctt caaatatttt
     1621 gtaaatctct ttatgagtaa ataaaagata gaagcataaa gaataaatga aaatttgaaa
     1681 atataagttt atatgaatgt tgtagaaaat cttcaaaatt attaagttta atacgatcat
     1741 attagcagtt ataaagaata tgaataagat aatattatta aatttctttt tgtattttat
     1801 atgtaaaaat attataaaaa gttcaagaaa aataaatatt agataagtta ctataaagaa
     1861 taatgcaata tagccaatgt tataaaacat attatttgaa taccaattta ttgttattaa
     1921 agaacttata tttaaattat ctgctaatga ttggaaacct attatgctaa aaaatggtat
     1981 tgagaatact aatacggata taggtatgat tattattccg agtaagaacg ttacaattat
     2041 aaagttcaat aagtactctt tgtttacttt tttcataata atttaattcc ttttatgtat
     2101 ataatattta ataataaaaa attttaacat ttataattgg aggataaaag tcatatgaag
     2161 aatttaaaaa gtttttttat taaaaatact ttggtactaa catctactgc attattattt
     2221 tctagttttg aacctgtagt tcatgctgca gaaaataagg aagttgttaa aacagtagaa
     2281 aatgataatg tagaattcaa tgaattgtta gaaggacaag aatatatttg ggaagtttta
     2341 tctagagatg atgaaggatt taaaatgttt ctagatgagc aaagagcttt taatcctaat
     2401 tttgataatg atgttttact agcaaaacaa gaaggtgctt taaattcttc agtatttgct
     2461 aataattcta tgactttaaa ctcaaataag cctactccta gaggtcctat cggtgcaact
     2521 ttaaaagcta ttaaggctct atcaccttct ttgagacatg gtggaactgc tatatcttgg
     2581 atcattaaac cattaagtag aaaacatgct gttctagtaa aaaaatattc aagaaagatg
     2641 tctaatgcaa ttgataggtt agataagtct acgaaatcag ctgttgaaaa tgcttttgtt
     2701 aaagcaggta taccaagaag tgatgcgaaa actttaactt atattgtatt tttagttatt
     2761 tagatattga gctgacttgc cctaagattt tataaaatct tagggcaagt ttttttcttt
     2821 tttcttatct tgataaagta gtattttcaa tttgtgattg gggtgatgat tttctagata
     2881 cagttaaaat taataatcat agataatgag agaacctaca attatcataa gttttcacct
     2941 aaaaaggata tattaaagtt ttacaaatct ttaatgcacc ttttagagtg tttttaaatc
     3001 tattctttaa tagactataa tattcactat ttattacacc gttttttctt tagattaatg
     3061 aatttaggtg atgattttga actgcgtttc ttcttttgtc ttgatactat aaaaaacgaa
     3121 gttatataac ataagacgac ttatcggaag tagatatgct tgtatattca cagatggtat
     3181 aataatagag tcgcctatct ctcaggcgtc aaatttagac gcagagagga ggtgttattc
     3241 atgctagaaa ttcttgttca catcacgacc acagtcatca gtggttgtat tgttgcgtta
     3301 tttacgcatt ggctacgcaa tcgcaacgat aaataggcga ctatagtcac acctaaaaaa
     3361 tcccctcact acggcaatag tgaggggatt ggtgtattca cgctagaatt cttgttaatc
     3421 atattataac actggttagt agaggaatgc aaaaacataa gaaacgtaag tgtaactcta
     3481 taattagaat tattaatttt ctttgatact ataaaagatt ctgttgcaaa gttgaattta
     3541 tagtataatt ttaacaaaaa ggagtcttct gtatgaacta tttcagatat aaacaattta
     3601 acaaggatgt tatcactgta gccgttggct actatctaag atatgcattg agttatcgtg
     3661 atatatctga aatattaagg gaacgtggtg taaacgttca tcattcaacg gtctaccgtt
     3721 gggttcaaga atatgcccca attttgtatc aaatttggaa gaaaaagcat aaaaaagctt
     3781 attacaaatg gcgtattgat gagacgtaca tcaaaataaa aggaaaatgg agctatttat
     3841 atcgtgccat tgatgcagag ggacatacat tagatatttg gttgcgtaag caacgagata
     3901 atcattcagc atatgcgttt atcaaacgtc tcattaaaca atttggtaaa cctcaaaagg
     3961 taattacaga tcaggcacct tcaacgaagg tagcaatggc taaagtaatt aaagctttta
     4021 aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt gagcaagatc
     4081 accgtcatat taaagtaaga aagacaaggt atcaaagtat caatacagca aagaatactt
     4141 taaaaggtat tgaatgtatt tacactctat ataaaaagaa ccgcaggtct cttcagatct
     4201 acggattttc gccatgccac gaaattagca tcatgctagc aagttaagcg aacactgaca
     4261 tgataaatta gtggttagct atatttttac tttgcaacag aaccagaatt aatataacga
     4321 gatatcaatt cctgttgcta catataccgc agctgctgta agtatcgttg cgatcatttt
     4381 tccatctcca atattttata ttttaggggt aggacttttc ttcaaattga aaagcccccc
     4441 ttgaattgcg cacagcgtga ccttactatt ctgttttcta cctatttatc cttcactctc
     4501 atcagtcgta aactatttaa tgctaccaaa atagtagctc ccatatcgga aagaatcgct
     4561 atccaaaggg ttaaccatcc cgggataact aatagtaagg caattatttt aattccgata
     4621 gcaaaagtga tgttagcttt aatgatattt aaagtttttc gactgagtct tactgcaaat
     4681 ggaagcttac ttaaatcatc tcccattaat gcaatatcag ctgtttcaat tgcagtatcc
     4741 gttccagcac cgcccattgc aattccaaca gtagatgcag caagtgctgg agcatcatta
     4801 acgccatcgc caatcatagc tacattatca tactccgatt gcattttttt aatataatct
     4861 aatttatcct gtggcatcaa ttcagactga atatcagaaa cgcctacatg tgtaccgatt
     4921 gcatttgcag taccttgatt atcacctgtc agcataattg tttgcttgat acctaactga
     4981 tgaagttttt gaatcacatt tttacttgtt tcacgaacct catctgcaac ggcaattacg
     5041 ccgagaattg ttttttccgt tccaataatc atggctgttt ttccttggtt ttgtaggatt
     5101 ttcacattgt tttcaaaccc aaggctaaaa tcggaaacat ttaattcctt gaaaagtttt
     5161 gggcttccaa tatagtaagt agttccgttt acaatccctt ttatacctcg cccagtaatc
     5221 gaagtgaatt cttccacttg tacattagaa taagggatat tatcttgctc tgcctttttc
     5281 attattgctg aagcaagtgg atgttgtgaa cgatattcta aagctgtaat gatagagaat
     5341 agctcttttt cttccacttg gtcatttaat acttcaaaat ctgttaccac tggtacacct
     5401 tttgtcagtg ttcctgtttt atcaaatgcg attgccttaa tggctcctaa ttcttctaga
     5461 tagacaccgc ctttaatcaa cacacctttt ttagctgcat ttccaattgc cgagacaatc
     5521 gagattggag tagaaataac taatgcacac ggacatccaa ctacaagtac cgctaatcct
     5581 tgataaaccc aagtatccca acttccacca aagaataaag gtggaacgac tgcaacgagc
     5641 gccgcaataa ccataatgat cggcgtataa tatttcgcaa atttatctac gaatgcttgc
     5701 gctggagcgc gctccccttg tgcttcctca accagatgaa taatcttgga gatagttgta
     5761 tcctctacgt atttggtgat ttttacttca agtagtccct cttcgttaag cgtacctgca
     5821 aatacttcat catctaccgt tttggcaaca gggacagatt ctcctgttat agcagcctgg
     5881 ttgacagccg acacaccatt tataatgatc ccatccatgg caattttctc ccctggtttg
     5941 acgatcataa tatcacccac ggcaatatcg tccacatgga tcattatttc ctgaccattc
     6001 cgcataacaa gtgcttcttt tggggcaata tccatcaatg aacgaatgga ctgtcttgct
     6061 ctatccatag aaaaacgttc aagtgcttca ctgattgcaa agagaacgac aacaatggat
     6121 gcctctgccc attcaccaat gatggcagct ccaataactg ctacggtcat cagggttttc
     6181 atgtcgaaat caaagcgtat caaattttga aaaccaactt taaatagtga atatccgcca
     6241 attacaatcg aacctacaaa taacatggaa gttacgaggt tatcttctcc atttacaaag
     6301 tgagaaaggt aaccaaaagc aattagtagt gtggcaaaca gcaatgtact atgtttttta
     6361 taaaatggtg ttttctcttc tttatgagcc ttagtgtcat ctttaaccct ttgtatcgtt
     6421 tgattcgcta gtttttcagg agatactttt agattctcaa aagcacctgc tttttcaagt
     6481 tcttcaaccg atgcatttcc atatacatca attttagaag cgccaaagtt tacttttgcg
     6541 tcctgaacgc ctggaatctt tttaacattt ttctcaaact ttcctgcaca atttgcacat
     6601 gtaaatcctt ggacccgata gacgttcatt tcttcttcca ttagtttaac cttttgttca
     6661 gacattgacc ttcacttctt tcttatgtgc tagggcgatc atcattatct gcctgatatg
     6721 ctcatcacct aaagaatata aagctagttt tccttctttt ctaaagttga ccaccccttg
     6781 cttataaagc gtacgtaaat gatgagatgc atttgctatc gtaacaccta agatatttgc
     6841 tatatcacaa acacacaact cttcatcctg acacagagcg taagtaattt ttgctctatt
     6901 ttcatcggca atagccttta aaatttggct aacaccagaa atatcaactg tttgtaaatc
     6961 cccttgtatt cgattaacct tttcttcgtc ataacaaaaa atttcacaag tatctttctt
     7021 tttcatatta tcatcccatc ttcattcaaa tatttatttg agtgtattat aaccattttc
     7081 gactcatcat gcaagcaaat atttgaatga aaataaaaga agtaagtagg gcataaaatt
     7141 ctagtctcaa aaacgaacgt ttttgacaaa atgaaatttt taaccattag ttcttaaacg
     7201 taaaatgtaa ataatgtttt tgatatttaa aactgtgtac agaaaataac cattttctat
     7261 acactctctt tttatgtcta tatatatagc gagtatacat attttagtgt tcaataaccc
     7321 aacagaaaat ctatgttcat aaatatggtt agaattaagt tatgatacaa tgaagatgta
     7381 tataggaggt ttgtattttg aaaataggtt atgcaagagt ttcaactggt ttacaaaatt
     7441 taaatttgca ggaagatcaa ttaaatcaat atggttgtga aaagatattt agcgaccaca
     7501 taagtggatc aaaaagtaaa cgtcctggtt tagataaagc cattgaattt gcgagggcag
     7561 gagatacgat tgttgtttgg agactagata gactagggcg taatatggaa gatttaatca
     7621 cattagttaa tgaacttaat aatcgaggcg tgagttttca tagtttagaa gaaaatataa
     7681 caatggataa atcaagttct acaggacaat tgttatttca tttattcgca gcatttgcag
     7741 agtttgaacg caacttaatt ttagaacgtt cttcagcagg acgaattgcc gctcgtgcta
     7801 gaggacgcta tggaggtcgt cccgaaaaat taaataaaca agatttaaag ttactcaaaa
     7861 cactttatga taatggaaca ccaattaaaa ctattgcaga acaatggaaa gtttcacgta
     7921 ccacaatcta tcgttatctc aataggttaa ataatcaaga aaataaagat aaataatatt
     7981 actaagtttt aagaaagata aaaatagaca ggacacaccc taaccataca aaaagaattg
     8041 ttgaaattat aacaattaaa acgtatgatt agggtgtata tttattatgg agtgaagatg
     8101 aatgattata ggttatgcga gagtatcatc gatagatcaa aatttagaaa gacaattgga
     8161 taatttaaag acgtttggtg tggagaaaat ctttacagag aaacagtcgg gaaagtcagt
     8221 ggaaaataga cctgtatttc aagaagcact caattttgtc agaatgggag atcgattcgt
     8281 tgtggaatct attgatcgtt taggacgtaa ctatgatgaa attattgaaa ctgtgaatta
     8341 tttaaaagaa aaagacgttc agttaatgat taccagctta cctatgatga atgaagtaat
     8401 tggcaatcca ttactggata agtttatgaa agatttaatc attcaaatat tagcaatggt
     8461 ttcagaacaa gagagaaatg aaagtaaacg tcgacaagcg caagggattc aagttgcgaa
     8521 ggaaaaaggc gtatataaag ggcgcccttt actttattca ccaaatgcta aagacccaca
     8581 gaaacgtgtt atttatcatc gcgtggtgga aatgttagaa gaaggcaaag cgattagtaa
     8641 gattgcgaaa gaagttaata tcacaagaca aacagtttat agaattaaac atgataaggg
     8701 attatcttga taaattgatt gtcaatgcaa atgagcgtct ctcacagtgt ctcaaattcc
     8761 ataacatatt attgaattga gattttaata attatacaag aaaaaagagt acgagttcgc
     8821 agctttctat atagtaattg ttttactaat aaggtgtttt aaaatatatt ataatgtaaa
     8881 aagtatttga catttgtatc aaaaatattt acactcataa atgtcaaata ctttttacat
     8941 ttatgaggtg ttataatgaa tttaattcct tttattatta ttttaatttt ttcttgttta
     9001 tggacggcta taactaacaa atatttacct tcagaaacat tagataaaaa aggacaacaa
     9061 gatcgatacg atgaaagaca acgtaaaatg tttattgaaa tactggctaa aagttttatt
     9121 tggattgtat attgtatgtt atttacacta gtgcttaaat ttacaggttt gtcaccaagt
     9181 attgaaggat cttggttttc tcaatatcca gaaattttct ttataattgt agcactattt
     9241 cttatgctgt ttaattacta tactacaaat aaaaaatata catctaaagg ttaaattaat
     9301 gaaaaataga attagagaat atagaaaaca aaaaggaatt tctcaagaaa ctttaagtaa
     9361 agaattaaaa gtgtctaggc aaactattaa tgcaatagaa aacaataaat atgatcctac
     9421 attgacatta gcatttaagt tggcaagatt gtttgataca actgtggatg aattattttc
     9481 ttaaccattc atttatcaca aaaagagagt gcgaattcgc actctctttt tgtgtagtag
     9541 tactattaaa ttttgtctca ataacgaacg taatttagaa ataatattta ttggtatttc
     9601 aagaatatat atataattat ttactaagtc catttaaaaa aatttgagag aacttgttca
     9661 taagtttaat tctttcatta atattttgtt catgtgtaaa ggtaacaatt ccattaacag
     9721 cgtttgctgc tattttacta acagcattaa catcattaat acaccattcg ccatttaaat
     9781 taccctcctt aaaaattaca tgataagcat ctatatattt gttttctaat ttattcattt
     9841 tttcattaat gctattagtt ttataatatt cagtgtaaaa ttcaattatt gcgttttgaa
     9901 gtggataata gtattcggtt gttaaagata gttcattata taaataaaat ttttctctat
     9961 tagttttaca tttgatttgt tcctttttcc actgttcttg ccatttagat tcttctatat
    10021 ttaaaatttc taaaaataga ttttcttttg ttttaaagtg ataataaaga ttccctttac
    10081 tactttctga taatttaaca atttctccag tagtagtggc attatatcca ttttttataa
    10141 ataattcctt tgcgacacct agtattttat ctttcaagtt catcacgcct ttaatgagta
    10201 ttaagtacta tcaatgatag cacaaaaaat tcaaagaata attgtataac acaatacagc
    10261 catttaaatt tcgcaagatt ttttgttgta atatgtaaaa aaatagatta taatccttat
    10321 agaccgatcg cacggtctat aaggattgga gggaacttaa atgatttcat tttttacaaa
    10381 aactactgat atgatgacat caaaaaaaag atggactgca ctagtagtat tagctgttag
    10441 tttgtttgtt gttacaatgg atatgacaat attaattatg gctttaccgg aattagtaag
    10501 agagttagag ccttctggta cccaacagtt atggatagtt gatatatact ctcttgtttt
    10561 agctggcttt ataattccat tgagtgcctt tgctgataaa tggggaagaa aaaaagcatt
    10621 attaactgga tttgctttat ttggcctcgt ttcattagct atatttttcg cagaaagtgc
    10681 agagttcgta atagctattc gatttttact tggtattgca ggtgctttaa taatgccaac
    10741 tactctttca atgataagag taatttttga aaaccctaaa gaaagggcca ctgcattagc
    10801 tgtatggtca atcgcttcat cgataggtgc tgtttttgga ccaattatcg gaggagcttt
    10861 acttgagcaa ttttcatggc actcggcatt tttaattaat gtaccgtttg cgataatagc
    10921 agttgtagca ggtttatttt tattaccaga gtctaagtta tcaaaagaaa agtctcactc
    10981 gtgggatatt ccttctacaa ttttatcaat tgcaggcatg attggactgg tatggagtat
    11041 caaagaattt tcaaaagaag gactagcaga tattattcca tgggttgtaa tagtattagc
    11101 aattaccatg atagtgatat ttgttaaacg taatttatca agttctgatc caatgttaga
    11161 cgtaagactt tttaaaaaga gatcattttc agctggtaca attgctgcat ttatgacaat
    11221 gtttgcaatg gcatctgttt tgttattagc ttcacaatgg ttacaggttg tggaagaact
    11281 ttctcctttt aaagctggct tatacctatt acctatggca ataggagata tggtgtttgc
    11341 accaattgca cccggatt
//
