LOCUS       NZ_CP112889             4232 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella electrica strain S2-IND-01-C plasmid p2-S2-IND-01-C,
            complete sequence.
ACCESSION   NZ_CP112889
VERSION     NZ_CP112889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31765424
            Assembly: GCF_027921545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella electrica
  ORGANISM  Klebsiella electrica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Aldeia,C., Campos-Madueno,E.I., Sendi,P. and Endimiani,A.
  TITLE     Complete Genome Sequence of the First Colistin-Resistant Raoultella
            electrica Strain
  JOURNAL   Microbiol Resour Announc 12 (5), e0004723 (2023)
   PUBMED   37014211
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Aldeia,C., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2022) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3010, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 425.089x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 13:14:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,391
            CDSs (total)                      :: 5,272
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 53 of 135
            Pseudo Genes (incomplete)         :: 112 of 135
            Pseudo Genes (internal stop)      :: 18 of 135
            Pseudo Genes (multiple problems)  :: 40 of 135
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP112889.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Klebsiella electrica"
                     /mol_type="genomic DNA"
                     /strain="S2-IND-01-C"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1259973"
                     /plasmid="p2-S2-IND-01-C"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2022"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(3..614)
                     /locus_tag="OR613_RS26870"
                     /old_locus_tag="OR613_26865"
     CDS             complement(3..614)
                     /locus_tag="OR613_RS26870"
                     /old_locus_tag="OR613_26865"
                     /inference="COORDINATES: protein motif:HMM:NF025107.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184132.1"
                     /translation="MSTENAQKEQKKESLITTHWGEDATLHGWTAVPNSLLMLQGDLGI
                     GSTEMCILLNVLMHQWPESGESISFPSIGTIASRMGVSKRTIQRGVSNLESLGILTRHQ
                     STRNDPRTNGANIFDSTPLKEHLNKKSKGITISRNKKKERKNIGTISNIKLPRLCPKCL
                     KTKATSYEEIVSFFGIRKTMAGEVINSYCKECRASEKNIF"
                     /name="helix-turn-helix domain-containing protein"
     gene            933..1658
                     /locus_tag="OR613_RS26875"
                     /old_locus_tag="OR613_26870"
     CDS             933..1658
                     /locus_tag="OR613_RS26875"
                     /old_locus_tag="OR613_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_074184131.1"
                     /translation="MASMETEQQPQKNIEQIEVKAKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKDASIENVFWFSAKPSHLSRTVNDNYQNYTNALTECGVDVIGGRF
                     KRKNVKCEADNGCGEVFHKHEEKESDVNLAISLVSGACNNEYDIAIIITADSDLCPPIK
                     YVKERFPDKELWLVAPPGRIKRAADLCLMSSKNMEINSNALRASCMRDEFYKGGVLVAR
                     RPQEWRAIANTGQRRTRSR"
                     /name="NYN domain-containing protein"
     gene            complement(1861..1974)
                     /locus_tag="OR613_RS26880"
                     /old_locus_tag="OR613_26875"
     CDS             complement(1861..1974)
                     /locus_tag="OR613_RS26880"
                     /old_locus_tag="OR613_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074184130.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDNAPLAAATGN"
                     /name="replication initiation protein"
     gene            2389..2580
                     /locus_tag="OR613_RS26885"
                     /old_locus_tag="OR613_26880"
     CDS             2389..2580
                     /locus_tag="OR613_RS26885"
                     /old_locus_tag="OR613_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2580..2981
                     /locus_tag="OR613_RS26890"
                     /old_locus_tag="OR613_26885"
     CDS             2580..2981
                     /locus_tag="OR613_RS26890"
                     /old_locus_tag="OR613_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3809..4096
                     /locus_tag="OR613_RS26895"
                     /old_locus_tag="OR613_26890"
     CDS             3809..4096
                     /locus_tag="OR613_RS26895"
                     /old_locus_tag="OR613_26890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074184133.1"
                     /translation="MSDDSSVNLVPVTVFESQVEAVEGITIVVRAPSNTMVPEYTYVRR
                     SPGDNTVGEWMNNRINPSLNGLEVTIIDGEHESPRRSTKRLSTLRDSYER"
                     /name="hypothetical protein"
     Typing          complement(1862..2269)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.195"
                     /coverage="99.0"
                     /evalue="1.29e-162"
ORIGIN
        1 atttaaaata tgtttttttc agaagctcga cattctttgc aatatgaatt tattacttcc
       61 ccagccattg tctttcggat gccaaaaaaa ctaacgatct cttcgtaact ggttgcttta
      121 gtttttaaac atttagggca aagtcgaggt aattttatat tggatatggt tccgatgttc
      181 tttctttctt tctttttgtt tctgctaatt gttatacctt tagatttttt atttaaatgt
      241 tcttttagtg gagttgaatc gaatatattt gccccattgg ttctagggtc attccttgtt
      301 gattggtgtc tggtcaatat tcctaatgat tctagattgc taactccacg ttgaatcgtt
      361 cttttagata cacccattct tgatgcaatg gtaccaattg aaggaaaaga tattgactcc
      421 cctgattcag gccattgatg cattaataca ttcagtagaa tacacatctc agtactccca
      481 atcccaagat cgccttgcag cataaggagg ctgtttggta cagctgtcca cccatgtaga
      541 gttgcatcct ctccccagtg cgtagtgatt aatgactctt ttttttgttc tttctgtgcg
      601 ttttctgtac tcatgggtgc ttttctcgtg aaaaaaaatc aaaacgtgaa cagttactac
      661 ctaactactc ttataaagag tagttaggta gtaaaatgta tctttatagg atgacatata
      721 tgtcatccat ttgctctttt tttatataga aattagtttg agttcactac ctaagagttc
      781 tgctcttagt ggctatccag gtcgaggctt ggaagcgcgt tgtacacaat tcttcctgta
      841 tcggatgcgt ccatacagtg gtctgttaac tgcggttaag agacgcaaaa aggcctgcat
      901 atgcaggcct ttttgctatc tagaggtcaa atatggcaag tatggaaaca gagcagcaac
      961 cacagaaaaa tattgagcaa atcgaagtaa aagcaaagcg agttcagtgt ttcattgatg
     1021 gctataattt atatcatgct attgaagaaa cgggtaataa agctcttcat tgggttgacc
     1081 ttaaatcttt atgtaaaaga taccttccta aagatgcatc gatagagaat gttttttggt
     1141 tttctgccaa gccatcacac ttatcgagaa cggtaaatga taactatcaa aattacacga
     1201 atgctttaac tgaatgtggt gttgatgtta tcggtgggcg ttttaaaaga aaaaatgtaa
     1261 agtgcgaagc tgataatgga tgtggtgagg ttttccataa gcatgaagaa aaagaatccg
     1321 atgttaattt ggctataagc ctagtgtctg gtgcttgtaa taatgagtat gatatagcca
     1381 ttattatcac tgcggattct gacttatgcc ctcctattaa atacgtaaaa gagcgattcc
     1441 ctgataaaga actttggctt gtagctccac cggggcgaat caagcgagcg gcagatctct
     1501 gccttatgtc atcgaaaaat atggagatca acagtaatgc tttgagagct agctgtatgc
     1561 gtgatgagtt ctacaaaggc ggagtgcttg tagccagaag gcctcaggaa tggcgagcta
     1621 ttgccaatac cgggcaaaga agaactcgaa gtcgttaggc tttgttgacg tagctagagt
     1681 aaggagatta atctagctag gttgaaacga aaaaaccgcc ttggcgggcg gtttttcgaa
     1741 ggttcataga gttggcgctc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
     1801 aaacttgttc tttcagttta gccttaatag gcgcatgact tcaagaccac ctcctctaaa
     1861 tcagttacca gtggctgctg ccagtggcgc gttgtcgtgt ctttccgggt tggactcaag
     1921 tcgatagtta ccggataagg cgcagcagtc ggactgaacg gggggttcgt gcatacagtc
     1981 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     2041 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     2101 agccacctat cagaaatgat aggtatcttt atagtcctgt cgggtttcgc caccactgat
     2161 ttgagcgtct gttttctgtg atgcttgtca ggggggcgga gcctatggaa aaacggccgc
     2221 tgcgcggcct ttccttttcc catcacgcga ccgttcggtt ttctcgagct ttatacgcat
     2281 gctcctgccg ctcgccgcag ccgaacggca ggagcgtagc gactgagtga gcgaggaagc
     2341 ggaaaagaga acatccctac attgagcact gacgctctga ggctccttat gacgaaacaa
     2401 cagaaaacag cgctgaacat ggctaaattc atccagaatc agtctctgct cctgctggag
     2461 aagcttaacg agcttgatct tgatgctgag gccgaccttt gtgagaaact ccacgacgat
     2521 gcggagcatc ttttccgtac actgtcctcg agactggatg cattacagga tggtaactga
     2581 tggccagagt gtctattagt gaggctgccc gactgacggg taagagtaga acaacactgc
     2641 acaggttaat taaagcaggt gacctgtcca cctgtagcgg tgaacgcaat gcaaaaatgg
     2701 tagatacttc agagctttta cgtgtctttg gtccctttga acaactgaag ggtgaacacc
     2761 attcaggaca ggtctctgaa cagcgtgata cagcctctgc tgaccagagt gaacaggtga
     2821 ttcgtcatct gaaacaggag attgaacacc tgaacacctt acttttgtcc aaggattcgc
     2881 acattgacag cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca aaattaactg
     2941 aatccccggc gccatggtgg aaattctgga aaaaatcctg accccgtcat gcttcagttt
     3001 tcctctccgg ccatcccggg gagttcctga accttcccaa aaatcgtcag atctctgaac
     3061 gctcctcacc actaaaaata cccacacccc acgcaaaatt aacattttgc ggatttgtat
     3121 ttaatatcag tagcttaagt tggtgaaatt tcgtttttta aatttgattt atatagatcc
     3181 gactctatga cgactcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc
     3241 aggactataa aaacaatgag ttaataacga cccgactcta gacacgactc cccgtttggg
     3301 ggttttttag acgtggcgac tctgggtacg actctagtga agagtcgccg gtgaatcgtg
     3361 tggtgagtcg ttcggtgact tgccataacg tgattgccgg ccgtcgacga acacgattcc
     3421 tgaacgtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     3481 attgcgtccc cgttcaccgt tttcagtcgg gtttggtgcg ggttttgagt tgtctggcgt
     3541 gaattcagga tgaaatctac ctccagccag tctggtgcat aacaattcat gcccgtaaaa
     3601 cccatcagcg cgattttgag gcgttttcag ggggtaagac gtaggattta atgggttgcg
     3661 ccccagaatc gtttctggag cgtttgagcg gtgcgcgtaa tgggacgtta tggtaaatcg
     3721 tacattttga aattttcagt agacatttgt tgggttactc gctaacttcc tgctaactag
     3781 aatttaattg cttggtaagg tcaatataat gtctgatgat tccagtgtta acttagttcc
     3841 tgtgacggtt tttgagtcgc aggttgaagc ggttgagggt attaccatcg tggttagagc
     3901 accatcgaat actatggttc ctgaatatac gtatgttaga cgatcccctg gcgataacac
     3961 cgtaggagag tggatgaaca atagaattaa cccgtcatta aatggccttg aagtcacgat
     4021 tattgatggg gagcatgaat cgcctcgtcg ttcaactaag cgcctatcta ctttacggga
     4081 tagttacgag cgataaccgc tatcaccatg aataagagaa gccgcttata cagcggcttc
     4141 tttatgtggt acgggcaatg aggcgttatg gtaaatgggg caaagtttta attggactct
     4201 gcaccttttc ccatatattt atatgtttta at
//
