LOCUS       NZ_CP111103             3306 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Escherichia coli O157:H7 str. EDL933 plasmid pEDL933-2, complete
            sequence.
ACCESSION   NZ_CP111103
VERSION     NZ_CP111103.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31023063
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7 str. EDL933
  ORGANISM  Escherichia coli O157:H7 str. EDL933
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Peroutka-Bigus,N., Nielsen,D., Trachsel,J., Kudva,I. and Loving,C.
  TITLE     Phenotypic connections with genomic virulence signatures in Shiga
            toxin producing O157 Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Nielsen,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2022) FSEPRU, USDA-ARS, 1920 Dayton Ave, Ames, IA
            50010, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Flye v. 2.7
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 288x
            Sequencing Technology        :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/24/2024 10:44:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP111103.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Escherichia coli O157:H7 str. EDL933"
                     /mol_type="genomic DNA"
                     /strain="EDL933"
                     /isolation_source="ground beef"
                     /host="Bos taurus"
                     /db_xref="taxon:155864"
                     /plasmid="pEDL933-2"
                     /geo_loc_name="USA"
                     /lat_lon="42.0308 N 93.6319 W"
                     /collection_date="1982"
     gene            join(3170..3306,1..904)
                     /gene="lsoA"
                     /locus_tag="ORM96_RS00005"
                     /old_locus_tag="ORM96_00005"
     CDS             join(3170..3306,1..904)
                     /gene="lsoA"
                     /locus_tag="ORM96_RS00005"
                     /old_locus_tag="ORM96_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="WP_000068433.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            901..1281
                     /gene="lsoB"
                     /locus_tag="ORM96_RS00010"
                     /old_locus_tag="ORM96_00010"
     CDS             901..1281
                     /gene="lsoB"
                     /locus_tag="ORM96_RS00010"
                     /old_locus_tag="ORM96_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(1516..1839)
                     /gene="mobA"
                     /locus_tag="ORM96_RS00015"
                     /old_locus_tag="ORM96_00015"
     CDS             complement(1516..1839)
                     /gene="mobA"
                     /locus_tag="ORM96_RS00015"
                     /old_locus_tag="ORM96_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2111..2305
                     /locus_tag="ORM96_RS00020"
                     /old_locus_tag="ORM96_00020"
     CDS             2111..2305
                     /locus_tag="ORM96_RS00020"
                     /old_locus_tag="ORM96_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041624.1"
                     /translation="MNKQQQTALNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2433..2588)
                     /locus_tag="ORM96_RS00025"
     CDS             complement(2433..2588)
                     /locus_tag="ORM96_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318241.1"
                     /translation="MSVDFCDARQGGGAYGKTPAVLASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            2792..2905
                     /locus_tag="ORM96_RS00030"
                     /old_locus_tag="ORM96_00030"
     CDS             2792..2905
                     /locus_tag="ORM96_RS00030"
                     /old_locus_tag="ORM96_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     Typing          complement(1849..2074)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.583"
                     /coverage="99.0"
                     /evalue="1.05e-76"
     Typing          2335..2864
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.413"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1520..1839)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.0199999999999997e-60"
ORIGIN
        1 aatagacttc ttaataaaac taatgccatc aggcggaaat accactatcg gaagagcgtc
       61 tgggcaaaat aacacttact ttgatgaaat cgccttgatt ataaaagaaa actgtttgta
      121 ttcagacaca aagaactttg aatacaccat tccaaaattc agtgatgatg acagggcaaa
      181 tctatttgaa tttctttctg aagaggggat aacaataaca gaagataata ataacgatcc
      241 taattgtaaa caccaatata ttatgaccac cagcaatggt gacagggtca gggcaaaaat
      301 ttacaagcgc ggctctattc aattccaagg aaaatacctt caaatcgcga gtttgattaa
      361 cgatttcatg tgctcaatac taaacatgaa agagattgtc gaacaaaaaa ataaagaatt
      421 taatgttgac ataaaaaaag aaactattga gtccgagttg catagcaaac taccaaaaag
      481 catcgataaa atccatgaag atatcaaaaa acagctatca tgctcgctaa taatgaaaaa
      541 aatagatgtc gaaatggaag attactcaac atactgcttc tctgcattaa gagccataga
      601 aggctttata tatcaaatac ttaatgatgt ttgcaatcca tcatcatcaa agaaccttgg
      661 cgaatacttc actgaaaaca aacccaaata tataatcaga gaaatacacc aagaaactat
      721 aaatggtgaa atagcggaag ttttgtgtga atgctacact tactggcatg agaacaggca
      781 tggtttgttt catatgaaac caggaatagc tgacacgaag acaattaaca aattagaatc
      841 aatcgcaatc atcgataccg tttgccaatt aatagatggt ggcgtagcta ggttgaaatt
      901 atgaaaaaag ataaaaaata tcaaatagaa gcaataaaaa ataaagataa aactttattt
      961 attgtctatg ctactgatat ttatagcccg agcgaatttt tctcaaaaat cgaatccgac
     1021 ttgaagaaaa agaaaagcaa gggtgatgtt ttttttgatt taataattcc taacggtgga
     1081 aaaaaagatc gttacgtcta tacgtcattt aatggcgaga agttttcaag ttacacatta
     1141 aacaaagtta cgaaaactga tgaatataat gatttatctg agctctcggc ttcgttcttt
     1201 aaaaaaaact ttgataagat caacgtaaac cttctatcca aagccacatc atttgctttg
     1261 aaaaaaggca ttccaatata atatcaagcc agaagacatt taccataacg tctcattacg
     1321 cgcaccgcca aaacgcgctg aacgcgattc tgacgcagac accgcaaaaa gagatgtttt
     1381 ccccggggta aaccggaaaa atgcgtcagg atcgctttca gctcactgca tagctatgca
     1441 tgaaagtgaa tggcgatcgg tttgggggct tacggcgttc ataccgtctg ttttcgacag
     1501 tttctctccg ggaagctaat ctgccataag cctggataac agggcacggt gatactccgt
     1561 aatagcgatc agcacctccg catactctct gtccccgaca cgcttgccgt cgataaagag
     1621 ttttttctgc aacgccccca gtcgcataac ttctttcagc acccggtcat cagtcagtga
     1681 gttaactttc ttaccgagag ctgccgcccg taaaaaacca gaaacggtct tgccgcattc
     1741 agcagccctt tttctgattt cctggtcttc ttctgccgtc agacgaacag ggcgactgat
     1801 agcccgcctg cgcgtattac ttccacttcg ttttgtcata aaacatggct ccgtatctga
     1861 catggggtgt cggggcgaag ccctgaccag ggtaattgta atagcgtgca tgtatgcgcg
     1921 gtataacaat tgcacatcct gtcctgtctg caagttcgaa ttaccaacaa agcactgctg
     1981 ttttttggca gtgacagtgt tgtctgactg aaatgtccgt cggcagaata ccggcggtaa
     2041 gacggtcgaa aagtggtgcg cgatataatc acacgacgca gatgacagac agaacggaga
     2101 aaacagaaag atgaacaagc agcagcaaac tgcactcaac atggcgggat tcataaaaag
     2161 ccagagcctg acgctgctcg aaaaactgga cgcactcgat gctgacgagc aggccaccat
     2221 gtgtgagaag ctgcacgaac tcgcagaaga actccagaac agcatacaga cacgctttga
     2281 agtagaaaat agcacagaga tataacgctc agggcaggat gccgaacggc acaacagagc
     2341 gtaaggagaa aatgtcacat aaggcgctct tccgcttcct cgctcactga ctcgctacgc
     2401 tcggtcgttc gactgcggcg agcggtgtca gctcactcaa aggcggtaat acggttatcc
     2461 acagaatcag gggataaagc cggaaagaac atgtgagcaa aaagcaaagc accggaagaa
     2521 gccagcaccg caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2581 acgctcaagc cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2641 cggaagctcc ctcgtgcgct ctcctgttcc gaccctgcca cttaccggat acctgtccgc
     2701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc
     2761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2881 actggcagca gccattggta actgatttag aggagtttgt cttgaagtta tgcacctgtt
     2941 aaggctaaac tgaaagaaca gattttggtg actgcgctcc tccaagccag ttaccttggt
     3001 tcaaagagtt ggtagctcag cgaaccttga gaaaaccacc gttggtagcg gtggtttttc
     3061 tttatttatg agatgatgaa tcaatcggtc tatcaagtca acgaacagct attccgttga
     3121 caaaaaaaca agatctgtac atattcaaaa aaaacaacgg attgacccaa tggcacagaa
     3181 cccgtttaaa gcactaaata tcaatattga caagattgag tctgctctga cgcagaatgg
     3241 cgtcacaaac tattcctcta atgtaaaaaa cgaaagagaa actcacatat ctggcacata
     3301 taaagg
//
