LOCUS       NZ_CP111011             8802 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain J53-p130 plasmid p130-3, complete sequence.
ACCESSION   NZ_CP111011
VERSION     NZ_CP111011.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31669046
            Assembly: GCF_026247865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Evolution and transfer of virulence plasmids of hypervirulent and
            carbapenem resistant Klebsiella pneumoniae strains in hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2022) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Shanghai 272000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:52:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,805
            Genes (coding)                    :: 4,560
            CDSs (with protein)               :: 4,560
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 73 of 245
            Pseudo Genes (incomplete)         :: 181 of 245
            Pseudo Genes (internal stop)      :: 45 of 245
            Pseudo Genes (multiple problems)  :: 53 of 245
            Pseudo Genes (short protein)      :: 4 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP111011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="J53-p130"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p130-3"
                     /geo_loc_name="China: Shandong"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2022-06-30"
                     /collected_by="Dongxing Tian"
     gene            join(6842..8802,1)
                     /locus_tag="ORI87_RS24425"
                     /old_locus_tag="ORI87_24425"
     CDS             join(6842..8802,1)
                     /locus_tag="ORI87_RS24425"
                     /old_locus_tag="ORI87_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1..732
                     /gene="mobC"
                     /locus_tag="ORI87_RS24430"
                     /old_locus_tag="ORI87_24430"
     CDS             1..732
                     /gene="mobC"
                     /locus_tag="ORI87_RS24430"
                     /old_locus_tag="ORI87_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            761..1024
                     /locus_tag="ORI87_RS24435"
                     /old_locus_tag="ORI87_24435"
     CDS             761..1024
                     /locus_tag="ORI87_RS24435"
                     /old_locus_tag="ORI87_24435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(1055..1234)
                     /locus_tag="ORI87_RS24440"
                     /old_locus_tag="ORI87_24440"
     CDS             complement(1055..1234)
                     /locus_tag="ORI87_RS24440"
                     /old_locus_tag="ORI87_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1303..1680
                     /locus_tag="ORI87_RS24445"
                     /old_locus_tag="ORI87_24445"
     CDS             1303..1680
                     /locus_tag="ORI87_RS24445"
                     /old_locus_tag="ORI87_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(1742..2041)
                     /locus_tag="ORI87_RS24450"
                     /old_locus_tag="ORI87_24450"
     CDS             complement(1742..2041)
                     /locus_tag="ORI87_RS24450"
                     /old_locus_tag="ORI87_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2031..2294)
                     /locus_tag="ORI87_RS24455"
                     /old_locus_tag="ORI87_24455"
     CDS             complement(2031..2294)
                     /locus_tag="ORI87_RS24455"
                     /old_locus_tag="ORI87_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(2600..2845)
                     /locus_tag="ORI87_RS24460"
                     /old_locus_tag="ORI87_24460"
     CDS             complement(2600..2845)
                     /locus_tag="ORI87_RS24460"
                     /old_locus_tag="ORI87_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3172..4857
                     /locus_tag="ORI87_RS24465"
                     /old_locus_tag="ORI87_24465"
     CDS             3172..4857
                     /locus_tag="ORI87_RS24465"
                     /old_locus_tag="ORI87_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_266051493.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQDADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4867..5124
                     /locus_tag="ORI87_RS24470"
                     /old_locus_tag="ORI87_24470"
     CDS             4867..5124
                     /locus_tag="ORI87_RS24470"
                     /old_locus_tag="ORI87_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5209..5358
                     /locus_tag="ORI87_RS24475"
                     /old_locus_tag="ORI87_24475"
     CDS             5209..5358
                     /locus_tag="ORI87_RS24475"
                     /old_locus_tag="ORI87_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..8802
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5462..6040)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..729
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 atggcactgg agcgatacaa cgtcagccac gcgaagcggc aggcacggaa cgcggagaaa
       61 acacggctga ccctgcgctg gctgcgggaa gaactgtgtt ccacggcgga gctggttgcg
      121 caacggctgg ggattgcggc ggtacagccg gtttaccgct tcctggacag cctggtggcg
      181 aaagggctgc tggtccgggc gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg
      241 ctgacgccgc acggggtggc gttctcgttc gacgaggacg agccgctgac ggacattatt
      301 ccgtttcagc cgtcgcgggt gtcggcggcg cagctgccgc accggctggc cgtgcagtcc
      361 ctgcgcctcg ccatggaggc ccggggggcc accggctggc ggtacctgca ccgtattgcc
      421 ctgaaaggga tgaaggtgcc ggacgcgctg gcggagctgg acggcagaac ggtggcgttt
      481 gaggtggaac gcaccgtgaa gagccgcagg cgctatcagg aggtggtgtc cgggtatctg
      541 tttaaccgcc gggcgaacgg catcgatgaa atctggtaca tttgcccgga ccgggcgacg
      601 caggtcaggg tgcaacgggc tattctggcg gtggatgaaa tcgtgaaccc gcagaccggc
      661 gaggcccgca aaacggtgga gctggaccgg gagcggctgt ttgcgtgctt taaattcatg
      721 atgatggact gagcttatac attcactcag agaggttttt atgacaaatc gtgttgttgc
      781 cgtggggcgt gaagccctgt tgatggtcgc tgtcatggcc gggacgctgg tcgttctggc
      841 cggaaacctg ctgaaactgc tttggcgcgt actgccggtg cttctgctgc tctacggggt
      901 tgtgcagttt ctggcgagga acccggagat ccgtgactgg ctgtttctgc atttcgggca
      961 gggaggcacg gcggccattc ttgtcggggg aatactcctc tgtatgcgcc gcctgatgaa
     1021 ataatgccgg acagcaccgg ctcggccggt gctgttattc ctccccgaga tggtcccgca
     1081 ttgccagcca gagcttttct gcatcctcat gcagccgttc gcacatgtcg gccggggtat
     1141 ccatatccag ctcgttcagt ttctcgagca acagcagcga ctggtcctta atgaatttcg
     1201 ccatgttgag ggcggttttt tcctgcttcg tcatgtttct gtccttccgg gcacggtctg
     1261 cgtctgtcgg gtatactgtc tcaaaaatag cggggtgaga caatggcgtg ggtgacggtg
     1321 agacaggcga cggaactgac ggggaaagcc cggagttccc tgtatcggga tatggcgaaa
     1381 ggccgcgtat cataccgtac ggaagctgat ggtggtcggg tgatggatac cagcgagctt
     1441 atccgggtgt acggtgagtt aagacagggt gagactcacg agcgggacgg gttgagactg
     1501 ccggatgaga ctgaaaaaac ggccttcgac gcgttgatcg cggagataaa agcactcagg
     1561 gaggaggtgg ccggactgcg gcaggaaatg caggccatga gactgctgga acacaaaccg
     1621 gagacagcag agacaccggc cggaacaccg cgccggtggt ggcagtgggg aaaacgctga
     1681 cgcgctgtgc ttgtccaacc ggcgacggcc acgcaatgaa ttgcgccccc gtcaccggtt
     1741 atcagcgaac ggtatgggga ttctggcgct ggccaaccac ggccagaacc cggactttcc
     1801 tggtctggtc atcaacatca tacaggacat gctgtcccat cagactgatc cggcgaacgt
     1861 cctcgccata gcgtgagtcc acttcataga ggcgcgggaa gtcggccagt ttgtccgccg
     1921 cagtgagaac gcgatcggta acgttctgcg catcccacag ggatgaattt tccgcgatat
     1981 agtcaaacag cgtcgttaca tcctcgatgg ctccctgttt ccattccacc tcatacggca
     2041 tcgcgcagtt cctgataacg gccatgcagg cgctggcgga gttgttccgt ggtcatgccc
     2101 ggacgcgtgt cagcacgggc ggcggttact ttggcgcgaa cctgatcttc atactctgcc
     2161 tgggccagtt cttcgttagc caggcattgc aacaacgcag ctttgtccgg gccactttcc
     2221 ggcagtatgc ggggcaggtt ttcagccagt tcccgcagga agttggcgtt ttcgtagtgt
     2281 gtagtgttgc tcattgcgga gactcccgga ttaatggggt gatatgaaga agtttagcag
     2341 ggggcggtct gaaagaatat gctcgtgtca gtcaggctga tgaagacgaa agctggtaaa
     2401 aaagggctta cggcacccat tttacgtcaa actgcacgcc atgcagttgc gtgcataggc
     2461 atgcattagg ataaagttta ccgggcgcgt ttccggcggt tttccgggtg gtttgttgcc
     2521 tgttttatcc cgtagccgcc ggaaatgccc tcagaccgtc tgagcggtgc gcgtaatgcg
     2581 acgttatggt aaatacccct taccggcggc gctgtggctg ccaggtgaag gtggtttccg
     2641 tagcctcgta tttctcccga cgcatttcct gcgcccgcct ggcttcacgt tcgtcctgaa
     2701 tggcacgaag caccctttcc cgcacctgtt gttttaccgc ctttggcagc gttttgttcc
     2761 ctttcaggcc catggccagt gcctgagcga caaaggcgtc cgccaggcgc ttttcttcag
     2821 cgggagtcag tttcaggtgc gtcatagtgg cctccgggcg tctcaggttt cccgaacagt
     2881 ttaaccgcag gtcctctcct catgccagtt agctttcaga tgtgtctata ccatctgaaa
     2941 tgtaagtgtg atgaatgtct taaataataa aatttgttgc catgagaaat tttcgggaaa
     3001 agacgcgatc tgcttacagg tggctccggg cgcttttttg tgcctgaaga tgagaactaa
     3061 tctcatatat tgacacctga aaacgggagg agtaaggtaa taattatact gtgtatatgt
     3121 acagtatatt ggctttaatt tattgtttta aatgtcaaag aggaaaacga tatgagtggt
     3181 ggagacggtc gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg
     3241 aatgtgaacg gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac
     3301 agcgggccgg gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg
     3361 ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat
     3421 agcggtagct ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc
     3481 ctggctactc cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc
     3541 gccgttgctg atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt
     3601 gcgatctacg gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa
     3661 attatgacgt cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg
     3721 gaccaggcga cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag
     3781 catattgcgg ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca
     3841 aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta
     3901 ccgaaaggtg ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat
     3961 tcacgtccgg ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg
     4021 aaagagaccg gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag
     4081 gtaaaacagc gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg
     4141 gaagaggggc tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa
     4201 aatattgcga ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg
     4261 gctgctgttc aggacgccga taaaaaggta aaagaggcag aggcaaataa agatgatttt
     4321 gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt
     4381 gataaggata ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca
     4441 atgaatgaat ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa
     4501 caaaaggaga aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct
     4561 cgcaagggga ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata
     4621 aagggacttg gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt
     4681 aagcgggccc gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac
     4741 ggtgagcttg aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa
     4801 acgggtaagc aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag
     4861 gtaaatatgg ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa
     4921 ggggaagagt attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg
     4981 cctttaaagg ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta
     5041 ctacagccat tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt
     5101 gattatcggg acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt
     5161 ccggtgttca gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta
     5221 aagactattt ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca
     5281 aattatatcc gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg
     5341 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5401 taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag
     5461 tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct
     5521 gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa
     5581 acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt
     5641 tcccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg
     5701 gagttcttgc ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg
     5761 tgtgagctat gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt
     5821 aaacggcagg gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga
     5881 tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc
     5941 ccacagatgc accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat
     6001 ggaaaaacgc caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc
     6061 cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg
     6121 agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc
     6181 ggacgcgctg atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat
     6241 tatcaggata ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg
     6301 ttgctttttt ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca
     6361 ggggaaaaga ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt
     6421 tctggctctg tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct
     6481 cacgcctgac cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct
     6541 gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac
     6601 accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt
     6661 gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa
     6721 agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg
     6781 cttgtgcagc gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg
     6841 catgtttaat acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat
     6901 ttacccgtgg gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt
     6961 tatcggcctg tttttcggtc tggcagaact ctgggccggg cggatgcgat tcgtggggct
     7021 gagtcataag gagcggcggg agtatgccga tcgctataag cgcgataata accggactgc
     7081 gccattttcg gtgctggcct cgctggattc gcccgggcgg atggtcaggg cacggcgaca
     7141 tgtctggaac tacgcatact ggctgaccgg cgctgccggt ctggcccttc tgggggggct
     7201 ggttgtcgcc gtgtttatcg agagctgggg agaaaccgtc ctgcgtccgg acctctacgc
     7261 gaacgcagat tattacctgc tgcagccggc taacctgttc gggattgcct ggtgtgccgg
     7321 aggtcttgtc gcaggcgtcc tcctggcgcg gctgctggcg ttcctgctgc ttgacgggca
     7381 ttttgcggcc gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag
     7441 ccagcggacc ggagaaatga ccgacgtccg ccacctgcat ttcggggagc cggtaccggt
     7501 taacgcgctg gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg
     7561 taaggatgag cagggccagc cggtgctggt cccgcgtgat acctggcgca agaccaacat
     7621 tcagatcctc ggcctgcccg ggagcggcaa gagcgtgatg gccaccaacg cgctcattcg
     7681 ctgcgtacgc gatttcgggg acgcggtggt gtacttcgac ccgaaagggg acgcgtgggc
     7741 gccgcacgtc ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa
     7801 accggcgcag ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atcttctggt
     7861 tgccggattt aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca
     7921 gaaagcggcg aagctgattg cggagcagtt tccgcagggg gcaaatattc agcaggtgct
     7981 ggcggcggcg tacgcactgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa
     8041 gctggagaac gtggccgacc tgagcgtcct tcagacggac agcggcatcg acgtggcggg
     8101 gataatcaac ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat
     8161 ccgggtgcag aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggacgagtt
     8221 ccggaggtgg ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta
     8281 cgtgctgaac gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca
     8341 gtcgctgggc gacttcggcc agtgcgggca ggatctgccc gctgattttg tgaaaaccac
     8401 ggtactggat aacacgccga tccgctggtt ctaccgggcg gccagccagg agtccgctca
     8461 gtgggcggcc ggacagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg
     8521 ggaggcgggg aacgtggagc atatcagcgg ggacagcttc atccagaagg aggcgcggcc
     8581 gctgtttgac gtgaacaccc tgcaacatct gcctgacggg ttcgcggtga tgaccgggct
     8641 gggcgtggcg cggctggcgt tcagcagccc gctgcgtgtt gatcgccggg aaattccgct
     8701 gaaaaccttc cctgtgctgg cgaaaacgga cccgctggcg gagtaccagt cggaggccgg
     8761 gcggcagcgg ccgggtgatg atgattttgc ggggctgtac tg
//
