LOCUS       NZ_CP111007             6883 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain J53-p131 plasmid p131-3, complete sequence.
ACCESSION   NZ_CP111007
VERSION     NZ_CP111007.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31669047
            Assembly: GCF_026247845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Tian,D.
  TITLE     Evolution and transfer of virulence plasmids of hypervirulent and
            carbapenem resistant Klebsiella pneumoniae strains in hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2022) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Shanghai 272000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:29:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,596
            CDSs (with protein)               :: 4,596
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 71 of 246
            Pseudo Genes (incomplete)         :: 183 of 246
            Pseudo Genes (internal stop)      :: 44 of 246
            Pseudo Genes (multiple problems)  :: 51 of 246
            Pseudo Genes (short protein)      :: 4 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP111007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="J53-p131"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p131-3"
                     /geo_loc_name="China: Shandong"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2022-06-30"
                     /collected_by="Dongxing Tian"
     ARG             complement(2722..3062)
                     /name="ANT(4')-Ia"
                     /gene="ANT(4')-Ia"
                     /gene_name="ANT(4')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.04"
                     /coverage="44.66"
     ARG             complement(2292..2755)
                     /name="ANT(4')-Ia"
                     /gene="ANT(4')-Ia"
                     /gene_name="ANT(4')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.68"
                     /coverage="60.87"
     Typing          complement(3223..4234)
                     /name="rep_cluster_1018"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.013"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctcacaagga ggcggatatg atatgaggcg tttgctggac ggtaccgttg tgtccaggca
       61 tccggaaaag gcggttacat ttgttgaaaa tcatgacaca cagccgggac agtcattgga
      121 atcgacagtc caaacttggt ttaaaccgct tgcatacgcc tttattttga caagagaatc
      181 cggttatcct caggtgttct atggggatat gtacgggaca aaagggacat cgccaaagga
      241 aattccctca ctgaaagata atatagagcc gattttaaaa gcgcgtaagg agtacgcata
      301 cgggccccag cacgattata ttgaccaccc ggatgtgatc ggatggacga gggaaggtga
      361 cagctccgcc gccaaatcag gtttggccgc tttaatcacg gacggacccg gcggatcaaa
      421 gcggatgtat gccggcctga aaaatgccgg cgagacatgg tatgacataa cgggcaaccg
      481 ttcagatact gtaaaaaatc ggatctgacg gctggggaga gtttcatgta aacgatgggt
      541 ccgtctccat ttatgttcag aaataaggta ataaaaaaaa cacctccaag ctgagtgcgg
      601 gtatcagctt ggaggtgcgt ttattttttt cagccgtatg acaaggtcgg catcaggtgt
      661 gacaaatacg gtatgctggc tgtcataggt gacaaatccg ggttttgcgc cgtttggctt
      721 ttttcacatg tctgattttt tgtataatca acaggcacgg agccggaatc tttcgccttg
      781 gaaaaataag cggcgatcgt agctgcttcc aatatggatt gttcatcggg atcgctgctt
      841 ttaatcacaa cgtgggatcc agttgctcaa aaaaaatctc ggtcagatgt tactagcaac
      901 tcatttacaa gaacagcatc tttcctcgtt ttttcttgta cctgtttttt tgtgattcaa
      961 taatttcttt gacacgttcg ttgtaatcaa tatttttatc atttttcaaa tcataatttt
     1021 cacgtgttcg ctcatggtca atatcatcat tcgttctact tttttcgctc tctttgatta
     1081 tgaaattgca tgccttttag tccagctgat ttcacttttt gcattctaca aactgcataa
     1141 ctcatatgta aatcgctcct tttttaggtg gcacaaatgt gaggcatttt cgctctttcc
     1201 ggcaaccact tccaagtaaa gtataacaca ctatacttta tattcataaa gtgtgtgctc
     1261 tgcgaggctg tcggcagtgc cgaccaaaac cataaaacct ttaagacctt tctttttttt
     1321 tacgagaaaa aaaagaaaca aaaaaaacct gccctctgcc acctcagcaa aggggggggt
     1381 tttgctctcg tgctcgttta aaaatcagca agggacaggt agtatttttt tgagaagatc
     1441 actcaaaaaa aatctccacc tttaaaccct tgccaatttt ttattttgtc cgttttgtct
     1501 agcttaccga aagccagact cagcaagaat aaaatttttt attgtctttc ggttttctag
     1561 tgtaacggac aaaaccactc aaaataaaaa aagatacaag agaggtctct cgtatctttt
     1621 tattcagcaa tcgcgcccga ttgctgaaca gattaataat agattttagc tttttatttg
     1681 ttgaaaaaaa gctaatcaaa ttgttgtcgg gatcaattac tgcaaagtct cgttcatccc
     1741 accactgatc ttttaatgat gtattggggt gcaaaatgcc caaaggctta atatgttgat
     1801 ataattcatc aattccctct acttcaatgc ggcaactagc agtaccagca ataaacgact
     1861 ccgcacctgt acaaaccggt gaatcattac tacgagagcg ccagccttca tcacttgcct
     1921 cccatagatg aatccgaacc tcattacaca ttagaactgc gaatccatct tcatggtgaa
     1981 ccaaagtgaa acctagttta tcgcaataaa aaacctatac tcttttttaa tatccccgac
     2041 tggcaatgcc gggatagact gtaacattct cacgcataaa atcccctttc attttctaat
     2101 gtaaatctat taccttatta ttaattcaat tcgctcataa ttaatccttt tttcttatta
     2161 cgcaaaatgg cccgatttaa gcacaccctt tattccgtta atgcgccatg acagccatga
     2221 taattactaa tactaggaga agttaataaa tacgtaacca acatgattaa caattattag
     2281 aggtcatcgt tcaaaatggt atgcgttttg acacatccac tatatatccg tgtcgttctg
     2341 tccactcctg aatcccattc cagaaattct ctagcgattc cagaagtttc tcagagtcgg
     2401 aaagttgacc agacattacg aactggcaca gatggtcata acctgaagga agatctgatt
     2461 gcttaactgc ttcagttaag accgaagcgc tcgtcgtata acagatgcga tgatgcagac
     2521 caatcaacat ggcacctgcc attgctacct gtacagtcaa ggatggtaga aatgttgtcg
     2581 gtccttgcac acgaatatta cgccatttgc ctgcatattc aaacagctct tctacgataa
     2641 gggcacaaat cgcatcgtgg aacgtttggg cttctaccga tttagcagtt tgatacactt
     2701 tctctaagta tccacctgaa tcataaatcg gcaaaataga gaaaaaattg accatgtgta
     2761 agcggccaat ctgattccac ctgagatgca taatctagta gaatctcttc gctatcaaaa
     2821 ttcacttcca ccttccactc accggttgtc cattcatggc tgaactctgc ttcctctgtt
     2881 gacatgacac acatcatctc aatatccgaa tagggcccat cagtctgacg accaagagag
     2941 ccataaacac caatagcctt aacatcatcc ccatatttat ccaatattcg ttccttaatt
     3001 tcatgaacaa tcttcattct ttcttctcta gtcattatta ttggtccatt cactattctc
     3061 attccctttt cagataattt tagatttgct tttctaaata agaatatttg gagagcaccg
     3121 ttcttattca gctattaata actcgtcttc ctaagcatcc ttcaatcctt ttaataacaa
     3181 ttatagcatc taatcttcaa caaactggcc cgtttgttga actactcttt aataaaataa
     3241 ttttttccgt tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc
     3301 gtcatcatct gtatgaatca aatcgccttc ttctgtgtca tcaaggttta atttttttat
     3361 gtatttcttt taacaaacca ccataggaga ttaacctttt acggtgtaaa ccttcctcca
     3421 aatcagacaa acgtttcaaa ttcttttctt catcatcggt cataaaatcc gtatccttta
     3481 caggatattt tgcagtttcg tcaattgccg attgtatatc cgatttatat ttattttttc
     3541 ggtcgaatca tttgaacttt tacatttgga tcatagtcta atttcattgc cttttttcca
     3601 aaattgaatc cattgttttt tgattcacgt agttttctgt attcttaaaa taagttggtt
     3661 ccacacatac caatacatgc atgtgctgat tataagaatt atctttatta tttattgtca
     3721 cttccgttgc acgcataaaa ccaacaagat tttttattaa ttttttttat attgcatcat
     3781 tcggcgaaat ccttgagcca tatctgacaa actcttattt aattcttcgc catcataaac
     3841 atttttaact gttaatgtga gaaacaacca acgaactgtt ggcttttgtt taataacttc
     3901 agcaacaacc ttttgtgact gaatgccatg tttcattgct ctcctccagt tgcacattgg
     3961 acaaagcctg gatttacaaa accacactcg atacaacttt ctttcgcctg tttcacgatt
     4021 ttgtttatac tctaatattt cagcacaatc ttttactctt tcagcctttt ttaaattcaa
     4081 gaatatgcag aagttcaaag taatcaacat tagcgatttt cttttctctc catggtctca
     4141 cttttccact tttttgtctt gtccactaaa acccttgatt ttttcatctg aataaatgct
     4201 actattagga cacataatat taaaagaaac cccccatcta tttagttatt tgtttagtca
     4261 cttataactt taacagatgg ggtttttctg tgcaaccaat tttaagggtt ttcaatactt
     4321 taaaacacat acataccaac acttcaacgc acctttcagc aactaaaata aaaaatgacg
     4381 ttatttctat atgtatcaag ataagaaaga acaagttcaa aaccatcaaa aaaaagacac
     4441 cttttcaggt gctttttttt attttataaa ctcattccct gatctcgact tcgttctttt
     4501 ttttacctct cggttatgag ttagttcaaa ttcgttcttt tttaggttct aaatcgtgtt
     4561 ttttcttgga attgtgctgt tttatccttt accttgtcta caaacccctt aaaaacgttt
     4621 ttaaaggctt ttaagccgtc tgtacgttcc ttaaggaatt ccttagtgct ttcatagatt
     4681 aaactcacat cacgctttaa atcgcttatt ttagacttta aagacttgtt ttcttcaagc
     4741 aactcattat aatcatttac attttcatta aatcgctcta caagaccact atattttttc
     4801 tttaacttgc ccatgttctt tacttaattt ttttatattc tctcgccata tcagtactca
     4861 tgagatttct aacatgctgt tttaacctat cgttatctct cgcgagcagt cactaagttt
     4921 tttataatca cgctccgata taacaacatt ttttggttgg tttcttttct gttttcatta
     4981 tttcttttcc caaaccaaac atggactttt cacccgttgg cacttcaaca cttttcatgt
     5041 gtcgtttcgc tggtacttct aaatctgatt taactttatc gctataagca gtccattcat
     5101 ctttttttaa ctgctaaatt ttttttctag aaaatcaatc tcttttttcc aaagtttgtt
     5161 tttttaaatt tagctgtctc aatatgttta cggtcagagc cacgttcacc acgcttcaac
     5221 tcaaaaccct gtttttttca tatgctcggg gaatttatct tgtagccata acagttcttg
     5281 acgattaaac acattttttt ccttgcagtt ttccatcacg cataggcaca acacctaaat
     5341 gcatgtgagg ggtttgctca tcattatgaa ctgttgcata agcaatattt tgcttgccat
     5401 atcgttcgga aaataattta taactttcct caaaaaaaat cgtttttgtt ctcctggatc
     5461 tcaagcccgc tttttttcat tattgccgcg acaaatgacc gaggcgtgaa tcaggagata
     5521 gccgcaaacg cttctgaaac gcagctggtc aactgtgtaa gcaaggctga acaaggcagc
     5581 gtatatatgc cgaagatcat ccgcaaaggg cgcattcaag tatcagtatc aacaagcggg
     5641 gcaagccccg cacatacgaa aagactggct gaaaacattg agcctttgat gactgatgat
     5701 ttggctgaag aagtggatcg attgtttgag aaaagaagaa gaccataaaa aataccttgt
     5761 ctgtcatcag acagggtatt ttttttatgc tgtccagact gtccgctgtg taaaaaaaat
     5821 aggaataaag gggggggtgt tattatttta ctgatatgta aaatataatt tgtataagaa
     5881 aatgagaggg agaggaaaca tgattcaaaa aacgaaagcg gacagtttcg ttcagacttg
     5941 tgcttatgtg cacgctgtta tttgtcagtt tgccgattac aaaaaacatc agccgtaaat
     6001 ggcacgctga tgcagtattt tgaatggtat acgccgaacg acggccagca ttggaaacga
     6061 ttgcagaatg atgcggaaca tttatcggat atcggaatca ctgccgtctg gattcctccc
     6121 gcatacaaag gattgagcca atccgataac ggatacagac cttatgattt gtatgattta
     6181 ggagaattcc agcaaaaaag ggacggtcag aacgaaatac ggcacaaaat cagagcttca
     6241 agatgcgatc ggctcactgc attcccggaa cgtccaagta tacggagatg tggttttgaa
     6301 tcataaggct ggtgctgatg caacagaaga tgtaactgcc gtcgaagtca atccggccaa
     6361 tagaaatcag gaaacttcgg aggaatatca aatcaaagcg tggacggatt ttcgttttcc
     6421 gggccgtgga aacacgtaca gtgattttaa atggcattgg tatcatttcg acggagcgga
     6481 ctgggatgaa tcccggaaga tcagccgcat ctttaagttt cgtggggaag gaaaagcgtg
     6541 ggattgggaa gtatcaagtg aaaacggcaa ctatgactat ttaatgtatg ctgatgttga
     6601 ctacgaccac cctgatgtcg tggcagagac aaaaaaaatg gggtatctgg tatgcgaatg
     6661 aactgtcatt agacggcttc cgtattgatg ccgccaaaca tattaaattt tcatttctgc
     6721 gtgattgggt tcaggcggtc agacaggcga cgggaaaaga aatgtttacg gttgcggagt
     6781 attggcagaa taatgccggg aaactcgaaa actacttgaa taaaacaagc tttaatcaat
     6841 ccgtgtttga tgttccgctt catttcaatt tacaggcggc ttc
//
