LOCUS       NZ_CP111003             6813 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain J53-p133 plasmid p133-4, complete sequence.
ACCESSION   NZ_CP111003
VERSION     NZ_CP111003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31669048
            Assembly: GCF_026247825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Tian,D.
  TITLE     Evolution and transfer of virulence plasmids of hypervirulent and
            carbapenem resistant Klebsiella pneumoniae strains in hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2022) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Shanghai 272000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:37:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,924
            CDSs (total)                      :: 4,805
            Genes (coding)                    :: 4,560
            CDSs (with protein)               :: 4,560
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 73 of 245
            Pseudo Genes (incomplete)         :: 181 of 245
            Pseudo Genes (internal stop)      :: 45 of 245
            Pseudo Genes (multiple problems)  :: 53 of 245
            Pseudo Genes (short protein)      :: 4 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP111003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="J53-p133"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p133-4"
                     /geo_loc_name="China: Shandong"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2022-06-30"
                     /collected_by="Dongxing Tian"
     ARG             1166..1932
                     /name="aadD1"
                     /gene="aadD1"
                     /gene_name="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2153..2553
                     /name="bleO"
                     /gene="bleO"
                     /gene_name="bleomycin binding protein"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1166..1935
                     /name="ANT(4')-Ia"
                     /gene="ANT(4')-Ia"
                     /gene_name="ANT(4')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.21"
                     /coverage="101.19"
     Typing          2..1005
                     /name="rep_cluster_1018"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5610..6154
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.901"
                     /coverage="100.0"
                     /evalue="8.54e-169"
     Typing          6157..6587
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="100.0"
                     /evalue="8.54e-169"
ORIGIN
        1 atgggggttt cttttaatat tatgtgtcct aatagtagca tttattcaga tgaaaaatca
       61 agggttttag tggacaagac aaaaagtgga aaagtgagac catggagaga aaagaaaatc
      121 gctaatgttg attactttga acttctgcat attcttgaat ttaaaaaggc tgaaagagta
      181 aaagattgtg ctgaaatatt agagtataaa caaaatcgtg aaacaggcga aagaaagttg
      241 tatcgagtgt ggttttgtaa atccaggctt tgtccaatgt gcaactggag gagagcaatg
      301 aaacatggca ttcagtcaca aaaggttgtt gctgaagtta ttaaacaaaa gccaacagtt
      361 cgttggttgt ttctcacatt aacagttaaa aatgtttatg atggcgaaga attaaataag
      421 agtttgtcag atatggctca aggatttcgc cgaatgatgc aatataaaaa aattaataaa
      481 aatcttgttg gttttatgcg tgcaacggaa gtgacaataa ataataaaga taattcttat
      541 aatcagcaca tgcatgtatt ggtatgtgtg gaaccaactt attttaagaa tacagaaaac
      601 tacgtgaatc aaaaacaatg gattcaattt tggaaaaagg caatgaaatt agactatgat
      661 ccaaatgtaa aagttcaaat gattcgaccg aaaaataaat ataaatcgga tatacaatcg
      721 gcaattgacg aaactgcaaa atatcctgta aaggatacgg attttatgac cgatgatgaa
      781 gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt tacaccgtaa aaggttaatc
      841 tcctatggtg gtttgttaaa agaaatacat aaaaaattaa accttgatga cacagaagaa
      901 ggcgatttga ttcatacaga tgatgacgaa aaagccgatg aagatggatt ttctattatt
      961 gcaatgtgga attgggaacg gaaaaattat tttattaaag agtagttcaa caaacgggcc
     1021 agtttgttga agattagatg ctataattgt tattaaaagg attgaaggat gcttaggaag
     1081 acgagttatt aatagctgaa taagaacggt gctctccaaa tattcttatt tagaaaagca
     1141 aatctaaaat tatctgaaaa gggaatgaga atagtgaatg gaccaataat aatgactaga
     1201 gaagaaagaa tgaagattgt tcatgaaatt aaggaacgaa tattggataa atatggggat
     1261 gatgttaagg ctattggtgt ttatggctct cttggtcgtc agactgatgg gccctattcg
     1321 gatattgaga tgatgtgtgt catgtcaaca gaggaagcag agttcagcca tgaatggaca
     1381 accggtgagt ggaaggtgga agtgaatttt gatagcgaag agattctact agattatgca
     1441 tctcaggtgg aatcagattg gccgcttaca catggtcaat ttttctctat tttgccgatt
     1501 tatgattcag gtggatactt agagaaagtg tatcaaactg ctaaatcggt agaagcccaa
     1561 acgttccacg atgcgatttg tgcccttatc gtagaagagc tgtttgaata tgcaggcaaa
     1621 tggcgtaata ttcgtgtgca aggaccgaca acatttctac catccttgac tgtacaggta
     1681 gcaatggcag gtgccatgtt gattggtctg catcatcgca tctgttatac gacgagcgct
     1741 tcggtcttaa ctgaagcagt taagcaatca gatcttcctt caggttatga ccatctgtgc
     1801 cagttcgtaa tgtctggtca actttccgac tctgagaaac ttctggaatc gctagagaat
     1861 ttctggaatg ggattcagga gtggacagaa cgacacggat atatagtgga tgtgtcaaaa
     1921 cgcataccat tttgaacgat gacctctaat aattgttaat catgttggtt acgtatttat
     1981 taacttctcc tagtattagt aattatcatg gctgtcatgg cgcattaacg gaataaaggg
     2041 tgtgcttaaa tcgggccatt ttgcgtaata agaaaaagga ttaattatga gcgaattgaa
     2101 ttaataataa ggtaatagat ttacattaga aaatgaaagg ggattttatg cgtgagaatg
     2161 ttacagtcta tcccggcatt gccagtcggg gatattaaaa agagtatagg tttttattgc
     2221 gataaactag gtttcacttt ggttcaccat gaagatggat tcgcagttct aatgtgtaat
     2281 gaggttcgga ttcatctatg ggaggcaagt gatgaaggct ggcgctctcg tagtaatgat
     2341 tcaccggttt gtacaggtgc ggagtcgttt attgctggta ctgctagttg ccgcattgaa
     2401 gtagagggaa ttgatgaatt atatcaacat attaagcctt tgggcatttt gcaccccaat
     2461 acatcattaa aagatcagtg gtgggatgaa cgagactttg cagtaattga tcccgacaac
     2521 aatttgatta gcttttttca acaaataaaa agctaaaatc tattattaat ctgttcagca
     2581 atcgggcgcg attgctgaat aaaagatacg agagacctct cttgtatctt ttttattttg
     2641 agtggttttg tccgttacac tagaaaaccg aaagacaata aaaattttat tcttgctgag
     2701 tctggctttc ggtaagctag acaaaacgga caaaataaaa attggcaagg gtttaaaggt
     2761 ggagattttt tgagtgatct tctcaaaaaa tactacctgt cccttgctga tttttaaacg
     2821 agcacgagag caaaaccccc ctttgctgag gtggcagagg gcaggttttt ttgtttcttt
     2881 tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt tttggtcggc actgccgaca
     2941 gcctcgcaga gcacacactt tatgaatata aagtatagtg tgttatactt tacttggaag
     3001 tggttgccgg aaagagcgaa aatgcctcac atttgtgcca cctaaaaagg agcgatttac
     3061 atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc agctggacta aaaggcatgc
     3121 aatttcataa tcaaagagag cgaaaaagta gaacgaatga tgatattgac catgagcgaa
     3181 cacgtgaaaa ttatgatttg aaaaatgata aaaatattga ttacaacgaa cgtgtcaaag
     3241 aaattattga atcacaaaaa acaggtacaa gaaaaacgag gaaagatgct gttcttgtaa
     3301 atgagttgct agtaacatct gaccgagatt ttttgagcaa ctggatccca cgttgtgatt
     3361 aaaagcagcg atcccgatga acaatccata ttggaagcag ctacgatcgc cgcttatttt
     3421 tccaaggcga aagattccgg ctccgtgcct gttgattata caaaaatcag acatgtgaaa
     3481 aagccaaacg gcgcaaaacc cggatttgtc acctatgaca gccagcatac cgtatttgtc
     3541 acacctgatg ccgaccttgt catacggctg aaaaaataaa cgcacctcca agctgatacc
     3601 cgcactcagc ttggaggtgt ttttttatta ccttatttct gaacataaat ggagacggac
     3661 ccatcgttta catgaaactc tccccagccg tcagatccga tttttacagt atctgaacgg
     3721 ttgcccgtta tgtcatacca tgtctcgccg gcatttttca ggccggcata catccgcttt
     3781 gatccgccgg atccgtccgt gattaaagcg gccaaacctg atttggcggc ggagctgtca
     3841 ccttccctcg tccatccgat cacatccggg tggtcaatat aatcgtgctg gggcccgtat
     3901 gcgtactcct tacgcgcttt taaaatcggc tctatattat ctttcagtga gggaatttcc
     3961 tttggcgatg tcccttttgt cccgtacata tccccataga acacctgagg ataaccggat
     4021 tctcttgtca aaataaaggc gtatgcaagc ggtttaaacc aagtttggac tgtcgattcc
     4081 aatgactgtc ccggctgtgt gtcatgattt tcaacaaatg taaccgcctt ttccggatgc
     4141 ctggacacaa cggtaccgtc cagcaaacgc ctcatatcat atccgcctcc ttgtgaggaa
     4201 gccgcctgta aattgaaatg aagcggaaca tcaaacacgg attgattaaa gcttgtttta
     4261 ttcaagtagt tttcgagttt cccggcatta ttctgccaat actccgcaac cgtaaacatt
     4321 tcttttcccg tcgcctgtct gaccgcctga acccaatcac gcagaaatga aaatttaata
     4381 tgtttggcgg catcaatacg gaagccgtct aatgacagtt cattcgcata ccagataccc
     4441 catttttttg tctctgccac gacatcaggg tggtcgtagt caacatcagc atacattaaa
     4501 tagtcatagt tgccgttttc acttgatact tcccaatccc acgcttttcc ttccccacga
     4561 aacttaaaga tgcggctgat cttccgggat tcatcccagt ccgctccgtc gaaatgatac
     4621 caatgccatt taaaatcact gtacgtgttt ccacggcccg gaaaacgaaa atccgtccac
     4681 gctttgattt gatattcctc cgaagtttcc tgatttctat tggccggatt gacttcgacg
     4741 gcagttacat cttctgttgc atcagcacca gccttatgat tcaaaaccac atctccgtat
     4801 acttggacgt tccgggaatg cagtgagccg atcgcatctt gaagctctga ttttgtgccg
     4861 tatttcgttc tgaccgtccc tttttgctgg aattctccta aatcatacaa atcataaggt
     4921 ccgtatccgt tatcggattg gctcaatcct ttgtatgcgg gaggaatcca gacggcagtg
     4981 attccgatat ccgataaatg ttccgcatca ttctgcaatc gtttccaatg ctggccgtcg
     5041 ttcggcgtat accattcaaa atactgcatc agcgtgccat ttacggctga tgtttttgta
     5101 atcggcaaac tgacaaataa cagcgtgcac ataagcacaa gtctgaacga aactgtccgc
     5161 tttcgttttt gaatcatgtt tcctctccct ctcattttct tatacaaatt atattttaca
     5221 tatcagtaaa ataataacac ccccctttat tcctattttt tacacagcgg acagtctgga
     5281 cagcataaaa aataccctgt ctgatgacag acaaggtatt tttatggtct tcttcttttc
     5341 tcaaacaatc gatccacttc ttcagccaaa tcatcagtca tcaaaggctc aatgttttca
     5401 gccagtcttt tcgtatgtgc ggggcttgcc ccgcttgttg atactgatac ttgaatgcgc
     5461 cctttgcgga tgatcttcgg catatatacg ctgccttgtt cagccttgct tacacagttg
     5521 accagctgcg tttcagaagc gtttgcggct atctcctgat tcacgcctcg gtcatttgtc
     5581 gcggcaataa tgaaaaaagc gggcttgaga tccaggagaa caaaaacgat tttttgagga
     5641 aagttataaa ttattttccg aacgatatgg caagcaaaat attgcttatg caacagttca
     5701 taatgatgag caaacccctc acatgcattt aggtgttgtg cctatgcgtg atggaaaact
     5761 gcaaggaaaa aatgtgttta atcgtcaaga actgttatgg ctacaagata aattccccga
     5821 gcatatgaaa aaacagggtt ttgagttgaa gcgtggtgaa cgtggctctg accgtaaaca
     5881 tattgagaca gctaaattta aaaaacaaac tttggaaaaa gagattgatt ttctagaaaa
     5941 aaatttagca gttaaaaaag atgaatggac tgcttatagc gataaagtta aatcagattt
     6001 agaagtacca gcgaaacgac acatgaaaag tgttgaagtg ccaacgggtg aaaagtccat
     6061 gtttggtttg ggaaaagaaa taatgaaaac agaaaagaaa ccaaccaaaa atgttgttat
     6121 atcggagcgt gattataaaa acttagtgac tgctcgcgag agataacgat aggttaaaac
     6181 agcatgttag aaatctcatg agtactgata tggcgagaga atataaaaaa ttaagtaaag
     6241 aacatgggca agttaaagaa aaatatagtg gtcttgtaga gcgatttaat gaaaatgtaa
     6301 atgattataa tgagttgctt gaagaaaaca agtctttaaa gtctaaaata agcgatttaa
     6361 agcgtgatgt gagtttaatc tatgaaagca ctaaggaatt ccttaaggaa cgtacagacg
     6421 gcttaaaagc ctttaaaaac gtttttaagg ggtttgtaga caaggtaaag gataaaacag
     6481 cacaattcca agaaaaacac gatttagaac ctaaaaagaa cgaatttgaa ctaactcata
     6541 accgagaggt aaaaaaagaa cgaagtcgag atcagggaat gagtttataa aataaaaaaa
     6601 gcacctgaaa aggtgtcttt ttttgatggt tttgaacttg ttctttctta tcttgataca
     6661 tatagaaata acgtcatttt tattttagtt gctgaaaggt gcgttgaagt gttggtatgt
     6721 atgtgtttta aagtattgaa aacccttaaa attggttgca cagaaaaacc ccatctgtta
     6781 aagttataag tgactaaaca aataactaaa tag
//
