LOCUS       NZ_CP111002             8802 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain J53-p133 plasmid p133-3, complete sequence.
ACCESSION   NZ_CP111002
VERSION     NZ_CP111002.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31669048
            Assembly: GCF_026247825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Evolution and transfer of virulence plasmids of hypervirulent and
            carbapenem resistant Klebsiella pneumoniae strains in hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2022) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Shanghai 272000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:37:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,924
            CDSs (total)                      :: 4,805
            Genes (coding)                    :: 4,560
            CDSs (with protein)               :: 4,560
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 73 of 245
            Pseudo Genes (incomplete)         :: 181 of 245
            Pseudo Genes (internal stop)      :: 45 of 245
            Pseudo Genes (multiple problems)  :: 53 of 245
            Pseudo Genes (short protein)      :: 4 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP111002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="J53-p133"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p133-3"
                     /geo_loc_name="China: Shandong"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2022-06-30"
                     /collected_by="Dongxing Tian"
     gene            1..246
                     /locus_tag="ORI88_RS24430"
                     /old_locus_tag="ORI88_24430"
     CDS             1..246
                     /locus_tag="ORI88_RS24430"
                     /old_locus_tag="ORI88_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            552..815
                     /locus_tag="ORI88_RS24435"
                     /old_locus_tag="ORI88_24435"
     CDS             552..815
                     /locus_tag="ORI88_RS24435"
                     /old_locus_tag="ORI88_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            805..1104
                     /locus_tag="ORI88_RS24440"
                     /old_locus_tag="ORI88_24440"
     CDS             805..1104
                     /locus_tag="ORI88_RS24440"
                     /old_locus_tag="ORI88_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1166..1543)
                     /locus_tag="ORI88_RS24445"
                     /old_locus_tag="ORI88_24445"
     CDS             complement(1166..1543)
                     /locus_tag="ORI88_RS24445"
                     /old_locus_tag="ORI88_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            1612..1791
                     /locus_tag="ORI88_RS24450"
                     /old_locus_tag="ORI88_24450"
     CDS             1612..1791
                     /locus_tag="ORI88_RS24450"
                     /old_locus_tag="ORI88_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1822..2085)
                     /locus_tag="ORI88_RS24455"
                     /old_locus_tag="ORI88_24455"
     CDS             complement(1822..2085)
                     /locus_tag="ORI88_RS24455"
                     /old_locus_tag="ORI88_24455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(2114..2845)
                     /gene="mobC"
                     /locus_tag="ORI88_RS24460"
                     /old_locus_tag="ORI88_24460"
     CDS             complement(2114..2845)
                     /gene="mobC"
                     /locus_tag="ORI88_RS24460"
                     /old_locus_tag="ORI88_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            complement(2845..4806)
                     /locus_tag="ORI88_RS24465"
                     /old_locus_tag="ORI88_24465"
     CDS             complement(2845..4806)
                     /locus_tag="ORI88_RS24465"
                     /old_locus_tag="ORI88_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6290..6439)
                     /locus_tag="ORI88_RS24470"
                     /old_locus_tag="ORI88_24470"
     CDS             complement(6290..6439)
                     /locus_tag="ORI88_RS24470"
                     /old_locus_tag="ORI88_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6524..6781)
                     /locus_tag="ORI88_RS24475"
                     /old_locus_tag="ORI88_24475"
     CDS             complement(6524..6781)
                     /locus_tag="ORI88_RS24475"
                     /old_locus_tag="ORI88_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6791..8476)
                     /locus_tag="ORI88_RS24480"
                     /old_locus_tag="ORI88_24480"
     CDS             complement(6791..8476)
                     /locus_tag="ORI88_RS24480"
                     /old_locus_tag="ORI88_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_266051493.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQDADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             1791..8802
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5609..6187
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2118..2845)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct
       61 caggcactgg ccatgggcct gaaagggaac aaaacgctgc caaaggcggt aaaacaacag
      121 gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa
      181 atgcgtcggg agaaatacga ggctacggaa accaccttca cctggcagcc acagcgccgc
      241 cggtaagggg tatttaccat aacgtcgcat tacgcgcacc gctcagacgg tctgagggca
      301 tttccggcgg ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg
      361 cccggtaaac tttatcctaa tgcatgccta tgcacgcaac tgcatggcgt gcagtttgac
      421 gtaaaatggg tgccgtaagc ccttttttac cagctttcgt cttcatcagc ctgactgaca
      481 cgagcatatt ctttcagacc gccccctgct aaacttcttc atatcacccc attaatccgg
      541 gagtctccgc aatgagcaac actacacact acgaaaacgc caacttcctg cgggaactgg
      601 ctgaaaacct gccccgcata ctgccggaaa gtggcccgga caaagctgcg ttgttgcaat
      661 gcctggctaa cgaagaactg gcccaggcag agtatgaaga tcaggttcgc gccaaagtaa
      721 ccgccgcccg tgctgacacg cgtccgggca tgaccacgga acaactccgc cagcgcctgc
      781 atggccgtta tcaggaactg cgcgatgccg tatgaggtgg aatggaaaca gggagccatc
      841 gaggatgtaa cgacgctgtt tgactatatc gcggaaaatt catccctgtg ggatgcgcag
      901 aacgttaccg atcgcgttct cactgcggcg gacaaactgg ccgacttccc gcgcctctat
      961 gaagtggact cacgctatgg cgaggacgtt cgccggatca gtctgatggg acagcatgtc
     1021 ctgtatgatg ttgatgacca gaccaggaaa gtccgggttc tggccgtggt tggccagcgc
     1081 cagaatcccc ataccgttcg ctgataaccg gtgacggggg cgcaattcat tgcgtggccg
     1141 tcgccggttg gacaagcaca gcgcgtcagc gttttcccca ctgccaccac cggcgcggtg
     1201 ttccggccgg tgtctctgct gtctccggtt tgtgttccag cagtctcatg gcctgcattt
     1261 cctgccgcag tccggccacc tcctccctga gtgcttttat ctccgcgatc aacgcgtcga
     1321 aggccgtttt ttcagtctca tccggcagtc tcaacccgtc ccgctcgtga gtctcaccct
     1381 gtcttaactc accgtacacc cggataagct cgctggtatc catcacccga ccaccatcag
     1441 cttccgtacg gtatgatacg cggcctttcg ccatatcccg atacagggaa ctccgggctt
     1501 tccccgtcag ttccgtcgcc tgtctcaccg tcacccacgc cattgtctca ccccgctatt
     1561 tttgagacag tatacccgac agacgcagac cgtgcccgga aggacagaaa catgacgaag
     1621 caggaaaaaa ccgccctcaa catggcgaaa ttcattaagg accagtcgct gctgttgctc
     1681 gagaaactga acgagctgga tatggatacc ccggccgaca tgtgcgaacg gctgcatgag
     1741 gatgcagaaa agctctggct ggcaatgcgg gaccatctcg gggaggaata acagcaccgg
     1801 ccgagccggt gctgtccggc attatttcat caggcggcgc atacagagga gtattccccc
     1861 gacaagaatg gccgccgtgc ctccctgccc gaaatgcaga aacagccagt cacggatctc
     1921 cgggttcctc gccagaaact gcacaacccc gtagagcagc agaagcaccg gcagtacgcg
     1981 ccaaagcagt ttcagcaggt ttccggccag aacgaccagc gtcccggcca tgacagcgac
     2041 catcaacagg gcttcacgcc ccacggcaac aacacgattt gtcataaaaa cctctctgag
     2101 tgaatgtata agctcagtcc atcatcatga atttaaagca cgcaaacagc cgctcccggt
     2161 ccagctccac cgttttgcgg gcctcgccgg tctgcgggtt cacgatttca tccaccgcca
     2221 gaatagcccg ttgcaccctg acctgcgtcg cccggtccgg gcaaatgtac cagatttcat
     2281 cgatgccgtt cgcccggcgg ttaaacagat acccggacac cacctcctga tagcgcctgc
     2341 ggctcttcac ggtgcgttcc acctcaaacg ccaccgttct gccgtccagc tccgccagcg
     2401 cgtccggcac cttcatccct ttcagggcaa tacggtgcag gtaccgccag ccggtggccc
     2461 cccgggcctc catggcgagg cgcagggact gcacggccag ccggtgcggc agctgcgccg
     2521 ccgacacccg cgacggctga aacggaataa tgtccgtcag cggctcgtcc tcgtcgaacg
     2581 agaacgccac cccgtgcggc gtcagccccc acacgctgac ctgacggccg tcaaccgggt
     2641 atttcgcccg gaccagcagc cctttcgcca ccaggctgtc caggaagcgg taaaccggct
     2701 gtaccgccgc aatccccagc cgttgcgcaa ccagctccgc cgtggaacac agttcttccc
     2761 gcagccagcg cagggtcagc cgtgttttct ccgcgttccg tgcctgccgc ttcgcgtggc
     2821 tgacgttgta tcgctccagt gccatcagta cagccccgca aaatcatcat cacccggccg
     2881 ctgccgcccg gcctccgact ggtactccgc cagcgggtcc gttttcgcca gcacagggaa
     2941 ggttttcagc ggaatttccc ggcgatcaac acgcagcggg ctgctgaacg ccagccgcgc
     3001 cacgcccagc ccggtcatca ccgcgaaccc gtcaggcaga tgttgcaggg tgttcacgtc
     3061 aaacagcggc cgcgcctcct tctggatgaa gctgtccccg ctgatatgct ccacgttccc
     3121 cgcctcccgg ctggcccggc ggcgctccac gtccacccgg atctccccgg tctgtccggc
     3181 cgcccactga gcggactcct ggctggccgc ccggtagaac cagcggatcg gcgtgttatc
     3241 cagtaccgtg gttttcacaa aatcagcggg cagatcctgc ccgcactggc cgaagtcgcc
     3301 cagcgactgg tgcgcgagca gcaggttgca gtcccggctg cgcagcgtcc ccagcgcgtt
     3361 cagcacgtac ttcgacagca gatatttaat ctcatcgagc atgatgctcg cgtgcggcca
     3421 cctccggaac tcgtcccgcg caatgatgat ttgcgcgcag cgggcaaaca gcatcttctg
     3481 cacccggatc accgcctcat cgtccatcga gccgatgacg tacaggcagc cgccgccgtt
     3541 gattatcccc gccacgtcga tgccgctgtc cgtctgaagg acgctcaggt cggccacgtt
     3601 ctccagcttg gtgatcagcc ccttcacgtc ctttttcagg gcctccggca gtgcgtacgc
     3661 cgccgccagc acctgctgaa tatttgcccc ctgcggaaac tgctccgcaa tcagcttcgc
     3721 cgctttctgc tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcac tcaggttaaa
     3781 tccggcaacc agaagattct tcagggcgta ctggtcgaga tcgcggaaca gattcagctg
     3841 cgccggtttc cccggccgca gatccagcag ggtgaagtcc gggcagtgcg cccggaagac
     3901 gtgcggcgcc cacgcgtccc ctttcgggtc gaagtacacc accgcgtccc cgaaatcgcg
     3961 tacgcagcga atgagcgcgt tggtggccat cacgctcttg ccgctcccgg gcaggccgag
     4021 gatctgaatg ttggtcttgc gccaggtatc acgcgggacc agcaccggct ggccctgctc
     4081 atccttaccc agaaacaccg cctgctgttt ccgggcctgc tccgtggaaa aatcggccag
     4141 cgcgttaacc ggtaccggct ccccgaaatg caggtggcgg acgtcggtca tttctccggt
     4201 ccgctggctg ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc
     4261 cgcaaaatgc ccgtcaagca gcaggaacgc cagcagccgc gccaggagga cgcctgcgac
     4321 aagacctccg gcacaccagg caatcccgaa caggttagcc ggctgcagca ggtaataatc
     4381 tgcgttcgcg tagaggtccg gacgcaggac ggtttctccc cagctctcga taaacacggc
     4441 gacaaccagc ccccccagaa gggccagacc ggcagcgccg gtcagccagt atgcgtagtt
     4501 ccagacatgt cgccgtgccc tgaccatccg cccgggcgaa tccagcgagg ccagcaccga
     4561 aaatggcgca gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgctcctt
     4621 atgactcagc cccacgaatc gcatccgccc ggcccagagt tctgccagac cgaaaaacag
     4681 gccgataacc agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca
     4741 cgggtaaatc tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt
     4801 aaacatgcaa tctctctcct tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct
     4861 gcacaagccg cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg
     4921 ttcagctttc accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg
     4981 gaggaacaac gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt
     5041 ttccggtgtt ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat
     5101 caggagcagc tttgctgctt cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc
     5161 aggcgtgagc ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacag
     5221 agccagaaag aggggaaaag accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct
     5281 tttcccctga agtcagccac gccccagcgg ggatgacttg catttctccg caaggagaaa
     5341 aaagcaacag agataggtta acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat
     5401 cctgataatt gatcgtcagg gaatagaaag acttaatcag gggacaatat cccacatcag
     5461 cgcgtccggt gaccgtcttg caggtgccgg ggaaattgcg cctcctcgct cactcggtcg
     5521 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
     5581 ttccgtggat aagcagggga ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg
     5641 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt tttcggtgca
     5701 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
     5761 ttaaagatcc ccaccgtttc cggtaggtcg ctccctcttg cgctctcctg ttccgaccct
     5821 gccgtttacc ggatacctgt ccgccgttct cccttgcggg aagtgtggcg ctttctcata
     5881 gctcacacac tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca
     5941 agaactcccc gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa
     6001 cccgggaaag cacgcaaaac gccactggca gcagccattg gtaactggga gttcgcagag
     6061 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
     6121 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
     6181 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
     6241 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     6301 cgatccccgt cagttcagag gaggacgatg gtgccaccgt tccgccctga acatcccgga
     6361 tataatttgc ctgacatgcg gccagaagaa agccagcaac aatcaatata aaaagaaaaa
     6421 tagtctttac ctttttcata tactttccct gtatttaaag actccggcat tcatccctga
     6481 acaccggaag tcagtatttc aaatcatcct tgataatttt ttgttaccag ttaccgtccc
     6541 gataatcaaa agctatataa caattatcca catcaggttt tatttgattt ttaaaaaatg
     6601 gctgtagtaa aggaacccat tctttcttca aatcaaactc accattatta atattatcct
     6661 ttaaaggcat tcccaagctt tcaatgacag aaccatcgtc accaaagtct ttagaatact
     6721 cttccccttt aaactcttca gttttcttat caaaccatgt caaacgtaat ttaagcccca
     6781 tatttacctc tcaaagatat tttttaatgt tccgttttgg atctggccct ttaacctgct
     6841 tacccgtttt gggatcaaat gcgcctaggt gttcgccatc actggcacga tacccttcaa
     6901 gctcaccgtg ctgggaatcc cattcataaa ttttacgttt tttatcgcca taccatcggg
     6961 cccgcttacc accaccacct tgtttaggtg ttttaggatc acctcgcttt aactctccaa
     7021 gtccctttat gtcttctgtt ttaggtactg gatgataatc atggccgtag tctttagtcc
     7081 ccttgcgagg cttgttcttt tcctcattta acttattttc tgcatccttc gcttttttct
     7141 ccttttgttt ccggctctcc atcgccccgg taagtgcagc cttatctctg gataaggatt
     7201 cattcattgc gtttaaagat gcctgagccg cttttaattt ctcattctga ttctgaatat
     7261 ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga ggcgggttat
     7321 aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg gcgtcctgaa
     7381 cagcagccct tgcaccgggg agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg
     7441 caatattttt atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc
     7501 cctcttccgg atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct
     7561 gttttacctg tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg
     7621 tctctttcgg gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg
     7681 gacgtgaatc acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac
     7741 ctttcggtac gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg
     7801 gacgttttgt cggtttcgca tccaccacag ggacactcat tggccgacct gcgacaaccg
     7861 caatatgctg ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg
     7921 cctggtccag tggtaaagta cttgccggag tctccgtcac cgcatcggcc ggtaatgacg
     7981 tcataatttt tgacatcatt ttcggatcat cttttgcaat ctcagaaggc agcacaccgt
     8041 agatcgcaat cccccacaga ccaaacttaa acggcccctt cagggcagcc agcacatcag
     8101 caacggcggc cgacaacgca tcgccggaaa ctgataaagc cagtccttca gcccccggag
     8161 tagccagggc aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc
     8221 taccgctatg attgccgctg ttaccaccat taccaccggg tttactcccg ccgttaccaa
     8281 actcccccgg attatagtta tgaatatctc cattcggcgt atgcgtcgta ccccagcccg
     8341 gcccgctgtt tggatccgta ccaccacctg atgaagggcc acctttacca gacgtaccgt
     8401 tcacattccc actggcctga ccaccattat gtcccagacc tgaattaccc ggacctcgac
     8461 cgtctccacc actcatatcg ttttcctctt tgacatttaa aacaataaat taaagccaat
     8521 atactgtaca tatacacagt ataattatta ccttactcct cccgttttca ggtgtcaata
     8581 tatgagatta gttctcatct tcaggcacaa aaaagcgccc ggagccacct gtaagcagat
     8641 cgcgtctttt cccgaaaatt tctcatggca acaaatttta ttatttaaga cattcatcac
     8701 acttacattt cagatggtat agacacatct gaaagctaac tggcatgagg agaggacctg
     8761 cggttaaact gttcgggaaa cctgagacgc ccggaggcca ct
//
