LOCUS       NZ_CP110994             2461 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain XYEH3314 plasmid pYUXYEH3314-7, complete
            sequence.
ACCESSION   NZ_CP110994
VERSION     NZ_CP110994.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31720454
            Assembly: GCF_026248845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of E. coli XYEH3314
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2022) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:38:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,132
            CDSs (total)                      :: 5,014
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 76 of 246
            Pseudo Genes (incomplete)         :: 147 of 246
            Pseudo Genes (internal stop)      :: 52 of 246
            Pseudo Genes (multiple problems)  :: 28 of 246
            Pseudo Genes (short protein)      :: 4 of 246
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110994.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="XYEH3314"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pYUXYEH3314-7"
                     /geo_loc_name="China: Xuyi"
                     /lat_lon="33.01 N 118.54 E"
                     /collection_date="2021-06-25"
                     /collected_by="Guo-Zhuang Sun"
     gene            join(2432..2461,1..987)
                     /gene="mobV"
                     /locus_tag="ORI32_RS24935"
                     /old_locus_tag="ORI32_24910"
     CDS             join(2432..2461,1..987)
                     /gene="mobV"
                     /locus_tag="ORI32_RS24935"
                     /old_locus_tag="ORI32_24910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_089637218.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENHCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLENKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSQAVNDGFAGTVAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(1086..1316)
                     /locus_tag="ORI32_RS24940"
                     /old_locus_tag="ORI32_24915"
     CDS             complement(1086..1316)
                     /locus_tag="ORI32_RS24940"
                     /old_locus_tag="ORI32_24915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029488008.1"
                     /translation="MMNAEDKLFKKVDRLMLHDSLKKNEVLTVWERTFLSSTYSIYRRT
                     SGNEFSTKGLSPKQKSTAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     gene            complement(1303..1785)
                     /locus_tag="ORI32_RS24945"
                     /old_locus_tag="ORI32_24920"
     CDS             complement(1303..1785)
                     /locus_tag="ORI32_RS24945"
                     /old_locus_tag="ORI32_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202974839.1"
                     /translation="MNTKKISKLERHTYMDESGEIKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFLRAGRGELEANPFLFARGDWPSIYERRKKIELRISYDSNGRTVQTTVTDDER"
                     /name="hypothetical protein"
     Typing          2..984
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.951"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 cagaacgagg gggtaagcat ggcgaattac gcgataatgc gctgcaaaaa gctgacggga
       61 atgggcagtg tggccagcgc tttgcagcac tgctacaggg agcgtgagac gccgaatgct
      121 gacgctgaac ggacaccaga aaaccactgc tctgtgtctc agtcggcaga tgaggcgatg
      181 ggaaagctgc gggagctgtt gccggaaaaa cgccgtaaag atgcggtact ggctgttgaa
      241 tacgttatga cggcaagccc tgagtggtgg aacgaggcca caccgcggca gcaggcggag
      301 tttttcgccc gttctgagca atggctggag aacaaatacg ggaaagatcg cgttgtggcg
      361 gcggtggttc acagggacga ggccaccccg cacctgtctg cgttcgtggt gccattgacg
      421 caggatgggc ggctgtccgc gaaagagttt attggcgggc ggtcaaagat gcgtgacgat
      481 cagagcacct acgctgaatc ggtaaaaaaa ctgggtcttg agcgcggcat cgagggaagc
      541 cgtgccacgc atcagacagt tcagcactac tacgaatcga taaaccgtgg cacccgtagc
      601 caggtatcga tctcaccgga ggcgctggag ccgcgtgtac tgcggaaggg tattttcacc
      661 aaagacgtgg aagatcaggc agccatagcc aaacgtttaa gccaggcagt gaacgatggc
      721 tttgcaggaa ccgtcgctat ggcctctcag agcgcccaga atgcaaaaag agcgcgcgag
      781 ctacagaaga ctatggattc gcagcagaaa cgcctacaga gcgttacaga gccttttaag
      841 ggcctttcca gagagcagat gaccgaaatc ctgatgatgg ctcagcggtt taaacagcaa
      901 aaccaggaaa aggaaaaaca gcagcgtatt gaacgtgaaa aacagcgaca gacgcgatcg
      961 cgtggtatgg gtggtatgag tcgttagttt tgatgcgatg caggataggt ctgacgccgc
     1021 aaaacgaggt catttgcggc attccagaga tcaggttctg ccgccctgta taacgctgag
     1081 tccagctaat ccatcagatg gtaattgtat tttttcaaaa tagaaaaagc ggtgcttttt
     1141 tgttttggtg acaatccctt agttgaaaat tcattaccgc ttgttctgcg atatatgctg
     1201 tatgtactac ttaaaaaagt acgctcccaa acagtcaaaa cctcattctt ttttagagag
     1261 tcatgcagca ttaatctatc tacttttttg aatagcttat cttcagcgtt catcatccgt
     1321 taccgttgtt tgtactgtcc ttccattgga atcataactg attcttaact ctattttttt
     1381 tcttctttcg taaattgaag gccagtctcc ccgagcaaaa agaaacggat tggcttcaag
     1441 ctctccccgc cctgcccgga gaaagatttg tttatcaaca agggcagcga ttatgttttt
     1501 aaacgtctga tattcaatat gaaccctagg ttgtatggtc ttctcaaatc tagtttttga
     1561 tacagataca aagccatccc atccaatcag cattgccaac tgcaaaagca catcattttg
     1621 agattttgac aaaccatgaa tcatcgcaat gtctgcgatg taaagcttga cgtagggcgg
     1681 ttcagccggg atagaaaaag tctcgatctt agagtcttgt ttaatctctc cgctctcgtc
     1741 catgtatgta tgccgttcaa gcttagaaat cttttttgta ttcactctag ccccatgtat
     1801 cagcatgttt ttagtactca catttaagta ctaaagatgc cggtagtcaa gtaccttacc
     1861 taaagtacga ttagtacttg aggtgagtac gattagtact tgaggtgagt acgattagta
     1921 cttgaggtaa gtacgattag tacttgaggt gagtacgatt cttctctcta gcccttggtg
     1981 tgcgcgggtt cccgtatcta tatctatata ttaaggggga aaataaaggc tgaaaaacgc
     2041 catttttaaa catcgaaaaa cgagcttttt tgagtctgga gcgtacggat ttctatgtcc
     2101 ggggagtgga aattccgctt cctcgaacgg acagactcgc ggcgatgccg ctccctaatc
     2161 cgttcttgcg gcgagcggtt cctgctcttt caaaaacgat attcattgcc tgatttcgtc
     2221 aggggcgagc gaagcgagtg ccatttaccc ttgacgactc accggataaa acaggcttgg
     2281 cagtggtggg gggtggcgcg gagcgacgcc ttccacctgc cagactgccg ttgattttga
     2341 ccttctggca gcttgctgcc cgaacgattt tgcgtagcaa agtctagtga gtacactatc
     2401 atcctgatag tggcccgccg gaggcatcgc aatgcgatcc cccgactggc tggatgtacg
     2461 c
//
