LOCUS       NZ_CP110993             3174 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain XYEH3314 plasmid pYUXYEH3314-6, complete
            sequence.
ACCESSION   NZ_CP110993
VERSION     NZ_CP110993.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31720454
            Assembly: GCF_026248845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of E. coli XYEH3314
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2022) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:38:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,132
            CDSs (total)                      :: 5,014
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 76 of 246
            Pseudo Genes (incomplete)         :: 147 of 246
            Pseudo Genes (internal stop)      :: 52 of 246
            Pseudo Genes (multiple problems)  :: 28 of 246
            Pseudo Genes (short protein)      :: 4 of 246
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110993.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="XYEH3314"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pYUXYEH3314-6"
                     /geo_loc_name="China: Xuyi"
                     /lat_lon="33.01 N 118.54 E"
                     /collection_date="2021-06-25"
                     /collected_by="Guo-Zhuang Sun"
     gene            join(3161..3174,1..310)
                     /gene="mobA"
                     /locus_tag="ORI32_RS24910"
                     /old_locus_tag="ORI32_24885"
     CDS             join(3161..3174,1..310)
                     /gene="mobA"
                     /locus_tag="ORI32_RS24910"
                     /old_locus_tag="ORI32_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052584083.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQVIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(699..1127)
                     /locus_tag="ORI32_RS24915"
                     /old_locus_tag="ORI32_24890"
     CDS             complement(699..1127)
                     /locus_tag="ORI32_RS24915"
                     /old_locus_tag="ORI32_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            1285..1455
                     /locus_tag="ORI32_RS24920"
                     /old_locus_tag="ORI32_24895"
     CDS             1285..1455
                     /locus_tag="ORI32_RS24920"
                     /old_locus_tag="ORI32_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            1419..1847
                     /locus_tag="ORI32_RS24925"
                     /old_locus_tag="ORI32_24900"
     CDS             1419..1847
                     /locus_tag="ORI32_RS24925"
                     /old_locus_tag="ORI32_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2683..2877)
                     /locus_tag="ORI32_RS24930"
                     /old_locus_tag="ORI32_24905"
     CDS             complement(2683..2877)
                     /locus_tag="ORI32_RS24930"
                     /old_locus_tag="ORI32_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001485273.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2913..3152
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(2123..2648)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.03"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          3..307
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.039"
                     /coverage="95.0"
                     /evalue="7.78e-58"
ORIGIN
        1 tggaagtaat acgcgcaggc gggctatcag tcgccctgtt cgtctgacgg cagaagaaga
       61 ccaggtaatc agaaaaaggg ctgctgaatg cggcaagacc gtttccggtt ttttacgggc
      121 ggcagctctc ggtaagaaag ttaactcact gactgatgat cgggtactga aagaagttat
      181 gagactgggg gcgttacaga aaaaactctt tatcgacggc aagcgtgtcg gggacaggga
      241 gtatgcggag gtgctgatcg ctattacgga gtatcaccgt gccctgttat ccaggcttat
      301 ggcagattag cttcccggag agaaaactgt cgaaaactga cggtatgaac accgtaagct
      361 cccaaagtga tcaccattcg ctttcatgca tagctatgca gcgagctgaa aacgatcctg
      421 acgcatcctt cctgtttttc cggggtaaaa catctctttt tgcggtgtct gcgtcagaat
      481 cgcgttcagc gcgtttcagt ggtgcgtaca attaagggat tatggtaaat atatgagcta
      541 tgcgataact ttaactgtga agcgatgaac ccattacagg caaagccaat tactcctgac
      601 agtggtttag ccagaagcag ggctaccaag accaatgcaa taagtaatat atcgttttgc
      661 tatcgtgcat cgctcgcgct cagttccatt gtgctttttt aagctgtcgt ttttcttacg
      721 gtatataccg gttttttatg gcgtggtttc ttaacttgtt cagctactga tggacccatt
      781 gtatctaggt agtcaactag ctttgttaga tcataaatat tgcgaccacc atatcggcga
      841 tcactcttcg atgttggttt cttgtccaag agattagctt tttcaagatc attgatagct
      901 ctctgaacag tccgtacaga aacccccata cgtatggcta gactttccat tgacggatgc
      961 ggccactctt gcaaactcca ccagtgaacg atcaggttaa gtagtgtgtt aaaggccact
     1021 gaagttagct ttttctcgtt ttgtataaaa aacaatacgg taggcactgc tgtccagcca
     1081 agagacaaac cgccagcttt ccatttattc ttaacggagt aagtcattga ttttcctaag
     1141 ccccaaaata tttaaagtat atattatatg gtatattcat atgaataggg tgacactagc
     1201 gccattattg tgcaaccaaa aaagactact ctgaaaacga ggaaaagatt ttttcctgcc
     1261 tgaattagat acggagttag cgatatgaaa accgaacaac gtcatgatct tgttaaagat
     1321 attgaggttt ttggcgtatc cttgtctctg ttgatttcca gagcgaatga gaagtctgtt
     1381 acaatgccat ctggtctaag tcgggagcag agaagagcat gggcagcgga gcaggcgcgc
     1441 aaaatccaca attgaatatt gtctcattct ctgagacctt caacctttat tacacatcca
     1501 gatattctgc aaaaacactc gataaaatcg atgatttcat tgagcatttt gaaaaataca
     1561 atctctttgg cgatccttta aaaggatatc cagcttggac tggcaaagta tcgccatcgt
     1621 ggaaagtgcc tgatcattac gaaaacaaag aagctattga gaagtatgct agagctaaca
     1681 aattatggca tgctcattta ggcgatccgg tttttaaaga tacgtttcat gggaaataca
     1741 aggtttctga ctgggttatt catttccagc ggctgacacc gaaccatata aagcttttag
     1801 agcttggata ccatgaccca atgaagctac ctcaaaactt tgaatgatcg agcggcaggc
     1861 taaatgaaat cttgagattc attcagtctc gtcgtaatct cactattgta aaaacgaaaa
     1921 aaccaccctg gcaggtggtt tttcgaaggt tagttaatcc tggcagattc tctaaccgtg
     1981 gtaacagtct tgtgcgagac atgtcaccaa atactgtcct ttcagtgtag cctcagttag
     2041 gccgccactt caagaactct cgttacatct ctcgcacatc ctgcttacca gtggccgttg
     2101 ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2221 acaccgaacc gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2401 agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccaacggcg
     2461 cggcctttgt ccctggcttc ggctttttgc tcacatgttc tttccggcgt tatcccctga
     2521 ttctgtggat aaccgtatta ccgcctttga gtgagctgac accgctcgcc gcagtcgaac
     2581 gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgccttatgt gacattttct
     2641 ccttacgctc ctctatgccg ttctgcatcc tgtccggatg cgttatatcc cggtaagatt
     2701 ttccgcttca aagcgtgtct gtatgctgtt ctgaagttct tccgcgagtt cgtgcagctt
     2761 ctcacacatg gcggcctgtt cgtcagcatc aagtgcgtcc agtttttcga gcagcgtcag
     2821 gctctggctt tttatgaatc ctgccatgtt gagtacggtt tgctgctgct tgttcatctt
     2881 tctgttttct ccgttctttc tgtcatctgc gttgtgtgat tatatcgcgt accacttttc
     2941 gactgtttta ccgtcggtat tctgctgtct gactctttga cgggcatttc tgtcagacaa
     3001 cactgtcact gccaaaaaac tgccgtgcct ttgtcggtaa ttcgaacttg ctgacaggac
     3061 aggatgtgta attgttatac cgcgcataca tgcacgctat tacaattacc ctggtcaggg
     3121 ctttgccccg acacccatgt cagatacgga gccatgtttt atgacaaaac gaag
//
