LOCUS       NZ_CP110992             4064 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain XYEH3314 plasmid pYUXYEH3314-5, complete
            sequence.
ACCESSION   NZ_CP110992
VERSION     NZ_CP110992.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31720454
            Assembly: GCF_026248845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of E. coli XYEH3314
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2022) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:38:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,132
            CDSs (total)                      :: 5,014
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 76 of 246
            Pseudo Genes (incomplete)         :: 147 of 246
            Pseudo Genes (internal stop)      :: 52 of 246
            Pseudo Genes (multiple problems)  :: 28 of 246
            Pseudo Genes (short protein)      :: 4 of 246
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110992.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="XYEH3314"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pYUXYEH3314-5"
                     /geo_loc_name="China: Xuyi"
                     /lat_lon="33.01 N 118.54 E"
                     /collection_date="2021-06-25"
                     /collected_by="Guo-Zhuang Sun"
     gene            complement(join(3562..4064,1..199))
                     /locus_tag="ORI32_RS24895"
                     /old_locus_tag="ORI32_24870"
     CDS             complement(join(3562..4064,1..199))
                     /locus_tag="ORI32_RS24895"
                     /old_locus_tag="ORI32_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(733..987)
                     /gene="mobC"
                     /locus_tag="ORI32_RS24900"
                     /old_locus_tag="ORI32_24875"
     CDS             complement(733..987)
                     /gene="mobC"
                     /locus_tag="ORI32_RS24900"
                     /old_locus_tag="ORI32_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1166..3313
                     /gene="mobQ"
                     /locus_tag="ORI32_RS24905"
                     /old_locus_tag="ORI32_24880"
     CDS             1166..3313
                     /gene="mobQ"
                     /locus_tag="ORI32_RS24905"
                     /old_locus_tag="ORI32_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_053920598.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELKTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(989..1165)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1166..1524
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3563..4064)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1080..3310
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.856"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgccgtcc catgtcagga aaggcatcat gaaactctgc agccgtgatg gtcatggttt
       61 tttgttcatc cggcgaaggg gaatcttctc cggattttag gcggctgata cagaccagca
      121 ggaagcgctg ttcctgcaaa gtgagcttgt agcttgcctc gataagggcg tttgatttgt
      181 aagctatcag atctgacatt tcgcttgctc gttgctattg gtggcgtttt tttactgtac
      241 gccacttatc atatgggtgg aagttttttt tttgcaaccc cctttttctg tggattacac
      301 ccccaaaagc tccacctttc acccccaaaa gctccacctt tcacccccaa aagctccacc
      361 tttcaccccc aaaagctcca cttatcttgc cagaacatct ttaaaatcat ggtgttacaa
      421 gacctaaaaa ggttttaaaa aaaaagatct aaaaataaga tcttaaaaac ttaacggcga
      481 gcctgtggat aacttggggt gctgaaaaca aaaaaccaat aaaaacaatg cgttgcaata
      541 aagcagaatt gcaagtttcg aatacacccc ctccaaccct gtggaaaccc agtattcatg
      601 cgggttcagc gcatatcacc tgccgaaaaa ccgtgcgtat gtgctcaacc tggcgactga
      661 ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg gtcgctgggg ctgcggcgag
      721 cggacgatac aatcactgtt ctggcagcgc ctccagctcg ttaatcagcg gcagtatgcg
      781 cgtcacatca gcctgtcgtg tcacctctgc cttcaggaac tggagtaacc aggcacgcaa
      841 ctgcacctcc tccggctttc tgacctttgc cagcaatgca gcacccacaa caaccttctg
      901 cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt aatctcgcct cagcatcagc
      961 aatcttctgt tcgagcgttc tgctcatgat gtaactccgt gcgcgatgaa aaatcgcatt
     1021 ttagcgcgtc actggtagtt taaaaactga gctggcataa tgcacggcaa atcacgaagt
     1081 gcgcacttat acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg ctgaaagaaa
     1141 actacaaaaa ggcattacgg cagaaatggc gatttatcat ctcagcatga aaattatctc
     1201 cagaagcagc ggctacagcg ccgtagcttc cgctgcctat cgttccggtt cactgatgct
     1261 tgatgagcgt accggactga ctcacgacta cacccgcaaa agcggtgtgg ctgaggctgt
     1321 gattctgacc cccgccaccg cccctgcctg gtgtacgaac cgtgccgaac tctggaatgc
     1381 cgttgagaag gccgaacgcc ggaagaactc acaacttgcg cgggaaattg aactcgccat
     1441 cccccacgaa ctgccgcagg atgccgcacg ggaaaccgtt ctcgcctttg tcagagaaaa
     1501 ctttgtcagt cagggcatga ttgctgatgt ggcgttccat cacatggata aaacaaatcc
     1561 ccatgcgcac atcatgctga ccacgagggc tatcggacct gctgggttca gcggaaaggt
     1621 cagggactgg aacgaccgga cacatgccga gacgtggcgg gcatcatggg ctgaccatgc
     1681 aaacagggca ctggcgaacg ccggttatca ggaagagata gaccatcgtt catacgagcg
     1741 acaggggctt gagaaaacgc ctggtattca cctcggaaag tctgcctgtg cgatggaaac
     1801 acgcggtatc gaaacagaac gcggcgaaca gaatcgtctg attaacaggc ttaacctgga
     1861 aatacagatt tcccgcaccg aactcaggaa cggagggctg acagagcctg cccgccgtgt
     1921 ggctgacctt ctgaatatcg ggcttcctga caacgcaact tcgtacacac tacgtgacat
     1981 tatcgaagcg ttgccgaagg acagtaactc ggcatggaaa ctcaccagta atgccatgtc
     2041 gatgatggca gatatgcagg cgacgcgcag acgctgggaa gagctgaaga ccgaacgaaa
     2101 ggaagcgatg agccatgctg ccagcctgaa aaaatcatcg ccgttttcgt ccggtttttc
     2161 ccgtattccc ctgatgcaat gggcggcacc ggaataccgt agtgagcagg gaaaaatcca
     2221 gtctctgagc cagcaaatgg aaaaattacg acagcattac cggaaggtgg agaaacagga
     2281 cattcccgcg agtcagatgg catttgaaaa acagtggcag cagtgggggg aatccggcct
     2341 tgcagctctc agagcccagt tgcttaaacg tgaagaggaa ttgcgccgga aggaacagga
     2401 agatactgaa cgtagtcgcg cggagcagct caggaaaaat gacaatgccg ttatggattc
     2461 aggggtgctg agagcagtcg ttacggggta tggcgaggct cccatgcctg gtaacgggga
     2521 aatgacctgt taccttctgc ttcacaaccg gaacggagaa tatacgctct gggggaacga
     2581 gctggagaaa taccgcacca gaatctttga gagtgttgat ttaatgcgtg acaggagtgg
     2641 ttatatctgt gaccgaagcg aaatcggcca acgtccgcca ctgcaacgtg tttattcctc
     2701 tgcaaccttt gagcaactgc tgacgcaggt ttgccagaca tggccgcaac atacccgcaa
     2761 tctccgccag ccgaagacat ggccggagtc tttctgtctt ggagaagacc gccagccagc
     2821 catgccatca ccagcggcaa ggaaggtgga ttttatgcag ggacgtctgc caccgacgct
     2881 gatgccggtc atgagttctg tcgaccggga aacccgccag cttcagcttt tgctggtcat
     2941 gggcgtggat gactctctgg gcggtgttat ccggcttaat ggtacgctgt atcccgcgtt
     3001 tgccgttccc tcagccgata acaggcatct ggtcatcagt gcgctgaccg ataaggggtt
     3061 acggtatgca gggtatggtg ttgccgtaaa tcacgatgca gacagccaca tatcacctgc
     3121 accagaactg atgcagtttc atctgaaaac ccgtgaggca ccgctttttg ctgccgtcaa
     3181 cacaccagaa aaacagccgg atcatctgtt ccgctcgctg ggttttaatc gtacctggga
     3241 ggaatggcag cgcgaggaag atgcacgaac acacaccacc gagaggagac atgatcgcgg
     3301 ttggtcacag tgaatgtgaa caatattttg ttttgtttaa catcgtgtta tgttcgtcga
     3361 tgttcatcga cagggatgaa tgcaagaagc acactcacat aacgcaagca tagttttgtt
     3421 tttccgcccc ccaaaaattg gggggtcttt tttcgctgca tcagagcgtt tttgacgcaa
     3481 tttttcactt caatagtacc ttcgcactac taacacaaaa aaaacgcagc agggagcgtt
     3541 ttaggtgcct taaaatcgat tttagatcgt catcttgatc tgtttgtctt ccttgaagcg
     3601 aaaatgcagg gctacaaccg tgcgcccctt tttgatcgtc tcaacagtta caaccagatc
     3661 gcttttttcg ttcagttcgg cgatggctgg cttgattatc atcttattca agtctttgaa
     3721 ttgttgatat ttatcttcaa cggcaagcat ggttcggaaa tcatcgatag ctatcgttct
     3781 gtcaccggtg ctgcggaact gctggagcag ctcgtaaatg cggatgctgt aggtgctgga
     3841 aaggctggag acgttcttca cgactacccg cgtaaactgc cccttgagct gtgtcagata
     3901 cggcatgatg gcatcggaga aggtgatatc tactttcgcc tcacctttga aatactgcgc
     3961 ccggtactgc acccagcgga actcttctcg tttttcctcg ttcttgatga ttactgacct
     4021 gtcccagagg cgatctatcg cttccttgag cctagcttca gcgt
//
