LOCUS       NZ_CP110991            21237 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain XYEH3314 plasmid pYUXYEH3314-4, complete
            sequence.
ACCESSION   NZ_CP110991
VERSION     NZ_CP110991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31720454
            Assembly: GCF_026248845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21237)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of E. coli XYEH3314
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21237)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2022) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:38:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,132
            CDSs (total)                      :: 5,014
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 76 of 246
            Pseudo Genes (incomplete)         :: 147 of 246
            Pseudo Genes (internal stop)      :: 52 of 246
            Pseudo Genes (multiple problems)  :: 28 of 246
            Pseudo Genes (short protein)      :: 4 of 246
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="XYEH3314"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pYUXYEH3314-4"
                     /geo_loc_name="China: Xuyi"
                     /lat_lon="33.01 N 118.54 E"
                     /collection_date="2021-06-25"
                     /collected_by="Guo-Zhuang Sun"
     gene            20..>253
                     /locus_tag="ORI32_RS24760"
                     /old_locus_tag="ORI32_24735"
                     /pseudo=""
     CDS             20..>253
                     /locus_tag="ORI32_RS24760"
                     /old_locus_tag="ORI32_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /name="SMR family transporter"
     gene            317..1021
                     /locus_tag="ORI32_RS24765"
                     /old_locus_tag="ORI32_24740"
     CDS             317..1021
                     /locus_tag="ORI32_RS24765"
                     /old_locus_tag="ORI32_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <1075..1686
                     /locus_tag="ORI32_RS24770"
                     /old_locus_tag="ORI32_24745"
                     /pseudo=""
     CDS             <1075..1686
                     /locus_tag="ORI32_RS24770"
                     /old_locus_tag="ORI32_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            1717..2601
                     /locus_tag="ORI32_RS24775"
                     /old_locus_tag="ORI32_24750"
     CDS             1717..2601
                     /locus_tag="ORI32_RS24775"
                     /old_locus_tag="ORI32_24750"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="WP_021598067.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDADQW
                     RIPNDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLAPTG
                     FGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALSKGLP
                     FRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGIVQGGS
                     VSWQLGRQRGLER"
                     /name="DUF3363 domain-containing protein"
     gene            2818..4032
                     /gene="floR"
                     /locus_tag="ORI32_RS24780"
                     /old_locus_tag="ORI32_24755"
     ARG             2819..4029
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="99.75"
                     /coverage="100.0"
     gene            4060..4365
                     /locus_tag="ORI32_RS24785"
                     /old_locus_tag="ORI32_24760"
     CDS             4060..4365
                     /locus_tag="ORI32_RS24785"
                     /old_locus_tag="ORI32_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_001255015.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALGI
                     SQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
                     /name="LysR family transcriptional regulator"
     gene            4477..>4665
                     /locus_tag="ORI32_RS24790"
                     /old_locus_tag="ORI32_24765"
                     /pseudo=""
     CDS             4477..>4665
                     /locus_tag="ORI32_RS24790"
                     /old_locus_tag="ORI32_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4632..5831)
                     /gene="tet(A)"
                     /locus_tag="ORI32_RS24795"
                     /old_locus_tag="ORI32_24770"
     ARG             complement(4636..5831)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            5937..6587
                     /gene="tetR(A)"
                     /locus_tag="ORI32_RS24800"
                     /old_locus_tag="ORI32_24775"
     CDS             5937..6587
                     /gene="tetR(A)"
                     /locus_tag="ORI32_RS24800"
                     /old_locus_tag="ORI32_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_001493764.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(6619..6861)
                     /locus_tag="ORI32_RS24805"
                     /old_locus_tag="ORI32_24780"
     CDS             complement(6619..6861)
                     /locus_tag="ORI32_RS24805"
                     /old_locus_tag="ORI32_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(7167..8003)
                     /locus_tag="ORI32_RS24810"
                     /old_locus_tag="ORI32_24785"
     CDS             complement(7167..8003)
                     /locus_tag="ORI32_RS24810"
                     /old_locus_tag="ORI32_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(8004..8422)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(8007..8806)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(8867..9682)
                     /gene="sul2"
                     /locus_tag="ORI32_RS24820"
                     /old_locus_tag="ORI32_24795"
     ARG             complement(8871..9682)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9990..10841)
                     /gene="repC"
                     /locus_tag="ORI32_RS24825"
                     /old_locus_tag="ORI32_24800"
     CDS             complement(9990..10841)
                     /gene="repC"
                     /locus_tag="ORI32_RS24825"
                     /old_locus_tag="ORI32_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(10828..>11547)
                     /locus_tag="ORI32_RS24830"
                     /old_locus_tag="ORI32_24805"
                     /pseudo=""
     CDS             complement(10828..>11547)
                     /locus_tag="ORI32_RS24830"
                     /old_locus_tag="ORI32_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /name="helicase RepA family protein"
     gene            complement(11597..12301)
                     /locus_tag="ORI32_RS24835"
                     /old_locus_tag="ORI32_24810"
     CDS             complement(11597..12301)
                     /locus_tag="ORI32_RS24835"
                     /old_locus_tag="ORI32_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<12529..>12810)
                     /locus_tag="ORI32_RS24840"
                     /old_locus_tag="ORI32_24815"
                     /pseudo=""
     CDS             complement(<12529..>12810)
                     /locus_tag="ORI32_RS24840"
                     /old_locus_tag="ORI32_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(12891..13595)
                     /locus_tag="ORI32_RS24845"
                     /old_locus_tag="ORI32_24820"
     CDS             complement(12891..13595)
                     /locus_tag="ORI32_RS24845"
                     /old_locus_tag="ORI32_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<13660..>15012)
                     /locus_tag="ORI32_RS24850"
                     /old_locus_tag="ORI32_24825"
                     /pseudo=""
     CDS             complement(<13660..>15012)
                     /locus_tag="ORI32_RS24850"
                     /old_locus_tag="ORI32_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <15014..15358
                     /locus_tag="ORI32_RS24855"
                     /old_locus_tag="ORI32_24830"
                     /pseudo=""
     CDS             <15014..15358
                     /locus_tag="ORI32_RS24855"
                     /old_locus_tag="ORI32_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /name="WbuC family cupin fold metalloprotein"
     gene            complement(15405..16280)
                     /locus_tag="ORI32_RS24860"
                     /old_locus_tag="ORI32_24835"
     CDS             complement(15405..16280)
                     /locus_tag="ORI32_RS24860"
                     /old_locus_tag="ORI32_24835"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /protein_id="WP_015387340.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSG
                     GRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKKSD
                     LVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETF
                     RLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAA
                     SIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLA
                     SAAKIVTDGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-55"
     gene            complement(16536..>16823)
                     /locus_tag="ORI32_RS24865"
                     /old_locus_tag="ORI32_24840"
                     /pseudo=""
     CDS             complement(16536..>16823)
                     /locus_tag="ORI32_RS24865"
                     /old_locus_tag="ORI32_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(16878..17582)
                     /locus_tag="ORI32_RS24870"
                     /old_locus_tag="ORI32_24845"
     CDS             complement(16878..17582)
                     /locus_tag="ORI32_RS24870"
                     /old_locus_tag="ORI32_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(18064..19077)
                     /gene="intI1"
                     /locus_tag="ORI32_RS24875"
                     /old_locus_tag="ORI32_24850"
     CDS             complement(18064..19077)
                     /gene="intI1"
                     /locus_tag="ORI32_RS24875"
                     /old_locus_tag="ORI32_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_000845048.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            19369..19923
                     /locus_tag="ORI32_RS24880"
                     /old_locus_tag="ORI32_24855"
     CDS             19369..19923
                     /locus_tag="ORI32_RS24880"
                     /old_locus_tag="ORI32_24855"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /protein_id="WP_063840321.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGRWEEETDPGVRGIDQLLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     YGPAVYMVQTRQAFERTRSDA"
                     /name="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
     gene            20020..20472
                     /locus_tag="ORI32_RS24885"
                     /old_locus_tag="ORI32_24860"
     CDS             20020..20472
                     /locus_tag="ORI32_RS24885"
                     /old_locus_tag="ORI32_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase Arr-3"
                     /protein_id="WP_001749986.1"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFEDG
                     RILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKRFPGNPT
                     QSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
                     /name="NAD(+)--rifampin ADP-ribosyltransferase Arr-3"
     ARG             20855..21078
                     /name="dfrA27"
                     /gene="dfrA27"
                     /gene_name="trimethoprim resistant dihydrofolate reductase
                     dfr"
                     /tool="rgi"
                     /drug_class="diaminopyrimidine antibiotic"
                     /identity="100.0"
                     /coverage="47.13"
     ARG             20606..21075
                     /name="dfrA27"
                     /gene="dfrA27"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA27"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             20021..20469
                     /name="arr-3"
                     /gene="arr-3"
                     /gene_name="NAD(+)--rifampin ADP-ribosyltransferase Arr-3"
                     /tool="amrfinderplus"
                     /drug_class="RIFAMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7171..8003)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(15409..16280)
                     /name="blaCTX-M-55"
                     /gene="blaCTX-M-55"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             19370..19920
                     /name="aac(6')-Ib-cr5"
                     /gene="aac(6')-Ib-cr5"
                     /gene_name="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE/QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             21..280
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="52.78"
                     /coverage="80.37"
     ARG             19325..19923
                     /name="AAC(6')-Ib-cr6"
                     /gene="AAC(6')-Ib-cr6"
                     /gene_name="AAC(6'); AAC(6')-Ib-cr"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic"
                     /identity="98.99"
                     /coverage="100.0"
     Typing          10823..11706
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga
       61 agtaatcgca acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc
      121 cgttgtcata atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat
      181 ccctgtcggt gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat
      241 tgcctggttg cttggcactg ttgcaaatag tcggtggtga taaacttatc atcccctttt
      301 gctgatggag ctgcacatga acccattcaa aggccggcat tttcagcgtg acatcattct
      361 gtgggccgta cgctggtact gcaaatacgg catcagttac cgtgagctgc aggagatgct
      421 ggctgaacgc ggagtgaatg tcgatcactc cacgatttac cgctgggttc agcgttatgc
      481 gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac ccttccgatc tttgcccgtg
      541 gcacatggat gaaacctacg tgaaggtcaa tggccgctgg gcgtatctgt accgggccgt
      601 cgacagccgg ggccgcactg tcgattttta tctctcctcc cgtcgtaaca gcaaagctgc
      661 ataccggttt ctgggtaaaa tcctcaacaa cgtgaagaag tggcagatcc cacgattcat
      721 caacacggat aaagcgcccg cctatggtcg cgcgcttgct ctgctcaaac gcgaaggccg
      781 gtgcccgtct gacgttgaac accgacagat taagtaccgg aacaacgtga ttgaatgcga
      841 tcatggcaaa ctgaaacgga taatcaacgc cacgctggga tttaaatcca tgaagacggc
      901 ttacgccacc atcaaaggta ttgaggtgat gcgtgcacta cgcaaaggcc aggcctcagc
      961 attttattat ggtgatcccc tgggcgaaat gcgcctggta agcagagttt ttgaaatgta
     1021 aggcctttga ataagacaaa aggctgcctc atcgctaact ttgcaacagt gcccggtgac
     1081 gggattggca aggtagccgg gtccagcctg cacgccggcg tggcggccag ggccgatgaa
     1141 cgcaagaagc tcgaacggct gtgccggtac atcagccgcc cggcggtatc cgagaagcgg
     1201 ctgtcgttaa cacgaggcgg caacgtgcgc taccagctca agacgccgta ccgggacggc
     1261 accacgcacg tcattttcga accattggat ttcattgcaa ggctggccgc cctggtaccg
     1321 aagcccagag tcaacctaac ccgcttccac ggggtgttcg cacccaacag tcggcaccgg
     1381 gcgttggtca cgccggcaaa acggggcagg ggcaacaagg tcagggtggc tgatgaaccg
     1441 gcaacaccag cacaacggcg agcgtcgatg acatgggcgc aacggctcaa gcgtgttttc
     1501 aatatcgaca tcgagacctg cagcggctgc ggcggcgcca tgaaagtcat cgcctgcatt
     1561 gaagacccta tagtgatcaa gcagatcctt gatcacctga agcacaaagc cgaaaccagc
     1621 gggaccaggg cgttacccga aagccgggcg ccaccggctg agctgctcct gggtctgttt
     1681 gactgacgag cctgaaggcc aacgatacca atcaaaatgc tgcgttcaca gcgccgcggc
     1741 agggatccgc cgtgctggtt gtcggaaaag gagccgctag tgggaaagag gagggtaaat
     1801 tttcagcgtt gctggctccc cgtcagccgg attgggttgc atcgcagggg tgtcgaaaga
     1861 gtcaactgcg gtccaaagct gttggacttg ggtgaaaagg gcgtttattc ttcctatacg
     1921 tgccgcgttc caggcggcga ctatgagggc tatgtcgatg cccatgtgcg ccggctggag
     1981 gcgctacgcc gggccggtat cgtcgagcgg atcgacgccg accaatggcg catccccaat
     2041 gatctggtca gccgtgccgc cgcccatgac gccggccgag acagtcaggc cagcgttcgc
     2101 gtcctttccc cggtcgatct gaacaaacag atcggatcgg acggcgcgac ctggctggac
     2161 cggcggctga tccacggcga gacggccgac cttgcgccaa ccggcttcgg gcaacaagtc
     2221 cgcgaagcca tggaccagcg ccgcgagcac catatcgaac agggcgacgc cacccgcagc
     2281 cgggacagcc gcgtcttcta ccggcgcaac cttctcgcca tcctgcggga gcgcgaggta
     2341 gccggcgtcg gatcggatat ggctttgagt aagggcctgc cgttccgcgc cgccacggac
     2401 ggcgagagcg tcagcggcaa gtttaccgga accgtgcatc tatcgagcgg caagttcgcc
     2461 gtggtcgaga aatcccatga gttcaccctt gtcccgtggc ggccgatcat cgaccgccaa
     2521 ctcggccgcg aggttatggg catcgtgcag ggcgggtcgg tgtcgtggca gttagggcgg
     2581 cagagggggc tggaacgctg agtgcgccca tgccgcattg cgaagcaaaa gataatcgga
     2641 taaaatgtag caattcatat tcgtaagcgt ggagtaatca gatgggaaat tccaagtcag
     2701 cagacaagta agccgcaaca accagtattg ttgttgcggc gctctgtaag gctagtctca
     2761 tctgattgct gacgagcaga cgtcgcccgg tattccttaa tcgagggttg attcgtcatg
     2821 accaccacac gccccgcgtg ggcctatacg ctgccggcag cactgctgct gatggctcct
     2881 ttcgacatcc tcgcttcact ggcgatggat atttatctcc ctgtcgttcc agcgatgccc
     2941 ggcatcctga acacgacgcc cgctatgatc caactcacgt tgagcctcta tatggtgatg
     3001 ctcggcgtgg gccaggtgat ttttggtccg ctctcagaca gaatcgggcg acggccaatt
     3061 ctacttgcgg gcgcaacggc tttcgtcatt gcgtctctgg gagcagcttg gtcttcaact
     3121 gcaccggcct ttgtcgcttt ccgtctactt caagcagtgg gcgcgtcggc catgctggtg
     3181 gcgacgttcg cgacggttcg cgacgtttat gccaaccgtc ctgagggtgt cgtcatctac
     3241 ggccttttca gttcgatgct ggcgttcgtg cctgcgctcg gccctatcgc cggaacattg
     3301 atcggcgagt tcttgggatg gcaggcgata ttcattactt tggctatact ggcgatgctc
     3361 gcactcctaa atgcgggttt caggtggcac gaaacccgcc ctctggatca agtcaagacg
     3421 cgccgatctg tcttgccgat cttcgcgagt ccggcttttt gggtttacac tgtcggcttt
     3481 agcgccggta tgggcaccta cttcgtcttc ttctcgacgg ctccccgtgt gctcataggc
     3541 caagcggaat attccgagat cggattcagc tttgccttcg ccactgtcgc gcttgtaatg
     3601 atcgtgacaa cccgtttcgc gaagtccttt gtcgccagat ggggcatcgc aggatgcgtg
     3661 gcgcgtggga tggcgttgct tgtttgcgga gcggtcctgt tggggatcgg cgaactttac
     3721 ggctcgccgt cattcctcac cttcatccta ccgatgtggg ttgtcgcggt cggtattgtc
     3781 ttcacggtgt ccgttaccgc gaacggcgct ttggcagagt tcgacgacat cgcgggatca
     3841 gcggtcgcgt tctacttctg cgttcaaagc ctgatagtca gcattgtcgg gacattggcg
     3901 gtggcacttt taaacggtga cacagcgtgg cccgtgatct gttacgccac ggcgatggcg
     3961 gtactggttt cgttggggct ggtgctcctt cggctccgtg gggctgccac cgagaagtcg
     4021 ccagtcgtct aaccgacgac tggtagcagg cccgctccga tgcggcgcac taaccatcga
     4081 aacctcgtga atgtcggtat cctgtctggc aggataccgc tcatttccct tgttcagttc
     4141 atcgccgtcg ccgagcatct gaattttcgg catgcggcca aggcacttgg tatcagccag
     4201 tcgagcgtca gcgcgcgtgt gaaagcgctg gaggataacc ttggtgtcct gctatttgag
     4261 cgccatgcgc ggggcgttcg gctaacagac gcaggcaggc acttcatgga gcgtgtcacg
     4321 gcgggtgtcg atcaactcga tcacgcagtg aagaccgcgg agtgacgggc actggctggc
     4381 aatgtctagc aacggcaggc atttcggctg agggtaaaag aactttccgc taagcgatag
     4441 actgtatgta aacacagtat tgcaaggacg cggaacatgc ctcatgtggc ggccaggacg
     4501 gccagccggg atcgggatac tggtcgttac cagagccacc gacccgagca aacccttctc
     4561 tatcagatcg ttgacgagta ttacccggca ttcgctgcgc ttatggcaga gcagggaaag
     4621 gaattgccgg gctatgtgca acgggaattt gaagaatttc tccaaagccc gcgacgcagc
     4681 gccggcaggc agagcaagta gagggcagcg cctgcaatcc atgcccaccc gttccacgtt
     4741 gttatagaag ccgcatagat cgccgtgaag aggaggggtc cgacgatcga ggtcaggctg
     4801 gtgagcgccg ccagtgagcc ttgcagctgc ccctgacgtt cctcatccac ctgcctggac
     4861 aacattgctt gcagcgccgg cattccgatg ccacccgaag caagcaggac catgatcggg
     4921 aacgccatcc atccccgtgt cgcgaaggca agcaggatgt agcctgtgcc gtcggcaatc
     4981 attccgagca tgagtgcccg cctttcgccg agccgggcgg ctacagggcc ggtgatcatt
     5041 gcctgggcga gtgaatgcag aatgccaaat gcggcaagcg aaatgccgat cgtggtcgcg
     5101 tcccagtgaa agcgatcctc gccgaaaatg acccaaagcg cggccggcac ctgtccgaca
     5161 agttgcatga tgaagaagac cgccatcagg gcggcgacga cggtcatgcc ccgggcccac
     5221 cggaacgaag cgagcgggtt gagagcctcc cggcgtaacg gccggcgttc gcctttgtgc
     5281 gactccggca aaaggaaaca gcccgtcagg aaattgaggc cgttcaaggc tgccgcggcg
     5341 aagaacggag cgtgggggga gaaaccgccc atcagcccac cgagcacagg tcccgcgacc
     5401 atcccgaacc cgaaacaggc gctcatgaag ccgaagtgcc gcgcgcgctc atcgccatca
     5461 gtgatatcgg caatataagc gccggctacc gccccagtcg ccccggtgat gccggccacg
     5521 atccgcccga tatagagaac ccaaaggaaa ggcgtcgtcg ccatgatggc gtagtcgaca
     5581 gcagcgccgg ccagcgagac gagcaagacc ggccgccgcc cgaaacgatc cgacagcgcg
     5641 cccagcacag gtgcgcaggc aaattgcatc aacgcataca gcgccagcag aatgccatag
     5701 tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag caccggcata
     5761 atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat caggggtctg
     5821 ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac gcgcggattc
     5881 tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg tcaagcatga
     5941 caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg
     6001 tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag ccggcgcttt
     6061 actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg
     6121 agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt ctgatcggga
     6181 atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg
     6241 gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg
     6301 aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc tacttcactg
     6361 ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc ggcggcaccg
     6421 ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc gacgaagccg
     6481 atccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg gcgaaaagga
     6541 ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca ggaccgctgc
     6601 cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc cccctttcca
     6661 ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc ctagcgtcca
     6721 agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcctgct gcggcgtccg
     6781 ctcgtgggcc gtggcgcggg tccgcgcgcc ggcctcgtgc gcctggcgct cgcgggcgag
     6841 gtccagggcg gccgtcttca cgttctgcct tgcgcagatg agatagatct agcgtggact
     6901 caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg ggcaccgcag
     6961 ggaaattctc gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt
     7021 ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca ggggtcgttt gcgggaaggg
     7081 gcggaatcct acgctaaggc tttggccagc gatattctcc ggtgagattg atgtgttccc
     7141 aggggatagg agaagtcgct tgatatctag tatgacgtct gtcgcacctg cttgatcgcg
     7201 gccgcgatag ctagatcgcg ttgctcctct tctccatccg cgttccaagc tgcggaaagg
     7261 cacccataag cgtacgcctg gtcgagcagg cgacgcggat cgacgtccag cgcacgagag
     7321 aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac aaaggtcgtc tctgtcagcc
     7381 ggatcgtaga acatattggc ggcgccaaag cccacttcac cgaccagacc gacgggatct
     7441 atcaccagcc agccgcgact ggagaacatg atgttttcat gatgcagatc gccatgtagc
     7501 ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta taatcgccgc gtggacgtag
     7561 tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct gaaacaaagc tgcaaagcga
     7621 tcccggatcg ggagaagggc agaaggcagg ggttcctcag atgcggcata cagcttcgcc
     7681 attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag
     7741 agcattcgct ccccggcata ttcgagcaac atcagattgt tctcacgacc gagcaaccgg
     7801 actgctcccc tcccattgcg ccataccaga tagtcggccc cgcgcagttc atcagcaatg
     7861 tcttctatag gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc
     7921 caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca
     7981 ggaaaaacag gcggcatgaa catcaacccc aagtcagagg gtccaatcgc agatagaagg
     8041 caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc
     8101 gctctgcttc atctggcgct gcccagttct cttcggcgtt agcaatcatg agtgccaaat
     8161 cggcatagcg atctgctgtt ccgagccgcc caaggtcgat cagacccgtg cattgaagag
     8221 ttttagggtc caccatgaag ttcggcatgc agggatcacc atggcaaaca accatatcgg
     8281 tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa agatcgagct
     8341 gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg cgggacacca
     8401 catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaacggacat tgatcaaccg
     8461 ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg agcaaatccg
     8521 ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag catgcaccct
     8581 cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct ttgagccaaa
     8641 tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg attttcgcga
     8701 aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca
     8761 accagtcaga atgcgattca ccaaaaaaaa tattagttcg attcaatgga ggttccttca
     8821 gttttctgat gaagcgcgaa tatagagaaa tatcccgaat gtgcagttaa cgaattcttg
     8881 cggtttcttt tagcgccgcc aataccgcca gcccgtcgcg caaggggcgc ggctcgtgtg
     8941 tgcggatgaa gtcagctcca cctgcggcgg cggcaagctc tgcagcgagt gtcgcggccc
     9001 cgacatcccc cggaccacgg cctgtgagcg cgcgcagaaa ggatttgcgc gaaacagaca
     9061 gaagcaccgg caaatcgaag cgcagccgca attcatcgaa ccgcgccagc accgagagcg
     9121 aggtttcggg agcagccccc agaaaaaacc ccatgccggg atcaaggaca aggcggttgc
     9181 gtttgatacc ggcacccgtc agcgccgcga tgcgcgcgtc aaagaacgcc gcaatgtgat
     9241 ccatgatgtc gccagcgggt gcctcgcgcc gatctgcctg cccgtcttgc accgaatgca
     9301 taacgacgag tttggcagat gatttcgcca attgcggata gaacgcagcg tctggaaaac
     9361 cgcgaatatc attgagatag gccacaccac gcgacaaggc ataggcttgc gtcgcgggtt
     9421 gataactgtc gagcgagacg ggaatgccat ctgccttgag cgcgtccagc accggcgcga
     9481 tacgctcgat ttctgtgtcg gacgaaacag gcgcggcgtc ggggttgctg gatgccggac
     9541 cgaggtcgat cacatctgcc ccctcggcca tcaacttacg cgcctgcgca atggctgcgt
     9601 ctggcgccag ataccggcct ccatcggaga aactgtccga ggttatgttg acgatgccga
     9661 aaatgatgag cgatttattc atgggggctt ctataataat aataatcgag catgagtctc
     9721 atacggatgc tcgggtcgaa agggaatccc caggcgagta acctgtttgc ggtgatccat
     9781 tagctgcagg agcagaatag catacatctg gaagcaaagc caggaaagcg gcctatggag
     9841 ctgtgcggca gcgctcagta ggcaattttt caaaatattg ttaagccttt tctgagcatg
     9901 gtatttttca tggtattacc aattagcagg aaaataagcc attgaatata aaagataaaa
     9961 atgtcttgtt tacaatagag tggggggggt cagcctgccg ccttgggccg ggtgatgtcg
    10021 tacttgcccg ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc
    10081 tcgcgcaccc gctggcggcg cttgcgcatg gtcgaaccac tggcctctga cggccagaca
    10141 tagccgcaca aggtatctat ggaagccttg ccggttttgc cggggtcgat ccagccacac
    10201 agccgctggt gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg
    10261 atgcgcacat gctggccgcc acccatgacg gcctgcgcga tcaaggggtt cagggccacg
    10321 tacaggcgcc cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa cccctgccgc
    10381 ttgcggccat tctgggcgat gatggatacc ttccaaaggc gctcgatgca gtcctgtatg
    10441 tgcttgagcg ccccaccact atcgacctct gccccgattt cctttgccag cgcccgatag
    10501 ctacctttga ccaccatggc atcagcggtg acggcctccc acttgggttc caggaacagc
    10561 cggagctgcc gtccgccttc ggtcttgggt tccgggccaa gcactaggcc attaggccca
    10621 gccatggcca ccagcccttg caggatgcgc agatcatcag cgcccagcgg ctccgggccg
    10681 ctgaactcga tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg
    10741 cgctcgcccc gcttgagggc acggaacagg ccgggggcca gacagtgcgc cgggtcgtgc
    10801 cggacgtggc tgaggctgtg cttgttctta ggcttcacca cggggcaccc ccttgctctt
    10861 gcgctgcctc tccagcacgg cgggcttgag caccccgccg tcatgccgcc tgaaccaccg
    10921 atcagcgaac ggtgcgccat agttggcctt gctcacaccg aagcggacga agaaccggcg
    10981 ctggtcgtcg tccacacccc attcctcggc ctcggcgctg gtcatgctcg acaggtagga
    11041 ctgccagcgg atgttatcga ccagtaccga gctgccccgg ctggcctgct gctggtcgcc
    11101 tgcgcccatc atggccgcgc ccttgctggc atggtgcagg aacacgatag agcacccggt
    11161 atcggcggcg atggcctcca tgcgaccgat gacctgggcc atggggccgc tggcgttttc
    11221 ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc
    11281 gcgcttgagg ccgtcgaacc actccggggc catgatgttg ggcaggctgc cgatcagcgg
    11341 ctggatcagc aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag
    11401 ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg
    11461 gccggtgggc agttcgccca cctccagcag atccggcccg cctgcaatct gtgcggccag
    11521 ttgcagggcc agcatggatt taccggcact gttgcaaagt tagcgatgag gcagcctttt
    11581 gtcttattca aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg
    11641 gatcaccata ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac
    11701 ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ccgattatcc
    11761 gtttcagttt gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt
    11821 caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg
    11881 gcgctttatc cgtgttgatg aatcgcggga tctgccactt cttcacgttg ttgaggattt
    11941 tacccagaaa ccggtatgca gctttgctgt tacgacggga ggagagataa aaatcgacag
    12001 tgcggccccg gctgtcgacg gcccggtaca gatacgccca gcggccattg accttcacgt
    12061 aggtttcatc catgtgccac gggcaaagat cggaagggtt acgccagtac cagcgcagcc
    12121 gtttttccat ttcaggcgca taacgctgaa cccagcggta aatcgtggag tgatcgacat
    12181 tcactccgcg ttcagccagc atctcctgca gctcacggta actgatgccg tatttgcagt
    12241 accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca
    12301 tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag
    12361 tgccgctcca tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg
    12421 ccatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
    12481 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccaa acaggaaacg
    12541 cagctgaaac gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc
    12601 aaccagcaag gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg
    12661 acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag
    12721 aaagccatgc tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc
    12781 cggcaattcc tttccctgct ctgccataag gggtctgacg ctcagtggaa cgaaaactgg
    12841 cactgttgca aagttagcga tgaggcagcc ttttgtctta ttcaaaggcc ttacatttca
    12901 aaaactctgc ttaccaggcg catttcgccc aggggatcac cataataaaa tgctgaggcc
    12961 tggcctttgc gtagtgcacg catcacctca atacctttga tggtggcgta agccgtcttc
    13021 atggatttaa atcccagcgt ggcgttgatt atccgtttca gtttgccatg atcgcattca
    13081 atcacgttgt tccggtactt aatctgtcgg tgttcaacgt cagacgggca ccggccttcg
    13141 cgtttgagca gagcaagcgc gcgaccatag gcgggcgctt tatccgtgtt gatgaatcgt
    13201 gggatctgcc acttcttcac gttgttgagg attttaccca gaaaccggta tgcagctttg
    13261 ctgttacgac gggaggagag ataaaaatcg acagtgcggc cccggctgtc gacggcccgg
    13321 tacagatacg cccagcggcc attgaccttc acgtaggttt catccatgtg ccacgggcaa
    13381 agatcggaag ggttacgcca gtaccagcgc agccgttttt ccatttcagg cgcataacgc
    13441 tgaacccagc ggtaaatcgt ggagtgatcg acattcactc cgcgttcagc cagcatctcc
    13501 tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg
    13561 tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga
    13621 tgataagttt atcaccaccg actatttgca acagtgccga gattttattg cttcctgcgg
    13681 gtctgtcggg tgccccaaag aacgataaac cttaatccgg tttgcctgcc agtcagcacc
    13741 ctgtagtaat cttgcacgag gatctcccca ccggttactg ccggtaacgt agacatccct
    13801 ccgcctcaga ctatcctgca gtttactgag aaagcagagc gtgtatcccc tgcgggtgat
    13861 atgtttttcc ttgttaatca ccagccgttt ccatgaccga ctgataattt ccgttggtgc
    13921 gtcgtcaaaa aactgccgcc gtgagctgaa ctcccggctg aggtagtcac aggcattcag
    13981 agtggtaacc ccggcaggtg cggatgaaaa tttaacggta ttcagcagat ggggcaggaa
    14041 acgacgaacg cgcccgtact gctccaccat ttcttcatga aaattatcgt ctgagggccg
    14101 ggcaatttca cggacaagcg tgatgatttc agccagcttt tgccttggga tgtagctgaa
    14161 cacctcagca cgaatcgatt cgtccggtgt ttcttctttc agcaggtacg aacatgcgct
    14221 ggcgagcgcc aatgcagatt tatccagatc cttcagcgag cggagccgtt ttttctgccc
    14281 aatctttctg gcgtcacgga tgataacggc cagcatggcg tccagaacgt ccaatgcatc
    14341 atccagcgcc agcgtttccc atgcaaggac aaaggcaacc agaaccgcca tccttttctg
    14401 cggtgacatc ctggcaatat tgaacaccga agtcatacca gcataacgtg cgagattttt
    14461 caggcgcaca gccgggagtg tactcaggtt ttcagcatgc aggccaaaat cgttcagagt
    14521 tttccagcgt tcaattgctt cattaaacgc cggaccactg atggtcacag ggcccttttt
    14581 cagtgattcc agtaaagaca ggcggctgca atcagttggc cccagcagca tctccagctg
    14641 tgaacgctgt tcggctgacg gtatcagtgc cagtttgttc cacaggcgca acgtcgcctt
    14701 ttcccttacc tctgaaatca accgggtcag cgtagtggct ccggggagaa taatacgatg
    14761 ttgcataagc caccctgtcg ccagatcgaa aagcaggcca ggacgttcgt tgcttatcca
    14821 gctccgggta tataaaagac gggtaaggcg aaatgtccag ggccaggcaa attcacgata
    14881 ctgatagtgc tgacgtatca gcgctgcatg ctcacggcgg gtattttccc tctgaccgta
    14941 ttctgcaaga acggtgatat cacgaatccc gagctgtctg gcggtaaaat gccggacgcc
    15001 ggaaggaata tgacctggga cctacgtgcg cccgcaccga caccctcaca ccttcgagct
    15061 actgttgcca ttaaggggtc gtttcgtggt gctgaatttt gacgatcggg gtaccgtcac
    15121 ccatcgggcg atattggggg aaacctgtac ggtgctggag atggccgcag gaacctggca
    15181 tgccgtgctg tcgctggata ccggtggcat aatttttgaa gtaaaacacg gtggctatca
    15241 acccgtggct gccgatgact atgcgcactg ggctccagcg gaaggagaac caggaaccac
    15301 ggagcttatg gcctggtatg cgcaagcgca ggtgggcgac agcacttttg ccgtctaagg
    15361 cgataaacaa aaacggaatg agtttcccca ttccgtttcc gctattacaa accgtcggtg
    15421 acgattttag ccgccgacgc taatacatcg cgacggcttt ctgccttagg ttgaggctgg
    15481 gtgaagtaag tgaccagaat cagcggcgca cgatcttttg gccagatcac cgcgatatcg
    15541 ttggtggtgc catagccacc gctgccggtt ttatccccca caacccagga agcaggcagt
    15601 ccagcctgaa tgctcgctgc accggtggta ttgcctttca tccatgtcac cagctgcgcc
    15661 cgttggctgt cgcccaatgc tttacccagc gtcagattcc gcagagtttg cgccattgcc
    15721 cgaggtgaag tggtatcacg cggatcgccc ggaatggcgg tgtttaacgt cggctcggta
    15781 cggtcgagac ggaacgtttc gtctcccagc tgtcgggcga acgcggtgac gctagccggg
    15841 ccgccaacgt gagcaatcag cttattcatc gccacgttat cgctgtactg tagcgcggcc
    15901 gcgctaagct cagccagtga catcgtccca ttgacgtgct tttccgcaat cggattatag
    15961 ttaacaaggt cagatttttt gatctcaact cgctgattta acagattcgg ttcgctttca
    16021 cttttcttca gcaccgcggc cacggccatc actttactgg tgctgcacat cgcaaagcgc
    16081 tcatcagcac gataaagtat ttgcgaatta tctgctgtgt taatcaatgc cacacccagt
    16141 ctgcctcccg actgccgctc taattcggca agtttttgct gtacgtccgc cgtttgcgca
    16201 tacagcggca cacttcctaa caacagcgtg acggttgccg tcgccatcag cgtgaactgg
    16261 cgcagtgatt ttttaaccat gggattcctt attctggaag atacgaaata acaacaacat
    16321 gaatagtccc taaattccac gtgtgttttt tattagcttc aaaaatcact atttcacgaa
    16381 gaatttagac tgcttctcac acattgtaac attatttaca accacctttc aatcattttt
    16441 gataaatcat tgatttcatc tttgctgcaa tgatacttaa taaactctgc aagttatcca
    16501 cagagcaaca ctcaatttta ttgatgatat tcttattata ccagacattt ttcatacact
    16561 cccttgtacg gatagttttc cgacaacttc atgattacat atcttgcggt tttgattatt
    16621 tttgctgcaa gaaatacata cttcaaacga aaggtcttta tttgctgtct gtattctgaa
    16681 gagtccaagg aatcaaactt gaacaacaaa aataggttat atgaaagcat catcatttga
    16741 aacacggctt cattcgccca aaatgacttt agcaagagat gacccaccgc catgtcgtat
    16801 ttggcttctt tgatatagtt ttcagggcac tgttgcaaag ttagcgatga ggcagccttt
    16861 tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat ttcgcccagg
    16921 ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat cacctcaata
    16981 cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc gttgattatc
    17041 cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt
    17101 tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg accataggcg
    17161 ggcgctttat ccgtgttgat gaatcgtggg atctgccact tcttcacgtt gttgaggatt
    17221 ttacccagaa accggtatgc agctttgctg ttacgacggg aggagagata aaaatcgaca
    17281 gtgcggcccc ggctgtcgac ggcccggtac agatacgccc agcggccatt gaccttcacg
    17341 taggtttcat ccatgtgcca cgggcaaaga tcggaagggt tacgccagta ccagcgcagc
    17401 cgtttttcca tttcaggcgc ataacgctga acccagcggt aaatcgtgga gtgatcgaca
    17461 ttcactccgc gttcagccag catctcctgc agctcacggt aactgatgcc gtatttgcag
    17521 taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt gaatgggttc
    17581 atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact atttgcaaca
    17641 gtgccgattt acgtgcatag ccgattttca ttcttttctc gctaattagt tatggggtta
    17701 ttgttatgtt gatacagtaa cgagttttgt tacatgaggg gagtcatttt tcgggagaag
    17761 tcaggacttt tcaagactgt cacaaaaacc atcgtttttg atacattaat ttaaccaata
    17821 ggttgcagat caaatcgcct gtaacagcct ttctggctgt ttgtcgtttt cagaagacgg
    17881 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tccatcaggc
    17941 aacgacgggc tgctgccggc catcagcgga cgcagggagg actttccgca accggccgtt
    18001 cgatgcggca ccgatggcct tcgcgcaggg gtagtgaatc cgccaggatt gacttgcgct
    18061 gccctacctc tcactagtga ggggcggcag cgcatcaagc ggtgagcgca ctccggcacc
    18121 gccaactttc agcacatgcg tgtaaatcat cgtcgtagag acgtcggaat ggccgagcag
    18181 atcctgcacg gttcgaatgt cgtaaccgct gcggagcaag gccgtcgcaa acgagtggcg
    18241 gagggtgtgc ggtgtggcgg gcttcgtgat gcctgcttgt tctacggcac gtttgaaggc
    18301 gcgctgaaag gtctggtcat acatgtgatg gcgacgcacg acaccgctcc gtggatcggt
    18361 cgaatgcgtg tgctgcgcaa aaacccagaa ccacggccag gaatgcccgg cgcgcggata
    18421 cttccgctca agggcgtcgg gaagcgcaac gccgctgcgg ccctcggcct ggtccttcag
    18481 ccaccatgcc cgtgcacgcg acagctgctc gcgcaggctg ggtgccaagc tctcgggtaa
    18541 catcaaggcc cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa
    18601 atccagatcc ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg ttccatacag
    18661 aagctgggcg aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc
    18721 cggggtcagc accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca
    18781 gggcagatcc gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg
    18841 atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc
    18901 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
    18961 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
    19021 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
    19081 ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
    19141 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
    19201 ccctaaaaca aagttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
    19261 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagttagac
    19321 atcatgagca acgcaaaaac aaagttaggc atcacaaagt acagcatcgt gaccaacagc
    19381 aacgattccg tcacactgcg cctcatgact gagcatgacc ttgcgatgct ctatgagtgg
    19441 ctaaatcgat ctcatatcgt cgagtggtgg ggcggagaag aagcacgccc gacacttgct
    19501 gacgtacagg aacagtactt gccaagcgtt ttagcgcaag agtccgtcac tccatacatt
    19561 gcaatgctga atggagagcc gattgggtat gcccagtcgt acgttgctct tggaagcggg
    19621 gacggacggt gggaagaaga aaccgatcca ggagtacgcg gaatagacca gttactggcg
    19681 aatgcatcac aactgggcaa aggcttggga accaagctgg ttcgagctct ggttgagttg
    19741 ctgttcaatg atcccgaggt caccaagatc caaacggacc cgtcgccgag caacttgcga
    19801 gcgatccgat gctacgagaa agcggggttt gagaggcaag gtaccgtaac caccccatat
    19861 ggtccagccg tgtacatggt tcaaacacgc caggcattcg agcgaacacg cagtgatgcc
    19921 taacccttcc atcgaggggg acgtccaagg gctggcgccc ttggccgccc ctcatgtcaa
    19981 acgttatgca gccaaatccc aacaattaag ggtcttaaaa tggtaaaaga ttggattccc
    20041 atctctcatg ataattacaa gcaggtgcaa ggaccgttct atcatggaac caaagccaat
    20101 ttggcgattg gtgacttgct aaccacaggg ttcatctctc atttcgagga cggtcgtatt
    20161 cttaagcaca tctacttttc agccttgatg gagccagcag tttggggagc tgaacttgct
    20221 atgtcactgt ctggcctcga gggtcgcggc tacatataca tagttgagcc aacaggaccg
    20281 ttcgaagacg atccgaatct tacgaacaaa agatttcccg gtaatccaac acagtcctat
    20341 agaacctgcg aacccttgag aattgttggc gttgttgaag actgggaggg gcatcctgtt
    20401 gaattaataa ggggaatgtt ggattcgttg gaggacttaa agcgccgtgg tttacacgtc
    20461 attgaagact agtcctttgc ataacaaagc catcaaaccg gacgccagag attccgcgcc
    20521 tgttgcgcat ggcttcgcca ttttatgcgc aataggcgcg ccaccctgtc gccgtttatg
    20581 gcggcgttaa cccaaaggag tatcgtgaaa atatcactaa tggctgcaaa agcaagaaat
    20641 ggggttattg gctgcggctc ggatatcccg tggaacgcta aaggtgagca gctgcttttt
    20701 aaagcaataa cttacaatca atggctctta gtcggccgta aaacatttga ggcaatgggg
    20761 gctctcccaa atagaaagta tgcagttgtc agccgctcag gatcggtagc tactaacgat
    20821 gatgtggttg tgtttccatc tatagaagca gcaatgaggg agctaaagac tcttacgaac
    20881 catgttgttg tttctggtgg tggagagatc tacaagagtc tgatcgccca tgccgacacg
    20941 ctacatatct cgacaataga ttccgagcca gagggcaatg ttttctttcc ggaaatcccc
    21001 aaagagttca atgtggtgtt cgagcaggaa tttcattcaa atataaatta tcgctatcaa
    21061 atctggcaaa ggggttaacc atccaagcca tcggacacat tttgcttcgc tgcgctcaaa
    21121 acgcaaaatg tgccgctgct tagcggcgtt agatgcacta agcacataat tgctcacagc
    21181 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctac
//
