LOCUS       NZ_CP110982             2445 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain XYEH3934 plasmid pYUXYEH3934-4, complete
            sequence.
ACCESSION   NZ_CP110982
VERSION     NZ_CP110982.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31720681
            Assembly: GCF_026248885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of E. coli XYEH3934
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2022) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:38:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,800
            CDSs (total)                      :: 4,680
            Genes (coding)                    :: 4,479
            CDSs (with protein)               :: 4,479
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 67 of 201
            Pseudo Genes (incomplete)         :: 126 of 201
            Pseudo Genes (internal stop)      :: 45 of 201
            Pseudo Genes (multiple problems)  :: 34 of 201
            Pseudo Genes (short protein)      :: 3 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110982.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="XYEH3934"
                     /isolation_source="secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pYUXYEH3934-4"
                     /geo_loc_name="China: Xuyi"
                     /lat_lon="33.01 N 118.54 E"
                     /collection_date="2021-09-19"
                     /collected_by="Guo-Zhuang Sun"
     gene            join(2183..2445,1..220)
                     /locus_tag="ORI33_RS23875"
                     /old_locus_tag="ORI33_23870"
     CDS             join(2183..2445,1..220)
                     /locus_tag="ORI33_RS23875"
                     /old_locus_tag="ORI33_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            207..437
                     /locus_tag="ORI33_RS23880"
                     /old_locus_tag="ORI33_23875"
     CDS             207..437
                     /locus_tag="ORI33_RS23880"
                     /old_locus_tag="ORI33_23875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_266059242.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKTAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(507..1475)
                     /gene="mobV"
                     /locus_tag="ORI33_RS23885"
                     /old_locus_tag="ORI33_23880"
     CDS             complement(507..1475)
                     /gene="mobV"
                     /locus_tag="ORI33_RS23885"
                     /old_locus_tag="ORI33_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     Typing          complement(511..1484)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tcatattgaa tatcagacct ttaaaaacat aattgcggct cttgtagata aacaaatatt
       61 cttccgggca gggcgtgggg aacttgaagc taaccccttt ctttttgcac ggggagactg
      121 gccatcaatt tacgaaagaa gaaagaaaat tgagttaaag atcagttatg acaccaatgg
      181 aagaacggtg cagacgaagg taatggatga tgaatgctga agataaactt tttaaaaatg
      241 tagacaggct aatgctgcat gattcactaa aaaagaatga ggttctgact gtttgggagc
      301 gtactttttt aagcagtgtt tacagcatat atcgtagaac tagcggtaat aaatactcaa
      361 ctaagggttt gtcaccaaag cagaaaaccg cagctttttc tattttaaaa aaacataatt
      421 accatctgaa tgattaattt tttcgtggtg aatgccgcaa atgacgtcac tttgcggcgt
      481 tagccctatc ctgcatcgca tcaaaactaa cgctccatac cacccatgcc acgcgatcgc
      541 gtctgtcgct gtttttcacg ttcaatacgc tgctgttttt ccttttcctg gttttgctgc
      601 ttaaaccgct gagccatcat caggatttcg gtcatctgct ctctggaaag ccccttaaaa
      661 ggctctgtga cgctctgtag gcgtttctgc tgcgcatcca tagtcttctg tagctcacgc
      721 gctctttttg cgttctgggc gctctgagag gccatagcga tggttcctgc aaagccatcg
      781 ttcactgcgt ggcttagacg tttggctatg gctgcctgat cttccacgtc tttggtgaaa
      841 atgcccttcc gcaatacacg cggctccagc gcctccggcg agatcgatac ctggctacgg
      901 gtgccacggt ttatcgattc gtagtagtgc tgaactgtct gatgcgtggc gcggcttccc
      961 tcgatgccgc gctcaagacc cagttttttt actgattcag cgtaggtgct ctgatcttca
     1021 cgcatctttg accgcccgcc aataaactct ttcgcggaca gccgtccatc ctgcgtcaat
     1081 ggcaccacga acgcagacag gtgcggggtg gcctcgtccc ggtgaaccac cgccgccaca
     1141 acacgatcct tcccgtattt cttctccagc cattgctcag agcgggcgaa aaactccgcc
     1201 tgctgccgtg gtgtggcctc tttccaccac tcagggcttg ctgtcataac gtattcaacg
     1261 gccagtaccg catctttacg gcgtttttcc ggcagcagct cccgcacacg ccccattgcc
     1321 tgatccgttg atttggacac agagcagtag ttttctggtg tccgttcagc gttagcgttc
     1381 ggcgtctcac gctctcggta gcagtgctgc aacgcgctgg ccacgcttcc cattcccgtc
     1441 agctttttgc agcgcattat cgcgtagttc gccatgttcc cccctgattc cggcattcat
     1501 tcagccaggc gggggagcgc attgcgatac ctccggcggg ccactatcaa gatgatagtg
     1561 taaaactcac tagactttgc tacgcaaaat cgttcgggca gcaagctgcc agaaggtcaa
     1621 aatcaacggc ggtctggcag gtggaaggcg tcgcttcgcg ccacccccca ccactgccaa
     1681 gcctgtttta tccggagtgt cgtcaagggt aaatggcact cgctacgctc gcccttgacg
     1741 aaatcaggca atgaaatcgt ctttgaaaga gcaggaaccg ctcgccgcag ctttggctga
     1801 ggcgtaccgc aggagcaacg cgacgaggaa cgaacagcca aagcgaggaa gcggaatttc
     1861 cactccccgt acatagaaat gcgtacactc cttgctaaaa aaaggcagtt tttcagtgtc
     1921 taaaaatgcc gtttttcagc ccttattttc ccccttaata tatagatata gatatgggaa
     1981 cccgcttgta ccaagggcta gagcgaagaa tcgtactcac ctcaagtacc aatcgtactc
     2041 acctcaagta ctaatcgtac tcacctcaag gactaatcgt actcacctca agtactaatc
     2101 gtacttgata caaaggactt gactgtatgt ctctttagta cttaaatgcg agtactaaat
     2161 catggcgaaa cgtggggctg gagtgaatac gaaaaagatt tctaggcttg aacggcatac
     2221 atacatggat gaaaacggtg aggtaaaaca agactctaag atcgagacct tttccatccc
     2281 agccgaacct ccctacgtca agctatacat tgcagacatt gcgatgattc atggtttatc
     2341 aaaatctcaa aatgatgtgc ttttacaatt ggcaatgctg attggatggg atgggtttgt
     2401 gtctgtatca aaaactagat ttgaaaaaac catacagccc agggt
//
