LOCUS       NZ_CP110912            11156 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter freundii strain CFA1707 plasmid pCFA1707-7, complete
            sequence.
ACCESSION   NZ_CP110912
VERSION     NZ_CP110912.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693747
            Assembly: GCF_026240875.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026240875.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:41:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,699
            CDSs (total)                      :: 5,577
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 41 of 134
            Pseudo Genes (incomplete)         :: 89 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 18 of 134
            Pseudo Genes (short protein)      :: 1 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110912.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CFA1707"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCFA1707-7"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-07-28"
                     /collected_by="zhangxiaofan"
     gene            complement(join(10978..11156,1..601))
                     /locus_tag="OQZ20_RS02560"
                     /old_locus_tag="OQZ20_02555"
     CDS             complement(join(10978..11156,1..601))
                     /locus_tag="OQZ20_RS02560"
                     /old_locus_tag="OQZ20_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_109018074.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMMSWLSDKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTEQE
                     LLSIYKETLNQLNAVAIFDCPFISSIDHRLKESKFFIDNQLLDEIDQDDFEAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKSGEIYFLDLGRAGLADEFVDISFVE
                     RCLREDVSEETAKIFLKHLKNDMPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(847..>1146)
                     /locus_tag="OQZ20_RS02565"
                     /old_locus_tag="OQZ20_02560"
                     /pseudo=""
     CDS             complement(847..>1146)
                     /locus_tag="OQZ20_RS02565"
                     /old_locus_tag="OQZ20_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /name="enoyl-CoA hydratase/isomerase family protein"
     gene            1204..1901
                     /locus_tag="OQZ20_RS02570"
                     /old_locus_tag="OQZ20_02565"
     CDS             join(1204..1453,1453..1901)
                     /locus_tag="OQZ20_RS02570"
                     /old_locus_tag="OQZ20_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(2640..3170)
                     /locus_tag="OQZ20_RS02575"
                     /old_locus_tag="OQZ20_02570"
     CDS             complement(2640..3170)
                     /locus_tag="OQZ20_RS02575"
                     /old_locus_tag="OQZ20_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(3173..4108)
                     /locus_tag="OQZ20_RS02580"
                     /old_locus_tag="OQZ20_02575"
     CDS             complement(3173..4108)
                     /locus_tag="OQZ20_RS02580"
                     /old_locus_tag="OQZ20_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            4275..4661
                     /locus_tag="OQZ20_RS02585"
                     /old_locus_tag="OQZ20_02580"
     CDS             4275..4661
                     /locus_tag="OQZ20_RS02585"
                     /old_locus_tag="OQZ20_02580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            complement(5473..6252)
                     /locus_tag="OQZ20_RS02590"
                     /old_locus_tag="OQZ20_02585"
     CDS             complement(5473..6252)
                     /locus_tag="OQZ20_RS02590"
                     /old_locus_tag="OQZ20_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_109018074.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMMSWLSDKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTEQE
                     LLSIYKETLNQLNAVAIFDCPFISSIDHRLKESKFFIDNQLLDEIDQDDFEAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKSGEIYFLDLGRAGLADEFVDISFVE
                     RCLREDVSEETAKIFLKHLKNDMPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(6498..>6797)
                     /locus_tag="OQZ20_RS02595"
                     /old_locus_tag="OQZ20_02590"
                     /pseudo=""
     CDS             complement(6498..>6797)
                     /locus_tag="OQZ20_RS02595"
                     /old_locus_tag="OQZ20_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /name="enoyl-CoA hydratase/isomerase family protein"
     gene            6855..7552
                     /locus_tag="OQZ20_RS02600"
                     /old_locus_tag="OQZ20_02595"
     CDS             join(6855..7104,7104..7552)
                     /locus_tag="OQZ20_RS02600"
                     /old_locus_tag="OQZ20_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(8291..8821)
                     /locus_tag="OQZ20_RS02605"
                     /old_locus_tag="OQZ20_02600"
     CDS             complement(8291..8821)
                     /locus_tag="OQZ20_RS02605"
                     /old_locus_tag="OQZ20_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(8824..9150)
                     /locus_tag="OQZ20_RS28475"
     CDS             complement(8824..9150)
                     /locus_tag="OQZ20_RS28475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_279906115.1"
                     /translation="MKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGRLDDSQRQDI
                     KDSRTFSVVDVPEQAKHRKELVRSLTHDISQQYGREMAARVTKQGNVEIERVKSLSRGF
                     SISR"
                     /name="hypothetical protein"
     gene            complement(9131..9709)
                     /locus_tag="OQZ20_RS02610"
                     /old_locus_tag="OQZ20_02605"
     CDS             complement(9131..9709)
                     /locus_tag="OQZ20_RS02610"
                     /old_locus_tag="OQZ20_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_279906116.1"
                     /translation="MATYHLSVKFGGRVKPLLMPVTLRGKISSASARISSTPNTAICRN
                     GRRDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQWLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPAQSGGARKRDSGH
                     NLTPTEQNSTKAPRERWAYAQRAYETIWL"
                     /name="MobA/MobL family protein"
     ARG             complement(5477..6252)
                     /name="aph(3')-VIb"
                     /gene="aph(3')-VIb"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIb"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.07"
                     /coverage="100.0"
     ARG             complement(10979..11154)
                     /name="APH(3')-VIb"
                     /gene="APH(3')-VIb"
                     /gene_name="APH(3')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="22.39"
     ARG             complement(3..601)
                     /name="APH(3')-VIb"
                     /gene="APH(3')-VIb"
                     /gene_name="APH(3')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.5"
                     /coverage="77.22"
     Typing          complement(3177..4108)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8828..9213)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.272"
                     /coverage="100.0"
                     /evalue="6.47e-63"
ORIGIN
        1 tttcaccaga tttatctata aaaatattac tatccgtgat atcgccatga gaaaaaacca
       61 atctttcttc aacacgagtc tcatttaact cattccaaag acttaggtaa gttttatggt
      121 ctccccataa ttcagcctca aaatcatctt gatctatctc gtcaaggagt tggttatcaa
      181 taaaaaattt tgactctttt aaccgatgat caatgcttga aataaatggg caatcaaaaa
      241 tagcaactgc atttaactga ttgagtgttt ccttatagat agaaagcaat tcttgctctg
      301 ttaaaaaaag cgctgaaatt ggttttgcat tgatcgcttt agtgatcatg aattcaaact
      361 gctcatcctg aaaagtcatg atgagttcag gcacctttaa tttatcagag agccaactca
      421 tcattttcgc ttcacgagag acactgtatg tggtctctgt atataaagtg ctagatcgct
      481 taagaaaaaa agtttcatta tttcgattaa aagaataaac atccgatggc gactgaccaa
      541 ttttatttgg ctctaaaacg ctgtttccaa taaattgttg aataatatta ggcaattcca
      601 tcaagttttc cttttattca gcattaaaaa cctcgcaaat gcgaggccta gtaaatagat
      661 gatcttaatt aggttcactg tagcaaaaat atggcttatt gtctatagag ggtcaatgca
      721 tcaaactttt gtcattacct ctcaattgca taaatagcgc taggcttaag gaaaatgggt
      781 tgctgtattt ctctgtctca aatctcaaga ataagctaga gcagaatatt agacagcagc
      841 aagacctcag gatgcagaca aatcgcggag tagttgctcc aaatcctgtt cgcccacttt
      901 tgtttgagca cctgcctgca agccgccacg aatgagtttt tcagcagccg gacgcgcaaa
      961 cagaccagcc catgccttgt gctctcgaag gaagtcttca cttaaatccg ttggtgggat
     1021 tgctttcttt gcagcggcga tgaccccttc aggtaaagct gcaatatttg tggcgatgcg
     1081 atgaacaaaa gcgtctaact catttgccgg aagcgcacga tttacccaac catatcgttc
     1141 ggctagcagg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     1201 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     1261 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
     1321 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
     1381 gccatgaatg gcgttggatg ccgggcaacc gcccgcatta tgggcgttgg cctcaacacg
     1441 attttccgcc atttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
     1501 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg atacgtcggg gctaaatcgc
     1561 gccagcgctg gctgttttac gcgtatgaca ggctccggaa gacggttgtt gcgcacgtat
     1621 tcggtgaacg cacgatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
     1681 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
     1741 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
     1801 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
     1861 aagtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attaccaccg
     1921 aaatgatgtg aagacttcat cattttagca atcacaggtt gccctaatca cttaacgcta
     1981 ttattgttct attgaagcct taacctaaac ccactcacaa aacgtggatt atctggttag
     2041 tgattcgcag caacaaagcg tcccggttca aaacgaatcg taagacgtag tttcccttga
     2101 tgtagggctt gccgatggaa caggcggctt ttttattctc cggcgtgggg ggggatgctg
     2161 ttgacagaaa tggaaataag taacatccgg aatattacgc ttattgcccg gcgtcttgcc
     2221 tggattaatg ccaaaagaac tcatatcgat ataccatcgt ttgcagaact gcttttctcc
     2281 ggccaaaaag cgctgttctt cgaacaatat aattattttc actcacgacg gtacacaacg
     2341 gcaggcacgt caaagccacc ggatttacca taacatccgt tgcgtgcacc gcctcagagg
     2401 ctctcagggc ggttttaagt cctgagccat aaaaaccccc ggcttctcac cgaaaaccgc
     2461 tcagaatcgc ataccgtgat tttcagcccc atgcataacc atgcacctga ctgactggcg
     2521 agaacttccg gcctgaattc aggccggaag tctcaaaaga cgccccaaac tcggctgaaa
     2581 acggtgaacg ggtgcgcaat ttattgtgcg aacgtgaggc gttggacaag cgcagcgcgt
     2641 cagttctttt tcttttccgg tttctgttcc gttgtcagca gcttttcaag gtctgccact
     2701 ttctgataga cggcagcaga cgcgttgtaa ttgtcctgta gttccgtttt cacaaaccag
     2761 cttgtggcaa atatcagtac gacaaaggca accgcaaccc cagcaaacca cttccagctg
     2821 taaccccgca gcgtgttgta ctccgcgatg atgttactgc ggataatgga aatttccttg
     2881 tccagcgcgt ccgcattgat tttcatgttc ccgacggatt tttccagacg ctgaaaatca
     2941 gcgctgatac gctcgccaat cagccccgca acatccattt tctgcacttc ctgccggact
     3001 tcatccctta cctgctgtgc cacactgtcc gatatttccc ggtagccttt cagggtttcc
     3061 cttgccaccg ccagcgaatc cgccacggct ttctgctgct cttttgcggc ctctttcaga
     3121 tgctccgccg acagcaggag tgtgagaagt ttttcttcgg tgctcgccat agttacctgc
     3181 ttatggaaaa tccgcgtgac agggatttca cgcgctctat ttcaacattt ccctgttttg
     3241 tgacccgtgc agccatctcc cgcccgtact gctgcgagat atcatgcgtc agagatctga
     3301 ccagttcctt ccggtttacc tcgataccgc tggcattcat ccagctacgg tcgctgtggt
     3361 cgatatgttt cgcctgttca ggcacatcca cgactgaaaa cgtccggctg tctttaatat
     3421 cctgcctctg gctgtcgtcc agtcgtccgg ctgcttcaaa accgagatgc tgttccggtt
     3481 tccggtcgat accctgtgtt ttcagtgacc ggctatcaat aaaaccctgc tcaaagccat
     3541 atcgtttcat atgcgcgttg tgcgtacgct cccagcgctc ccgcagggct ttcagtgcct
     3601 gtttttgttc tgtaggggtt aaattatgcc ctgagtcctt gcgcgcgccg ccacgcgccg
     3661 gaaatttggc gttatagcgc ctgaaatact gttcgggact gcgctcaata ccgtcattga
     3721 cgcgctggct catcatgata tgggcgtgcg gctgttcgcc gccgtcaatg ctggctttcg
     3781 gattgtggat cgcaaacgtc catgcatgtt tgtcgcccgt ttcctgcttc acaaaatccc
     3841 ggacaagcgc cagccgctgc gcttcattca gctcgcgggg tagagcgatc tcaatttccc
     3901 tgtacgttga accgttggca cgttcgaacg catccgcagc ctgccagaaa tgagcgggtt
     3961 catctctcgc ccattccggc atattgccgt gttcggtgtg ctcgagatcc tggcgctggc
     4021 tgaacttatc ttcccgcgta atgtaactgg catgagcaag ggctttaccc ttgccgccaa
     4081 atttcacgct gagatgatac gttgccatgt tgtgtcagcc cccggcaggg cgcactgttt
     4141 ctgcccgcag ggagaaacat ataagtgcgc tttactctaa tttaaagaat tatgagtaaa
     4201 gataagtcat gcgacttctg accgcaagtt atatactttc cccgtcggaa atatcgggat
     4261 ggggaagtta aacgatgcgg atcagtaacg tcgaatggct taaaaagcgc atagccttca
     4321 tcagaaagct gggagaaaaa acggcacggc aacggcagat tatcgatctg ctggatgatg
     4381 aagcttcgct cagtgagact gaaagacgac tgctccacgt tctggcaacc gctgaaaaaa
     4441 acgcgttgca ggaacgggat gccgcacaga aaaaggcggt tcaggcgcgg atttcagggc
     4501 agaaaaaccg cagggaacgc aatcacagac tgtttctggc ggctggtctg atgattgacg
     4561 cgggactggt tgatacaaaa acgggtgagt tgaattttga ccacgaaaaa ttactgactg
     4621 cgctgaaacg ggtacgctac gatctggaaa catcggcgtg aacgtgcatt ctgcaaggcc
     4681 ggagaaatgc cgcttcctcg ctcactgact cgcgtcgctc ggtcgttcgg ctgcggcgag
     4741 cggtagggat tttcggatag gagcatgaat tttcttgctc cagatgtaca aaagccccgt
     4801 gacagaatca cggggcttga gtactctaat tcttgcagat acctgccagg atcagacgtc
     4861 acaatcaaat cctagcaggg ctggctagga atgtcactac cagcttaata ccattcaacg
     4921 acattaaacc catatctgca ccttgactct caggtctacg ccccggactc cggcaggata
     4981 aacaggcaag cggtgggaac ctgaccagct cgggaaagaa gctggctcta cccggtctca
     5041 cgttaaccaa taacgtacct gataaagtga gagtgaaggc agcggttgcg gtgaagcgtg
     5101 attgccaagg tctggcgcaa cacccacaaa atcgaagcag catacgaacg gcctgatgct
     5161 ggtcgcttcg ctccccgttt tctggtgtac ccaccacggg ggcattaggc tttctttgcc
     5221 ctaatatggg tttgcgactc tcaaatccgg gttgtggtgt aacacatatc gccttcaccg
     5281 aaatgggtaa tgataccaac gtgttgattt cgtattttca ctgacaccag gatcatcctg
     5341 atgttacaag gattgaatga caacagatta aaatagtcat caacaggttg acagcattac
     5401 cttttttcac ttatttattt aaaaattagc aacttaaaaa tacagctaag tatttttaga
     5461 tattgcttgg agttaattca attcatcaag ttttaaaaaa taattccttt tgtcaggcat
     5521 atcgtttttt aaatgcttta aaaatatttt agcagtttcc tcagatacat cctctcttag
     5581 gcaacgttca acaaaagata tatctacaaa ttcatctgct aatccagcac gaccaagatc
     5641 taaaaagtaa atttcaccag atttatctat aaaaatatta ctatccgtga tatcgccatg
     5701 agaaaaaacc aatctttctt caacacgagt ctcatttaac tcattccaaa gacttaggta
     5761 agttttatgg tctccccata attcagcctc aaaatcatct tgatctatct cgtcaaggag
     5821 ttggttatca ataaaaaatt ttgactcttt taaccgatga tcaatgcttg aaataaatgg
     5881 gcaatcaaaa atagcaactg catttaactg attgagtgtt tccttataga tagaaagcaa
     5941 ttcttgctct gttaaaaaaa gcgctgaaat tggttttgca ttgatcgctt tagtgatcat
     6001 gaattcaaac tgctcatcct gaaaagtcat gatgagttca ggcaccttta atttatcaga
     6061 gagccaactc atcattttcg cttcacgaga gacactgtat gtggtctctg tatataaagt
     6121 gctagatcgc ttaagaaaaa aagtttcatt atttcgatta aaagaataaa catccgatgg
     6181 cgactgacca attttatttg gctctaaaac gctgtttcca ataaattgtt gaataatatt
     6241 aggcaattcc atcaagtttt ccttttattc agcattaaaa acctcgcaaa tgcgaggcct
     6301 agtaaataga tgatcttaat taggttcact gtagcaaaaa tatggcttat tgtctataga
     6361 gggtcaatgc atcaaacttt tgtcattacc tctcaattgc ataaatagcg ctaggcttaa
     6421 ggaaaatggg ttgctgtatt tctctgtctc aaatctcaag aataagctag agcagaatat
     6481 tagacagcag caagacctca ggatgcagac aaatcgcgga gtagttgctc caaatcctgt
     6541 tcgcccactt ttgtttgagc acctgcctgc aagccgccac gaatgagttt ttcagcagcc
     6601 ggacgcgcaa acagaccagc ccatgccttg tgctctcgaa ggaagtcttc acttaaatcc
     6661 gttggtggga ttgctttctt tgcagcggcg atgacccctt caggtaaagc tgcaatattt
     6721 gtggcgatgc gatgaacaaa agcgtctaac tcatttgccg gaagcgcacg atttacccaa
     6781 ccatatcgtt cggctagcag gtgatgctgc caacttactg atttagtgta tgatggtgtt
     6841 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
     6901 gggtggtgcg taacggcaaa agcactgccg gacatcagcg ctatctctgc tctcactgcc
     6961 gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa
     7021 tcattgatat ggccatgaat ggcgttggat gccgggcaac cgcccgcatt atgggcgttg
     7081 gcctcaacac gattttccgc catttaaaaa actcaggccg cagtcggtaa cctcgcgcat
     7141 acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gatacgtcgg
     7201 ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac aggctccgga agacggttgt
     7261 tgcgcacgta ttcggtgaac gcacgatggc gacgctgggg cgtcttatga gcctgctgtc
     7321 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa
     7381 gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa
     7441 tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga
     7501 gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcagt aagttggagt
     7561 cattaccacc gaaatgatgt gaagacttca tcattttagc aatcacaggt tgccctaatc
     7621 acttaacgct attattgttc tattgaagcc ttaacctaaa cccactcaca aaacgtggat
     7681 tatctggtta gtgattcgca gcaacaaagc gtcccggttc aaaacgaatc gtaagacgta
     7741 gtttcccttg atgtagggct tgccgatgga acaggcggct tttttattct ccggcgtggg
     7801 gggggatgct gttgacagaa atggaaataa gtaacatccg gaatattacg cttattgccc
     7861 ggcgtcttgc ctggattaat gccaaaagaa ctcatatcga tataccatcg tttgcagaac
     7921 tgcttttctc cggccaaaaa gcgctgttct tcgaacaata taattatttt cactcacgac
     7981 ggtacacaac ggcaggcacg tcaaagccac cggatttacc ataacatccg ttgcgtgcac
     8041 cgcctcagag gctctcaggg cggttttaag tcctgagcca taaaaacccc cggcttctca
     8101 ccgaaaaccg ctcagaatcg cataccgtga ttttcagccc catgcataac catgcacctg
     8161 actgactggc gagaacttcc ggcctgaatt caggccggaa gtctcaaaag acgccccaaa
     8221 ctcggctgaa aacggtgaac gggtgcgcaa tttattgtgc gaacgtgagg cgttggacaa
     8281 gcgcagcgcg tcagttcttt ttcttttccg gtttctgttc cgttgtcagc agcttttcaa
     8341 ggtctgccac tttctgatag acggcagcag acgcgttgta attgtcctgt agttccgttt
     8401 tcacaaacca gcttgtggca aatatcagta cgacaaaggc aaccgcaacc ccagcaaacc
     8461 acttccagct gtaaccccgc agcgtgttgt actccgcgat gatgttactg cggataatgg
     8521 aaatttcctt gtccagcgcg tccgcattga ttttcatgtt cccgacggat ttttccagac
     8581 gctgaaaatc agcgctgata cgctcgccaa tcagccccgc aacatccatt ttctgcactt
     8641 cctgccggac ttcatccctt acctgctgtg ccacactgtc cgatatttcc cggtagcctt
     8701 tcagggtttc ccttgccacc gccagcgaat ccgccacggc tttctgctgc tcttttgcgg
     8761 cctctttcag atgctccgcc gacagcagga gtgtgagaag tttttcttcg gtgctcgcca
     8821 tagttacctg cttatggaaa atccgcgtga cagggatttc acgcgctcta tttcaacatt
     8881 tccctgtttt gtgacccgtg cagccatctc ccgcccgtac tgctgcgaga tatcatgcgt
     8941 cagagatctg accagttcct tccgatgttt cgcctgttca ggcacatcca cgactgaaaa
     9001 cgtccggctg tctttaatat cctgcctctg gctgtcgtcc agtcgtccgg ctgcttcaaa
     9061 accgagatgc tgttccggtt tccggtcgat accctgtgtt ttcagtgacc ggctatcaat
     9121 aaaaccctgc tcaaagccat atcgtttcat atgcgcgttg tgcgtacgcc cagcgctccc
     9181 gcggggcttt cgtgctgttt tgttctgtag gggttaaatt atgccctgag tcgcgcttgc
     9241 gcgcgccgcc gctttgcgcc ggaaatttgg cgttatagcg cctgaaatac tgttcaggac
     9301 tgcgctcaat accgtcattg acgcgctggc tcatcatgat atgggcgtgc ggctgttcgc
     9361 cgccgtcaat gctggctttc ggattgtgga tcgcaaacgt ccatgcatgt ttgtcgcccg
     9421 tttcctgctt cacaaaatcc cggacaagcg ccagccactg cgcttcattc agctcgcggg
     9481 gtagagcgat ctcaatttcc ctgtacgttg aaccattggc gcgttcgaac gcatccgcag
     9541 cctgccagaa atgagcgggt tcatctcttc gcccattccg gcatattgcc gtgttcggtg
     9601 tgctcgagat cctggcgctg gctgaactta tcttcccgcg taatgtaact ggcatgagca
     9661 agggctttac cctgccgcca aatttcacgc tgagatgata cgttgccatg ttgtgtcagc
     9721 cccggcaggg cgcactgttt ctgcccgcag ggagaaacat ataagcgcgc tttactctaa
     9781 tttaaagaat tatgagtaaa gataagtcat gcgacttctg accgcaagtt atatactttc
     9841 cccgtcggaa atatcgggat ggggaagtta aacgatgcgg atcagtaacg tcgaatggct
     9901 taaaaaagcg catagccttc atcagaaagc tgggagaaaa aacggcacgg caacggcaga
     9961 ttatcgatct gctggatgat gaagcttcgc tcagtgagac tgaaagacga ctgctccacg
    10021 ttctggcaac cgctgaaaaa aacgcgttgc aggaacggga tgccgcacag aaaaaggcgg
    10081 ttcaggcgcg gatttcaggg cagaaaaacc gcagggaacg caatcacaga ctgtttctgg
    10141 cggctggtct gatgattgac gcgggactgg ttgatacaaa aacgggtgag ttgaattttg
    10201 accacgaaaa attactgact gcgctgaaac gggtacgcta cgatctggaa acatcggcgt
    10261 gaacgtgcat tctgcaaggc cggagaaatg ccgcttcctc gctcactgac tcgcgtcgct
    10321 cggtcgttcg gctgcggcga gcggtaggga ttttcggata ggagcatgaa ttttcttgct
    10381 ccagatgtac aaaagccccg tgacagaatc acggggcttg agtactctaa ttcttgcaga
    10441 tacctgccag gatcagacgt cacaatcaaa tcctagcagg gctggctagg aatgtcacta
    10501 ccagcttaat accattcaac gacattaaac ccatatctgc accttgactc tcaggtctac
    10561 gccccggact ccggcaggat aaacaggcaa gcggtgggaa cctgaccagc tcgggaaaga
    10621 agctggctct acccggtctc acgttaacca ataacgtacc tgataaagtg agagtgaagg
    10681 cagcttctgg tgtacccacc acgggggcat taggctttct ttgccctaat atgggtttgc
    10741 gactctcaaa tccgggttgt ggtgtaacac atatcgcctt caccgaaatg ggtaatgata
    10801 ccaacgtgtt gatttcgtat tttcactgac accaggatca tcctgatgtt acaaggattg
    10861 aatgacaaca gattaaaata gtcatcaaca ggttgacagc attacctttt ttcacttatt
    10921 tatttaaaaa ttagcaactt aaaaatacag ctaagtattt ttagatattg cttggagtta
    10981 attcaattca tcaagtttta aaaaataatt ccttttgtca ggcatatcgt tttttaaatg
    11041 ctttaaaaat attttagcag tttcctcaga tacatcctct cttaggcaac gttcaacaaa
    11101 agatatatct acaaattcat ctgctaatcc agcacgacca agatctaaaa agtaaa
//
