LOCUS       NZ_CP110910             8282 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter freundii strain CFA1707 plasmid pCFA1707-8, complete
            sequence.
ACCESSION   NZ_CP110910
VERSION     NZ_CP110910.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693747
            Assembly: GCF_026240875.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026240875.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:41:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,699
            CDSs (total)                      :: 5,577
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 41 of 134
            Pseudo Genes (incomplete)         :: 89 of 134
            Pseudo Genes (internal stop)      :: 23 of 134
            Pseudo Genes (multiple problems)  :: 18 of 134
            Pseudo Genes (short protein)      :: 1 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110910.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CFA1707"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCFA1707-8"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-07-28"
                     /collected_by="zhangxiaofan"
     gene            join(7865..8282,1..314)
                     /locus_tag="OQZ20_RS01145"
                     /old_locus_tag="OQZ20_01140"
     CDS             join(7865..8282,1..314)
                     /locus_tag="OQZ20_RS01145"
                     /old_locus_tag="OQZ20_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            321..851
                     /locus_tag="OQZ20_RS01150"
                     /old_locus_tag="OQZ20_01145"
     CDS             321..851
                     /locus_tag="OQZ20_RS01150"
                     /old_locus_tag="OQZ20_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(879..1058)
                     /locus_tag="OQZ20_RS01155"
                     /old_locus_tag="OQZ20_01150"
     CDS             complement(879..1058)
                     /locus_tag="OQZ20_RS01155"
                     /old_locus_tag="OQZ20_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1128..1505
                     /locus_tag="OQZ20_RS01160"
                     /old_locus_tag="OQZ20_01155"
     CDS             1128..1505
                     /locus_tag="OQZ20_RS01160"
                     /old_locus_tag="OQZ20_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(1567..1866)
                     /locus_tag="OQZ20_RS01165"
                     /old_locus_tag="OQZ20_01160"
     CDS             complement(1567..1866)
                     /locus_tag="OQZ20_RS01165"
                     /old_locus_tag="OQZ20_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1856..2119)
                     /locus_tag="OQZ20_RS01170"
                     /old_locus_tag="OQZ20_01165"
     CDS             complement(1856..2119)
                     /locus_tag="OQZ20_RS01170"
                     /old_locus_tag="OQZ20_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2893..3411
                     /locus_tag="OQZ20_RS01175"
                     /old_locus_tag="OQZ20_01170"
     CDS             2893..3411
                     /locus_tag="OQZ20_RS01175"
                     /old_locus_tag="OQZ20_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704828.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVARSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            complement(3442..3696)
                     /locus_tag="OQZ20_RS01180"
                     /old_locus_tag="OQZ20_01175"
     CDS             complement(3442..3696)
                     /locus_tag="OQZ20_RS01180"
                     /old_locus_tag="OQZ20_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321408.1"
                     /translation="MNFKQLIPAQDWYFRHFTDQSDYPVIVYQLAAWALTEEGSITGLV
                     AIYDPKEGKAKLVTPPPVKGNYLHRDQLTDEELAASRKP"
                     /name="hypothetical protein"
     gene            complement(3709..3960)
                     /locus_tag="OQZ20_RS01185"
                     /old_locus_tag="OQZ20_01180"
     CDS             complement(3709..3960)
                     /locus_tag="OQZ20_RS01185"
                     /old_locus_tag="OQZ20_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321409.1"
                     /translation="MALVQKKTLNELATNASDDFFGKRFSAGDEVPHSGIYKCTKCNRE
                     VTVNAHSRDNTFPPHYPESPCKSPTWKPYVIADTKAEW"
                     /name="hypothetical protein"
     gene            4114..4446
                     /locus_tag="OQZ20_RS01190"
                     /old_locus_tag="OQZ20_01185"
     CDS             4114..4446
                     /locus_tag="OQZ20_RS01190"
                     /old_locus_tag="OQZ20_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321410.1"
                     /translation="MEKQYTNELTAEILAGMDQSPFTPEQLAAMSDEARALIEEQEAFC
                     HAHPVTTIYRLAVAGCLTRRGGTGDEFNPNPEEGHKIRLENGLWVSVLTEGCTVTYPDG
                     TQARIL"
                     /name="hypothetical protein"
     gene            5904..7865
                     /locus_tag="OQZ20_RS01195"
                     /old_locus_tag="OQZ20_01190"
     CDS             5904..7865
                     /locus_tag="OQZ20_RS01195"
                     /old_locus_tag="OQZ20_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     Typing          4722..4910
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.529"
                     /coverage="94.0"
                     /evalue="1.1199999999999999e-60"
     Typing          4..311
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.087"
                     /coverage="99.0"
                     /evalue="6.08e-59"
     Typing          7866..8281
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.086"
                     /coverage="99.0"
                     /evalue="1.71e-69"
ORIGIN
        1 gtcttaaagg tatgaaggtg ccggatgcgc tggcagagct ggacggtaaa acggtggcgt
       61 ttgaggtgga gcgcaccgtc aagagccgtc gccggtatca ggaagtggtg tccgggtatc
      121 tgttcaaccg taaggcgaac ggcatcgatg aaatctggta catctgcccc gaccggccga
      181 cgcttgtccg ggtacagcgg gcgattctgg cggtggatga aatcgtgaac ccgcagaccg
      241 gcgaagcccg caaaacggcg gaactggacc gggagaggct gttcgcgtgc tttaaattta
      301 tgacaacaga gtgagggatt atggcgctgt acatggtttt tattgaggat gtggaagaca
      361 aagacggcga gcgctgcatt atgccaacca gcgagcatct ggaagacatg gcggtcatgg
      421 cctggcagac cggggcatat gtattccggt ttgaagatca ggcccagcat ctggcgatga
      481 tgaagtttat cggcatgtat cacgaacggc tgaacagcga tgacaaagcc acgtattact
      541 tcaccggcaa aaatccggcg tgggaacgtg ataaaccgtc cggaatgaag ctgtgtgatt
      601 atctttaccg caaggttgag cagttcgcca tcgagaacgg cgtgcaggta ctggacgcag
      661 ggaagtacac cccggagcag atcggcgcga tgattgacgt ggtgagtgag cggcacggtg
      721 agagtgcggt gaacgagaag ctgaaagcgg agctgatgcg agagtataac agccggtatt
      781 ttaacgagcc ggtgcccgta ttgccggagg ataaacccgc ccgccggtgg tggccgttcg
      841 gcaaaaaata aacgccagca ccggccatgc cggtgctgtt attcttcccc gagcttaccc
      901 cgcattacca gccagagttt ttccgcatcc tcgtgaagtc gttcgcacat gtcggccggg
      961 tcgtccatgt ccagctcgtt cagcttttcc agcaacagca gcgactgatc cctgatgaat
     1021 tttgccatgt tgagggcggt tttttcctgc ttcgtcatga ttttttccct tccgggcatg
     1081 gtgtgcgcca gtcgggtata ctgtctcaaa aatagcgggg tgagacaatg gcgtgggtga
     1141 cggtgagaca ggcgacggaa ctgacgggga aagcccggag ttccctgtac cgggatatgg
     1201 cgaaaggccg cgtatcatac cggacggagg ctgatggcgg tcgggttgtg gataccagcg
     1261 agcttatccg ggtgtacggt gagttaagac agggtgagac tcacgagcgg gacgggttga
     1321 gactgccaga tgagactgaa aaaacggcct ctgaagcgtt gatcgcggag ataaaagcac
     1381 tcagggagga ggtggccggg ttgcggcagg aaatgcaggc catgagactg ctggaacaca
     1441 aaccggagac cgtagagaca ccggccgaaa cgccgcgccg gtggtggcat tggggaaaac
     1501 gctgacgcgc tgcgcttgtc caaccggcga cgggcacgca atgaattgcg cccctgtcac
     1561 cggttattag cgaacggtat ggggattctg gcgctggccg accacggcca gaacccggac
     1621 tttctgcgcg gggtcgtcca cttcatagag gacatgttgt cccatcaggc tgatccggcg
     1681 aacgtcttcg ccataacgtg agtccacttc gtagaggcgc gggaagtccg ccagtttgtc
     1741 cgcagcagcg agaacccggt cggtaacgtt ctgcgcatcc cacagcgatg aattttccgc
     1801 gatatagtca aacagtgtcg ttacatcttc gatggctccc tgcttccatt ccacgtcata
     1861 cggcatcgcg cagttcctga taacggccat gcaggcgctg gcggagttgt tccgtggtca
     1921 tgcccggacg cgtgtcagca cgggcggcgg tcactttggc gcgaacctga tcttcatact
     1981 ctgcctgggc cagttcttcg ttagccaggc gctgcaacag cgcagctttg tccgggccac
     2041 cttccggcag tatgcggggc aggctttcag ccagttcccg aagaaagttg gcgttttcgt
     2101 agtgtgtcgt gttgctcatt gcggagcctc ccgggttaat gcggcaatat gaaaaagttt
     2161 agcaggaggc ggcctgaaag aatatgctcg tgtcagtcag gctgatgaag cagaaagtcg
     2221 gtaaaaaagg gcttacggca tccattttac gtcaaatccc atgccatgca gttacgtgca
     2281 tagccatgcg ttcaggtaca atttatccgg cgcgtttccg ggggtgttgc gggtggtttg
     2341 ttgtctgttt tatcggtttc ccgtcagaaa tgccctcaga ggctctcagg gggtgcgggc
     2401 aacggatgtt atggtaaatg acgggattgt gaggcatcca gctgacgatt tttgcgtcaa
     2461 cttttcgctg tatgtttaaa catgagttta acattagcgt catctgatag cgctgttgct
     2521 aatttcatct acccgacagg taatttcctt gtaaatcaag cgcgaaagat cagcaaaaaa
     2581 gctatgtgtg cgcttatgga atagggtaga ataagggatt ctgatgatcg tgaggttgtc
     2641 agtgaaaaac gaggcagcta tcagatcttc ggcctgagaa actaaggatt ctgatagctg
     2701 ccggtgactc agttcgcgaa aactgaatct tacctgatca acaaacgccc gattagggcg
     2761 tttgtcaagt ggtgctgcat tatgtagtaa cttggtaccg acttgccagg catttttcac
     2821 agttttttga tgtgcgaagg ctcatccgga caccgtcacc tgttatttat cctctgtaaa
     2881 caggtgctat aaatgcaaaa agtaaccgat ctgtatcctc ctgaaattgc tcgccataag
     2941 ctccagaatc acttcgccgg taataccgtt atgctggagt taatccagaa actaaataaa
     3001 accagcctgt gtacttttgc ggcgctctgt gacggcaacg tcgtgaccac ttccggctat
     3061 aacattacgg ccgatctgtg tgttaaccgt gcgtcagccg tggcccattc gcttaagcaa
     3121 aaatatctgc cggttacggc cagaacgatt tcgactaagg ccgatgtagg tggtgcggta
     3181 aagcaggcgg ctttctgtat cgatgaagac gatcttgcaa ggctgaaatc agagccggaa
     3241 aagatgatga aagagtgcga acgtaacctg aacagccaga agcgaaccaa tgctcagaag
     3301 gaaatgagtc ggttgtacaa ggagtttggt gaagacggca tactggcgct gttacgcaat
     3361 gttgcccggt cgaacggtac accgccgcca ggcgcacacc ctgcaagctg ataccagtaa
     3421 agcccctcag gggctttatt tttaaggttt tctggatgcc gccagctctt catctgtcag
     3481 ctgatctcgg tgcagatagt tcccttttac cggcggtggt gtcaccagtt ttgctttgcc
     3541 ttctttcgga tcgtatatcg ccacaaggcc ggtaatgctt ccttcctcag tcagtgccca
     3601 tgcagcaagc tgataaacga tcaccggata atcagactgg tcggtaaaat gacgaaaata
     3661 ccagtcctgt gcggggatga gttgcttaaa gttcatgtat tagctccttc accattccgc
     3721 ttttgtgtct gcaatgacat agggtttcca cgtcggtgat ttacacgggc tttccgggta
     3781 atgtggcgga aatgtattgt ctctggagtg ggcatttacc gtcacttcac gattacattt
     3841 cgtgcatttg tagatccccg agtgtggaac ttcatctccg gctgaaaatc gcttaccgaa
     3901 gaagtcatcg ctggcgtttg ttgccagttc attcagcgtt tttttctgta ctaaggccat
     3961 tctgctttcc ttcccatcga tatttttcgg gcattcttac ccattttata aatgtggtct
     4021 ttgtggcgtt ttttcaagcg gctgagtttt ggttaagtca ggattatgag tatgatggcg
     4081 tgattttaac catttaaagt acaagaaatt agtatggaga agcagtacac caatgaactg
     4141 accgcggaga ttctggccgg aatggatcaa tcacctttta ctcctgaaca actggctgcg
     4201 atgagcgatg aagcacgcgc gttgattgag gagcaggagg cattctgcca tgcgcatccg
     4261 gtgacgacca tttaccgact ggcggtggcg ggctgtctga cccggcgcgg tggtaccggc
     4321 gatgagttca atccgaaccc tgaggaaggc cataaaatcc ggctggagaa cgggttgtgg
     4381 gtcagcgtgc tgactgaagg ttgtaccgtg acgtatcctg atggcacgca agcccggatt
     4441 ctgtaggatc ttaaaaagat ccacttgaga tcgtttacca ctgcgcgtaa tctcttgctc
     4501 tgtaaacgaa aaaacccgcc gtaaggcggg ttagttcggg ttcgtcgagc ggcaaactcg
     4561 ccgaaaccgg gcggtaactg atcttcgcag gaacagtcac caaaacagta cagtcagttt
     4621 agctgcttca aactggcttc ttcaagcgaa aaagttactt tttcactctt acgttcctgc
     4681 gaactccagt taccagtggc tgctgccagt ggcgttttac cgtgcttttc cgggttggac
     4741 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta
     4801 cagcccagct tggagcgaac gacctacacc gagccgagat accagtgtgt gagctatgag
     4861 aaagcgccac acttcccgca agggagaaag gcggaacagg tatccggtaa acggcagggt
     4921 cggaacagga gagcgcaaga gggagcgacc caccggaaac tgtggggatc tttaagtcct
     4981 gtcgggtttc gcccgtactg ccagattcat ggttgagcct cacggctccc acagatgcac
     5041 ctaaaaagcg tctgtttatg tgaactctgg caggagggcg gagcctatgg aaaaacgcca
     5101 ccggcgcagc ttcgtattat tttctctcta tgcctgtttg ccctgctcgt gagtccgcag
     5161 cgcccgccgc agtttcacgg ccggagcgta gcgaccgagt gagcgaggag gcgcaatatc
     5221 tcccgttttt gcctacggcc accggacgcg ctgatgtgac attttgctcc tgattaaagc
     5281 ctttctgttc cctgtcgata aatcatcagg ataggtgaag tatgtcggtt ttctgataca
     5341 cattttacgt taacctatct ctgtttcttt ttctccttgc ggagaaatgc aagtcatccc
     5401 cgctagggcg ggactgactt caggggtaaa tcccctgctt acgctggctg aacgccaccg
     5461 acgcagcgtc tttacccctc tttctgtctt agcccggctg gacgccggtc acgccagaaa
     5521 ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg
     5581 cacctgacga agcagcaaag ctgctcctga ttttatgggt ggtcagccgc tcgctgtcgc
     5641 ttactctgac gggggccgga acaccggaaa agacagcact ggggtgccgc cttttcctct
     5701 cgcacgctgt gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga
     5761 aagaggctgg cctccgtggc gagcgctgaa cgctttcacc acggacgtga acgccgccgc
     5821 catgccttgg ggcatgtcgt cagcttgcgc agatgggctg cgcatcacgc cggtaaatcc
     5881 gtcgtctgta ggagagaggt tgcatgttga atacggattc gcttgcctgg cagtggcata
     5941 tcccggagct ggcccggcag atttacccgt gggtcagctg gctgtaccgg gagtattacg
     6001 gtctggccgg ggcttttctg gttatcggcc tgtttttcgg tctggctgaa ctctgggccg
     6061 gacggatgcg ttttgtgggc ctgagccata aggagcggcg ggagtatgcc gatcgctata
     6121 agcgcgataa tgacagggct gcgccatttt cggtgctggc ctcgctggat tctcccgggc
     6181 ggatggtcag ggctcgtcga catgtctgga actacgcgta ctggctgacc ggcgctgccg
     6241 gtctggttct gctggggggg ctggttgtcg ccgtgtttat cgagagctgg ggagaaacgg
     6301 tgttgcgtcc gggctactac gcgaatgccg attattacct gctgcaaccc gctaatctgt
     6361 tcgggatagc ctggtgtgcc ggtggtctgg tcgtcgggat acttctggcg cggctactgg
     6421 cattcctgct gcttgacggt cattttgcgg ccgctgacga ggcggtcaat gcaaaactga
     6481 atcaggagag ccggcgcagc agccagcgca ccggcgaaat gactgacgtg cgccacctgc
     6541 atttcgggga gccggtgccg gtcaacgcgc tggccgattt ttccacggag caggcccggc
     6601 agcagcaggt ggtgttcctg ggtaaggacg aggacggcca gccggtgcgg gttccgcgcg
     6661 aggcgtggcg caagaccaat attcagatcc tcggcctacc cgggagcggc aagagcgtga
     6721 tggccaccaa cgcgctgatc cgctgcgtgc gcgattttgg cgatgcggtg gtgtacttcg
     6781 acccgaaagg cgacgcatgg gcgccacacg tctttcgggc gcactgcccg gacttcaccc
     6841 tgctggacct gcggccgggg aaaccggcgc agctgaacct gttccgcgac cttgaccagt
     6901 acgcactgaa aaatctgctg gttgccgggt ttaacctggc ggaaaccggc gatgtggccg
     6961 accactaccg catcagcgag cagaaagcgg cgaagcttat cgcggagcag ttcccgcacg
     7021 gcgcgaacat tcagcaggtg ctggcggcgg cgtatgcgct gccggaggcg ctgaaaaagg
     7081 acgtgaaggg gctgatcacc aagctggaga acgtcgcgga cctgagcgtc ctgcagacgg
     7141 attccggtat tgatgtggcg gggattatca acggcggcgg ctgtctgtac gtcatcggat
     7201 cgatggacga cgaggcggtg atccgggtgc agaaaatgct gtttgcccgc tgcgcgcaaa
     7261 tcatcattgc gcgggacgag ttccgcgcgt ggccgcacgt cagcgtgatg ctcgatgaaa
     7321 tcaaatatct gctctcgaag tacgtactca acgcgctggg gacgctgcgc agccgggact
     7381 gtaacctgct gctggctcac cagtcgctgg gcgacttcgg ccagtgcggg caggatctgc
     7441 ccgctgattt tgtgaaaacc acggtactgg ataacacgcc aatccgctgg ttctatcgcg
     7501 cggccagcca ggagtccgcg cagtgggcgg ccggacagac cggggaaatc cgggtggacg
     7561 tggagcgccg ccgggccagc cgggaggcgg ggaacgtgga gcacatcagc ggggacagct
     7621 tcatccagaa ggaagcgcgg ccgctgttcg acgtgaacac cctgcaacac ctgccggacg
     7681 ggttcgcggt catgaccggg ctgggcgtgg cgcggctggc gttcagcagc ccgctgcggg
     7741 ttgatcgccg ggaaattccg ctgaaaacct tcccggtgct ggcgaaaacg gacccgctgg
     7801 cggagtacca ggctgaggca gtgcggcagc gcccgggtga cgatgatttt gcggggctgt
     7861 actgatggct ctggagcaat acaacgtcag ccacgcgaag cgacaggcgc ggaacgcgga
     7921 gaaaacccgg ctagtcctgc ggtggctgcg ggacgaactc tgctcgacgg ctgaaaccgt
     7981 agcccgtctg ctggggatcg gggcggtgca gccggtttac cgctttctgg acagcctggt
     8041 ggcgaaagga ctgctggccc gggcgaaata cccggttgac ggtcgtcagg tcagcgtgtg
     8101 ggggctgacc ccgcacggcg tggcgttctc gttcgacgag gacgagccgc tgacggacgt
     8161 tattccgttt cagccctcgc gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca
     8221 gtccctgcgt cttgatatgg aagccagagg ggccaccggc tggcgcaccc tgcaccgcct
     8281 ga
//
