LOCUS       NZ_CP110905             9380 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain CF2206 plasmid pCF2206-5, complete
            sequence.
ACCESSION   NZ_CP110905
VERSION     NZ_CP110905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693662
            Assembly: GCF_026240855.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 15:46:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,545
            CDSs (total)                      :: 5,423
            Genes (coding)                    :: 5,294
            CDSs (with protein)               :: 5,294
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 33 of 129
            Pseudo Genes (incomplete)         :: 86 of 129
            Pseudo Genes (internal stop)      :: 26 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110905.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CF2206"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCF2206-5"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2022-06-23"
                     /collected_by="zhangxiaofan"
     gene            join(8884..9380,1..1579)
                     /locus_tag="OQY67_RS27495"
                     /old_locus_tag="OQY67_27490"
     CDS             join(8884..9380,1..1579)
                     /locus_tag="OQY67_RS27495"
                     /old_locus_tag="OQY67_27490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_265970842.1"
                     /translation="MQTELIKELNKVLKVFPQFWSGENLHRSMVSDAISQKQPDLIKAL
                     VANKKIRSVYGTDINGTLIFDFDKLCSLLKYKEYWENSFTKYRNKIGLTSSGKYIADST
                     DIVLDFPFKDCLLEGGMTKEEQGKDEVYYNEIIARDEIDNLLSPKALVNVSRHTKDGEV
                     KNIHSFHEKDNLIIKGNNLIALHSLLGKFTGKVKLIYIDPPYNTGSDSFKYNDNFNHSS
                     WLTFIKNRLEVAKELLSEDGVIFVQSDDNEQAYLKVLMDGIFDRENFINMISVNMKNVA
                     GASGGGEDKRLKKNCEFILIYSKNYASLSAFKNVFEYTEIHEVVEQYRKEGKNWHYSSV
                     LVNAGEKIYVTSTVDGSGNEIKIYKHPNAVIKSVRQLMAQENISEKDVFYNFGTKIFEA
                     KDAQSSIRTRVAKAKKEHHVNDDILSIEYTPKSGKNKGQVYEQFYKGDKCRLFAWLRDI
                     TENIDGILYKKDKQGTYWDFTSHMNNLMKEGEISFQNGKKPEALIQRIIDISTNKDDIV
                     LDFHLGSGTTAATAHKMERRYIGIEQMDYINDITVPRLKNVIAAENNGISRDVSWQGGG
                     SFVYAELMELNAYFVHEIQKAQSTEELEKLFAVMKTEAHLNYQVALENVLSAEYEMDGI
                     PRKVAFSELELHEQKQLLIDILDKNQLYVNVSDMDDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            1584..4220
                     /locus_tag="OQY67_RS27500"
                     /old_locus_tag="OQY67_27495"
     CDS             1584..4220
                     /locus_tag="OQY67_RS27500"
                     /old_locus_tag="OQY67_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_266020241.1"
                     /translation="MAKKPKSAGSEKLLFNKLKQFDEPGLFQDSYQTPDYIQANLKDAL
                     RPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSQELEINGEKITIAPVDTFPSAPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKAKGKSKTSPENEEQTWE
                     RTIENILALRPDNRLFEYTATIDLANKDIGNKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDSEKMLDAVLLSQYRKLIAIDNGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESVRRHLANKRVQLSSDTSIWHKVIQRYAGSDLVTVIGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTGTDSEAQL
                     IGRGARYFPFVYDGKRSFTRRFDTSAKELSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTIEHARLKEDFKKSSVYQTGKLYFNEVEEIESSSRNWETYSLETRFEIPYQT
                     AGEESLDNLTGATAGITKSEPLLLDERFYRKAMQRISFYALDNLQRLFPKLTGIREFIR
                     SESYLGKLKITVTVPQSLDLAIVPAKEKLHLLETVLLRIAENMRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVVINQKITAVPTIGKKWYVYDNAILNQLEHRLVKTLEAFIPKL
                     RARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTCHADNAYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNEGESLET
                     ASLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(4263..4847)
                     /locus_tag="OQY67_RS27505"
                     /old_locus_tag="OQY67_27500"
     CDS             complement(4263..4847)
                     /locus_tag="OQY67_RS27505"
                     /old_locus_tag="OQY67_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_152954525.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVISLTEQFQDASAQLQQQSETLSR
                     LIRELDSASGNMAETVRKSVNSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(4860..5957)
                     /gene="mobA"
                     /locus_tag="OQY67_RS27510"
                     /old_locus_tag="OQY67_27505"
     CDS             complement(4860..5957)
                     /gene="mobA"
                     /locus_tag="OQY67_RS27510"
                     /old_locus_tag="OQY67_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_265970836.1"
                     /translation="MASYHLSVKTGGKGRASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQKERER
                     QQEKERQRLAEHTQQKPDKGWSFSR"
                     /name="mobA"
     gene            6122..6589
                     /gene="traD"
                     /locus_tag="OQY67_RS27515"
                     /old_locus_tag="OQY67_27510"
     CDS             6122..6589
                     /gene="traD"
                     /locus_tag="OQY67_RS27515"
                     /old_locus_tag="OQY67_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_266020240.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLIMAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            7185..8141
                     /locus_tag="OQY67_RS27520"
                     /old_locus_tag="OQY67_27515"
     CDS             7185..8141
                     /locus_tag="OQY67_RS27520"
                     /old_locus_tag="OQY67_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_208460129.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYASFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDDFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELTKDPISKKEDDSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            8149..8598
                     /locus_tag="OQY67_RS27525"
                     /old_locus_tag="OQY67_27520"
     CDS             8149..8598
                     /locus_tag="OQY67_RS27525"
                     /old_locus_tag="OQY67_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_208460128.1"
                     /translation="MALVSISEAARLTGKSRTTVQAYIKQGKLTKCTDSAGASKLDTSE
                     LLRVFGSFTGQQVGSEQSSIIVQHLAGESVQLTDQNLLHENYALKAEVELVKALLREKE
                     LLLNEKDKRNEDLKQALLLIESKLPATPKPATQPVEKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     Typing          7462..8141
                     /name="rep_cluster_1787"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4864..5957)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.041"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcatagcttc catgaaaaag ataacttgat aataaaggga aataatttaa ttgcacttca
       61 ctcactattg ggtaagttta ctggaaaagt taaattaata tatatagacc caccatacaa
      121 tacaggatct gacagtttta aatacaatga caatttcaat cattcatcat ggctaacatt
      181 tataaaaaat agactagaag tagcaaaaga acttcttagt gaagatggag tcatcttcgt
      241 acaatcagac gacaatgaac aagcgtattt aaaagtttta atggatggca tttttgatag
      301 ggaaaacttt atcaatatga tatcggttaa catgaagaat gttgccggag cctctggtgg
      361 aggtgaggat aaaagactta aaaaaaactg tgagtttatt ttaatttact caaaaaacta
      421 tgcctccctt tctgcattta aaaatgtttt tgaatacaca gaaattcatg aggtagttga
      481 gcaatatagg aaggaaggta aaaattggca ctattcctcc gtactggtaa atgcagggga
      541 aaaaatatac gtaacctcta ctgttgatgg cagtgggaac gaaataaaga tatacaaaca
      601 ccctaacgca gtaattaaat cagttaggca attgatggcg caggaaaaca tttcagaaaa
      661 ggatgttttt tataactttg gaacaaaaat atttgaagcg aaagatgctc aatcatccat
      721 tagaacgcga gtagcaaaag caaaaaaaga gcatcatgtt aatgatgata tattatcaat
      781 agaatacaca cctaaatcag gaaaaaataa aggtcaagta tatgaacaat tctacaaagg
      841 ggataaatgt agattgtttg catggcttag agatataact gaaaatatag atggcatttt
      901 atacaaaaaa gataaacaag gaacgtattg ggatttcaca tcacacatga acaatctaat
      961 gaaagaaggt gaaatatcat ttcaaaatgg aaaaaaacca gaggcattaa tacaaagaat
     1021 tatagatatt tcaacaaata aagatgatat tgtactagat ttccatctcg gaagcgggac
     1081 aacagctgca acagcacata aaatggaacg tcgttacatt ggcattgaac aaatggatta
     1141 catcaatgac ataacggttc ctcgactaaa aaatgtaatc gcagcggaaa acaatgggat
     1201 ttcaagagac gtgagctggc agggcggtgg aagctttgta tacgccgaac tgatggagct
     1261 gaacgcgtac ttcgtgcacg aaatccagaa agcgcagtcc actgaagaac tcgaaaaact
     1321 ctttgctgta atgaaaaccg aggcgcatct caactatcag gtggcactgg aaaacgtact
     1381 ctctgctgaa tacgagatgg acggcattcc gcgtaaagtt gcgttcagcg aactggaact
     1441 gcatgagcag aagcagctac tgattgatat cctcgataaa aatcagctct acgtgaacgt
     1501 ttctgatatg gatgacagtg acctgaacat ctcagagagc gataaagcct ttacccgcag
     1561 cttctacgga atggagtaac ggcatggcga agaaaccaaa gtcagccggc agcgagaagc
     1621 tgctgtttaa caagctgaag cagttcgatg aacctgggct tttccaggat agctaccaga
     1681 cgccggacta cattcaggcg aacctgaagg atgccctgcg cccgtaccag catggggcgc
     1741 tgcgctacct gcattacact cagcgcaaac gtgaagaggc gctgctgcat taccgccatc
     1801 tgttgtttca tatggcaacc ggtgcaggga aaacgatggt gatggcgggg acgatactct
     1861 atctgtttaa agagtacggg tatcagaact tcatcttttt cgtgcatacc gacgcgatta
     1921 tccagaaaac ccgagagaac ctgctcaacc cgcagtcgcc gaagtacctg ttcagtcagg
     1981 agctggaaat caacggggaa aaaatcacca ttgcaccggt ggatacgttc ccgtctgcac
     2041 cagagcgcaa cacgatttac ctcaagttat caactatcca taagatgcac gacgagctga
     2101 acagctatcg tgagaacagc atcacctacg aggatttaaa agaaataccg ctcgtcctgc
     2161 ttggcgacga ggcgcatcac ttcaacgccg gcacgaaagc gaaaggcaag tcaaagacct
     2221 caccggagaa cgaggagcag acctgggagc gcacgattga gaacatcctc gctctgagac
     2281 cggataaccg actgttcgag tacaccgcca ccattgacct ggcaaacaaa gatatcggaa
     2341 ataaataccg tgacaaagtg gtgtatcagt atgacctgaa gcagttcatg tcagacggtt
     2401 actcgaaaaa ggtcatgcta ctggaagcca accagaacga cagcgaaaaa atgcttgatg
     2461 ccgtgctgtt aagtcagtac cgcaaactga tagcgataga taacggcatc accggcttca
     2521 agccggtcat tctgttcaaa tcgaacaaga ttgcgatatc gaaggcgaag caggaagagt
     2581 tttcgcaact gattgcggca atgacccctg aaagcgtcag acgtcatctg gcgaataaaa
     2641 gagtccagct cagctctgac accagtatct ggcataaagt gattcagcgt tacgctggca
     2701 gcgatttagt gacggttatc gggcagattc aggaagattt taacgacttc aacctgctga
     2761 acgtcaataa atccgacttg ctggaagaaa accccgtcct gctgaatacg ctggaagaag
     2821 tcgataatcc ggtgagagcc gtctttgccg tggcgaaggt caatgaaggc tgggacgtgc
     2881 tcaacctcta cgacatcgtc cgcatcagcg aacaggcgag tagcagcaaa accggcacgg
     2941 acagcgaggc acagcttatc gggcgcggcg cgcgttactt cccgttcgta tatgacggca
     3001 agcggagctt cacccgccga tttgatacca gcgcgaagga gttgagcgtg ctggaacaac
     3061 tgcactatca caccatcaat gaaccggcgt acatcaaaac gctgcacgca tctctggagc
     3121 aggcggacat tgacgtgcat caggacggcg gcggcacgat tgagcacgcc agactgaaag
     3181 aggactttaa aaagtcctcc gtatatcaga caggtaagct gtactttaac gaagtggaag
     3241 agattgagag cagcagtcgc aactgggaaa cctactcgct ggaaacgcgc tttgagatac
     3301 cgtatcagac cgccggtgag gagtcgttgg acaacctgac aggggcaacg gctggcatca
     3361 caaagtcaga acccctgcta ctggacgagc gtttttatcg caaggccatg cagaggatca
     3421 gtttttacgc gctggataac cttcaacgcc tcttcccgaa actgaccggt attcgtgaat
     3481 tcatccgcag tgagtcttat cttgggaaac tgaaaatcac cgttaccgta ccgcagtctc
     3541 tggatttagc catcgtaccg gcaaaagaga aactgcacct gctggaaacc gttctgctgc
     3601 gtatcgcaga gaacatgcgc cgcaatgacc agaaagtgaa aggtacgtac cgttttatca
     3661 gccagccggt gaaagaggtg attaaagact acagcctgca cattgacccg tcggtagtca
     3721 ttaaccagaa aatcaccgcc gtacccacca ttggtaaaaa atggtacgtg tacgataacg
     3781 cgatactgaa tcagcttgag caccgtctgg tgaaaactct ggaagccttt ataccgaagc
     3841 tcagagcacg ttacgacgat atttacgtgc tgcgtaacga cgagcagtca acgcgcttta
     3901 agctcacgga atttggcggc gtacgtggct ttatgccgga cttcatcatg atactgacgt
     3961 gccatgcgga taatgcttac tggcaggtct tccttgagcc gaaaggggat gaccgtctac
     4021 tggatgacgc atggaaagag cggatgctcg aaaccctgaa tgaccgggaa cgcattgtaa
     4081 ttgatgaaaa tgaggacgtc cggctggtcg ggattaaatt ctttgccaac agtcagatgg
     4141 atgccttcgt cagtgatatg cagaaccggc tgaatgaggg ggaatcgctt gaaaccgcat
     4201 cgctctccct gccactgtaa acggtgacaa atacaaataa cggcgcacag tgcgccgtta
     4261 ttttattttt tgataatcat ccaggtattc tctttatcac catacgtggt gcgagtgtcc
     4321 accttaacgc agggctttcc gtcacaacgc gatatatcag ctagcggggc tttcttctgc
     4381 cagacggctt ccatccgctg catttcagca atctggctgt acccgtcatt cataaaccac
     4441 gtgaatgccg tcacgcaggc agccagaacg aagaatatcg tcagcacaag ataaatcgcg
     4501 gatttccacg tatagcgcga catctcgcgg cgcatttcat ggatcattgc cttcgccgta
     4561 tcggcagcct gattaagcgc ttctgtcgct ggtttctgct gtgctaatcc ggcttgtttc
     4621 agttctttct caacctgagt gagcgcggaa ttaaccgatt tcctgacggt ttcagccata
     4681 ttaccggacg cgctgtccag ctcacgaatg agcctgctca gcgtttcact ctgctgctgt
     4741 aattgtgctg aggcgtcctg aaactgctca gtcagagaga taacacgctt ttccagcgcc
     4801 tgatgatgcc cgataagggc ggcgatttgt ttaatttcgt cactcatgca aaatcctgtc
     4861 tatcgtgaaa atgaccagcc tttatccggc ttctgttgtg tatgttcggc aagtctctgc
     4921 cgttctttct cctgctgacg ctcgcgttct ttctgtcgtt ccatttcctg atgtatcccc
     4981 tgaaactccg ccattgcatc gttaaccagg gactgaaggt cgatttcttc ctgtatatcc
     5041 ttcatggcat cactgaccag tgcgttcagc ttgtcaggct ctttttcaaa atcaaacgtt
     5101 ctgccggaat gggattcctg ctcaggctct gacttcagct cctgttttag cgtcagagta
     5161 tccctctcgc tgagggcttc ccgtaacgag gtagtcacgt caattacgct gtcacgttca
     5221 tcacgggact gctgcacctg cctttcagcc tccctgcgct caagaatggc ctgtagctgc
     5281 tcagtatcga atcgctgaac ctgacccgcg cccagatgcc gctcaggctc acggtcaatg
     5341 ccctgcgcct tcagggaacg ggaatcaacc cggtcagcgt gctgataccg ttcaaggtgc
     5401 ttattctgga ggtcagccca gcgttccctc tgggcaacaa ggtattcttt gcgttcggtc
     5461 ggtgtttccc cgaaacgtgc cttttttgcg ccaccgcgct ccggctcttt ggtgttagcc
     5521 cgtttaaaat actgctcagg gtcacggtga ataccgtcat taatgcgttc agagaacatg
     5581 atatgggcgt ggggctgctc gccaccggct atcgctgctt tcggattatg gatagcgaac
     5641 tgataggcat ggcggtcgcc aatttcctgt tggacaaaat cgcggacaag ctcaagacgt
     5701 tgttcgggtt ttaactcacg cggcagggca atctcgattt cacggtaggt acagccgttg
     5761 gcacgttcag acgtgtcagc ggctttccag aactcggacg gtttatgcgc tgcccacgcc
     5821 ggcatattgc cggactcctt gtgctcaagg tcggagtctt tttcacgggc atactttccc
     5881 tcacgcgcaa tataatcggc atgaggagag gcacggcctt tcccgccggt ttttacgctg
     5941 agatgatagg aggccatcgt gtttttatcc cgctgaaggc gcgcaccgtt tctgaacgaa
     6001 gtgaagaaac gtctaagtgc gccctgataa ataaaagagt tatcagggat tgtagtggga
     6061 tttgacctcc tctgccatca ctgagcataa tcattccgtt agcattcagg aggtaaacag
     6121 catgaataaa agcgaaaaac aggaacaatg ggcagcagaa agagtgcagt atattcgcgg
     6181 cttaaagtcg ccgaatgagc aacagaaact tatgctgata ctgacggata aagcagataa
     6241 aacagcacag gatatcaaaa cgctgtccct gctgatgaag gctgaacagg cagcagagaa
     6301 agcgcaggaa gccagagcga aagtcatgaa cctgatacag gcagaaaagc gagccgaagc
     6361 cagagccgcc cgtaaagccc gtgaccatgc tctgtaccag tctgccggct tacttatcat
     6421 ggcgggactg gtagacagcc agacagggaa gcccgtcgat gataccgctg ccttactggg
     6481 tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat
     6541 cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca
     6601 taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat
     6661 gaatccataa aaggcgcctg tagtgccatt tacccccatt ccctgccaga gccgtgagcg
     6721 aagcgaactg attttcacag agataacagc gattcaggtg ccggacggtc ggagacaaaa
     6781 ggaatgttca gcgggttgcc cgagcttgcg agggtgctat ctcagtgttt ttgggggtta
     6841 aggtctgttt tgaagagaag caaacagcgt ttgcgacatc cttttatcat atcgctcaag
     6901 tgactcaggc gtgcagttat ccacaaggct gggatctatt cttttttatc tttttctatt
     6961 ctttctttat tctaaagatt ttaagttatt gttaaaaaac aaaaaaaaac aggaaaggtt
     7021 aagcaaaaat taggaaaggt taagcaaaaa ttagtatttc cctcccaaag gttaagtgaa
     7081 aattaggtaa tccacacaac cacgcaaaag gttatgccag tggaattgac ataaccaata
     7141 cgaaaggtta tgttaattta aaatcacaac caatggtttt agtcatggct gaattggtag
     7201 tattcaaagc aaatgagctt gcggttagtc gttatgacct aacagagcac gaaacgaaac
     7261 taatcctgtt ttgtgttgca aagctaaacc ccacattaga aacaccagat gaaactgaac
     7321 gaacggttaa attttcatgc tcagaatatg cttcatttat ggggataagt tatgagaatg
     7381 cgtggggacg cttaaataca tcaacacgag aattatttaa gcggtctatt gagttaatat
     7441 atccaacagg tgctgtatct atgcgtgttt ttaattgggc tgaatatgca gagttcaata
     7501 gggaaacaca agaagtaact ttagtattta gtaaatatat aatcccgttg ctatttcacc
     7561 taaagagatt cattaaatac aacctcgagc atgttaagtc ttttgaaaac aaatattcaa
     7621 tgcgcattta tgaatggcta ctaaaagaac taacacaaag aaaaacacat aaagccaata
     7681 tttcaataag tattgatgat tttaaattca tgctaatgtt agaaaaaagc tacccggaat
     7741 ataaattact aaaccattgg gttttaaaac caatatcaaa agatttaaat acatatagca
     7801 atataaaact atctattgat aagcgcggtc gtcctgctga tacgctgatc tttcaagttg
     7861 aacttgataa acaaattgac cttgtgactg agctaacaaa agatccaata tcaaaaaaag
     7921 aagatgattc aatccgttta acgcctgaaa atcgtctgca tgaggggcta aaaacaacat
     7981 tgcatgatgc tttaactgcg aaaatacaac ttactggatt tgaagctaaa ttcctgagtg
     8041 acatgcagag taagtatgat ctcaacggct catttacatg gctgactcaa aaacaacgag
     8101 ccacgctaga gaaaatttta gccaagtacg gacgaatctg aggtatctat ggctctagta
     8161 tcaataagtg aagcagcaag actaactgga aaaagccgca caacagtaca ggcatacatt
     8221 aagcagggaa aactgaccaa gtgtacagat agcgcaggcg cttcaaaact agacacatca
     8281 gagcttctac gtgttttcgg ttcattcact ggtcagcagg ttggcagtga acaatcaagc
     8341 atcattgtgc agcacttggc aggcgaatct gtgcagttaa ctgaccagaa tttgctacat
     8401 gaaaactatg ctttaaaagc tgaagtagaa ctcgtaaagg cattgttgcg tgaaaaagag
     8461 ctgctactca atgaaaaaga caagcgcaac gaggatctca agcaagcgtt gttactgata
     8521 gagtccaagc tgccagcaac accaaaacct gccacgcagc cggtggaaaa aaaatcatgg
     8581 caattctgga agaaatagag gtttcagcct gtttactggc tgaaaccata cccccgattt
     8641 tgataacaaa ccggtaacac caaaacagcc cattttcggg gattaaaacc cgctattttg
     8701 gacgttccgg cgggttctgg tgatgagtaa caggtcagtg gtgcgcgcaa gatcccttat
     8761 gttaaatacc acctgttttg gtgatttttt ccgcatctgc atcagcagta tcgctgccag
     8821 gtttctgctc aggacgcgat gccctacaat catttcaatc tctaacagtc ataaggtaat
     8881 gacatgcaga ccgaattaat aaaagaactg aacaaggttt taaaagtgtt tccgcaattc
     8941 tggagcggag aaaatttaca ccgttcaatg gtgagtgacg ccatcagcca aaagcagcca
     9001 gatctaatca aagcactggt cgctaataaa aaaatacgtt ctgtctacgg cacagatatt
     9061 aacggaactt taattttcga ttttgacaaa ctttgcagcc tgttaaaata taaagaatac
     9121 tgggaaaaca gctttactaa ataccgaaat aagattggat taaccagctc aggtaaatac
     9181 attgccgata gtactgatat agtgcttgat tttcctttca aggattgcct tcttgaaggc
     9241 ggaatgacaa aagaagaaca agggaaagac gaggtttatt ataacgagat tattgctcgc
     9301 gatgaaatag ataatcttct atccccaaaa gcattggtta atgtatctcg ccatacaaag
     9361 gatggggaag ttaaaaacat
//
