LOCUS       NZ_CP110901             8282 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain CF2206 plasmid pCF2206-6, complete
            sequence.
ACCESSION   NZ_CP110901
VERSION     NZ_CP110901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693662
            Assembly: GCF_026240855.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 15:46:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,545
            CDSs (total)                      :: 5,423
            Genes (coding)                    :: 5,294
            CDSs (with protein)               :: 5,294
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 33 of 129
            Pseudo Genes (incomplete)         :: 86 of 129
            Pseudo Genes (internal stop)      :: 26 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CF2206"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCF2206-6"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2022-06-23"
                     /collected_by="zhangxiaofan"
     gene            join(8202..8282,1..438)
                     /locus_tag="OQY67_RS25550"
                     /old_locus_tag="OQY67_25545"
     CDS             join(8202..8282,1..438)
                     /locus_tag="OQY67_RS25550"
                     /old_locus_tag="OQY67_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704828.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVARSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            complement(469..723)
                     /locus_tag="OQY67_RS25555"
                     /old_locus_tag="OQY67_25550"
     CDS             complement(469..723)
                     /locus_tag="OQY67_RS25555"
                     /old_locus_tag="OQY67_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321408.1"
                     /translation="MNFKQLIPAQDWYFRHFTDQSDYPVIVYQLAAWALTEEGSITGLV
                     AIYDPKEGKAKLVTPPPVKGNYLHRDQLTDEELAASRKP"
                     /name="hypothetical protein"
     gene            complement(736..987)
                     /locus_tag="OQY67_RS25560"
                     /old_locus_tag="OQY67_25555"
     CDS             complement(736..987)
                     /locus_tag="OQY67_RS25560"
                     /old_locus_tag="OQY67_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321409.1"
                     /translation="MALVQKKTLNELATNASDDFFGKRFSAGDEVPHSGIYKCTKCNRE
                     VTVNAHSRDNTFPPHYPESPCKSPTWKPYVIADTKAEW"
                     /name="hypothetical protein"
     gene            1141..1473
                     /locus_tag="OQY67_RS25565"
                     /old_locus_tag="OQY67_25560"
     CDS             1141..1473
                     /locus_tag="OQY67_RS25565"
                     /old_locus_tag="OQY67_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321410.1"
                     /translation="MEKQYTNELTAEILAGMDQSPFTPEQLAAMSDEARALIEEQEAFC
                     HAHPVTTIYRLAVAGCLTRRGGTGDEFNPNPEEGHKIRLENGLWVSVLTEGCTVTYPDG
                     TQARIL"
                     /name="hypothetical protein"
     gene            2931..4892
                     /locus_tag="OQY67_RS25570"
                     /old_locus_tag="OQY67_25565"
     CDS             2931..4892
                     /locus_tag="OQY67_RS25570"
                     /old_locus_tag="OQY67_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4892..5623
                     /locus_tag="OQY67_RS25575"
                     /old_locus_tag="OQY67_25570"
     CDS             4892..5623
                     /locus_tag="OQY67_RS25575"
                     /old_locus_tag="OQY67_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            5630..6160
                     /locus_tag="OQY67_RS25580"
                     /old_locus_tag="OQY67_25575"
     CDS             5630..6160
                     /locus_tag="OQY67_RS25580"
                     /old_locus_tag="OQY67_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6188..6367)
                     /locus_tag="OQY67_RS25585"
                     /old_locus_tag="OQY67_25580"
     CDS             complement(6188..6367)
                     /locus_tag="OQY67_RS25585"
                     /old_locus_tag="OQY67_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6437..6814
                     /locus_tag="OQY67_RS25590"
                     /old_locus_tag="OQY67_25585"
     CDS             6437..6814
                     /locus_tag="OQY67_RS25590"
                     /old_locus_tag="OQY67_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(6876..7175)
                     /locus_tag="OQY67_RS25595"
                     /old_locus_tag="OQY67_25590"
     CDS             complement(6876..7175)
                     /locus_tag="OQY67_RS25595"
                     /old_locus_tag="OQY67_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7165..7428)
                     /locus_tag="OQY67_RS25600"
                     /old_locus_tag="OQY67_25595"
     CDS             complement(7165..7428)
                     /locus_tag="OQY67_RS25600"
                     /old_locus_tag="OQY67_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     Typing          1749..1937
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.529"
                     /coverage="94.0"
                     /evalue="1.1199999999999999e-60"
     Typing          4893..5620
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.12e-141"
ORIGIN
        1 ctggagttaa tccagaaact aaataaaacc agcctgtgta cttttgcggc gctctgtgac
       61 ggcaacgtcg tgaccacttc cggctataac attacggccg atctgtgtgt taaccgtgcg
      121 tcagccgtgg cccattcgct taagcaaaaa tatctgccgg ttacggccag aacgatttcg
      181 actaaggccg atgtaggtgg tgcggtaaag caggcggctt tctgtatcga tgaagacgat
      241 cttgcaaggc tgaaatcaga gccggaaaag atgatgaaag agtgcgaacg taacctgaac
      301 agccagaagc gaaccaatgc tcagaaggaa atgagtcggt tgtacaagga gtttggtgaa
      361 gacggcatac tggcgctgtt acgcaatgtt gcccggtcga acggtacacc gccgccaggc
      421 gcacaccctg caagctgata ccagtaaagc ccctcagggg ctttattttt aaggttttct
      481 ggatgccgcc agctcttcat ctgtcagctg atctcggtgc agatagttcc cttttaccgg
      541 cggtggtgtc accagttttg ctttgccttc tttcggatcg tatatcgcca caaggccggt
      601 aatgcttcct tcctcagtca gtgcccatgc agcaagctga taaacgatca ccggataatc
      661 agactggtcg gtaaaatgac gaaaatacca gtcctgtgcg gggatgagtt gcttaaagtt
      721 catgtattag ctccttcacc attccgcttt tgtgtctgca atgacatagg gtttccacgt
      781 cggtgattta cacgggcttt ccgggtaatg tggcggaaat gtattgtctc tggagtgggc
      841 atttaccgtc acttcacgat tacatttcgt gcatttgtag atccccgagt gtggaacttc
      901 atctccggct gaaaatcgct taccgaagaa gtcatcgctg gcgtttgttg ccagttcatt
      961 cagcgttttt ttctgtacta aggccattct gctttccttc ccatcgatat ttttcgggca
     1021 ttcttaccca ttttataaat gtggtctttg tggcgttttt tcaagcggct gagttttggt
     1081 taagtcagga ttatgagtat gatggcgtga ttttaaccat ttaaagtaca agaaattagt
     1141 atggagaagc agtacaccaa tgaactgacc gcggagattc tggccggaat ggatcaatca
     1201 ccttttactc ctgaacaact ggctgcgatg agcgatgaag cacgcgcgtt gattgaggag
     1261 caggaggcat tctgccatgc gcatccggtg acgaccattt accgactggc ggtggcgggc
     1321 tgtctgaccc ggcgcggtgg taccggcgat gagttcaatc cgaaccctga ggaaggccat
     1381 aaaatccggc tggagaacgg gttgtgggtc agcgtgctga ctgaaggttg taccgtgacg
     1441 tatcctgatg gcacgcaagc ccggattctg taggatctta aaaagatcca cttgagatcg
     1501 tttaccactg cgcgtaatct cttgctctgt aaacgaaaaa acccgccgta aggcgggtta
     1561 gttcgggttc gtcgagcggc aaactcgccg aaaccgggcg gtaactgatc ttcgcaggaa
     1621 cagtcaccaa aacagtacag tcagtttagc tgcttcaaac tggcttcttc aagcgaaaaa
     1681 gttacttttt cactcttacg ttcctgcgaa ctccagttac cagtggctgc tgccagtggc
     1741 gttttaccgt gcttttccgg gttggactca agtgaacagt taccggataa ggcgcagcag
     1801 tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac ctacaccgag
     1861 ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgcaagg gagaaaggcg
     1921 gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg agcgacccac
     1981 cggaaactgt ggggatcttt aagtcctgtc gggtttcgcc cgtactgcca gattcatggt
     2041 tgagcctcac ggctcccaca gatgcaccta aaaagcgtct gtttatgtga actctggcag
     2101 gagggcggag cctatggaaa aacgccaccg gcgcagcttc gtattatttt ctctctatgc
     2161 ctgtttgccc tgctcgtgag tccgcagcgc ccgccgcagt ttcacggccg gagcgtagcg
     2221 accgagtgag cgaggaggcg caatatctcc cgtttttgcc tacggccacc ggacgcgctg
     2281 atgtgacatt ttgctcctga ttaaagcctt tctgttccct gtcgataaat catcaggata
     2341 ggtgaagtat gtcggttttc tgatacacat tttacgttaa cctatctctg tttctttttc
     2401 tccttgcgga gaaatgcaag tcatccccgc tagggcggga ctgacttcag gggtaaatcc
     2461 cctgcttacg ctggctgaac gccaccgacg cagcgtcttt acccctcttt ctgtcttagc
     2521 ccggctggac gccggtcacg ccagaaattc acccggcccc cgtcaggctc acgcctgacc
     2581 acccacaaaa atccacggtg ctggacgcac ctgacgaagc agcaaagctg ctcctgattt
     2641 tatgggtggt cagccgctcg ctgtcgctta ctctgacggg ggccggaaca ccggaaaaga
     2701 cagcactggg gtgccgcctt ttcctctcgc acgctgtggg cgtgctcgtt gttcctccgg
     2761 ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgtggcgag cgctgaacgc
     2821 tttcaccacg gacgtgaacg ccgccgccat gccttggggc atgtcgtcag cttgcgcaga
     2881 tgggctgcgc atcacgccgg taaatccgtc gtctgtagga gagaggttgc atgttgaata
     2941 cggattcgct tgcctggcag tggcatatcc cggagctggc ccggcagatt tacccgtggg
     3001 tcagctggct gtaccgggag tattacggtc tggccggggc ttttctggtt atcggcctgt
     3061 ttttcggtct ggctgaactc tgggccggac ggatgcgttt tgtgggcctg agccataagg
     3121 agcggcggga gtatgccgat cgctataagc gcgataatga cagggctgcg ccattttcgg
     3181 tgctggcctc gctggattct cccgggcgga tggtcagggc tcgtcgacat gtctggaact
     3241 acgcgtactg gctgaccggc gctgccggtc tggttctgct gggggggctg gttgtcgccg
     3301 tgtttatcga gagctgggga gaaacggtgt tgcgtccggg ctactacgcg aatgccgatt
     3361 attacctgct gcaacccgct aatctgttcg ggatagcctg gtgtgccggt ggtctggtcg
     3421 tcgggatact tctggcgcgg ctactggcat tcctgctgct tgacggtcat tttgcggccg
     3481 ctgacgaggc ggtcaatgca aaactgaatc aggagagccg gcgcagcagc cagcgcaccg
     3541 gcgaaatgac tgacgtgcgc cacctgcatt tcggggagcc ggtgccggtc aacgcgctgg
     3601 ccgatttttc cacggagcag gcccggcagc agcaggtggt gttcctgggt aaggacgagg
     3661 acggccagcc ggtgcgggtt ccgcgcgagg cgtggcgcaa gaccaatatt cagatcctcg
     3721 gcctacccgg gagcggcaag agcgtgatgg ccaccaacgc gctgatccgc tgcgtgcgcg
     3781 attttggcga tgcggtggtg tacttcgacc cgaaaggcga cgcatgggcg ccacacgtct
     3841 ttcgggcgca ctgcccggac ttcaccctgc tggacctgcg gccggggaaa ccggcgcagc
     3901 tgaacctgtt ccgcgacctt gaccagtacg cactgaaaaa tctgctggtt gccgggttta
     3961 acctggcgga aaccggcgat gtggccgacc actaccgcat cagcgagcag aaagcggcga
     4021 agcttatcgc ggagcagttc ccgcacggcg cgaacattca gcaggtgctg gcggcggcgt
     4081 atgcgctgcc ggaggcgctg aaaaaggacg tgaaggggct gatcaccaag ctggagaacg
     4141 tcgcggacct gagcgtcctg cagacggatt ccggtattga tgtggcgggg attatcaacg
     4201 gcggcggctg tctgtacgtc atcggatcga tggacgacga ggcggtgatc cgggtgcaga
     4261 aaatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggacgagttc cgcgcgtggc
     4321 cgcacgtcag cgtgatgctc gatgaaatca aatatctgct ctcgaagtac gtactcaacg
     4381 cgctggggac gctgcgcagc cgggactgta acctgctgct ggctcaccag tcgctgggcg
     4441 acttcggcca gtgcgggcag gatctgcccg ctgattttgt gaaaaccacg gtactggata
     4501 acacgccaat ccgctggttc tatcgcgcgg ccagccagga gtccgcgcag tgggcggccg
     4561 gacagaccgg ggaaatccgg gtggacgtgg agcgccgccg ggccagccgg gaggcgggga
     4621 acgtggagca catcagcggg gacagcttca tccagaagga agcgcggccg ctgttcgacg
     4681 tgaacaccct gcaacacctg ccggacgggt tcgcggtcat gaccgggctg ggcgtggcgc
     4741 ggctggcgtt cagcagcccg ctgcgggttg atcgccggga aattccgctg aaaaccttcc
     4801 cggtgctggc gaaaacggac ccgctggcgg agtaccaggc tgaggcagtg cggcagcgcc
     4861 cgggtgacga tgattttgcg gggctgtact gatggctctg gagcaataca acgtcagcca
     4921 cgcgaagcga caggcgcgga acgcggagaa aacccggcta gtcctgcggt ggctgcggga
     4981 cgaactctgc tcgacggctg aaaccgtagc ccgtctgctg gggatcgggg cggtgcagcc
     5041 ggtttaccgc tttctggaca gcctggtggc gaaaggactg ctggcccggg cgaaataccc
     5101 ggttgacggt cgtcaggtca gcgtgtgggg gctgaccccg cacggcgtgg cgttctcgtt
     5161 cgacgaggac gagccgctga cggacgttat tccgtttcag ccctcgcggg tgtcggcggc
     5221 gcagctgccg caccggctgg ccgtgcagtc cctgcgtctt gatatggaag ccagaggggc
     5281 caccggctgg cgcaccctgc accgcctgag tcttaaaggt atgaaggtgc cggatgcgct
     5341 ggcagagctg gacggtaaaa cggtggcgtt tgaggtggag cgcaccgtca agagccgtcg
     5401 ccggtatcag gaagtggtgt ccgggtatct gttcaaccgt aaggcgaacg gcatcgatga
     5461 aatctggtac atctgccccg accggccgac gcttgtccgg gtacagcggg cgattctggc
     5521 ggtggatgaa atcgtgaacc cgcagaccgg cgaagcccgc aaaacggcgg aactggaccg
     5581 ggagaggctg ttcgcgtgct ttaaatttat gacaacagag tgagggatta tggcgctgta
     5641 catggttttt attgaggatg tggaagacaa agacggcgag cgctgcatta tgccaaccag
     5701 cgagcatctg gaagacatgg cggtcatggc ctggcagacc ggggcatatg tattccggtt
     5761 tgaagatcag gcccagcatc tggcgatgat gaagtttatc ggcatgtatc acgaacggct
     5821 gaacagcgat gacaaagcca cgtattactt caccggcaaa aatccggcgt gggaacgtga
     5881 taaaccgtcc ggaatgaagc tgtgtgatta tctttaccgc aaggttgagc agttcgccat
     5941 cgagaacggc gtgcaggtac tggacgcagg gaagtacacc ccggagcaga tcggcgcgat
     6001 gattgacgtg gtgagtgagc ggcacggtga gagtgcggtg aacgagaagc tgaaagcgga
     6061 gctgatgcga gagtataaca gccggtattt taacgagccg gtgcccgtat tgccggagga
     6121 taaacccgcc cgccggtggt ggccgttcgg caaaaaataa acgccagcac cggccatgcc
     6181 ggtgctgtta ttcttccccg agcttacccc gcattaccag ccagagtttt tccgcatcct
     6241 cgtgaagtcg ttcgcacatg tcggccgggt cgtccatgtc cagctcgttc agcttttcca
     6301 gcaacagcag cgactgatcc ctgatgaatt ttgccatgtt gagggcggtt ttttcctgct
     6361 tcgtcatgat tttttccctt ccgggcatgg tgtgcgccag tcgggtatac tgtctcaaaa
     6421 atagcggggt gagacaatgg cgtgggtgac ggtgagacag gcgacggaac tgacggggaa
     6481 agcccggagt tccctgtacc gggatatggc gaaaggccgc gtatcatacc ggacggaggc
     6541 tgatggcggt cgggttgtgg ataccagcga gcttatccgg gtgtacggtg agttaagaca
     6601 gggtgagact cacgagcggg acgggttgag actgccagat gagactgaaa aaacggcctc
     6661 tgaagcgttg atcgcggaga taaaagcact cagggaggag gtggccgggt tgcggcagga
     6721 aatgcaggcc atgagactgc tggaacacaa accggagacc gtagagacac cggccgaaac
     6781 gccgcgccgg tggtggcatt ggggaaaacg ctgacgcgct gcgcttgtcc aaccggcgac
     6841 gggcacgcaa tgaattgcgc ccctgtcacc ggttattagc gaacggtatg gggattctgg
     6901 cgctggccga ccacggccag aacccggact ttctgcgcgg ggtcgtccac ttcatagagg
     6961 acatgttgtc ccatcaggct gatccggcga acgtcttcgc cataacgtga gtccacttcg
     7021 tagaggcgcg ggaagtccgc cagtttgtcc gcagcagcga gaacccggtc ggtaacgttc
     7081 tgcgcatccc acagcgatga attttccgcg atatagtcaa acagtgtcgt tacatcttcg
     7141 atggctccct gcttccattc cacgtcatac ggcatcgcgc agttcctgat aacggccatg
     7201 caggcgctgg cggagttgtt ccgtggtcat gcccggacgc gtgtcagcac gggcggcggt
     7261 cactttggcg cgaacctgat cttcatactc tgcctgggcc agttcttcgt tagccaggcg
     7321 ctgcaacagc gcagctttgt ccgggccacc ttccggcagt atgcggggca ggctttcagc
     7381 cagttcccga agaaagttgg cgttttcgta gtgtgtcgtg ttgctcattg cggagcctcc
     7441 cgggttaatg cggcaatatg aaaaagttta gcaggaggcg gcctgaaaga atatgctcgt
     7501 gtcagtcagg ctgatgaagc agaaagtcgg taaaaaaggg cttacggcat ccattttacg
     7561 tcaaatccca tgccatgcag ttacgtgcat agccatgcgt tcaggtacaa tttatccggc
     7621 gcgtttccgg gggtgttgcg ggtggtttgt tgtctgtttt atcggtttcc cgtcagaaat
     7681 gccctcagag gctctcaggg ggtgcgggca acggatgtta tggtaaatga cgggattgtg
     7741 aggcatccag ctgacgattt ttgcgtcaac ttttcgctgt atgtttaaac atgagtttaa
     7801 cattagcgtc atctgatagc gctgttgcta atttcatcta cccgacaggt aatttccttg
     7861 taaatcaagc gcgaaagatc agcaaaaaag ctatgtgtgc gcttatggaa tagggtagaa
     7921 taagggattc tgatgatcgt gaggttgtca gtgaaaaacg aggcagctat cagatcttcg
     7981 gcctgagaaa ctaaggattc tgatagctgc cggtgactca gttcgcgaaa actgaatctt
     8041 acctgatcaa caaacgcccg attagggcgt ttgtcaagtg gtgctgcatt atgtagtaac
     8101 ttggtaccga cttgccaggc atttttcaca gttttttgat gtgcgaaggc tcatccggac
     8161 accgtcacct gttatttatc ctctgtaaac aggtgctata aatgcaaaaa gtaaccgatc
     8221 tgtatcctcc tgaaattgct cgccataagc tccagaatca cttcgccggt aataccgtta
     8281 tg
//
