LOCUS       NZ_CP110898            13038 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter freundii strain CF1807 plasmid pCF1807-7, complete
            sequence.
ACCESSION   NZ_CP110898
VERSION     NZ_CP110898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693425
            Assembly: GCF_026240835.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026240835.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:41:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,783
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,524
            CDSs (with protein)               :: 5,524
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 41 of 137
            Pseudo Genes (incomplete)         :: 93 of 137
            Pseudo Genes (internal stop)      :: 23 of 137
            Pseudo Genes (multiple problems)  :: 18 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110898.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CF1807"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCF1807-7"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2018-07-17"
                     /collected_by="zhangxiaofan"
     gene            18..2915
                     /locus_tag="OQW59_RS28845"
                     /old_locus_tag="OQW59_28845"
     CDS             18..2915
                     /locus_tag="OQW59_RS28845"
                     /old_locus_tag="OQW59_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            3661..4617
                     /locus_tag="OQW59_RS28850"
                     /old_locus_tag="OQW59_28850"
     CDS             3661..4617
                     /locus_tag="OQW59_RS28850"
                     /old_locus_tag="OQW59_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_208460129.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYASFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDDFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELTKDPISKKEDDSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            4625..5074
                     /locus_tag="OQW59_RS28855"
                     /old_locus_tag="OQW59_28855"
     CDS             4625..5074
                     /locus_tag="OQW59_RS28855"
                     /old_locus_tag="OQW59_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_208460128.1"
                     /translation="MALVSISEAARLTGKSRTTVQAYIKQGKLTKCTDSAGASKLDTSE
                     LLRVFGSFTGQQVGSEQSSIIVQHLAGESVQLTDQNLLHENYALKAEVELVKALLREKE
                     LLLNEKDKRNEDLKQALLLIESKLPATPKPATQPVEKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            5360..7435
                     /locus_tag="OQW59_RS28860"
                     /old_locus_tag="OQW59_28860"
     CDS             5360..7435
                     /locus_tag="OQW59_RS28860"
                     /old_locus_tag="OQW59_28860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_265970842.1"
                     /translation="MQTELIKELNKVLKVFPQFWSGENLHRSMVSDAISQKQPDLIKAL
                     VANKKIRSVYGTDINGTLIFDFDKLCSLLKYKEYWENSFTKYRNKIGLTSSGKYIADST
                     DIVLDFPFKDCLLEGGMTKEEQGKDEVYYNEIIARDEIDNLLSPKALVNVSRHTKDGEV
                     KNIHSFHEKDNLIIKGNNLIALHSLLGKFTGKVKLIYIDPPYNTGSDSFKYNDNFNHSS
                     WLTFIKNRLEVAKELLSEDGVIFVQSDDNEQAYLKVLMDGIFDRENFINMISVNMKNVA
                     GASGGGEDKRLKKNCEFILIYSKNYASLSAFKNVFEYTEIHEVVEQYRKEGKNWHYSSV
                     LVNAGEKIYVTSTVDGSGNEIKIYKHPNAVIKSVRQLMAQENISEKDVFYNFGTKIFEA
                     KDAQSSIRTRVAKAKKEHHVNDDILSIEYTPKSGKNKGQVYEQFYKGDKCRLFAWLRDI
                     TENIDGILYKKDKQGTYWDFTSHMNNLMKEGEISFQNGKKPEALIQRIIDISTNKDDIV
                     LDFHLGSGTTAATAHKMERRYIGIEQMDYINDITVPRLKNVIAAENNGISRDVSWQGGG
                     SFVYAELMELNAYFVHEIQKAQSTEELEKLFAVMKTEAHLNYQVALENVLSAEYEMDGI
                     PRKVAFSELELHEQKQLLIDILDKNQLYVNVSDMDDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            7440..10076
                     /locus_tag="OQW59_RS28865"
                     /old_locus_tag="OQW59_28865"
     CDS             7440..10076
                     /locus_tag="OQW59_RS28865"
                     /old_locus_tag="OQW59_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_265970835.1"
                     /translation="MAKKPKSAGSEKLLFNKLKQFDEPGLFQDSYQTPDYIQANLKDAL
                     RPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSQELEINGEKITIAPVDTFPSAPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKAKGKSKTSPENEEQTWE
                     RTIENILALRPDNRLFEYTATIDLANKDIGNKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDSEKMLDAVLLSQYRKLIAIDNGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESVRRHLANKRVQLSSDTSIWHKVIQRYAGSDLVTVIGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTGTDSEAQL
                     IGRGARYFPFVYDGKRSFTRRFDTSAKELSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTIEHARLKEDFKKSSVYQTGKLYFNEVEEIESSSRNWETYSLETRFEIPYQT
                     AGEESLDNLTGATAGITKSEPLLLDERFYRKAMQRISFYALDNLQRLFPKLTGIREFIR
                     SESYLGKLKITVTVPQSLDFAIVPAKEKLHLLETVLLRIAENMRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVVINQKITAVPTIGKKWYVYDNAILNQLEHRLVKTLEAFIPKL
                     RARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTCHADNAYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNEGESLET
                     ASLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(10119..10703)
                     /locus_tag="OQW59_RS28870"
                     /old_locus_tag="OQW59_28870"
     CDS             complement(10119..10703)
                     /locus_tag="OQW59_RS28870"
                     /old_locus_tag="OQW59_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_152954525.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVISLTEQFQDASAQLQQQSETLSR
                     LIRELDSASGNMAETVRKSVNSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(10716..11813)
                     /gene="mobA"
                     /locus_tag="OQW59_RS28875"
                     /old_locus_tag="OQW59_28875"
     CDS             complement(10716..11813)
                     /gene="mobA"
                     /locus_tag="OQW59_RS28875"
                     /old_locus_tag="OQW59_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_265970836.1"
                     /translation="MASYHLSVKTGGKGRASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQKERER
                     QQEKERQRLAEHTQQKPDKGWSFSR"
                     /name="mobA"
     gene            11978..12370
                     /locus_tag="OQW59_RS28880"
                     /old_locus_tag="OQW59_28880"
     CDS             11978..12370
                     /locus_tag="OQW59_RS28880"
                     /old_locus_tag="OQW59_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265970838.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLIMAGLV
                     DSQTGKPVDDTAALLGSVPAEGEMTP"
                     /name="hypothetical protein"
     gene            complement(12367..12972)
                     /locus_tag="OQW59_RS28885"
                     /old_locus_tag="OQW59_28885"
     CDS             complement(12367..12972)
                     /locus_tag="OQW59_RS28885"
                     /old_locus_tag="OQW59_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_265969940.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDHSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          3938..4617
                     /name="rep_cluster_1787"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10720..11813)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.041"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagggacg aatttgtatg tcacgacgcc atattttcac cgaacggcag cgagcagcgc
       61 tgttcgatct gcccacggac gaactgtcgc tactgaagtt ctacacgctg ggcgatgatg
      121 acctggaaaa cattaggcag cgccgcagac cggaaaacag gattggcttt gccctgcaac
      181 tttgtgcctt acgatatccg ggccgtgcac tggctcctgg tgagatgatc ccgcgtgaag
      241 tcctttcctt cgtcggtgct cagcttggag ttccggctga tgcgcttctc acttatgcca
      301 cacggcgcca aacccgtcag cagcacatgg acacgctgcg cgaaatttac ggctacaaga
      361 ccttcacggg ccgtggtgcc cgtgatctgc gggagtggac tttcggccag gccgaagatg
      421 ccagatcaaa cgaggatctt gctcatcgtt ttattgtgcg gtgtcgggaa acttccacca
      481 ttctgcccgc agtatcgaca atcgagcgct tgtgcgcgga tgctctggtc gccgctgagc
      541 ggcggattga aacgcggatt gcggaaaatt taacagcgga tgttcgcgat cacctggaca
      601 aacttctgag tgaaatgctc gccggcaata tcagtcgttt catctggctt cgcaacttcg
      661 aggttggtaa caactcggct gctgctaacc gtttgctcga caggctcgaa tttctgcgta
      721 ccctgaatat caatcatagt gctttggcca gcatacctgc ccatcgcatt gcccggctgc
      781 gtcggcaggg tgaacgctac ttcaccgacg gtttgcgtga catcacttcg gaccgccgct
      841 gggcgatcct tgccgtctgt gttgtggagt gggaagcggc gattgctgat gccatagtcg
      901 aaacccatga caggatcgta ggaaaaacct ggcgggaagc gaagcgccag catgacgaaa
      961 caatttccgg ctctaaagcc acactcacgg atacgatccg taccttcacc gcgctgggag
     1021 cttcgttgct tgaggcccgc agtgacggaa ccccgctgga gatggctgtc gccagttcgg
     1081 ttgcatggga ccggctcgct caactggtag cgacagggac tcaactcagc aacacgctag
     1141 ccgatgagcc tcttgcatat gtcgggcagg gataccatcg ctttcgtcgt tatgcgcccc
     1201 gcatgttgcg ctgtctgaag ctcgaagccg cgccggtcgc cggaccattg gtagcagcag
     1261 ctttgtcgat cggagagatg aaaggtgttg catcgccaga aaggcgtttc ctgcggccca
     1321 gctccaaatg gaaccgtcat ttacgagctc aggaaaaagg agatacccgt ctttgggaag
     1381 tggcggtact ctttcacctc cgggatgctt ttcgttccgg agatgtctgg ctcgctcatt
     1441 cgcgccgcta tggtgacctc aagcaggtac tggtgccgat gatcgcggcg caggaaaatg
     1501 caaaactggc cgtgccttcc aacccacagg attggctggc agacagaaag gcgcgactca
     1561 cgatcgctct taagcggctg gcccgggctg cccgtaacgg cactattccg cacggtagca
     1621 tagaagatgg aacgttgcgg atcgacaggt tgacagcaga cgtgccggat ggtgccgagg
     1681 cactcatact ggatctgtat cgccgaatgc cgtccgttcg gattaccgac atgctgcttg
     1741 aagttgatgc agcccttggt ttcacagatg cgtttaccca tctgagaacc ggggctccat
     1801 gtcgcgaccg gatcggtctg ctcaacgtcc tgctcgctga agggctcaat ctgggcctgc
     1861 gtaagatggc ggaagctaca aacacgcatg attactggca gctctcacgc cttgcccgct
     1921 ggcatgttga aagcgaagcc atgaaccagg cattggcaat tgtggtggcc gcgcagggta
     1981 aactgccgat gtcacgcgtc tgggggatgg gcacgtcagc atcgagcgat ggtcagtttt
     2041 tcccgacagc gcggcatggc gaagccatga acatggtcaa tgccaaatat ggttctgttc
     2101 ccggcctcaa agcgtatact cacgtaagcg accagttcgc gccattcgct tgtcagtcga
     2161 tcccggcgac cgtgagcgag gcaccgtata ttctcgatgg actactgatg aacgaggtcg
     2221 gtcgccatgt tcgcgaacag tatgccgata cagcaggatt caccgaccat ttgttcggag
     2281 ccagtagcct gctcggctac aatctcgttc tgcgaatcag ggatctgcca tcgaagcggt
     2341 tgtacgtatt taatcccgat acgaccccca gggagttacg caagttggta ggtggaaaag
     2401 cccgggagga tcttatcgtt gcgaactggc ctgatatttt ccgttgtgcc gcgacgatga
     2461 ccgctggcaa aatcaggccc agccaactcc tgcgcaagct cgcttcttac ccacgacaaa
     2521 acaaccttgc agttgcgctt cgtgaagttg gtcgtattga acggaccctt ttcattattg
     2581 agtggatcct ggatacggac atgcagcggc gtgctcagat cggtcttaac aagggagagg
     2641 cccaccatgc gctcaaaaat gcgctccgta tcgggaggca gggggaaatt cgcgatcgca
     2701 cgacagaggg gcagcactac cgaatcgctg ggctcaattt attgactgcg gtgatcattt
     2761 actggaatac cgtccatctt ggtcatgccg tcacggagcg gcggaacgaa gggttggatg
     2821 ttccccctga atttcttccc cacatatccc cattgggctg ggcgcacatt ctactgactg
     2881 gcgaatatct ttggcccaag gaaccgaaag cttagggtgt catttcgccc tcagccggaa
     2941 ccgacccctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt
     3001 ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca
     3061 catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg
     3121 ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattccct
     3181 gccagagccg tgagcgaagc gaactgattt tcacagagat aacagcgatt caggtgccgg
     3241 acggtcggag acaaaaggaa tgttcagcgg gttgcccgag cttgcgaggg tgctatctca
     3301 gtgtttttgg gggttaaggt ctgttttgaa gagaagcaaa cagcgtttgc gacatccttt
     3361 tatcatatcg ctcaagtgac tcaggcgtgc agttatccac aaggctggga tctattcttt
     3421 tttatctttt tctattcttt ctttattcta aagattttaa gttattgtta aaaaacaaaa
     3481 aaaaacagga aaggttaagc aaaaattagg aaaggttaag caaaaattag tatttccctc
     3541 ccaaaggtta agtgaaaatt aggtaatcca cacaaccacg caaaaggtta tgccagtgga
     3601 attgacataa ccaatacgaa aggttatgtt aatttaaaat cacaaccaat ggttttagtc
     3661 atggctgaat tggtagtatt caaagcaaat gagcttgcgg ttagtcgtta tgacctaaca
     3721 gagcacgaaa cgaaactaat cctgttttgt gttgcaaagc taaaccccac attagaaaca
     3781 ccagatgaaa ctgaacgaac ggttaaattt tcatgctcag aatatgcttc atttatgggg
     3841 ataagttatg agaatgcgtg gggacgctta aatacatcaa cacgagaatt atttaagcgg
     3901 tctattgagt taatatatcc aacaggtgct gtatctatgc gtgtttttaa ttgggctgaa
     3961 tatgcagagt tcaataggga aacacaagaa gtaactttag tatttagtaa atatataatc
     4021 ccgttgctat ttcacctaaa gagattcatt aaatacaacc tcgagcatgt taagtctttt
     4081 gaaaacaaat attcaatgcg catttatgaa tggctactaa aagaactaac acaaagaaaa
     4141 acacataaag ccaatatttc aataagtatt gatgatttta aattcatgct aatgttagaa
     4201 aaaagctacc cggaatataa attactaaac cattgggttt taaaaccaat atcaaaagat
     4261 ttaaatacat atagcaatat aaaactatct attgataagc gcggtcgtcc tgctgatacg
     4321 ctgatctttc aagttgaact tgataaacaa attgaccttg tgactgagct aacaaaagat
     4381 ccaatatcaa aaaaagaaga tgattcaatc cgtttaacgc ctgaaaatcg tctgcatgag
     4441 gggctaaaaa caacattgca tgatgcttta actgcgaaaa tacaacttac tggatttgaa
     4501 gctaaattcc tgagtgacat gcagagtaag tatgatctca acggctcatt tacatggctg
     4561 actcaaaaac aacgagccac gctagagaaa attttagcca agtacggacg aatctgaggt
     4621 atctatggct ctagtatcaa taagtgaagc agcaagacta actggaaaaa gccgcacaac
     4681 agtacaggca tacattaagc agggaaaact gaccaagtgt acagatagcg caggcgcttc
     4741 aaaactagac acatcagagc ttctacgtgt tttcggttca ttcactggtc agcaggttgg
     4801 cagtgaacaa tcaagcatca ttgtgcagca cttggcaggc gaatctgtgc agttaactga
     4861 ccagaatttg ctacatgaaa actatgcttt aaaagctgaa gtagaactcg taaaggcatt
     4921 gttgcgtgaa aaagagctgc tactcaatga aaaagacaag cgcaacgagg atctcaagca
     4981 agcgttgtta ctgatagagt ccaagctgcc agcaacacca aaacctgcca cgcagccggt
     5041 ggaaaaaaaa tcatggcaat tctggaagaa atagaggttt cagcctgttt actggctgaa
     5101 accatacccc cgattttgat aacaaaccgg taacaccaaa acagcccatt ttcggggatt
     5161 aaaacccgct attttggacg ttccggcggg ttctggtgat gagtaacagg tcagtggtgc
     5221 gcgcaagatc ccttatgtta aataccacct gttttggtga ttttttccgc atctgcatca
     5281 gcagtatcgc tgccaggttt ctgctcagga cgcgatgccc tacaatcatt tcaatctcta
     5341 acagtcataa ggtaatgaca tgcagaccga attaataaaa gaactgaaca aggttttaaa
     5401 agtgtttccg caattctgga gcggagaaaa tttacaccgt tcaatggtga gtgacgccat
     5461 cagccaaaag cagccagatc taatcaaagc actggtcgct aataaaaaaa tacgttctgt
     5521 ctacggcaca gatattaacg gaactttaat tttcgatttt gacaaacttt gcagcctgtt
     5581 aaaatataaa gaatactggg aaaacagctt tactaaatac cgaaataaga ttggattaac
     5641 cagctcaggt aaatacattg ccgatagtac tgatatagtg cttgattttc ctttcaagga
     5701 ttgccttctt gaaggcggaa tgacaaaaga agaacaaggg aaagacgagg tttattataa
     5761 cgagattatt gctcgcgatg aaatagataa tcttctatcc ccaaaagcat tggttaatgt
     5821 atctcgccat acaaaggatg gggaagttaa aaacattcat agcttccatg aaaaagataa
     5881 cttgataata aagggaaata atttaattgc acttcactca ctattgggta agtttactgg
     5941 aaaagttaaa ttaatatata tagacccacc atacaataca ggatctgaca gttttaaata
     6001 caatgacaat ttcaatcatt catcatggct aacatttata aaaaatagac tagaagtagc
     6061 aaaagaactt cttagtgaag atggagtcat cttcgtacaa tcagacgaca atgaacaagc
     6121 gtatttaaaa gttttaatgg atggcatttt tgatagggaa aactttatca atatgatatc
     6181 ggttaacatg aagaatgttg ccggagcctc tggtggaggt gaggataaaa gacttaaaaa
     6241 aaactgtgag tttattttaa tttactcaaa aaactatgcc tccctttctg catttaaaaa
     6301 tgtttttgaa tacacagaaa ttcatgaggt agttgagcaa tataggaagg aaggtaaaaa
     6361 ttggcactat tcctccgtac tggtaaatgc aggggaaaaa atatacgtaa cctctactgt
     6421 tgatggcagt gggaacgaaa taaagatata caaacaccct aacgcagtaa ttaaatcagt
     6481 taggcaattg atggcgcagg aaaacatttc agaaaaggat gttttttata actttggaac
     6541 aaaaatattt gaagcgaaag atgctcaatc atccattaga acgcgagtag caaaagcaaa
     6601 aaaagagcat catgttaatg atgatatatt atcaatagaa tacacaccta aatcaggaaa
     6661 aaataaaggt caagtatatg aacaattcta caaaggggat aaatgtagat tgtttgcatg
     6721 gcttagagat ataactgaaa atatagatgg cattttatac aaaaaagata aacaaggaac
     6781 gtattgggat ttcacatcac acatgaacaa tctaatgaaa gaaggtgaaa tatcatttca
     6841 aaatggaaaa aaaccagagg cattaataca aagaattata gatatttcaa caaataaaga
     6901 tgatattgta ctagatttcc atctcggaag cgggacaaca gctgcaacag cacataaaat
     6961 ggaacgtcgt tacattggca ttgaacaaat ggattacatc aatgacataa cggttcctcg
     7021 actaaaaaat gtaatcgcag cggaaaacaa tgggatttca agagacgtga gctggcaggg
     7081 cggtggaagc tttgtatacg ccgaactgat ggagctgaac gcgtacttcg tgcacgaaat
     7141 ccagaaagcg cagtccactg aagaactcga aaaactcttt gctgtaatga aaaccgaggc
     7201 gcatctcaac tatcaggtgg cactggaaaa cgtactctct gctgaatacg agatggacgg
     7261 cattccgcgt aaagttgcgt tcagcgaact ggaactgcat gagcagaagc agctactgat
     7321 tgatatcctc gataaaaatc agctctacgt gaacgtttct gatatggatg acagtgacct
     7381 gaacatctca gagagcgata aagcctttac ccgcagcttc tacggaatgg agtaacggca
     7441 tggcgaagaa accaaagtca gccggcagcg agaagctgct gtttaacaag ctgaagcagt
     7501 tcgatgaacc tgggcttttc caggatagct accagacgcc ggactacatt caggcgaacc
     7561 tgaaggatgc cctgcgcccg taccagcatg gggcgctgcg ctacctgcat tacactcagc
     7621 gcaaacgtga agaggcgctg ctgcattacc gccatctgtt gtttcatatg gcaaccggtg
     7681 cagggaaaac gatggtgatg gcggggacga tactctatct gtttaaagag tacgggtatc
     7741 agaacttcat ctttttcgtg cataccgacg cgattatcca gaaaacccga gagaacctgc
     7801 tcaacccgca gtcgccgaag tacctgttca gtcaggagct ggaaatcaac ggggaaaaaa
     7861 tcaccattgc accggtggat acgttcccgt ctgcaccaga gcgcaacacg atttacctca
     7921 agttatcaac tatccataag atgcacgacg agctgaacag ctatcgtgag aacagcatca
     7981 cctacgagga tttaaaagaa ataccgctcg tcctgcttgg cgacgaggcg catcacttca
     8041 acgccggcac gaaagcgaaa ggcaagtcaa agacctcacc ggagaacgag gagcagacct
     8101 gggagcgcac gattgagaac atcctcgctc tgagaccgga taaccgactg ttcgagtaca
     8161 ccgccaccat tgacctggca aacaaagata tcggaaataa ataccgtgac aaagtggtgt
     8221 atcagtatga cctgaagcag ttcatgtcag acggttactc gaaaaaggtc atgctactgg
     8281 aagccaacca gaacgacagc gaaaaaatgc ttgatgccgt gctgttaagt cagtaccgca
     8341 aactgatagc gatagataac ggcatcaccg gcttcaagcc ggtcattctg ttcaaatcga
     8401 acaagattgc gatatcgaag gcgaagcagg aagagttttc gcaactgatt gcggcaatga
     8461 cccctgaaag cgtcagacgt catctggcga ataaaagagt ccagctcagc tctgacacca
     8521 gtatctggca taaagtgatt cagcgttacg ctggcagcga tttagtgacg gttatcgggc
     8581 agattcagga agattttaac gacttcaacc tgctgaacgt caataaatcc gacttgctgg
     8641 aagaaaaccc cgtcctgctg aatacgctgg aagaagtcga taatccggtg agagccgtct
     8701 ttgccgtggc gaaggtcaat gaaggctggg acgtgctcaa cctctacgac atcgtccgca
     8761 tcagcgaaca ggcgagtagc agcaaaaccg gcacggacag cgaggcacag cttatcgggc
     8821 gcggcgcgcg ttacttcccg ttcgtatatg acggcaagcg gagcttcacc cgccgatttg
     8881 ataccagcgc gaaggagttg agcgtgctgg aacaactgca ctatcacacc atcaatgaac
     8941 cggcgtacat caaaacgctg cacgcatctc tggagcaggc ggacattgac gtgcatcagg
     9001 acggcggcgg cacgattgag cacgccagac tgaaagagga ctttaaaaag tcctccgtat
     9061 atcagacagg taagctgtac tttaacgaag tggaagagat tgagagcagc agtcgcaact
     9121 gggaaaccta ctcgctggaa acgcgctttg agataccgta tcagaccgcc ggtgaggagt
     9181 cgttggacaa cctgacaggg gcaacggctg gcatcacaaa gtcagaaccc ctgctactgg
     9241 acgagcgttt ttatcgcaag gccatgcaga ggatcagttt ttacgcgctg gataaccttc
     9301 aacgcctctt cccgaaactg accggtattc gtgaattcat ccgcagtgag tcttatcttg
     9361 ggaaactgaa aatcaccgtt accgtaccgc agtctctgga tttcgccatc gtaccggcaa
     9421 aagagaaact gcacctgctg gaaaccgttc tgctgcgtat cgcagagaac atgcgccgca
     9481 atgaccagaa agtgaaaggt acgtaccgtt ttatcagcca gccggtgaaa gaggtgatta
     9541 aagactacag cctgcacatt gacccgtcgg tagtcattaa ccagaaaatc accgccgtac
     9601 ccaccattgg taaaaaatgg tacgtgtacg ataacgcgat actgaatcag cttgagcacc
     9661 gtctggtgaa aactctggaa gcctttatac cgaagctcag agcacgttac gacgatattt
     9721 acgtgctgcg taacgacgag cagtcaacgc gctttaagct cacggaattt ggcggcgtac
     9781 gtggctttat gccggacttc atcatgatac tgacgtgcca tgcggataat gcttactggc
     9841 aggtcttcct tgagccgaaa ggggatgacc gtctactgga tgacgcatgg aaagagcgga
     9901 tgctcgaaac cctgaatgac cgggaacgca ttgtaattga tgaaaatgag gacgtccggc
     9961 tggtcgggat taaattcttt gccaacagtc agatggatgc cttcgtcagt gatatgcaga
    10021 accggctgaa tgagggggaa tcgcttgaaa ccgcatcgct ctccctgcca ctgtaaacgg
    10081 tgacaaatac aaataacggc gcacagtgcg ccgttatttt attttttgat aatcatccag
    10141 gtattctctt tatcaccata cgtggtgcga gtgtccacct taacgcaggg ctttccgtca
    10201 caacgcgata tatcagctag cggggctttc ttctgccaga cggcttccat ccgctgcatt
    10261 tcagcaatct ggctgtaccc gtcattcata aaccacgtga atgccgtcac gcaggcagcc
    10321 agaacgaaga atatcgtcag cacaagataa atcgcggatt tccacgtata gcgcgacatc
    10381 tcgcggcgca tttcatggat cattgccttc gccgtatcgg cagcctgatt aagcgcttct
    10441 gtcgctggtt tctgctgtgc taatccggct tgtttcagtt ctttctcaac ctgagtgagc
    10501 gcggaattaa ccgatttcct gacggtttca gccatattac cggacgcgct gtccagctca
    10561 cgaatgagcc tgctcagcgt ttcactctgc tgctgtaatt gtgctgaggc gtcctgaaac
    10621 tgctcagtca gagagataac acgcttttcc agcgcctgat gatgcccgat aagggcggcg
    10681 atttgtttaa tttcgtcact catgcaaaat cctgtctatc gtgaaaatga ccagccttta
    10741 tccggcttct gttgtgtatg ttcggcaagt ctctgccgtt ctttctcctg ctgacgctcg
    10801 cgttctttct gtcgttccat ttcctgatgt atcccctgaa actccgccat tgcatcgtta
    10861 accagggact gaaggtcgat ttcttcctgt atatccttca tggcatcact gaccagtgcg
    10921 ttcagcttgt caggctcttt ttcaaaatca aacgttctgc cggaatggga ttcctgctca
    10981 ggctctgact tcagctcctg ttttagcgtc agagtatccc tctcgctgag ggcttcccgt
    11041 aacgaggtag tcacgtcaat tacgctgtca cgttcatcac gggactgctg cacctgcctt
    11101 tcagcctccc tgcgctcaag aatggcctgt agctgctcag tatcgaatcg ctgaacctga
    11161 cccgcgccca gatgccgctc aggctcacgg tcaatgccct gcgccttcag ggaacgggaa
    11221 tcaacccggt cagcgtgctg ataccgttca aggtgcttat tctggaggtc agcccagcgt
    11281 tccctctggg caacaaggta ttctttgcgt tcggtcggtg tttccccgaa acgtgccttt
    11341 tttgcgccac cgcgctccgg ctctttggtg ttagcccgtt taaaatactg ctcagggtca
    11401 cggtgaatac cgtcattaat gcgttcagag aacatgatat gggcgtgggg ctgctcgcca
    11461 ccggctatcg ctgctttcgg attatggata gcgaactgat aggcatggcg gtcgccaatt
    11521 tcctgttgga caaaatcgcg gacaagctca agacgttgtt cgggttttaa ctcacgcggc
    11581 agggcaatct cgatttcacg gtaggtacag ccgttggcac gttcagacgt gtcagcggct
    11641 ttccagaact cggacggttt atgcgctgcc cacgccggca tattgccgga ctccttgtgc
    11701 tcaaggtcgg agtctttttc acgggcatac tttccctcac gcgcaatata atcggcatga
    11761 ggagaggcac ggcctttccc gccggttttt acgctgagat gataggaggc catcgtgttt
    11821 ttatcccgct gaaggcgcgc accgtttctg aacgaagtga agaaacgtct aagtgcgccc
    11881 tgataaataa aagagttatc agggattgta gtgggatttg acctcctctg ccatcactga
    11941 gcataatcat tccgttagca ttcaggaggt aaacagcatg aataaaagcg aaaaacagga
    12001 acaatgggca gcagaaagag tgcagtatat tcgcggctta aagtcgccga atgagcaaca
    12061 gaaacttatg ctgatactga cggataaagc agataaaaca gcacaggata tcaaaacgct
    12121 gtccctgctg atgaaggctg aacaggcagc agagaaagcg caggaagcca gagcgaaagt
    12181 catgaacctg atacaggcag aaaagcgagc cgaagccaga gccgcccgta aagcccgtga
    12241 ccatgctctg taccagtctg ccggcttact tatcatggcg ggactggtag acagccagac
    12301 agggaagccc gtcgatgata ccgctgcctt actggggtcg gttccggctg agggcgaaat
    12361 gacaccctaa gcgttagctc tgtgtcgttg cacgatgtca gcgacggtat tcttgctgat
    12421 accgagctcg cgtgcgatcc agcgatagct gcgtccctcg gccctcatcg caaccacctt
    12481 aggcaaaagt cggtctgatt ttggtcgcac tccggcctga cgaccaagcc tcttaccacg
    12541 tgccttcgca acagcaaggc ctgacttgac ccgctcgctg atgagatccc gctcaaactc
    12601 cgcaatgccg gaaagaaacg tcgccagcat tcgtccatac ggcgacgaat gatcgaacgc
    12661 cattccattc atggctatca cggaaacctt ccagttctcc agttcacgta gcgtattgag
    12721 cagatcgagc gtcgagcgcc cccaccggga aagctcagtg accaggattg catcaatttg
    12781 tctggactgg gcaagcgcca ggactttctt tcgctcggcc cggtcgagtt tagttcctga
    12841 acctgtttcc ttaaatattc ccaccacgtc gtagccggca cggccggcga aggctcgcag
    12901 atcaaattcc tggcgttcac aagactgatc cgctgttgaa acccggcagt aaatggcggc
    12961 acgatgtccc aattgaaccc tcctggattt ttgtatcgga acgccctgat ttatatgggc
    13021 tggctgttgt ccaaaaca
//
