LOCUS       NZ_CP110897             8282 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter freundii strain CF1807 plasmid pCF1807-8, complete
            sequence.
ACCESSION   NZ_CP110897
VERSION     NZ_CP110897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693425
            Assembly: GCF_026240835.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026240835.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:41:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,783
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,524
            CDSs (with protein)               :: 5,524
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 41 of 137
            Pseudo Genes (incomplete)         :: 93 of 137
            Pseudo Genes (internal stop)      :: 23 of 137
            Pseudo Genes (multiple problems)  :: 18 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CF1807"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCF1807-8"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2018-07-17"
                     /collected_by="zhangxiaofan"
     gene            complement(join(8181..8282,1..276))
                     /locus_tag="OQW59_RS28790"
                     /old_locus_tag="OQW59_28790"
     CDS             complement(join(8181..8282,1..276))
                     /locus_tag="OQW59_RS28790"
                     /old_locus_tag="OQW59_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            346..525
                     /locus_tag="OQW59_RS28795"
                     /old_locus_tag="OQW59_28795"
     CDS             346..525
                     /locus_tag="OQW59_RS28795"
                     /old_locus_tag="OQW59_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(553..1083)
                     /locus_tag="OQW59_RS28800"
                     /old_locus_tag="OQW59_28800"
     CDS             complement(553..1083)
                     /locus_tag="OQW59_RS28800"
                     /old_locus_tag="OQW59_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1090..1821)
                     /locus_tag="OQW59_RS28805"
                     /old_locus_tag="OQW59_28805"
     CDS             complement(1090..1821)
                     /locus_tag="OQW59_RS28805"
                     /old_locus_tag="OQW59_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(1821..3782)
                     /locus_tag="OQW59_RS28810"
                     /old_locus_tag="OQW59_28810"
     CDS             complement(1821..3782)
                     /locus_tag="OQW59_RS28810"
                     /old_locus_tag="OQW59_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5240..5572)
                     /locus_tag="OQW59_RS28815"
                     /old_locus_tag="OQW59_28815"
     CDS             complement(5240..5572)
                     /locus_tag="OQW59_RS28815"
                     /old_locus_tag="OQW59_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321410.1"
                     /translation="MEKQYTNELTAEILAGMDQSPFTPEQLAAMSDEARALIEEQEAFC
                     HAHPVTTIYRLAVAGCLTRRGGTGDEFNPNPEEGHKIRLENGLWVSVLTEGCTVTYPDG
                     TQARIL"
                     /name="hypothetical protein"
     gene            5726..5977
                     /locus_tag="OQW59_RS28820"
                     /old_locus_tag="OQW59_28820"
     CDS             5726..5977
                     /locus_tag="OQW59_RS28820"
                     /old_locus_tag="OQW59_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321409.1"
                     /translation="MALVQKKTLNELATNASDDFFGKRFSAGDEVPHSGIYKCTKCNRE
                     VTVNAHSRDNTFPPHYPESPCKSPTWKPYVIADTKAEW"
                     /name="hypothetical protein"
     gene            5990..6244
                     /locus_tag="OQW59_RS28825"
                     /old_locus_tag="OQW59_28825"
     CDS             5990..6244
                     /locus_tag="OQW59_RS28825"
                     /old_locus_tag="OQW59_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321408.1"
                     /translation="MNFKQLIPAQDWYFRHFTDQSDYPVIVYQLAAWALTEEGSITGLV
                     AIYDPKEGKAKLVTPPPVKGNYLHRDQLTDEELAASRKP"
                     /name="hypothetical protein"
     gene            complement(6275..6793)
                     /locus_tag="OQW59_RS28830"
                     /old_locus_tag="OQW59_28830"
     CDS             complement(6275..6793)
                     /locus_tag="OQW59_RS28830"
                     /old_locus_tag="OQW59_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704828.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVARSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            7567..7830
                     /locus_tag="OQW59_RS28835"
                     /old_locus_tag="OQW59_28835"
     CDS             7567..7830
                     /locus_tag="OQW59_RS28835"
                     /old_locus_tag="OQW59_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7820..8119
                     /locus_tag="OQW59_RS28840"
                     /old_locus_tag="OQW59_28840"
     CDS             7820..8119
                     /locus_tag="OQW59_RS28840"
                     /old_locus_tag="OQW59_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(4777..4956)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.659"
                     /coverage="90.0"
                     /evalue="1.1199999999999999e-60"
     Typing          complement(1094..1821)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.12e-141"
ORIGIN
        1 caacccggcc acctcctccc tgagtgcttt tatctccgcg atcaacgctt cagaggccgt
       61 tttttcagtc tcatctggca gtctcaaccc gtcccgctcg tgagtctcac cctgtcttaa
      121 ctcaccgtac acccggataa gctcgctggt atccacaacc cgaccgccat cagcctccgt
      181 ccggtatgat acgcggcctt tcgccatatc ccggtacagg gaactccggg ctttccccgt
      241 cagttccgtc gcctgtctca ccgtcaccca cgccattgtc tcaccccgct atttttgaga
      301 cagtataccc gactggcgca caccatgccc ggaagggaaa aaatcatgac gaagcaggaa
      361 aaaaccgccc tcaacatggc aaaattcatc agggatcagt cgctgctgtt gctggaaaag
      421 ctgaacgagc tggacatgga cgacccggcc gacatgtgcg aacgacttca cgaggatgcg
      481 gaaaaactct ggctggtaat gcggggtaag ctcggggaag aataacagca ccggcatggc
      541 cggtgctggc gtttattttt tgccgaacgg ccaccaccgg cgggcgggtt tatcctccgg
      601 caatacgggc accggctcgt taaaataccg gctgttatac tctcgcatca gctccgcttt
      661 cagcttctcg ttcaccgcac tctcaccgtg ccgctcactc accacgtcaa tcatcgcgcc
      721 gatctgctcc ggggtgtact tccctgcgtc cagtacctgc acgccgttct cgatggcgaa
      781 ctgctcaacc ttgcggtaaa gataatcaca cagcttcatt ccggacggtt tatcacgttc
      841 ccacgccgga tttttgccgg tgaagtaata cgtggctttg tcatcgctgt tcagccgttc
      901 gtgatacatg ccgataaact tcatcatcgc cagatgctgg gcctgatctt caaaccggaa
      961 tacatatgcc ccggtctgcc aggccatgac cgccatgtct tccagatgct cgctggttgg
     1021 cataatgcag cgctcgccgt ctttgtcttc cacatcctca ataaaaacca tgtacagcgc
     1081 cataatccct cactctgttg tcataaattt aaagcacgcg aacagcctct cccggtccag
     1141 ttccgccgtt ttgcgggctt cgccggtctg cgggttcacg atttcatcca ccgccagaat
     1201 cgcccgctgt acccggacaa gcgtcggccg gtcggggcag atgtaccaga tttcatcgat
     1261 gccgttcgcc ttacggttga acagataccc ggacaccact tcctgatacc ggcgacggct
     1321 cttgacggtg cgctccacct caaacgccac cgttttaccg tccagctctg ccagcgcatc
     1381 cggcaccttc atacctttaa gactcaggcg gtgcagggtg cgccagccgg tggcccctct
     1441 ggcttccata tcaagacgca gggactgcac ggccagccgg tgcggcagct gcgccgccga
     1501 cacccgcgag ggctgaaacg gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa
     1561 cgccacgccg tgcggggtca gcccccacac gctgacctga cgaccgtcaa ccgggtattt
     1621 cgcccgggcc agcagtcctt tcgccaccag gctgtccaga aagcggtaaa ccggctgcac
     1681 cgccccgatc cccagcagac gggctacggt ttcagccgtc gagcagagtt cgtcccgcag
     1741 ccaccgcagg actagccggg ttttctccgc gttccgcgcc tgtcgcttcg cgtggctgac
     1801 gttgtattgc tccagagcca tcagtacagc cccgcaaaat catcgtcacc cgggcgctgc
     1861 cgcactgcct cagcctggta ctccgccagc gggtccgttt tcgccagcac cgggaaggtt
     1921 ttcagcggaa tttcccggcg atcaacccgc agcgggctgc tgaacgccag ccgcgccacg
     1981 cccagcccgg tcatgaccgc gaacccgtcc ggcaggtgtt gcagggtgtt cacgtcgaac
     2041 agcggccgcg cttccttctg gatgaagctg tccccgctga tgtgctccac gttccccgcc
     2101 tcccggctgg cccggcggcg ctccacgtcc acccggattt ccccggtctg tccggccgcc
     2161 cactgcgcgg actcctggct ggccgcgcga tagaaccagc ggattggcgt gttatccagt
     2221 accgtggttt tcacaaaatc agcgggcaga tcctgcccgc actggccgaa gtcgcccagc
     2281 gactggtgag ccagcagcag gttacagtcc cggctgcgca gcgtccccag cgcgttgagt
     2341 acgtacttcg agagcagata tttgatttca tcgagcatca cgctgacgtg cggccacgcg
     2401 cggaactcgt cccgcgcaat gatgatttgc gcgcagcggg caaacagcat tttctgcacc
     2461 cggatcaccg cctcgtcgtc catcgatccg atgacgtaca gacagccgcc gccgttgata
     2521 atccccgcca catcaatacc ggaatccgtc tgcaggacgc tcaggtccgc gacgttctcc
     2581 agcttggtga tcagcccctt cacgtccttt ttcagcgcct ccggcagcgc atacgccgcc
     2641 gccagcacct gctgaatgtt cgcgccgtgc gggaactgct ccgcgataag cttcgccgct
     2701 ttctgctcgc tgatgcggta gtggtcggcc acatcgccgg tttccgccag gttaaacccg
     2761 gcaaccagca gatttttcag tgcgtactgg tcaaggtcgc ggaacaggtt cagctgcgcc
     2821 ggtttccccg gccgcaggtc cagcagggtg aagtccgggc agtgcgcccg aaagacgtgt
     2881 ggcgcccatg cgtcgccttt cgggtcgaag tacaccaccg catcgccaaa atcgcgcacg
     2941 cagcggatca gcgcgttggt ggccatcacg ctcttgccgc tcccgggtag gccgaggatc
     3001 tgaatattgg tcttgcgcca cgcctcgcgc ggaacccgca ccggctggcc gtcctcgtcc
     3061 ttacccagga acaccacctg ctgctgccgg gcctgctccg tggaaaaatc ggccagcgcg
     3121 ttgaccggca ccggctcccc gaaatgcagg tggcgcacgt cagtcatttc gccggtgcgc
     3181 tggctgctgc gccggctctc ctgattcagt tttgcattga ccgcctcgtc agcggccgca
     3241 aaatgaccgt caagcagcag gaatgccagt agccgcgcca gaagtatccc gacgaccaga
     3301 ccaccggcac accaggctat cccgaacaga ttagcgggtt gcagcaggta ataatcggca
     3361 ttcgcgtagt agcccggacg caacaccgtt tctccccagc tctcgataaa cacggcgaca
     3421 accagccccc ccagcagaac cagaccggca gcgccggtca gccagtacgc gtagttccag
     3481 acatgtcgac gagccctgac catccgcccg ggagaatcca gcgaggccag caccgaaaat
     3541 ggcgcagccc tgtcattatc gcgcttatag cgatcggcat actcccgccg ctccttatgg
     3601 ctcaggccca caaaacgcat ccgtccggcc cagagttcag ccagaccgaa aaacaggccg
     3661 ataaccagaa aagccccggc cagaccgtaa tactcccggt acagccagct gacccacggg
     3721 taaatctgcc gggccagctc cgggatatgc cactgccagg caagcgaatc cgtattcaac
     3781 atgcaacctc tctcctacag acgacggatt taccggcgtg atgcgcagcc catctgcgca
     3841 agctgacgac atgccccaag gcatggcggc ggcgttcacg tccgtggtga aagcgttcag
     3901 cgctcgccac ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga
     3961 acaacgagca cgcccacagc gtgcgagagg aaaaggcggc accccagtgc tgtcttttcc
     4021 ggtgttccgg cccccgtcag agtaagcgac agcgagcggc tgaccaccca taaaatcagg
     4081 agcagctttg ctgcttcgtc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc
     4141 gtgagcctga cgggggccgg gtgaatttct ggcgtgaccg gcgtccagcc gggctaagac
     4201 agaaagaggg gtaaagacgc tgcgtcggtg gcgttcagcc agcgtaagca ggggatttac
     4261 ccctgaagtc agtcccgccc tagcggggat gacttgcatt tctccgcaag gagaaaaaga
     4321 aacagagata ggttaacgta aaatgtgtat cagaaaaccg acatacttca cctatcctga
     4381 tgatttatcg acagggaaca gaaaggcttt aatcaggagc aaaatgtcac atcagcgcgt
     4441 ccggtggccg taggcaaaaa cgggagatat tgcgcctcct cgctcactcg gtcgctacgc
     4501 tccggccgtg aaactgcggc gggcgctgcg gactcacgag cagggcaaac aggcatagag
     4561 agaaaataat acgaagctgc gccggtggcg tttttccata ggctccgccc tcctgccaga
     4621 gttcacataa acagacgctt tttaggtgca tctgtgggag ccgtgaggct caaccatgaa
     4681 tctggcagta cgggcgaaac ccgacaggac ttaaagatcc ccacagtttc cggtgggtcg
     4741 ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc
     4801 tcccttgcgg gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt
     4861 aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg
     4921 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg
     4981 cagcagccac tggtaactgg agttcgcagg aacgtaagag tgaaaaagta actttttcgc
     5041 ttgaagaagc cagtttgaag cagctaaact gactgtactg ttttggtgac tgttcctgcg
     5101 aagatcagtt accgcccggt ttcggcgagt ttgccgctcg acgaacccga actaacccgc
     5161 cttacggcgg gttttttcgt ttacagagca agagattacg cgcagtggta aacgatctca
     5221 agtggatctt tttaagatcc tacagaatcc gggcttgcgt gccatcagga tacgtcacgg
     5281 tacaaccttc agtcagcacg ctgacccaca acccgttctc cagccggatt ttatggcctt
     5341 cctcagggtt cggattgaac tcatcgccgg taccaccgcg ccgggtcaga cagcccgcca
     5401 ccgccagtcg gtaaatggtc gtcaccggat gcgcatggca gaatgcctcc tgctcctcaa
     5461 tcaacgcgcg tgcttcatcg ctcatcgcag ccagttgttc aggagtaaaa ggtgattgat
     5521 ccattccggc cagaatctcc gcggtcagtt cattggtgta ctgcttctcc atactaattt
     5581 cttgtacttt aaatggttaa aatcacgcca tcatactcat aatcctgact taaccaaaac
     5641 tcagccgctt gaaaaaacgc cacaaagacc acatttataa aatgggtaag aatgcccgaa
     5701 aaatatcgat gggaaggaaa gcagaatggc cttagtacag aaaaaaacgc tgaatgaact
     5761 ggcaacaaac gccagcgatg acttcttcgg taagcgattt tcagccggag atgaagttcc
     5821 acactcgggg atctacaaat gcacgaaatg taatcgtgaa gtgacggtaa atgcccactc
     5881 cagagacaat acatttccgc cacattaccc ggaaagcccg tgtaaatcac cgacgtggaa
     5941 accctatgtc attgcagaca caaaagcgga atggtgaagg agctaataca tgaactttaa
     6001 gcaactcatc cccgcacagg actggtattt tcgtcatttt accgaccagt ctgattatcc
     6061 ggtgatcgtt tatcagcttg ctgcatgggc actgactgag gaaggaagca ttaccggcct
     6121 tgtggcgata tacgatccga aagaaggcaa agcaaaactg gtgacaccac cgccggtaaa
     6181 agggaactat ctgcaccgag atcagctgac agatgaagag ctggcggcat ccagaaaacc
     6241 ttaaaaataa agcccctgag gggctttact ggtatcagct tgcagggtgt gcgcctggcg
     6301 gcggtgtacc gttcgaccgg gcaacattgc gtaacagcgc cagtatgccg tcttcaccaa
     6361 actccttgta caaccgactc atttccttct gagcattggt tcgcttctgg ctgttcaggt
     6421 tacgttcgca ctctttcatc atcttttccg gctctgattt cagccttgca agatcgtctt
     6481 catcgataca gaaagccgcc tgctttaccg caccacctac atcggcctta gtcgaaatcg
     6541 ttctggccgt aaccggcaga tatttttgct taagcgaatg ggccacggct gacgcacggt
     6601 taacacacag atcggccgta atgttatagc cggaagtggt cacgacgttg ccgtcacaga
     6661 gcgccgcaaa agtacacagg ctggttttat ttagtttctg gattaactcc agcataacgg
     6721 tattaccggc gaagtgattc tggagcttat ggcgagcaat ttcaggagga tacagatcgg
     6781 ttactttttg catttatagc acctgtttac agaggataaa taacaggtga cggtgtccgg
     6841 atgagccttc gcacatcaaa aaactgtgaa aaatgcctgg caagtcggta ccaagttact
     6901 acataatgca gcaccacttg acaaacgccc taatcgggcg tttgttgatc aggtaagatt
     6961 cagttttcgc gaactgagtc accggcagct atcagaatcc ttagtttctc aggccgaaga
     7021 tctgatagct gcctcgtttt tcactgacaa cctcacgatc atcagaatcc cttattctac
     7081 cctattccat aagcgcacac atagcttttt tgctgatctt tcgcgcttga tttacaagga
     7141 aattacctgt cgggtagatg aaattagcaa cagcgctatc agatgacgct aatgttaaac
     7201 tcatgtttaa acatacagcg aaaagttgac gcaaaaatcg tcagctggat gcctcacaat
     7261 cccgtcattt accataacat ccgttgcccg caccccctga gagcctctga gggcatttct
     7321 gacgggaaac cgataaaaca gacaacaaac cacccgcaac acccccggaa acgcgccgga
     7381 taaattgtac ctgaacgcat ggctatgcac gtaactgcat ggcatgggat ttgacgtaaa
     7441 atggatgccg taagcccttt tttaccgact ttctgcttca tcagcctgac tgacacgagc
     7501 atattctttc aggccgcctc ctgctaaact ttttcatatt gccgcattaa cccgggaggc
     7561 tccgcaatga gcaacacgac acactacgaa aacgccaact ttcttcggga actggctgaa
     7621 agcctgcccc gcatactgcc ggaaggtggc ccggacaaag ctgcgctgtt gcagcgcctg
     7681 gctaacgaag aactggccca ggcagagtat gaagatcagg ttcgcgccaa agtgaccgcc
     7741 gcccgtgctg acacgcgtcc gggcatgacc acggaacaac tccgccagcg cctgcatggc
     7801 cgttatcagg aactgcgcga tgccgtatga cgtggaatgg aagcagggag ccatcgaaga
     7861 tgtaacgaca ctgtttgact atatcgcgga aaattcatcg ctgtgggatg cgcagaacgt
     7921 taccgaccgg gttctcgctg ctgcggacaa actggcggac ttcccgcgcc tctacgaagt
     7981 ggactcacgt tatggcgaag acgttcgccg gatcagcctg atgggacaac atgtcctcta
     8041 tgaagtggac gaccccgcgc agaaagtccg ggttctggcc gtggtcggcc agcgccagaa
     8101 tccccatacc gttcgctaat aaccggtgac aggggcgcaa ttcattgcgt gcccgtcgcc
     8161 ggttggacaa gcgcagcgcg tcagcgtttt ccccaatgcc accaccggcg cggcgtttcg
     8221 gccggtgtct ctacggtctc cggtttgtgt tccagcagtc tcatggcctg catttcctgc
     8281 cg
//
